Restriction map for pF1KA0808
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# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AhdI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BanI        BbeI        
BbrPI       BcgI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       FseI        FspI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AflIII        116  ACATGT               A^C[GA][CT]GT
AgeI         1509  ACCGGT               A^CCGGT
Alw44I        250  GTGCAC               G^TGCAC
AlwNI        1214  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1081  GGGCCC               GGGCC^C
ApaLI         250  GTGCAC               G^TGCAC
BalI          528  TGGCCA               TGG^CCA
BamHI         768  GGATCC               G^GATCC
BbvCI        1045  CCTCAGC              CCTCAGC(-5/-2)
BciVI         997  GTATCC               GTATCC(6/5)
BclI         1052  TGATCA               T^GATCA
BcnI         1135  CCGGG                CC^[GC]GG
BfaI         1169  CTAG                 C^TAG
BglI         1093  GCCCACTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          474  GAGACC               GGTCTC(1/5)
BsaWI        1509  ACCGGT               [AT]^CCGG[AT]
BspLU11I      116  ACATGT               A^CATGT
BspMI        1353  GCAGGT               ACCTGC(4/8)
BsrBI        1312  CCGCTC               CCGCTC(-3/-3)
BsrFI        1509  ACCGGT               [GA]^CCGG[CT]
BstBI        1059  TTCGAA               TT^CGAA
BstEII        274  GGTTACC              G^GT[ACGT]ACC
BstPI         274  GGTTACC              G^GT[ACGT]ACC
BstXI         362  CCAATATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         768  GGATCC               [GA]^GATC[CT]
CfoI           43  GCGC                 GCG^C
Cfr10I       1509  ACCGGT               [GA]^CCGG[CT]
DraII          63  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI         1077  CCATGG               C^C[GA][CT]GG
EaeI          528  TGGCCA               [CT]^GGCC[GA]
EcoO109I       63  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       274  GGTTACC              G^GT[ACGT]ACC
EcoT22I      1162  ATGCAT               ATGCA^T
FbaI         1052  TGATCA               T^GATCA
HgaI           49  GACGC                GACGC(5/10)
HhaI           43  GCGC                 GCG^C
HinP1I         43  GCGC                 G^CGC
HincII       1021  GTTGAC               GT[CT]^[GA]AC
HindII       1021  GTTGAC               GT[CT]^[GA]AC
MaeI         1169  CTAG                 C^TAG
MflI          768  GGATCC               [GA]^GATC[CT]
MluNI         528  TGGCCA               TGG^CCA
MscI          528  TGGCCA               TGG^CCA
MseI          545  TTAA                 T^TAA
NciI         1135  CCGGG                CC^[GC]GG
NcoI         1077  CCATGG               C^CATGG
NsiI         1162  ATGCAT               ATGCA^T
NspV         1059  TTCGAA               TT^CGAA
PinAI        1509  ACCGGT               A^CCGGT
PleI          268  GAGTC                GAGTC(4/5)
PpuMI          63  GGGACCT              [GA]G^G[AT]CC[CT]
SapI          910  GAAGAGC              GCTCTTC(1/4)
SfiI         1092  GGCCCACTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1059  TTCGAA               TT^CGAA
SphI         1547  GCATGC               GCATG^C
TaqI         1060  TCGA                 T^CGA
TfiI         1143  GAATC                G^A[AT]TC
Tru9I         545  TTAA                 T^TAA
TthHB8I      1060  TCGA                 T^CGA
XcmI          165  CCAGTCCTACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         768  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI          769  GATCC                GGATC(4/5)
              768  GGATC               
AspHI         250  GTGCAC               G[AT]GC[AT]^C
              563  GTGCTC              
BanII         778  GAGCCC               G[GA]GC[CT]^C
             1081  GGGCCC              
BbsI          196  GAAGAC               GAAGAC(2/6)
             1029  GAAGAC              
BpmI           23  CTCCAG               CTGGAG(16/14)
             1261  CTCCAG              
BpuAI         196  GAAGAC               GAAGAC(2/6)
             1029  GAAGAC              
BsgI         1445  CTGCAC               GTGCAG(16/14)
             1488  GTGCAG              
BsiHKAI       250  GTGCAC               G[AT]GC[AT]^C
              563  GTGCTC              
BsrDI         593  GCAATG               GCAATG(2/0)
             1465  GCAATG              
FauI           34  CCCGC                CCCGC(4/6)
             1272  CCCGC               
HapII        1135  CCGG                 C^CGG
             1510  CCGG                
HinfI         268  GAGTC                G^A[ACGT]TC
             1143  GAATC               
HpaII        1135  CCGG                 C^CGG
             1510  CCGG                
MaeII           5  ACGT                 A^CGT
             1025  ACGT                
MspI         1135  CCGG                 C^CGG
             1510  CCGG                
PflMI         240  CCACTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1461  CCAGGCAATGG         
PstI          491  CTGCAG               CTGCA^G
             1533  CTGCAG              
Van91I        240  CCACTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1461  CCAGGCAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      Tsp509I     TspRI       
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