Restriction map for pF1KA0808
 (Linear) (Six-base) MAPSORT of: KIAA0808.seq  Check: 5254  from: 1  to: 1578



KIAA0808.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1578 
   Size:        985     593
 
AflIII A'CryG_T

Cuts at:      0     116    1578 
   Size:        116    1462
 
AgeI A'CCGG_T

Cuts at:      0    1509    1578 
   Size:       1509      69
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     581     613    1578 
   Size:        581      32     965

  Fragments arranged by size: 

                 965     581      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1219    1578 
   Size:       1219     359
 
ApaI G_GGCC'C

Cuts at:      0    1085    1578 
   Size:       1085     493
 
ApaLI G'TGCA_C

Cuts at:      0     250    1578 
   Size:        250    1328
 
BamHI G'GATC_C

Cuts at:      0     768    1578 
   Size:        768     810
 
BanII G_rGCy'C

Cuts at:      0     782    1085    1578 
   Size:        782     303     493

  Fragments arranged by size: 

                 782     493     303
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     203    1036    1578 
   Size:        203     833     542

  Fragments arranged by size: 

                 833     542     203
 
BbvCI CC'TCA_GC

Cuts at:      0    1046    1578 
   Size:       1046     532
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1008    1578 
   Size:       1008     570
 
BclI T'GATC_A

Cuts at:      0    1052    1578 
   Size:       1052     526
 
BfrBI ATG'CAT

Cuts at:      0    1164    1578 
   Size:       1164     414
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1099    1578 
   Size:       1099     479
 
Bme1580I G_kGCm'C

Cuts at:      0     254    1085    1578 
   Size:        254     831     493

  Fragments arranged by size: 

                 831     493     254
 
BmgBI CAC'GTC

Cuts at:      0    1026    1578 
   Size:       1026     552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       8    1246    1578 
   Size:          8    1238     332

  Fragments arranged by size: 

                1238     332       8
 
Bpu10I CC'TnA_GC

Cuts at:      0    1046    1578 
   Size:       1046     532
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     468    1578 
   Size:        468    1110
 
BsaWI w'CCGG_w

Cuts at:      0    1509    1578 
   Size:       1509      69
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     212    1034    1241    1244    1578 
   Size:        212     822     207       3     334

  Fragments arranged by size: 

                 822     334     212     207       3
 
BseYI C'CCAG_C

Cuts at:      0      29     369    1084    1578 
   Size:         29     340     715     494

  Fragments arranged by size: 

                 715     494     340      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1430    1509    1578 
   Size:       1430      79      69

  Fragments arranged by size: 

                1430      79      69
 
BsiHKAI G_wGCw'C

Cuts at:      0     254     567    1578 
   Size:        254     313    1011

  Fragments arranged by size: 

                1011     313     254
 
Bsp1286I G_dGCh'C

Cuts at:      0     254     567     782    1085    1578 
   Size:        254     313     215     303     493

  Fragments arranged by size: 

                 493     313     303     254     215
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1344    1578 
   Size:       1344     234
 
BsrBI CCG'CTC

Cuts at:      0    1314    1578 
   Size:       1314     264
 
BsrDI GCAATG_nn'

Cuts at:      0     600    1472    1578 
   Size:        600     872     106

  Fragments arranged by size: 

                 872     600     106
 
BsrFI r'CCGG_y

Cuts at:      0    1509    1578 
   Size:       1509      69
 
BstBI TT'CG_AA

Cuts at:      0    1060    1578 
   Size:       1060     518
 
BstEII G'GTnAC_C

Cuts at:      0     274    1578 
   Size:        274    1304
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     369    1578 
   Size:        369    1209
 
BstYI r'GATC_y

Cuts at:      0     768    1578 
   Size:        768     810
 
BtgI C'CryG_G

Cuts at:      0    1077    1578 
   Size:       1077     501
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     692    1578 
   Size:        692     886
 
BtsI GCAGTG_nn'

Cuts at:      0     385    1578 
   Size:        385    1193
 
EaeI y'GGCC_r

Cuts at:      0     528    1578 
   Size:        528    1050
 
EarI CTCTTCn'nnn_

Cuts at:      0     905    1283    1406    1578 
   Size:        905     378     123     172

  Fragments arranged by size: 

                 905     378     172     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       8     985    1246    1578 
   Size:          8     977     261     332

  Fragments arranged by size: 

                 977     332     261       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64    1578 
   Size:         64    1514
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     670     702    1578 
   Size:        670      32     876

  Fragments arranged by size: 

                 876     670      32
 
HincII GTy'rAC

Cuts at:      0    1023    1578 
   Size:       1023     555
 
MscI TGG'CCA

Cuts at:      0     530    1578 
   Size:        530    1048
 
MslI CAynn'nnrTG

Cuts at:      0     121     257     406     668    1240    1578 
   Size:        121     136     149     262     572     338

  Fragments arranged by size: 

                 572     338     262     149     136     121
 
MspA1I CmG'CkG

Cuts at:      0      40     534    1050    1444    1578 
   Size:         40     494     516     394     134

  Fragments arranged by size: 

                 516     494     394     134      40
 
NcoI C'CATG_G

Cuts at:      0    1077    1578 
   Size:       1077     501
 
NsiI A_TGCA'T

Cuts at:      0    1166    1578 
   Size:       1166     412
 
NspI r_CATG'y

Cuts at:      0     120    1299    1551    1578 
   Size:        120    1179     252      27

  Fragments arranged by size: 

                1179     252     120      27
 
PciI A'CATG_T

Cuts at:      0     116    1578 
   Size:        116    1462
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     246    1467    1578 
   Size:        246    1221     111

  Fragments arranged by size: 

                1221     246     111
 
PpuMI rG'GwC_Cy

Cuts at:      0      64    1578 
   Size:         64    1514
 
PspOMI G'GGCC_C

Cuts at:      0    1081    1578 
   Size:       1081     497
 
PstI C_TGCA'G

Cuts at:      0     495    1537    1578 
   Size:        495    1042      41

  Fragments arranged by size: 

                1042     495      41
 
PvuII CAG'CTG

Cuts at:      0     534    1050    1444    1578 
   Size:        534     516     394     134

  Fragments arranged by size: 

                 534     516     394     134
 
SapI GCTCTTCn'nnn_

Cuts at:      0     905    1578 
   Size:        905     673
 
SfcI C'TryA_G

Cuts at:      0     491    1008    1226    1439    1533    1578 
   Size:        491     517     218     213      94      45

  Fragments arranged by size: 

                 517     491     218     213      94      45
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1099    1578 
   Size:       1099     479
 
SphI G_CATG'C

Cuts at:      0    1551    1578 
   Size:       1551      27
 
StyI C'CwwG_G

Cuts at:      0     478    1077    1332    1578 
   Size:        478     599     255     246

  Fragments arranged by size: 

                 599     478     255     246
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1392    1474    1578 
   Size:       1392      82     104

  Fragments arranged by size: 

                1392     104      82
 
TatI w'GTAC_w

Cuts at:      0     443    1578 
   Size:        443    1135
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     172    1578 
   Size:        172    1406
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AloI    AlwNI     ApaI    ApaLI    BamHI
    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI Bme1580I
    BmgBI     BpmI   Bpu10I     BsaI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI  Eco57MI
 EcoO109I    Hin4I   HincII     MscI     MslI   MspA1I     NcoI     NsiI
     NspI     PciI    PflMI    PpuMI   PspOMI     PstI    PvuII     SapI
     SfcI     SfiI     SphI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AleI     AlfI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI     BbeI     BcgI    BglII     BlpI     BmrI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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