Restriction map for pF1KA0595
 (Circular) (Six-base) MAPSORT of: pF1KA0595.seq  Check: 5925  from: 1  to: 8105



pF1KA0595  8105 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    249    1452    2634    2746     249 
   Size:       1203    1182     112    5608

  Fragments arranged by size: 

                5608    1203    1182     112
 
AccI GT'mk_AC

Cuts at:   1567    1598    1838    3091    5143    1567 
   Size:         31     240    1253    2052    4529

  Fragments arranged by size: 

                4529    2052    1253     240      31
 
Acc65I G'GTAC_C

Cuts at:   2320    5121    2320 
   Size:       2801    5304
 
AclI AA'CG_TT

Cuts at:   7945    7945 
   Size:       8105
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    855    1081    2300    3389    3590    3916    3923    5930    6362 
   Size:        226    1219    1089     201     326       7    2007     432

Cuts at:   6362    6715    7377     855 
   Size:        353     662    1583

  Fragments arranged by size: 

                2007    1583    1219    1089     662     432     353     326
                 226     201       7
 
AflIII A'CryG_T

Cuts at:   3034    5457    7257    7538    3034 
   Size:       2423    1800     281    3601

  Fragments arranged by size: 

                3601    2423    1800     281
 
AgeI A'CCGG_T

Cuts at:   3752    3752 
   Size:       8105
 
AleI CACnn'nnGTG

Cuts at:   4835    4835 
   Size:       8105
 
AlwNI CAG_nnn'CTG

Cuts at:    264     526     997    1306    3289    5029    5410    6848     264 
   Size:        262     471     309    1983    1740     381    1438    1521

  Fragments arranged by size: 

                1983    1740    1521    1438     471     381     309     262
            
 
ApaI G_GGCC'C

Cuts at:    163     319     671    3050    3064    4123     163 
   Size:        156     352    2379      14    1059    4145

  Fragments arranged by size: 

                4145    2379    1059     352     156      14
 
ApaLI G'TGCA_C

Cuts at:   4821    6943    4821 
   Size:       2122    5983
 
ApoI r'AATT_y

Cuts at:   1707    5041    5109    7458    7714    1707 
   Size:       3334      68    2349     256    2098

  Fragments arranged by size: 

                3334    2349    2098     256      68
 
AscI GG'CGCG_CC

Cuts at:    115     115 
   Size:       8105
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       8105
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8105
 
AvaI C'yCGr_G

Cuts at:    150     581    1670    1682    4157    5125     150 
   Size:        431    1089      12    2475     968    3130

  Fragments arranged by size: 

                3130    2475    1089     968     431      12
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1568    1601    1568 
   Size:         33    8072
 
BamHI G'GATC_C

Cuts at:   5130    5130 
   Size:       8105
 
BanI G'GyrC_C

Cuts at:   1419    2320    2646    2934    2959    2997    5121    5535    5783 
   Size:        901     326     288      25      38    2124     414     248

Cuts at:   5783    5818    1419 
   Size:         35    3706

  Fragments arranged by size: 

                3706    2124     901     414     326     288     248      38
                  35      25
 
BanII G_rGCy'C

Cuts at:    163     189     319     592     671    1476    1683    3050    3064 
   Size:         26     130     273      79     805     207    1367      14

Cuts at:   3064    3249    3791    3985    4063    4123    5119    6149     163 
   Size:        185     542     194      78      60     996    1030    2119

  Fragments arranged by size: 

                2119    1367    1030     996     805     542     273     207
                 194     185     130      79      78      60      26      14
            
 
BbeI G_GCGC'C

Cuts at:   5539    5787    5539 
   Size:        248    7857
 
BbsI GAAGACnn'nnnn_

Cuts at:   2524    2803    4442    4571    4734    5018    2524 
   Size:        279    1639     129     163     284    5611

  Fragments arranged by size: 

                5611    1639     284     279     163     129
 
BbvCI CC'TCA_GC

Cuts at:   1543    4203    4293    4880    1543 
   Size:       2660      90     587    4768

  Fragments arranged by size: 

                4768    2660     587      90
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4313    4347    4313 
   Size:         34    8071
 
BciVI GTATCCnnnnn_n'

Cuts at:    928    6003    7059    8105     928 
   Size:       5075    1056    1046     928

  Fragments arranged by size: 

                5075    1056    1046     928
 
BclI T'GATC_A

Cuts at:   2000    4170    5625    2000 
   Size:       2170    1455    4480

  Fragments arranged by size: 

                4480    2170    1455
 
BfrBI ATG'CAT

Cuts at:    936    4145     936 
   Size:       3209    4896
 
BglI GCCn_nnn'nGGC

Cuts at:    112    2874    3781    4376     112 
   Size:       2762     907     595    3841

  Fragments arranged by size: 

                3841    2762     907     595
 
BglII A'GATC_T

Cuts at:    506    5620    7657     506 
   Size:       5114    2037     954

  Fragments arranged by size: 

                5114    2037     954
 
BlpI GC'TnA_GC

Cuts at:   1988    5217    1988 
   Size:       3229    4876
 
Bme1580I G_kGCm'C

Cuts at:    163     319     671    1316    1424    2717    2939    3000    3050 
   Size:        156     352     645     108    1293     222      61      50

Cuts at:   3050    3064    3169    4123    4825    5730    5823    6947     163 
   Size:         14     105     954     702     905      93    1124    1321

  Fragments arranged by size: 

                1321    1293    1124     954     905     702     645     352
                 222     156     108     105      93      61      50      14
            
 
BmgBI CAC'GTC

Cuts at:   2191    2191 
   Size:       8105
 
BmrI ACTGGGnnnn_n'

Cuts at:    646     665     673    1186    2002    2149    2444    2771    3007 
   Size:         19       8     513     816     147     295     327     236

Cuts at:   3007    5317    5398    5733    7877     646 
   Size:       2310      81     335    2144     874

  Fragments arranged by size: 

                2310    2144     874     816     513     335     327     295
                 236     147      81      19       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    609    1029    4077    6472     609 
   Size:        420    3048    2395    2242

  Fragments arranged by size: 

                3048    2395    2242     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    484     801    1165    1204    1309    1636    2129    3101    3155 
   Size:        317     364      39     105     327     493     972      54

Cuts at:   3155    3655    3782     484 
   Size:        500     127    4807

  Fragments arranged by size: 

                4807     972     500     493     364     327     317     127
                 105      54      39
 
Bpu10I CC'TnA_GC

Cuts at:   1543    2600    4203    4293    4880    1543 
   Size:       1057    1603      90     587    4768

  Fragments arranged by size: 

                4768    1603    1057     587      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1105    2572    4335    5281    6160    6874    7172    1105 
   Size:       1467    1763     946     879     714     298    2038

  Fragments arranged by size: 

                2038    1763    1467     946     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    748     777    1215    2670    3409    3418    7809     748 
   Size:         29     438    1455     739       9    4391    1044

  Fragments arranged by size: 

                4391    1455    1044     739     438      29       9
 
BsaAI yAC'GTr

Cuts at:   3556    5458    3556 
   Size:       1902    6203
 
BsaBI GATnn'nnATC

Cuts at:      6    4623    5619    7656       6 
   Size:       4617     996    2037     455

  Fragments arranged by size: 

                4617    2037     996     455
 
BsaHI Gr'CG_yC

Cuts at:   5536    5784    6486    7982    5536 
   Size:        248     702    1496    5659

  Fragments arranged by size: 

                5659    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    168    3752    4007    4412    5294    5815    6493    6904    7051 
   Size:       3584     255     405     882     521     678     411     147

Cuts at:   7051    7325     168 
   Size:        274     948

  Fragments arranged by size: 

                3584     948     882     678     521     411     405     274
                 255     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2216    2233    2246    2263    4263    4293    2216 
   Size:         17      13      17    2000      30    6028

  Fragments arranged by size: 

                6028    2000      30      17      17      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    289     364     582     868     871     967    1005    1378    2224 
   Size:         75     218     286       3      96      38     373     846

Cuts at:   2224    3203    3239    3400    3665    4831    4897    4995     289 
   Size:        979      36     161     265    1166      66      98    3399

  Fragments arranged by size: 

                3399    1166     979     846     373     286     265     218
                 161      98      96      75      66      38      36       3
            
 
BseYI C'CCAG_C

Cuts at:    312     378     806     827    2094    2228    2508    2578    3516 
   Size:         66     428      21    1267     134     280      70     938

Cuts at:   3516    4122    5022    5530    6288    6953    7361     312 
   Size:        606     900     508     758     665     408    1056

  Fragments arranged by size: 

                1267    1056     938     900     758     665     606     508
                 428     408     280     134      70      66      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    583    2216    2713     583 
   Size:       1633     497    5975

  Fragments arranged by size: 

                5975    1633     497
 
BsiEI CG_ry'CG

Cuts at:    104    5647    5693    6923     104 
   Size:       5543      46    1230    1286

  Fragments arranged by size: 

                5543    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1235    1476    3249    3490    4825    5119    5897    6087    6947 
   Size:        241    1773     241    1335     294     778     190     860

Cuts at:   6947    1235 
   Size:       2393

  Fragments arranged by size: 

                2393    1773    1335     860     778     294     241     241
                 190
 
BsmI GAATG_Cn'

Cuts at:    551     551 
   Size:       8105
 
BsmBI CGTCTCn'nnnn_

Cuts at:    125     125 
   Size:       8105
 
Bsp1286I G_dGCh'C

Cuts at:    163     189     319     592     671    1235    1316    1424    1476 
   Size:         26     130     273      79     564      81     108      52

Cuts at:   1476    1683    2717    2939    3000    3050    3064    3169    3249 
   Size:        207    1034     222      61      50      14     105      80

Cuts at:   3249    3490    3791    3985    4063    4123    4825    5119    5730 
   Size:        241     301     194      78      60     702     294     611

Cuts at:   5730    5823    5897    6087    6149    6947     163 
   Size:         93      74     190      62     798    1321

  Fragments arranged by size: 

                1321    1034     798     702     611     564     301     294
                 273     241     222     207     194     190     130     108
                 105      93      81      80      79      78      74      62
                  61      60      52      50      26      14
 
BspEI T'CCGG_A

Cuts at:    168    4007     168 
   Size:       3839    4266
 
BspHI T'CATG_A

Cuts at:   6537    6537 
   Size:       8105
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    249     443    1452    2634    2746    4325    5155    5369    5671 
   Size:        194    1009    1182     112    1579     830     214     302

Cuts at:   5671    6052     249 
   Size:        381    2302

  Fragments arranged by size: 

                2302    1579    1182    1009     830     381     302     214
                 194     112
 
BsrBI CCG'CTC

Cuts at:     50     248    4613    6397    6451    7935    8100      50 
   Size:        198    4365    1784      54    1484     165      55

  Fragments arranged by size: 

                4365    1784    1484     198     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   6017    6017 
   Size:       8105
 
BsrFI r'CCGG_y

Cuts at:   1413    2375    3752    6103    6284    1413 
   Size:        962    1377    2351     181    3234

  Fragments arranged by size: 

                3234    2351    1377     962     181
 
BssHII G'CGCG_C

Cuts at:    115     115 
   Size:       8105
 
BssSI C'ACGA_G

Cuts at:   2236    6376    7084    2236 
   Size:       4140     708    3257

  Fragments arranged by size: 

                4140    3257     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    264     606    4701    5482     264 
   Size:        342    4095     781    2887

  Fragments arranged by size: 

                4095    2887     781     342
 
BstBI TT'CG_AA

Cuts at:   6466    6466 
   Size:       8105
 
BstEII G'GTnAC_C

Cuts at:   1320    1320 
   Size:       8105
 
BstXI CCAn_nnnn'nTGG

Cuts at:    939    2472    3633     939 
   Size:       1533    1161    5411

  Fragments arranged by size: 

                5411    1533    1161
 
BstYI r'GATC_y

Cuts at:      1     506     717    1187    4197    5130    5597    5620    5955 
   Size:        505     211     470    3010     933     467      23     335

Cuts at:   5955    6201    6507    6519    6605    7657       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                3010    1052     933     505     470     467     449     335
                 306     246     211      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    950    1127    1619    4241    5092     950 
   Size:        177     492    2622     851    3963

  Fragments arranged by size: 

                3963    2622     851     492     177
 
BtgI C'CryG_G

Cuts at:    107     189    3240    3957     107 
   Size:         82    3051     717    4255

  Fragments arranged by size: 

                4255    3051     717      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1190    6055    6236    6399    1190 
   Size:       4865     181     163    2896

  Fragments arranged by size: 

                4865    2896     181     163
 
BtsI GCAGTG_nn'

Cuts at:    201     373     843     992    1120    1889    2048    2204    2991 
   Size:        172     470     149     128     769     159     156     787

Cuts at:   2991    3844    4191    5348     201 
   Size:        853     347    1157    2958

  Fragments arranged by size: 

                2958    1157     853     787     769     470     347     172
                 159     156     149     128
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1878    1913    2777    2812    3092    3127    1878 
   Size:         35     864      35     280      35    6856

  Fragments arranged by size: 

                6856     864     280      35      35      35
 
DraI TTT'AAA

Cuts at:   5104    5104 
   Size:       8105
 
DraIII CAC_nnn'GTG

Cuts at:   3315    3315 
   Size:       8105
 
DrdI GACnn_nn'nnGTC

Cuts at:   1748    4003    5811    7155    1748 
   Size:       2255    1808    1344    2698

  Fragments arranged by size: 

                2698    2255    1808    1344
 
EaeI y'GGCC_r

Cuts at:    863     876    3237    3773    3851    4761    4908    5690    5864 
   Size:         13    2361     536      78     910     147     782     174

Cuts at:   5864    6255    6282    7627     863 
   Size:        391      27    1345    1341

  Fragments arranged by size: 

                2361    1345    1341     910     782     536     391     174
                 147      78      27      13
 
EagI C'GGCC_G

Cuts at:   5690    5690 
   Size:       8105
 
EarI CTCTTCn'nnn_

Cuts at:    716     765     845    1489    2405    3733    4551    4557    4578 
   Size:         49      80     644     916    1328     818       6      21

Cuts at:   4578    6128    6338     716 
   Size:       1550     210    2483

  Fragments arranged by size: 

                2483    1550    1328     916     818     644     210      80
                  49      21       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4124    6313    7055    7201    7915    4124 
   Size:       2189     742     146     714    4314

  Fragments arranged by size: 

                4314    2189     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1474    3247    5117    1474 
   Size:       1773    1870    4462

  Fragments arranged by size: 

                4462    1870    1773
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    484     801     855    1081    1165    1204    1309    1636    2129 
   Size:        317      54     226      84      39     105     327     493

Cuts at:   2129    2300    3101    3155    3389    3590    3655    3782    3916 
   Size:        171     801      54     234     201      65     127     134

Cuts at:   3916    3923    5930    6362    6715    7377     484 
   Size:          7    2007     432     353     662    1212

  Fragments arranged by size: 

                2007    1212     801     662     493     432     353     327
                 317     234     226     201     171     134     127     105
                  84      65      54      54      39       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:    424     424 
   Size:       8105
 
EcoO109I rG'GnC_Cy

Cuts at:    159     160     336     667    1765    2575    3047    3060    3067 
   Size:          1     176     331    1098     810     472      13       7

Cuts at:   3067    3743    4016    4475    5244     159 
   Size:        676     273     459     769    3020

  Fragments arranged by size: 

                3020    1098     810     769     676     472     459     331
                 273     176      13       7       1
 
EcoRI G'AATT_C

Cuts at:   1707    5109    1707 
   Size:       3402    4703
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3465    3555    4562    6124    3465 
   Size:         90    1007    1562    5446

  Fragments arranged by size: 

                5446    1562    1007      90
 
FspI TGC'GCA

Cuts at:   5886    5886 
   Size:       8105
 
HaeII r_GCGC'y

Cuts at:    223     242    4083    5539    5787    7017    7788     223 
   Size:         19    3841    1456     248    1230     771     540

  Fragments arranged by size: 

                3841    1456    1230     771     540     248      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    122     154    1333    1365    2233    2265    3198    3230    3333 
   Size:         32    1179      32     868      32     933      32     103

Cuts at:   3333    3365    3379    3411    4058    4090    4984    5016    5947 
   Size:         32      14      32     647      32     894      32     931

Cuts at:   5947    5977    5979    6009     122 
   Size:         30       2      30    2218

  Fragments arranged by size: 

                2218    1179     933     931     894     868     647     103
                  32      32      32      32      32      32      32      32
                  30      30      14       2
 
HincII GTy'rAC

Cuts at:    657    1839    1869    1929    1944    2034    2121    2151    3723 
   Size:       1182      30      60      15      90      87      30    1572

Cuts at:   3723    5144     657 
   Size:       1421    3618

  Fragments arranged by size: 

                3618    1572    1421    1182      90      87      60      30
                  30      15
 
HindIII A'AGCT_T

Cuts at:    769     769 
   Size:       8105
 
KasI G'GCGC_C

Cuts at:   5535    5783    5535 
   Size:        248    7857
 
KpnI G_GTAC'C

Cuts at:   2324    5125    2324 
   Size:       2801    5304
 
MluI A'CGCG_T

Cuts at:   7538    7538 
   Size:       8105
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4433    5027    6864    7048    4433 
   Size:        594    1837     184    5490

  Fragments arranged by size: 

                5490    1837     594     184
 
MscI TGG'CCA

Cuts at:    865     878    3239    3775    3853    4910    5866     865 
   Size:         13    2361     536      78    1057     956    3104

  Fragments arranged by size: 

                3104    2361    1057     956     536      78      13
 
MslI CAynn'nnrTG

Cuts at:   4835    6094    6221    4835 
   Size:       1259     127    6719

  Fragments arranged by size: 

                6719    1259     127
 
MspA1I CmG'CkG

Cuts at:    191     997    1171    1272    1696    3175    3931    4126    4305 
   Size:        806     174     101     424    1479     756     195     179

Cuts at:   4305    4514    5216    5530    5890    6674    6919     191 
   Size:        209     702     314     360     784     245    1377

  Fragments arranged by size: 

                1479    1377     806     784     756     702     424     360
                 314     245     209     195     179     174     101
 
NaeI GCC'GGC

Cuts at:   6286    6286 
   Size:       8105
 
NarI GG'CG_CC

Cuts at:   5536    5784    5536 
   Size:        248    7857
 
NcoI C'CATG_G

Cuts at:    107    3240    3957     107 
   Size:       3133     717    4255

  Fragments arranged by size: 

                4255    3133     717
 
NgoMIV G'CCGG_C

Cuts at:   6284    6284 
   Size:       8105
 
NsiI A_TGCA'T

Cuts at:    938    4147     938 
   Size:       3209    4896
 
NspI r_CATG'y

Cuts at:   2772    3038    3079    3359    5158    5658    7261    2772 
   Size:        266      41     280    1799     500    1603    3616

  Fragments arranged by size: 

                3616    1799    1603     500     280     266      41
 
PasI CC'CwG_GG

Cuts at:   1397    1933    2211    3064    3434    1397 
   Size:        536     278     853     370    6068

  Fragments arranged by size: 

                6068     853     536     370     278
 
PciI A'CATG_T

Cuts at:   3034    7257    3034 
   Size:       4223    3882
 
PflMI CCAn_nnn'nTGG

Cuts at:    997     997 
   Size:       8105
 
PfoI T'CCnGG_A

Cuts at:    920    1215    1235     920 
   Size:        295      20    7790

  Fragments arranged by size: 

                7790     295      20
 
PmeI GTTT'AAAC

Cuts at:   5104    5104 
   Size:       8105
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    626     658    6551    6583     626 
   Size:         32    5893      32    2148

  Fragments arranged by size: 

                5893    2148      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    336    2575    4016    4475     336 
   Size:       2239    1441     459    3966

  Fragments arranged by size: 

                3966    2239    1441     459
 
PspOMI G'GGCC_C

Cuts at:    159     315     667    3046    3060    4119     159 
   Size:        156     352    2379      14    1059    4145

  Fragments arranged by size: 

                4145    2379    1059     352     156      14
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3549    3581    3549 
   Size:         32    8073
 
PstI C_TGCA'G

Cuts at:    365    1896    2043    2531    2757    3936    4336    5152    5837 
   Size:       1531     147     488     226    1179     400     816     685

Cuts at:   5837     365 
   Size:       2633

  Fragments arranged by size: 

                2633    1531    1179     816     685     488     400     226
                 147
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8105
 
PvuII CAG'CTG

Cuts at:    997    1171    1272    1696    3175    3931    4126    4514    5530 
   Size:        174     101     424    1479     756     195     388    1016

Cuts at:   5530    5890     997 
   Size:        360    3212

  Fragments arranged by size: 

                3212    1479    1016     756     424     388     360     195
                 174     101
 
RsrII CG'GwC_CG

Cuts at:    166    6300     166 
   Size:       6134    1971
 
SacI G_AGCT'C

Cuts at:   1476    3249    5119    1476 
   Size:       1773    1870    4462

  Fragments arranged by size: 

                4462    1870    1773
 
SacII CC_GC'GG

Cuts at:    192     192 
   Size:       8105
 
SalI G'TCGA_C

Cuts at:   1837    5142    1837 
   Size:       3305    4800
 
SapI GCTCTTCn'nnn_

Cuts at:   1489    4551    6128    6338    1489 
   Size:       3062    1577     210    3256

  Fragments arranged by size: 

                3256    3062    1577     210
 
SbfI CC_TGCA'GG

Cuts at:   2757    4336    5152    2757 
   Size:       1579     816    5710

  Fragments arranged by size: 

                5710    1579     816
 
ScaI AGT'ACT

Cuts at:   2834    7622    2834 
   Size:       4788    3317
 
SexAI A'CCwGG_T

Cuts at:   1458    1458 
   Size:       8105
 
SfcI C'TryA_G

Cuts at:     33     361    1571    1892    2039    2527    2570    2753    3701 
   Size:        328    1210     321     147     488      43     183     948

Cuts at:   3701    3932    4332    4515    5148    5833    6801    6992      33 
   Size:        231     400     183     633     685     968     191    1146

  Fragments arranged by size: 

                1210    1146     968     948     685     633     488     400
                 328     321     231     191     183     183     147      43
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   4376    4376 
   Size:       8105
 
SfoI GGC'GCC

Cuts at:   5537    5785    5537 
   Size:        248    7857
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8105
 
SmaI CCC'GGG

Cuts at:    583    1672    4159    5127     583 
   Size:       1089    2487     968    3561

  Fragments arranged by size: 

                3561    2487    1089     968
 
SmlI C'TyrA_G

Cuts at:   1084    2551    4350    5260    6175    6889    7151    1084 
   Size:       1467    1799     910     915     714     262    2038

  Fragments arranged by size: 

                2038    1799    1467     915     910     714     262
 
SpeI A'CTAG_T

Cuts at:     67    1255      67 
   Size:       1188    6917
 
SphI G_CATG'C

Cuts at:   2772    5158    5658    2772 
   Size:       2386     500    5219

  Fragments arranged by size: 

                5219    2386     500
 
StuI AGG'CCT

Cuts at:   4246    4246 
   Size:       8105
 
StyI C'CwwG_G

Cuts at:    107     441    1316    1424    1700    2252    2992    3050    3240 
   Size:        334     875     108     276     552     740      58     190

Cuts at:   3240    3266    3461    3478    3957    4115    4842    5239    5593 
   Size:         26     195      17     479     158     727     397     354

Cuts at:   5593     107 
   Size:       2619

  Fragments arranged by size: 

                2619     875     740     727     552     479     397     354
                 334     276     195     190     158     108      58      26
                  17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1061    1084    3530    4457    5688    5821    6280    6489    1061 
   Size:         23    2446     927    1231     133     459     209    2677

  Fragments arranged by size: 

                2677    2446    1231     927     459     209     133      23
            
 
TatI w'GTAC_w

Cuts at:    673    2832    3192    7620     673 
   Size:       2159     360    4428    1158

  Fragments arranged by size: 

                4428    2159    1158     360
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2216    2248    4170    4202    4263    4295    7622    7654    2216 
   Size:         32    1922      32      61      32    3327      32    2667

  Fragments arranged by size: 

                3327    2667    1922      61      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    136    5902     136 
   Size:       5766    2339
 
XbaI T'CTAG_A

Cuts at:   1770    5136    1770 
   Size:       3366    4739
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    223     722    1404    1965    2438    3982    4496    5600     223 
   Size:        499     682     561     473    1544     514    1104    2728

  Fragments arranged by size: 

                2728    1544    1104     682     561     514     499     473
            
 
XmaI C'CCGG_G

Cuts at:    581    1670    4157    5125     581 
   Size:       1089    2487     968    3561

  Fragments arranged by size: 

                3561    2487    1089     968
 
XmnI GAAnn'nnTTC

Cuts at:   1628    1628 
   Size:       8105
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI   PspOMI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI    AvrII     BmtI
    BsiWI    BsrGI  BstZ17I     ClaI    EcoRV     FseI    FspAI     HpaI
     MfeI     NdeI     NheI     NotI     NruI     PacI     PmlI    PshAI
     PsiI    PspXI    SanDI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
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