Restriction map for pF1KA0732
 (Circular) (Six-base) MAPSORT of: pF1KA0732.seq  Check: 5324  from: 1  to: 7370



pF1KA0732  7370 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3076    3076 
   Size:       7370
 
AccI GT'mk_AC

Cuts at:   2440    2629    3283    4408    2440 
   Size:        189     654    1125    5402

  Fragments arranged by size: 

                5402    1125     654     189
 
Acc65I G'GTAC_C

Cuts at:   2318    2369    4386    2318 
   Size:         51    2017    5302

  Fragments arranged by size: 

                5302    2017      51
 
AclI AA'CG_TT

Cuts at:   7210    7210 
   Size:       7370
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1003    2158    2395    3184    3520    3707    5195    5627    6374 
   Size:       1155     237     789     336     187    1488     432     747

Cuts at:   6374    6642    1003 
   Size:        268    1731

  Fragments arranged by size: 

                1731    1488    1155     789     747     432     336     268
                 237     187
 
AfeI AGC'GCT

Cuts at:    835     909    1981     835 
   Size:         74    1072    6224

  Fragments arranged by size: 

                6224    1072      74
 
AflII C'TTAA_G

Cuts at:   3076    3076 
   Size:       7370
 
AflIII A'CryG_T

Cuts at:   4722    5826    6803    4722 
   Size:       1104     977    5289

  Fragments arranged by size: 

                5289    1104     977
 
AgeI A'CCGG_T

Cuts at:   5758    5758 
   Size:       7370
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3495    3495 
   Size:       7370
 
AlwNI CAG_nnn'CTG

Cuts at:   1654    1848    1884    2034    2181    2854    3685    4675    6242 
   Size:        194      36     150     147     673     831     990    1567

Cuts at:   6242    1654 
   Size:       2782

  Fragments arranged by size: 

                2782    1567     990     831     673     194     150     147
                  36
 
ApaI G_GGCC'C

Cuts at:   1708    4356    1708 
   Size:       2648    4722
 
ApaLI G'TGCA_C

Cuts at:   6140    6140 
   Size:       7370
 
ApoI r'AATT_y

Cuts at:    325    2587    4374    6723    6979     325 
   Size:       2262    1787    2349     256     716

  Fragments arranged by size: 

                2349    2262    1787     716     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7370
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7370
 
AvaI C'yCGr_G

Cuts at:   1804    3399    4048    4390    1804 
   Size:       1595     649     342    4784

  Fragments arranged by size: 

                4784    1595     649     342
 
AvrII C'CTAG_G

Cuts at:    472     472 
   Size:       7370
 
BamHI G'GATC_C

Cuts at:    158    2088    4395     158 
   Size:       1930    2307    3133

  Fragments arranged by size: 

                3133    2307    1930
 
BanI G'GyrC_C

Cuts at:   1456    2318    2369    2405    3333    3576    3930    4386    4800 
   Size:        862      51      36     928     243     354     456     414

Cuts at:   4800    5048    5083    1456 
   Size:        248      35    3743

  Fragments arranged by size: 

                3743     928     862     456     414     354     248     243
                  51      36      35
 
BanII G_rGCy'C

Cuts at:    718     826    1708    2138    2602    3830    4089    4356    4384 
   Size:        108     882     430     464    1228     259     267      28

Cuts at:   4384    5414     718 
   Size:       1030    2674

  Fragments arranged by size: 

                2674    1228    1030     882     464     430     267     259
                 108      28
 
BbeI G_GCGC'C

Cuts at:   2409    4804    5052    2409 
   Size:       2395     248    4727

  Fragments arranged by size: 

                4727    2395     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   2216    2690    3137    3668    2216 
   Size:        474     447     531    5918

  Fragments arranged by size: 

                5918     531     474     447
 
BbvCI CC'TCA_GC

Cuts at:    911     911 
   Size:       7370
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3751    3785    4020    4054    4092    4126    3751 
   Size:         34     235      34      38      34    6995

  Fragments arranged by size: 

                6995     235      38      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1676    5268    6029    7370    1676 
   Size:       3592     761    1341    1676

  Fragments arranged by size: 

                3592    1676    1341     761
 
BclI T'GATC_A

Cuts at:   1491    2273    3944    4890    1491 
   Size:        782    1671     946    3971

  Fragments arranged by size: 

                3971    1671     946     782
 
BfrBI ATG'CAT

Cuts at:   2291    2291 
   Size:       7370
 
BglI GCCn_nnn'nGGC

Cuts at:    181    1454    3892    4248     181 
   Size:       1273    2438     356    3303

  Fragments arranged by size: 

                3303    2438    1273     356
 
BglII A'GATC_T

Cuts at:    249     539    2669    4885    6922     249 
   Size:        290    2130    2216    2037     697

  Fragments arranged by size: 

                2216    2130    2037     697     290
 
BlpI GC'TnA_GC

Cuts at:    888    1891    3095    3758    4482     888 
   Size:       1003    1204     663     724    3776

  Fragments arranged by size: 

                3776    1204    1003     724     663
 
Bme1580I G_kGCm'C

Cuts at:   1708    2283    2710    3935    4356    4995    5088    6144    1708 
   Size:        575     427    1225     421     639      93    1056    2934

  Fragments arranged by size: 

                2934    1225    1056     639     575     427     421      93
            
 
BmrI ACTGGGnnnn_n'

Cuts at:   1656    1693    2756    3173    4582    4663    4998    7142    1656 
   Size:         37    1063     417    1409      81     335    2144    1884

  Fragments arranged by size: 

                2144    1884    1409    1063     417     335      81      37
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3015    3471    5737    3015 
   Size:        456    2266    4648

  Fragments arranged by size: 

                4648    2266     456
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    142    1364    1890    1954    2686    3817    3925     142 
   Size:       1222     526      64     732    1131     108    3587

  Fragments arranged by size: 

                3587    1222    1131     732     526     108      64
 
Bpu10I CC'TnA_GC

Cuts at:    283     911    1589    2596    4211     283 
   Size:        628     678    1007    1615    3442

  Fragments arranged by size: 

                3442    1615    1007     678     628
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1272    2851    4546    5425    5917    6215    1272 
   Size:       1579    1695     879     492     298    2427

  Fragments arranged by size: 

                2427    1695    1579     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2536    7074    2536 
   Size:       4538    2832
 
BsaAI yAC'GTr

Cuts at:   3444    4349    4723    3444 
   Size:        905     374    6091

  Fragments arranged by size: 

                6091     905     374
 
BsaBI GATnn'nnATC

Cuts at:      6    4884    6921       6 
   Size:       4878    2037     455

  Fragments arranged by size: 

                4878    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    243    1519    2406    4801    5049    5751    7247     243 
   Size:       1276     887    2395     248     702    1496     366

  Fragments arranged by size: 

                2395    1496    1276     887     702     366     248
 
BsaWI w'CCGG_w

Cuts at:   1923    4283    4559    5080    5758    6032    6179    6590    1923 
   Size:       2360     276     521     678     274     147     411    2703

  Fragments arranged by size: 

                2703    2360     678     521     411     276     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1888    1918    1888 
   Size:         30    7340
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    613    1822    2460    3004    3224    3655    3658    4252     613 
   Size:       1209     638     544     220     431       3     594    3731

  Fragments arranged by size: 

                3731    1209     638     594     544     431     220       3
            
 
BseYI C'CCAG_C

Cuts at:   1523    2282    2381    2745    2966    3994    4152    4335    4795 
   Size:        759      99     364     221    1028     158     183     460

Cuts at:   4795    5553    6130    6626    1523 
   Size:        758     577     496    2267

  Fragments arranged by size: 

                2267    1028     759     758     577     496     460     364
                 221     183     158      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1834    3048    1834 
   Size:       1214    6156
 
BsiEI CG_ry'CG

Cuts at:    104    4912    4958    6166     104 
   Size:       4808      46    1208    1308

  Fragments arranged by size: 

                4808    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2138    3830    4384    5162    5352    6144    2138 
   Size:       1692     554     778     190     792    3364

  Fragments arranged by size: 

                3364    1692     792     778     554     190
 
BsmI GAATG_Cn'

Cuts at:   1328    2049    3547    1328 
   Size:        721    1498    5151

  Fragments arranged by size: 

                5151    1498     721
 
Bsp1286I G_dGCh'C

Cuts at:    718     826    1708    2138    2283    2602    2710    3830    3935 
   Size:        108     882     430     145     319     108    1120     105

Cuts at:   3935    4089    4356    4384    4995    5088    5162    5352    5414 
   Size:        154     267      28     611      93      74     190      62

Cuts at:   5414    6144     718 
   Size:        730    1944

  Fragments arranged by size: 

                1944    1120     882     730     611     430     319     267
                 190     154     145     108     108     105      93      74
                  62      28
 
BspEI T'CCGG_A

Cuts at:   1923    6590    1923 
   Size:       4667    2703
 
BspHI T'CATG_A

Cuts at:   2525    6546    2525 
   Size:       4021    3349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    542    1363    1509    1841    1907    2175    3076    4298    4420 
   Size:        821     146     332      66     268     901    1222     122

Cuts at:   4420    4634    4936    5317     542 
   Size:        214     302     381    2595

  Fragments arranged by size: 

                2595    1222     901     821     381     332     302     268
                 214     146     122      66
 
BsrBI CCG'CTC

Cuts at:     50     845    2999    5662    5716    7200    7365      50 
   Size:        795    2154    2663      54    1484     165      55

  Fragments arranged by size: 

                2663    2154    1484     795     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2387    4110    5282    2387 
   Size:       1723    1172    4475

  Fragments arranged by size: 

                4475    1723    1172
 
BsrFI r'CCGG_y

Cuts at:   5368    5549    5758    5368 
   Size:        181     209    6980

  Fragments arranged by size: 

                6980     209     181
 
BsrGI T'GTAC_A

Cuts at:   3341    3341 
   Size:       7370
 
BssSI C'ACGA_G

Cuts at:   5641    5999    5641 
   Size:        358    7012
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2297    4747    2297 
   Size:       2450    4920
 
BstBI TT'CG_AA

Cuts at:   5731    5731 
   Size:       7370
 
BstXI CCAn_nnnn'nTGG

Cuts at:    594    2664    2806    3246    3735    3994     594 
   Size:       2070     142     440     489     259    3970

  Fragments arranged by size: 

                3970    2070     489     440     259     142
 
BstYI r'GATC_y

Cuts at:      1     134     158     249     539    1417    1432    2030    2088 
   Size:        133      24      91     290     878      15     598      58

Cuts at:   2088    2669    3249    3782    4395    4862    4885    5220    5466 
   Size:        581     580     533     613     467      23     335     246

Cuts at:   5466    6478    6564    6576    6922       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     878     613     598     581     580     533     467
                 449     346     335     290     246     133      91      86
                  58      24      23      15      12
 
BstZ17I GTA'TAC

Cuts at:   2441    2441 
   Size:       7370
 
Bsu36I CC'TnA_GG

Cuts at:   3110    3428    3110 
   Size:        318    7052
 
BtgI C'CryG_G

Cuts at:    107     810    1107    1199    1449    2692    2805    3593    4098 
   Size:        703     297      92     250    1243     113     788     505

Cuts at:   4098     107 
   Size:       3379

  Fragments arranged by size: 

                3379    1243     788     703     505     297     250     113
                  92
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2301    4104    5320    5501    5664    2301 
   Size:       1803    1216     181     163    4007

  Fragments arranged by size: 

                4007    1803    1216     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1263    2441    2855    3323    4137    4613    1263 
   Size:       1178     414     468     814     476    4020

  Fragments arranged by size: 

                4020    1178     814     476     468     414
 
DraI TTT'AAA

Cuts at:   4369    4369 
   Size:       7370
 
DrdI GACnn_nn'nnGTC

Cuts at:   5076    5934    5076 
   Size:        858    6512
 
EaeI y'GGCC_r

Cuts at:   1104    3035    3743    3884    3965    4146    4955    5129    5520 
   Size:       1931     708     141      81     181     809     174     391

Cuts at:   5520    5547    6892    1104 
   Size:         27    1345    1582

  Fragments arranged by size: 

                1931    1582    1345     809     708     391     181     174
                 141      81      27
 
EagI C'GGCC_G

Cuts at:   4955    4955 
   Size:       7370
 
EarI CTCTTCn'nnn_

Cuts at:   1072    1160    2534    3377    3527    5393    5603    1072 
   Size:         88    1374     843     150    1866     210    2839

  Fragments arranged by size: 

                2839    1866    1374     843     210     150      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127     573    2628    3046    3075    5578    5888    6034    7180 
   Size:        446    2055     418      29    2503     310     146    1146

Cuts at:   7180     127 
   Size:        317

  Fragments arranged by size: 

                2503    2055    1146     446     418     317     310     146
                  29
 
EcoICRI GAG'CTC

Cuts at:   2136    3828    4382    2136 
   Size:       1692     554    5124

  Fragments arranged by size: 

                5124    1692     554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    142    1003    1364    1890    1954    2158    2395    2686    3184 
   Size:        861     361     526      64     204     237     291     498

Cuts at:   3184    3520    3707    3817    3925    5195    5627    6374    6642 
   Size:        336     187     110     108    1270     432     747     268

Cuts at:   6642     142 
   Size:        870

  Fragments arranged by size: 

                1270     870     861     747     526     498     432     361
                 336     291     268     237     204     187     110     108
                  64
 
EcoNI CCTnn'n_nnAGG

Cuts at:    471     616    1244    3382    3967     471 
   Size:        145     628    2138     585    3874

  Fragments arranged by size: 

                3874    2138     628     585     145
 
EcoO109I rG'GnC_Cy

Cuts at:   1054    1399    1476    1651    1705    1729    1929    2862    3348 
   Size:        345      77     175      54      24     200     933     486

Cuts at:   3348    3432    4509    1054 
   Size:         84    1077    3915

  Fragments arranged by size: 

                3915    1077     933     486     345     200     175      84
                  77      54      24
 
EcoRI G'AATT_C

Cuts at:    325    4374     325 
   Size:       4049    3321
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   5389    5389 
   Size:       7370
 
FspI TGC'GCA

Cuts at:   2479    5151    2479 
   Size:       2672    4698
 
HaeII r_GCGC'y

Cuts at:    837     911    1983    2409    4306    4804    5052    6074    7053 
   Size:         74    1072     426    1897     498     248    1022     979

Cuts at:   7053     837 
   Size:       1154

  Fragments arranged by size: 

                1897    1154    1072    1022     979     498     426     248
                  74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2968    3000    3241    3265    3273    3297    5212    5242    5244 
   Size:         32     241      24       8      24    1915      30       2

Cuts at:   5244    5274    2968 
   Size:         30    5064

  Fragments arranged by size: 

                5064    1915     241      32      30      30      24      24
                   8       2
 
HincII GTy'rAC

Cuts at:   3284    4274    4409    3284 
   Size:        990     135    6245

  Fragments arranged by size: 

                6245     990     135
 
HindIII A'AGCT_T

Cuts at:   2452    2452 
   Size:       7370
 
KasI G'GCGC_C

Cuts at:   2405    4800    5048    2405 
   Size:       2395     248    4727

  Fragments arranged by size: 

                4727    2395     248
 
KpnI G_GTAC'C

Cuts at:   2322    2373    4390    2322 
   Size:         51    2017    5302

  Fragments arranged by size: 

                5302    2017      51
 
MluI A'CGCG_T

Cuts at:   6803    6803 
   Size:       7370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    125    2625    6041    6225     125 
   Size:       2500    3416     184    1270

  Fragments arranged by size: 

                3416    2500    1270     184
 
MscI TGG'CCA

Cuts at:   3037    3745    3886    3967    4148    5131    3037 
   Size:        708     141      81     181     983    5276

  Fragments arranged by size: 

                5276     983     708     181     141      81
 
MslI CAynn'nnrTG

Cuts at:   2804    2944    3139    3925    5359    5486    2804 
   Size:        140     195     786    1434     127    4688

  Fragments arranged by size: 

                4688    1434     786     195     140     127
 
MspA1I CmG'CkG

Cuts at:    812     892    2970    3062    3099    3475    3695    4152    4481 
   Size:         80    2078      92      37     376     220     457     329

Cuts at:   4481    4795    5155    6168    6413     812 
   Size:        314     360    1013     245    1769

  Fragments arranged by size: 

                2078    1769    1013     457     376     360     329     314
                 245     220      92      80      37
 
NaeI GCC'GGC

Cuts at:   5551    5551 
   Size:       7370
 
NarI GG'CG_CC

Cuts at:   2406    4801    5049    2406 
   Size:       2395     248    4727

  Fragments arranged by size: 

                4727    2395     248
 
NcoI C'CATG_G

Cuts at:    107    1199    2805    3593     107 
   Size:       1092    1606     788    3884

  Fragments arranged by size: 

                3884    1606    1092     788
 
NgoMIV G'CCGG_C

Cuts at:   5549    5549 
   Size:       7370
 
NsiI A_TGCA'T

Cuts at:   2293    2293 
   Size:       7370
 
NspI r_CATG'y

Cuts at:   2644    2925    3265    4423    4923    5830    2644 
   Size:        281     340    1158     500     907    4184

  Fragments arranged by size: 

                4184    1158     907     500     340     281
 
PasI CC'CwG_GG

Cuts at:    313    1726    2703     313 
   Size:       1413     977    4980

  Fragments arranged by size: 

                4980    1413     977
 
PciI A'CATG_T

Cuts at:   5826    5826 
   Size:       7370
 
PflMI CCAn_nnn'nTGG

Cuts at:    724     724 
   Size:       7370
 
PfoI T'CCnGG_A

Cuts at:   3008    3155    3377    3794    3008 
   Size:        147     222     417    6584

  Fragments arranged by size: 

                6584     417     222     147
 
PmeI GTTT'AAAC

Cuts at:   4369    4369 
   Size:       7370
 
PmlI CAC'GTG

Cuts at:   4349    4349 
   Size:       7370
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1874    1906    6509    6541    1874 
   Size:         32    4603      32    2703

  Fragments arranged by size: 

                4603    2703      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1054    1399    1476    2862    3348    3432    1054 
   Size:        345      77    1386     486      84    4992

  Fragments arranged by size: 

                4992    1386     486     345      84      77
 
PshAI GACnn'nnGTC

Cuts at:   3806    3806 
   Size:       7370
 
PspOMI G'GGCC_C

Cuts at:   1704    4352    1704 
   Size:       2648    4722
 
PspXI vC'TCGA_Gb

Cuts at:   4048    4048 
   Size:       7370
 
PstI C_TGCA'G

Cuts at:    539    1838    1889    1970    2186    2850    2891    2930    3318 
   Size:       1299      51      81     216     664      41      39     388

Cuts at:   3318    4417    5102     539 
   Size:       1099     685    2807

  Fragments arranged by size: 

                2807    1299    1099     685     664     388     216      81
                  51      41      39
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7370
 
PvuII CAG'CTG

Cuts at:    892    2970    3099    3475    4152    4795    5155     892 
   Size:       2078     129     376     677     643     360    3107

  Fragments arranged by size: 

                3107    2078     677     643     376     360     129
 
RsrII CG'GwC_CG

Cuts at:   5565    5565 
   Size:       7370
 
SacI G_AGCT'C

Cuts at:   2138    3830    4384    2138 
   Size:       1692     554    5124

  Fragments arranged by size: 

                5124    1692     554
 
SacII CC_GC'GG

Cuts at:    813     813 
   Size:       7370
 
SalI G'TCGA_C

Cuts at:   3282    4407    3282 
   Size:       1125    6245
 
SanDI GG'GwC_CC

Cuts at:   1476    1476 
   Size:       7370
 
SapI GCTCTTCn'nnn_

Cuts at:   3527    5393    5603    3527 
   Size:       1866     210    5294

  Fragments arranged by size: 

                5294    1866     210
 
SbfI CC_TGCA'GG

Cuts at:   4417    4417 
   Size:       7370
 
ScaI AGT'ACT

Cuts at:   6887    6887 
   Size:       7370
 
SexAI A'CCwGG_T

Cuts at:    617     617 
   Size:       7370
 
SfcI C'TryA_G

Cuts at:     33     434     535    1690    1834    1885    1966    2182    2846 
   Size:        401     101    1155     144      51      81     216     664

Cuts at:   2846    2887    2926    3314    4413    5098    6091    6282      33 
   Size:         41      39     388    1099     685     993     191    1121

  Fragments arranged by size: 

                1155    1121    1099     993     685     664     401     388
                 216     191     144     101      81      51      41      39
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    181     181 
   Size:       7370
 
SfoI GGC'GCC

Cuts at:   2407    4802    5050    2407 
   Size:       2395     248    4727

  Fragments arranged by size: 

                4727    2395     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7370
 
SmaI CCC'GGG

Cuts at:   4392    4392 
   Size:       7370
 
SmlI C'TyrA_G

Cuts at:   1251    2866    3076    4048    4525    5440    5932    6194    1251 
   Size:       1615     210     972     477     915     492     262    2427

  Fragments arranged by size: 

                2427    1615     972     915     492     477     262     210
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7370
 
SphI G_CATG'C

Cuts at:   2925    4423    4923    2925 
   Size:       1498     500    5372

  Fragments arranged by size: 

                5372    1498     500
 
StyI C'CwwG_G

Cuts at:    107     472     965    1199    2301    2513    2805    3593    3968 
   Size:        365     493     234    1102     212     292     788     375

Cuts at:   3968    4504    4858     107 
   Size:        536     354    2619

  Fragments arranged by size: 

                2619    1102     788     536     493     375     365     354
                 292     234     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    365    4953    5086    5545    5754     365 
   Size:       4588     133     459     209    1981

  Fragments arranged by size: 

                4588    1981     459     209     133
 
TatI w'GTAC_w

Cuts at:   2955    3341    3917    6885    2955 
   Size:        386     576    2968    3440

  Fragments arranged by size: 

                3440    2968     576     386
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    634     666    1754    1785    1786    1817    3213    3245    3551 
   Size:         32    1088      31       1      31    1396      32     306

Cuts at:   3551    3583    4278    4310    6887    6919     634 
   Size:         32     695      32    2577      32    1085

  Fragments arranged by size: 

                2577    1396    1088    1085     695     306      32      32
                  32      32      32      31      31       1
 
Tth111I GACn'n_nGTC

Cuts at:   1755    2461    5167    1755 
   Size:        706    2706    3958

  Fragments arranged by size: 

                3958    2706     706
 
XbaI T'CTAG_A

Cuts at:   4401    4401 
   Size:       7370
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    424    1221    4865     424 
   Size:        797    3644    2929

  Fragments arranged by size: 

                3644    2929     797
 
XhoI C'TCGA_G

Cuts at:   4048    4048 
   Size:       7370
 
XmaI C'CCGG_G

Cuts at:   4390    4390 
   Size:       7370
 
XmnI GAAnn'nnTTC

Cuts at:    467    2047    2114     467 
   Size:       1580      67    5723

  Fragments arranged by size: 

                5723    1580      67
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AhdI     AleI     AloI     AscI     BaeI    BmgBI     BmtI
    BsiWI    BsmBI   BssHII   BstEII     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI     NruI
     PacI     PsiI     PsrI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     ZraI
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