Restriction map for pF1KA0732
##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AclI        AflIII      AgeI        AhdI        
Alw44I      ApaLI       AscI        AseI        AsnI        
AspEI       BsaBI       BsiEI       BsiWI       BsmBI       
BspDI       BspLU11I    BsrFI       BssHII      BssSI       
BstBI       BstEII      BstPI       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
FseI        HpaI        MamI        MfeI        MluI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SbfI        ScaI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        XbaI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII       1815  TCCGGA               T^CCGGA
AflII        2968  CTTAAG               C^TTAAG
AviII        2369  TGCGCA               TGC^GCA
AvrII         364  CCTAGG               C^CTAGG
BbeI         2297  GGCGCC               GGCGC^C
BbrPI        4239  CACGTG               CAC^GTG
BbvCI         802  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1557  GTATCC               GTATCC(6/5)
BfrI         2968  CTTAAG               C^TTAAG
BlnI          364  CCTAGG               C^CTAGG
BsaI         2434  GAGACC               GGTCTC(1/5)
BseAI        1815  TCCGGA               T^CCGGA
Bsp1407I     3233  TGTACA               T^GTACA
BspEI        1815  TCCGGA               T^CCGGA
BspHI        2417  TCATGA               T^CATGA
BsrGI        3233  TGTACA               T^GTACA
Bst1107I     2331  GTATAC               GTA^TAC
BstAPI       2183  GCATGATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      2331  GTATAC               GTA^TAC
EcoRI         217  GAATTC               G^AATTC
EcoT22I      2181  ATGCAT               ATGCA^T
FspI         2369  TGCGCA               TGC^GCA
HindIII      2344  AAGCTT               A^AGCTT
KasI         2297  GGCGCC               G^GCGCC
KspI          702  CCGCGG               CCGC^GG
MroI         1815  TCCGGA               T^CCGGA
NarI         2297  GGCGCC               GG^CGCC
NsiI         2181  ATGCAT               ATGCA^T
PaeR7I       3940  CTCGAG               C^TCGAG
PflMI         610  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        4239  CACGTG               CAC^GTG
PmlI         4239  CACGTG               CAC^GTG
PshAI        3694  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2417  TCATGA               T^CATGA
SacII         702  CCGCGG               CCGC^GG
SalI         3174  GTCGAC               G^TCGAC
SapI         3424  GAAGAGC              GCTCTTC(1/4)
SexAI         509  ACCAGGT              A^CC[AT]GGT
SfiI           66  GGCCCCGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2297  GGCGCC               GGC^GCC
SphI         2813  GCATGC               GCATG^C
SspBI        3233  TGTACA               T^GTACA
Van91I        610  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         3940  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       2210  GGTACC               G^GTACC
             2261  GGTACC              
AcsI          217  GAATTC               [GA]^AATT[CT]
             2479  GAATTT              
AocI         3001  CCTGAGG              CC^T[ACGT]AGG
             3319  CCTCAGG             
ApaI         1596  GGGCCC               GGGCC^C
             4244  GGGCCC              
ApoI          217  GAATTC               [GA]^AATT[CT]
             2479  GAATTT              
Asp718I      2210  GGTACC               G^GTACC
             2261  GGTACC              
AspHI        2026  GAGCTC               G[AT]GC[AT]^C
             3718  GAGCTC              
AspI         1644  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2350  GACGCTGTC           
BamHI          50  GGATCC               G^GATCC
             1980  GGATCC              
BfaI          365  CTAG                 C^TAG
             2207  CTAG                
BsaAI        3334  TACGTG               [CT]AC^GT[GA]
             4239  CACGTG              
BsaWI        1815  TCCGGA               [AT]^CCGG[AT]
             4175  ACCGGA              
BsgI         1741  CTGCAC               GTGCAG(16/14)
             2955  CTGCAC              
BsiHKAI      2026  GAGCTC               G[AT]GC[AT]^C
             3718  GAGCTC              
BsrBI         735  CCGCTC               CCGCTC(-3/-3)
             2889  CCGCTC              
BsrDI        2280  CATTGC               GCAATG(2/0)
             3995  GCAATG              
Bsu36I       3001  CCTGAGG              CC^T[ACGT]AGG
             3319  CCTCAGG             
Eco81I       3001  CCTGAGG              CC^T[ACGT]AGG
             3319  CCTCAGG             
HincII       3174  GTCGAC               GT[CT]^[GA]AC
             4164  GTTGAC              
HindII       3174  GTCGAC               GT[CT]^[GA]AC
             4164  GTTGAC              
KpnI         2210  GGTACC               GGTAC^C
             2261  GGTACC              
MaeI          365  CTAG                 C^TAG
             2207  CTAG                
PflFI        1644  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2350  GACGCTGTC           
SacI         2026  GAGCTC               GAGCT^C
             3718  GAGCTC              
Tth111I      1644  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2350  GACGCTGTC           
XcmI          309  CCAAGAGCAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1106  CCAGGGATGATATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       Aor51HI     Asp700I     
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcgI        BclI        BcnI        
BglI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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