Restriction map for pF1KA0744
 (Linear) (Six-base) MAPSORT of: KIAA0744.seq  Check: 7168  from: 1  to: 1770



KIAA0744.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

Acc65I G'GTAC_C

Cuts at:      0    1306    1770 
   Size:       1306     464
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1504    1510    1770 
   Size:        232    1272       6     260

  Fragments arranged by size: 

                1272     260     232       6
 
AlwNI CAG_nnn'CTG

Cuts at:      0     189     315     817    1770 
   Size:        189     126     502     953

  Fragments arranged by size: 

                 953     502     189     126
 
ApoI r'AATT_y

Cuts at:      0    1032    1286    1770 
   Size:       1032     254     484

  Fragments arranged by size: 

                1032     484     254
 
BanI G'GyrC_C

Cuts at:      0    1306    1770 
   Size:       1306     464
 
BanII G_rGCy'C

Cuts at:      0      59     665    1770 
   Size:         59     606    1105

  Fragments arranged by size: 

                1105     606      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1556    1770 
   Size:       1556     214
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1576    1610    1770 
   Size:       1576      34     160

  Fragments arranged by size: 

                1576     160      34
 
BclI T'GATC_A

Cuts at:      0      11    1770 
   Size:         11    1759
 
BlpI GC'TnA_GC

Cuts at:      0     242    1379    1770 
   Size:        242    1137     391

  Fragments arranged by size: 

                1137     391     242
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     818    1341    1624    1770 
   Size:        818     523     283     146

  Fragments arranged by size: 

                 818     523     283     146
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      78    1770 
   Size:         78    1692
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     337     794    1727    1770 
   Size:         73     264     457     933      43

  Fragments arranged by size: 

                 933     457     264      73      43
 
Bpu10I CC'TnA_GC

Cuts at:      0    1072    1770 
   Size:       1072     698
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1525    1770 
   Size:       1525     245
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     278     308    1770 
   Size:        278      30    1462

  Fragments arranged by size: 

                1462     278      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     365     723    1770 
   Size:        365     358    1047

  Fragments arranged by size: 

                1047     365     358
 
BseYI C'CCAG_C

Cuts at:      0    1016    1770 
   Size:       1016     754
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1337    1770 
   Size:       1337     433
 
BsiHKAI G_wGCw'C

Cuts at:      0     618    1372    1770 
   Size:        618     754     398

  Fragments arranged by size: 

                 754     618     398
 
BsmI GAATG_Cn'

Cuts at:      0     923    1770 
   Size:        923     847
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1054    1770 
   Size:       1054     716
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     618     665    1372    1770 
   Size:         59     559      47     707     398

  Fragments arranged by size: 

                 707     559     398      59      47
 
BspHI T'CATG_A

Cuts at:      0     933    1770 
   Size:        933     837
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     959    1770 
   Size:        959     811
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     622    1770 
   Size:        622    1148
 
BstBI TT'CG_AA

Cuts at:      0     645    1030    1465    1770 
   Size:        645     385     435     305

  Fragments arranged by size: 

                 645     435     385     305
 
BstYI r'GATC_y

Cuts at:      0    1442    1676    1770 
   Size:       1442     234      94

  Fragments arranged by size: 

                1442     234      94
 
Bsu36I CC'TnA_GG

Cuts at:      0      76      88    1770 
   Size:         76      12    1682

  Fragments arranged by size: 

                1682      76      12
 
BtgI C'CryG_G

Cuts at:      0     104    1770 
   Size:        104    1666
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     337    1555    1770 
   Size:        337    1218     215

  Fragments arranged by size: 

                1218     337     215
 
BtsI GCAGTG_nn'

Cuts at:      0     416    1013    1599    1770 
   Size:        416     597     586     171

  Fragments arranged by size: 

                 597     586     416     171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     118    1770 
   Size:        118    1652
 
EarI CTCTTCn'nnn_

Cuts at:      0    1514    1770 
   Size:       1514     256
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     747    1770 
   Size:        747    1023
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     232     337     794    1504    1510    1727    1770 
   Size:         73     159     105     457     710       6     217      43

  Fragments arranged by size: 

                 710     457     217     159     105      73      43       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     301     380     726    1770 
   Size:        301      79     346    1044

  Fragments arranged by size: 

                1044     346     301      79
 
EcoRI G'AATT_C

Cuts at:      0    1032    1770 
   Size:       1032     738
 
HaeII r_GCGC'y

Cuts at:      0    1561    1770 
   Size:       1561     209
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     498     530     547     579    1097    1129 
   Size:         16      32     450      32      17      32     518      32

Cuts at:   1129    1770 
   Size:        641

  Fragments arranged by size: 

                 641     518     450      32      32      32      32      17
                  16
 
HindIII A'AGCT_T

Cuts at:      0     436    1225    1770 
   Size:        436     789     545

  Fragments arranged by size: 

                 789     545     436
 
KpnI G_GTAC'C

Cuts at:      0    1310    1770 
   Size:       1310     460
 
MfeI C'AATT_G

Cuts at:      0     133    1770 
   Size:        133    1637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    1770 
   Size:        505    1265
 
MslI CAynn'nnrTG

Cuts at:      0    1136    1770 
   Size:       1136     634
 
MspA1I CmG'CkG

Cuts at:      0    1383    1770 
   Size:       1383     387
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     487     524     817     838    1686    1770 
   Size:        487      37     293      21     848      84

  Fragments arranged by size: 

                 848     487     293      84      37      21
 
PfoI T'CCnGG_A

Cuts at:      0    1679    1743    1770 
   Size:       1679      64      27

  Fragments arranged by size: 

                1679      64      27
 
PstI C_TGCA'G

Cuts at:      0    1190    1770 
   Size:       1190     580
 
PvuII CAG'CTG

Cuts at:      0    1383    1770 
   Size:       1383     387
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1514    1770 
   Size:       1514     256
 
SfcI C'TryA_G

Cuts at:      0    1186    1770 
   Size:       1186     584
 
SmlI C'TyrA_G

Cuts at:      0    1504    1770 
   Size:       1504     266
 
StyI C'CwwG_G

Cuts at:      0     992    1268    1770 
   Size:        992     276     502

  Fragments arranged by size: 

                 992     502     276
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1176    1770 
   Size:       1176     594
 
TatI w'GTAC_w

Cuts at:      0     418     599    1770 
   Size:        418     181    1171

  Fragments arranged by size: 

                1171     418     181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     990    1770 
   Size:        990     780
 
XmnI GAAnn'nnTTC

Cuts at:      0     149    1523    1770 
   Size:        149    1374     247

  Fragments arranged by size: 

                1374     247     149
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI    BanII     BbsI     BcgI
     BclI     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DrdI     EarI     EciI  Eco57MI    EcoNI    EcoRI    HaeII    Hin4I
  HindIII     KpnI     MfeI     MmeI     MslI   MspA1I    PflMI     PfoI
     PstI    PvuII     SapI     SfcI     SmlI     StyI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I     ClaI
    CspCI     DraI   DraIII     EaeI     EagI  EcoICRI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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