Restriction map for pF1KA0732
 (Linear) (Six-base) MAPSORT of: KIAA0732.seq  Check: 2489  from: 1  to: 4257



KIAA0732.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2968    4257 
   Size:       2968    1289
 
AccI GT'mk_AC

Cuts at:      0    2332    2521    3175    4257 
   Size:       2332     189     654    1082

  Fragments arranged by size: 

                2332    1082     654     189
 
Acc65I G'GTAC_C

Cuts at:      0    2210    2261    4257 
   Size:       2210      51    1996

  Fragments arranged by size: 

                2210    1996      51
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     895    2050    2287    3076    3412    3599    4257 
   Size:        895    1155     237     789     336     187     658

  Fragments arranged by size: 

                1155     895     789     658     336     237     187
 
AfeI AGC'GCT

Cuts at:      0     727     801    1873    4257 
   Size:        727      74    1072    2384

  Fragments arranged by size: 

                2384    1072     727      74
 
AflII C'TTAA_G

Cuts at:      0    2968    4257 
   Size:       2968    1289
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3387    4257 
   Size:       3387     870
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1546    1740    1776    1926    2073    2746    3577    4257 
   Size:       1546     194      36     150     147     673     831     680

  Fragments arranged by size: 

                1546     831     680     673     194     150     147      36
            
 
ApaI G_GGCC'C

Cuts at:      0    1600    4248    4257 
   Size:       1600    2648       9

  Fragments arranged by size: 

                2648    1600       9
 
ApoI r'AATT_y

Cuts at:      0     217    2479    4257 
   Size:        217    2262    1778

  Fragments arranged by size: 

                2262    1778     217
 
AvaI C'yCGr_G

Cuts at:      0    1696    3291    3940    4257 
   Size:       1696    1595     649     317

  Fragments arranged by size: 

                1696    1595     649     317
 
AvrII C'CTAG_G

Cuts at:      0     364    4257 
   Size:        364    3893
 
BamHI G'GATC_C

Cuts at:      0      50    1980    4257 
   Size:         50    1930    2277

  Fragments arranged by size: 

                2277    1930      50
 
BanI G'GyrC_C

Cuts at:      0    1348    2210    2261    2297    3225    3468    3822    4257 
   Size:       1348     862      51      36     928     243     354     435

  Fragments arranged by size: 

                1348     928     862     435     354     243      51      36
            
 
BanII G_rGCy'C

Cuts at:      0     610     718    1600    2030    2494    3722    3981    4248 
   Size:        610     108     882     430     464    1228     259     267

Cuts at:   4248    4257 
   Size:          9

  Fragments arranged by size: 

                1228     882     610     464     430     267     259     108
                   9
 
BbeI G_GCGC'C

Cuts at:      0    2301    4257 
   Size:       2301    1956
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2108    2582    3029    3560    4257 
   Size:       2108     474     447     531     697

  Fragments arranged by size: 

                2108     697     531     474     447
 
BbvCI CC'TCA_GC

Cuts at:      0     803    4257 
   Size:        803    3454
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3643    3677    3912    3946    3984    4018    4257 
   Size:       3643      34     235      34      38      34     239

  Fragments arranged by size: 

                3643     239     235      38      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1568    4257 
   Size:       1568    2689
 
BclI T'GATC_A

Cuts at:      0    1383    2165    3836    4257 
   Size:       1383     782    1671     421

  Fragments arranged by size: 

                1671    1383     782     421
 
BfrBI ATG'CAT

Cuts at:      0    2183    4257 
   Size:       2183    2074
 
BglI GCCn_nnn'nGGC

Cuts at:      0      73    1346    3784    4140    4257 
   Size:         73    1273    2438     356     117

  Fragments arranged by size: 

                2438    1273     356     117      73
 
BglII A'GATC_T

Cuts at:      0     141     431    2561    4257 
   Size:        141     290    2130    1696

  Fragments arranged by size: 

                2130    1696     290     141
 
BlpI GC'TnA_GC

Cuts at:      0     780    1783    2987    3650    4257 
   Size:        780    1003    1204     663     607

  Fragments arranged by size: 

                1204    1003     780     663     607
 
Bme1580I G_kGCm'C

Cuts at:      0    1600    2175    2602    3827    4248    4257 
   Size:       1600     575     427    1225     421       9

  Fragments arranged by size: 

                1600    1225     575     427     421       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1548    1585    2648    3065    4257 
   Size:       1548      37    1063     417    1192

  Fragments arranged by size: 

                1548    1192    1063     417      37
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2907    3363    4257 
   Size:       2907     456     894

  Fragments arranged by size: 

                2907     894     456
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34    1256    1782    1846    2578    3709    3817    4257 
   Size:         34    1222     526      64     732    1131     108     440

  Fragments arranged by size: 

                1222    1131     732     526     440     108      64      34
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     175     803    1481    2488    4103    4257 
   Size:        175     628     678    1007    1615     154

  Fragments arranged by size: 

                1615    1007     678     628     175     154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1164    2743    4257 
   Size:       1164    1579    1514

  Fragments arranged by size: 

                1579    1514    1164
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2428    4257 
   Size:       2428    1829
 
BsaAI yAC'GTr

Cuts at:      0    3336    4241    4257 
   Size:       3336     905      16

  Fragments arranged by size: 

                3336     905      16
 
BsaHI Gr'CG_yC

Cuts at:      0     135    1411    2298    4257 
   Size:        135    1276     887    1959

  Fragments arranged by size: 

                1959    1276     887     135
 
BsaWI w'CCGG_w

Cuts at:      0    1815    4175    4257 
   Size:       1815    2360      82

  Fragments arranged by size: 

                2360    1815      82
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1780    1810    4257 
   Size:       1780      30    2447

  Fragments arranged by size: 

                2447    1780      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     505    1714    2352    2896    3116    3547    3550    4144 
   Size:        505    1209     638     544     220     431       3     594

Cuts at:   4144    4257 
   Size:        113

  Fragments arranged by size: 

                1209     638     594     544     505     431     220     113
                   3
 
BseYI C'CCAG_C

Cuts at:      0    1415    2174    2273    2637    2858    3886    4044    4227 
   Size:       1415     759      99     364     221    1028     158     183

Cuts at:   4227    4257 
   Size:         30

  Fragments arranged by size: 

                1415    1028     759     364     221     183     158      99
                  30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1726    2940    4257 
   Size:       1726    1214    1317

  Fragments arranged by size: 

                1726    1317    1214
 
BsiHKAI G_wGCw'C

Cuts at:      0    2030    3722    4257 
   Size:       2030    1692     535

  Fragments arranged by size: 

                2030    1692     535
 
BsmI GAATG_Cn'

Cuts at:      0    1220    1941    3439    4257 
   Size:       1220     721    1498     818

  Fragments arranged by size: 

                1498    1220     818     721
 
Bsp1286I G_dGCh'C

Cuts at:      0     610     718    1600    2030    2175    2494    2602    3722 
   Size:        610     108     882     430     145     319     108    1120

Cuts at:   3722    3827    3981    4248    4257 
   Size:        105     154     267       9

  Fragments arranged by size: 

                1120     882     610     430     319     267     154     145
                 108     108     105       9
 
BspEI T'CCGG_A

Cuts at:      0    1815    4257 
   Size:       1815    2442
 
BspHI T'CATG_A

Cuts at:      0    2417    4257 
   Size:       2417    1840
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     434    1255    1401    1733    1799    2067    2968    4190 
   Size:        434     821     146     332      66     268     901    1222

Cuts at:   4190    4257 
   Size:         67

  Fragments arranged by size: 

                1222     901     821     434     332     268     146      67
                  66
 
BsrBI CCG'CTC

Cuts at:      0     737    2891    4257 
   Size:        737    2154    1366

  Fragments arranged by size: 

                2154    1366     737
 
BsrDI GCAATG_nn'

Cuts at:      0    2279    4002    4257 
   Size:       2279    1723     255

  Fragments arranged by size: 

                2279    1723     255
 
BsrGI T'GTAC_A

Cuts at:      0    3233    4257 
   Size:       3233    1024
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2189    4257 
   Size:       2189    2068
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     486    2556    2698    3138    3627    3886    4257 
   Size:        486    2070     142     440     489     259     371

  Fragments arranged by size: 

                2070     489     486     440     371     259     142
 
BstYI r'GATC_y

Cuts at:      0      26      50     141     431    1309    1324    1922    1980 
   Size:         26      24      91     290     878      15     598      58

Cuts at:   1980    2561    3141    3674    4257 
   Size:        581     580     533     583

  Fragments arranged by size: 

                 878     598     583     581     580     533     290      91
                  58      26      24      15
 
BstZ17I GTA'TAC

Cuts at:      0    2333    4257 
   Size:       2333    1924
 
Bsu36I CC'TnA_GG

Cuts at:      0    3002    3320    4257 
   Size:       3002     318     937

  Fragments arranged by size: 

                3002     937     318
 
BtgI C'CryG_G

Cuts at:      0     702     999    1091    1341    2584    2697    3485    3990 
   Size:        702     297      92     250    1243     113     788     505

Cuts at:   3990    4257 
   Size:        267

  Fragments arranged by size: 

                1243     788     702     505     297     267     250     113
                  92
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2193    3996    4257 
   Size:       2193    1803     261

  Fragments arranged by size: 

                2193    1803     261
 
BtsI GCAGTG_nn'

Cuts at:      0    1155    2333    2747    3215    4029    4257 
   Size:       1155    1178     414     468     814     228

  Fragments arranged by size: 

                1178    1155     814     468     414     228
 
EaeI y'GGCC_r

Cuts at:      0     996    2927    3635    3776    3857    4038    4257 
   Size:        996    1931     708     141      81     181     219

  Fragments arranged by size: 

                1931     996     708     219     181     141      81
 
EarI CTCTTCn'nnn_

Cuts at:      0     964    1052    2426    3269    3419    4257 
   Size:        964      88    1374     843     150     838

  Fragments arranged by size: 

                1374     964     843     838     150      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     465    2520    2938    2967    4257 
   Size:         19     446    2055     418      29    1290

  Fragments arranged by size: 

                2055    1290     446     418      29      19
 
EcoICRI GAG'CTC

Cuts at:      0    2028    3720    4257 
   Size:       2028    1692     537

  Fragments arranged by size: 

                2028    1692     537
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     895    1256    1782    1846    2050    2287    2578 
   Size:         34     861     361     526      64     204     237     291

Cuts at:   2578    3076    3412    3599    3709    3817    4257 
   Size:        498     336     187     110     108     440

  Fragments arranged by size: 

                 861     526     498     440     361     336     291     237
                 204     187     110     108      64      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     363     508    1136    3274    3859    4257 
   Size:        363     145     628    2138     585     398

  Fragments arranged by size: 

                2138     628     585     398     363     145
 
EcoO109I rG'GnC_Cy

Cuts at:      0     946    1291    1368    1543    1597    1621    1821    2754 
   Size:        946     345      77     175      54      24     200     933

Cuts at:   2754    3240    3324    4257 
   Size:        486      84     933

  Fragments arranged by size: 

                 946     933     933     486     345     200     175      84
                  77      54      24
 
EcoRI G'AATT_C

Cuts at:      0     217    4257 
   Size:        217    4040
 
FspI TGC'GCA

Cuts at:      0    2371    4257 
   Size:       2371    1886
 
HaeII r_GCGC'y

Cuts at:      0     729     803    1875    2301    4198    4257 
   Size:        729      74    1072     426    1897      59

  Fragments arranged by size: 

                1897    1072     729     426      74      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2860    2892    3133    3157    3165    3189    4257 
   Size:       2860      32     241      24       8      24    1068

  Fragments arranged by size: 

                2860    1068     241      32      24      24       8
 
HincII GTy'rAC

Cuts at:      0    3176    4166    4257 
   Size:       3176     990      91

  Fragments arranged by size: 

                3176     990      91
 
HindIII A'AGCT_T

Cuts at:      0    2344    4257 
   Size:       2344    1913
 
KasI G'GCGC_C

Cuts at:      0    2297    4257 
   Size:       2297    1960
 
KpnI G_GTAC'C

Cuts at:      0    2214    2265    4257 
   Size:       2214      51    1992

  Fragments arranged by size: 

                2214    1992      51
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      17    2517    4257 
   Size:         17    2500    1740

  Fragments arranged by size: 

                2500    1740      17
 
MscI TGG'CCA

Cuts at:      0    2929    3637    3778    3859    4040    4257 
   Size:       2929     708     141      81     181     217

  Fragments arranged by size: 

                2929     708     217     181     141      81
 
MslI CAynn'nnrTG

Cuts at:      0    2696    2836    3031    3817    4257 
   Size:       2696     140     195     786     440

  Fragments arranged by size: 

                2696     786     440     195     140
 
MspA1I CmG'CkG

Cuts at:      0     704     784    2862    2954    2991    3367    3587    4044 
   Size:        704      80    2078      92      37     376     220     457

Cuts at:   4044    4257 
   Size:        213

  Fragments arranged by size: 

                2078     704     457     376     220     213      92      80
                  37
 
NarI GG'CG_CC

Cuts at:      0    2298    4257 
   Size:       2298    1959
 
NcoI C'CATG_G

Cuts at:      0    1091    2697    3485    4257 
   Size:       1091    1606     788     772

  Fragments arranged by size: 

                1606    1091     788     772
 
NsiI A_TGCA'T

Cuts at:      0    2185    4257 
   Size:       2185    2072
 
NspI r_CATG'y

Cuts at:      0    2536    2817    3157    4257 
   Size:       2536     281     340    1100

  Fragments arranged by size: 

                2536    1100     340     281
 
PasI CC'CwG_GG

Cuts at:      0     205    1618    2595    4257 
   Size:        205    1413     977    1662

  Fragments arranged by size: 

                1662    1413     977     205
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     616    4257 
   Size:        616    3641
 
PfoI T'CCnGG_A

Cuts at:      0    2900    3047    3269    3686    4257 
   Size:       2900     147     222     417     571

  Fragments arranged by size: 

                2900     571     417     222     147
 
PmlI CAC'GTG

Cuts at:      0    4241    4257 
   Size:       4241      16
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1766    1798    4257 
   Size:       1766      32    2459

  Fragments arranged by size: 

                2459    1766      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     946    1291    1368    2754    3240    3324    4257 
   Size:        946     345      77    1386     486      84     933

  Fragments arranged by size: 

                1386     946     933     486     345      84      77
 
PshAI GACnn'nnGTC

Cuts at:      0    3698    4257 
   Size:       3698     559
 
PspOMI G'GGCC_C

Cuts at:      0    1596    4244    4257 
   Size:       1596    2648      13

  Fragments arranged by size: 

                2648    1596      13
 
PspXI vC'TCGA_Gb

Cuts at:      0    3940    4257 
   Size:       3940     317
 
PstI C_TGCA'G

Cuts at:      0     431    1730    1781    1862    2078    2742    2783    2822 
   Size:        431    1299      51      81     216     664      41      39

Cuts at:   2822    3210    4257 
   Size:        388    1047

  Fragments arranged by size: 

                1299    1047     664     431     388     216      81      51
                  41      39
 
PvuII CAG'CTG

Cuts at:      0     784    2862    2991    3367    4044    4257 
   Size:        784    2078     129     376     677     213

  Fragments arranged by size: 

                2078     784     677     376     213     129
 
SacI G_AGCT'C

Cuts at:      0    2030    3722    4257 
   Size:       2030    1692     535

  Fragments arranged by size: 

                2030    1692     535
 
SacII CC_GC'GG

Cuts at:      0     705    4257 
   Size:        705    3552
 
SalI G'TCGA_C

Cuts at:      0    3174    4257 
   Size:       3174    1083
 
SanDI GG'GwC_CC

Cuts at:      0    1368    4257 
   Size:       1368    2889
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3419    4257 
   Size:       3419     838
 
SexAI A'CCwGG_T

Cuts at:      0     509    4257 
   Size:        509    3748
 
SfcI C'TryA_G

Cuts at:      0     326     427    1582    1726    1777    1858    2074    2738 
   Size:        326     101    1155     144      51      81     216     664

Cuts at:   2738    2779    2818    3206    4257 
   Size:         41      39     388    1051

  Fragments arranged by size: 

                1155    1051     664     388     326     216     144     101
                  81      51      41      39
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      73    4257 
   Size:         73    4184
 
SfoI GGC'GCC

Cuts at:      0    2299    4257 
   Size:       2299    1958
 
SmlI C'TyrA_G

Cuts at:      0    1143    2758    2968    3940    4257 
   Size:       1143    1615     210     972     317

  Fragments arranged by size: 

                1615    1143     972     317     210
 
SphI G_CATG'C

Cuts at:      0    2817    4257 
   Size:       2817    1440
 
StyI C'CwwG_G

Cuts at:      0     364     857    1091    2193    2405    2697    3485    3860 
   Size:        364     493     234    1102     212     292     788     375

Cuts at:   3860    4257 
   Size:        397

  Fragments arranged by size: 

                1102     788     493     397     375     364     292     234
                 212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     257    4257 
   Size:        257    4000
 
TatI w'GTAC_w

Cuts at:      0    2847    3233    3809    4257 
   Size:       2847     386     576     448

  Fragments arranged by size: 

                2847     576     448     386
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     526     558    1646    1677    1678    1709    3105    3137 
   Size:        526      32    1088      31       1      31    1396      32

Cuts at:   3137    3443    3475    4170    4202    4257 
   Size:        306      32     695      32      55

  Fragments arranged by size: 

                1396    1088     695     526     306      55      32      32
                  32      32      31      31       1
 
Tth111I GACn'n_nGTC

Cuts at:      0    1647    2353    4257 
   Size:       1647     706    1904

  Fragments arranged by size: 

                1904    1647     706
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     316    1113    4257 
   Size:        316     797    3144

  Fragments arranged by size: 

                3144     797     316
 
XhoI C'TCGA_G

Cuts at:      0    3940    4257 
   Size:       3940     317
 
XmnI GAAnn'nnTTC

Cuts at:      0     359    1939    2006    4257 
   Size:        359    1580      67    2251

  Fragments arranged by size: 

                2251    1580     359      67
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII     AlfI    AlwNI
     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NsiI     NspI     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SacI    SacII     SalI    SanDI     SapI    SexAI
     SfcI     SfiI     SfoI     SmlI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI   AflIII     AgeI     AhdI     AleI     AloI    ApaLI
     AscI     AseI    AsiSI     BaeI    BmgBI     BmtI    BsaBI    BsiEI
    BsiWI    BsmBI    BsrFI   BssHII    BssSI    BstBI   BstEII     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoRV     FalI     FseI
    FspAI     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PciI     PmeI     PsiI     PsrI     PvuI
    RsrII     SbfI     ScaI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     XbaI     XmaI     ZraI
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