Restriction map for pF1KA0687
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BbeI        BbrPI       
BbvCI       BciVI       BclI        BglI        BglII       
BlnI        BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspMI       BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraII       DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO109I    EcoO65I     EcoRI       FbaI        FseI        
FspI        Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NarI        NcoI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        RsrII       SacI        SacII       
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII        1217  CGCG                 CG^CG
AclI         1094  AACGTT               AA^CGTT
AflII         904  CTTAAG               C^TTAAG
AlwNI        1513  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI         1887  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BfrI          904  CTTAAG               C^TTAAG
BlpI          526  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      526  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2336  TACGTG               [CT]AC^GT[GA]
BsaBI        1481  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          489  GAATGC               GAATGC(1/-1)
Bsp1407I     2439  TGTACA               T^GTACA
BspHI        3077  TCATGA               T^CATGA
BsrFI        1842  GCCGGC               [GA]^CCGG[CT]
BsrGI        2439  TGTACA               T^GTACA
BstAPI        679  GCAGAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1217  CGCG                 CG^CG
BstXI         668  CCATTGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         526  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1842  GCCGGC               [GA]^CCGG[CT]
DraIII       2102  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        3083  CCTTAGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         457  GATATC               GAT^ATC
EcoT22I       706  ATGCAT               ATGCA^T
FauI           54  GCGGG                CCCGC(4/6)
HaeII        1514  AGCGCC               [GA]GCGC^[CT]
HgaI         1765  GCGTC                GACGC(5/10)
HindIII      1167  AAGCTT               A^AGCTT
MamI         1481  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1217  CGCG                 CG^CG
NaeI         1842  GCCGGC               GCC^GGC
NdeI         2831  CATATG               CA^TATG
NgoMIV       1842  GCCGGC               G^CCGGC
NsiI          706  ATGCAT               ATGCA^T
PflFI        1887  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     1094  AACGTT               AA^CGTT
PvuII         529  CAGCTG               CAG^CTG
RcaI         3077  TCATGA               T^CATGA
SalI          125  GTCGAC               G^TCGAC
SapI         1105  GCTCTTC              GCTCTTC(1/4)
SmaI         1429  CCCGGG               CCC^GGG
SspBI        2439  TGTACA               T^GTACA
Tth111I      1887  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1429  CCCGGG               C^CCGGG
XmaI         1429  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaI         1180  CCCGAG               C^[CT]CG[GA]G
             1429  CCCGGG              
BalI         1604  TGGCCA               TGG^CCA
             2229  TGGCCA              
BbsI          379  GAAGAC               GAAGAC(2/6)
             2261  GAAGAC              
BcgI            9  CGACTCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1868  GCACTCTAATCG        
BpuAI         379  GAAGAC               GAAGAC(2/6)
             2261  GAAGAC              
BsoBI        1180  CCCGAG               C^[CT]CG[GA]G
             1429  CCCGGG              
BspLU11I      129  ACATGT               A^CATGT
             2703  ACATGT              
CfoI         1216  GCGC                 GCG^C
             1515  GCGC                
HhaI         1216  GCGC                 GCG^C
             1515  GCGC                
HinP1I       1216  GCGC                 G^CGC
             1515  GCGC                
HincII        125  GTCGAC               GT[CT]^[GA]AC
              508  GTTGAC              
HindII        125  GTCGAC               GT[CT]^[GA]AC
              508  GTTGAC              
MluNI        1604  TGGCCA               TGG^CCA
             2229  TGGCCA              
MscI         1604  TGGCCA               TGG^CCA
             2229  TGGCCA              
MspA1I        529  CAGCTG               C[AC]G^C[GT]G
              826  CAGCGG              
PstI         1075  CTGCAG               CTGCA^G
             1279  CTGCAG              
XcmI          155  CCATCAAAGTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2903  CCAATCAGACAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AocI        ApoI        AspHI       
AvaII       BamHI       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       EaeI        
EarI        Eco81I      EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com