Restriction map for pF1KA0595
 (Linear) (Six-base) MAPSORT of: KIAA0595.seq  Check: 302  from: 1  to: 4992



KIAA0595.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     141    1344    2526    2638    4992 
   Size:        141    1203    1182     112    2354

  Fragments arranged by size: 

                2354    1203    1182     141     112
 
AccI GT'mk_AC

Cuts at:      0    1459    1490    1730    2983    4992 
   Size:       1459      31     240    1253    2009

  Fragments arranged by size: 

                2009    1459    1253     240      31
 
Acc65I G'GTAC_C

Cuts at:      0    2212    4992 
   Size:       2212    2780
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     747     973    2192    3281    3482    3808    3815    4992 
   Size:        747     226    1219    1089     201     326       7    1177

  Fragments arranged by size: 

                1219    1177    1089     747     326     226     201       7
            
 
AflIII A'CryG_T

Cuts at:      0    2926    4992 
   Size:       2926    2066
 
AgeI A'CCGG_T

Cuts at:      0    3644    4992 
   Size:       3644    1348
 
AleI CACnn'nnGTG

Cuts at:      0    4727    4992 
   Size:       4727     265
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     418     889    1198    3181    4921    4992 
   Size:        156     262     471     309    1983    1740      71

  Fragments arranged by size: 

                1983    1740     471     309     262     156      71
 
ApaI G_GGCC'C

Cuts at:      0      55     211     563    2942    2956    4015    4992 
   Size:         55     156     352    2379      14    1059     977

  Fragments arranged by size: 

                2379    1059     977     352     156      55      14
 
ApaLI G'TGCA_C

Cuts at:      0    4713    4992 
   Size:       4713     279
 
ApoI r'AATT_y

Cuts at:      0    1599    4933    4992 
   Size:       1599    3334      59

  Fragments arranged by size: 

                3334    1599      59
 
AscI GG'CGCG_CC

Cuts at:      0       7    4992 
   Size:          7    4985
 
AvaI C'yCGr_G

Cuts at:      0      42     473    1562    1574    4049    4992 
   Size:         42     431    1089      12    2475     943

  Fragments arranged by size: 

                2475    1089     943     431      42      12
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1460    1493    4992 
   Size:       1460      33    3499

  Fragments arranged by size: 

                3499    1460      33
 
BanI G'GyrC_C

Cuts at:      0    1311    2212    2538    2826    2851    2889    4992 
   Size:       1311     901     326     288      25      38    2103

  Fragments arranged by size: 

                2103    1311     901     326     288      38      25
 
BanII G_rGCy'C

Cuts at:      0      55      81     211     484     563    1368    1575    2942 
   Size:         55      26     130     273      79     805     207    1367

Cuts at:   2942    2956    3141    3683    3877    3955    4015    4992 
   Size:         14     185     542     194      78      60     977

  Fragments arranged by size: 

                1367     977     805     542     273     207     194     185
                 130      79      78      60      55      26      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2416    2695    4334    4463    4626    4910    4992 
   Size:       2416     279    1639     129     163     284      82

  Fragments arranged by size: 

                2416    1639     284     279     163     129      82
 
BbvCI CC'TCA_GC

Cuts at:      0    1435    4095    4185    4772    4992 
   Size:       1435    2660      90     587     220

  Fragments arranged by size: 

                2660    1435     587     220      90
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4205    4239    4992 
   Size:       4205      34     753

  Fragments arranged by size: 

                4205     753      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     820    4992 
   Size:        820    4172
 
BclI T'GATC_A

Cuts at:      0    1892    4062    4992 
   Size:       1892    2170     930

  Fragments arranged by size: 

                2170    1892     930
 
BfrBI ATG'CAT

Cuts at:      0     828    4037    4992 
   Size:        828    3209     955

  Fragments arranged by size: 

                3209     955     828
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2766    3673    4268    4992 
   Size:       2766     907     595     724

  Fragments arranged by size: 

                2766     907     724     595
 
BglII A'GATC_T

Cuts at:      0     398    4992 
   Size:        398    4594
 
BlpI GC'TnA_GC

Cuts at:      0    1880    4992 
   Size:       1880    3112
 
Bme1580I G_kGCm'C

Cuts at:      0      55     211     563    1208    1316    2609    2831    2892 
   Size:         55     156     352     645     108    1293     222      61

Cuts at:   2892    2942    2956    3061    4015    4717    4992 
   Size:         50      14     105     954     702     275

  Fragments arranged by size: 

                1293     954     702     645     352     275     222     156
                 108     105      61      55      50      14
 
BmgBI CAC'GTC

Cuts at:      0    2083    4992 
   Size:       2083    2909
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     538     557     565    1078    1894    2041    2336    2663 
   Size:        538      19       8     513     816     147     295     327

Cuts at:   2663    2899    4992 
   Size:        236    2093

  Fragments arranged by size: 

                2093     816     538     513     327     295     236     147
                  19       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     501     921    3969    4992 
   Size:        501     420    3048    1023

  Fragments arranged by size: 

                3048    1023     501     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     693    1057    1096    1201    1528    2021    2993 
   Size:        376     317     364      39     105     327     493     972

Cuts at:   2993    3047    3547    3674    4992 
   Size:         54     500     127    1318

  Fragments arranged by size: 

                1318     972     500     493     376     364     327     317
                 127     105      54      39
 
Bpu10I CC'TnA_GC

Cuts at:      0    1435    2492    4095    4185    4772    4992 
   Size:       1435    1057    1603      90     587     220

  Fragments arranged by size: 

                1603    1435    1057     587     220      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     997    2464    4227    4992 
   Size:        997    1467    1763     765

  Fragments arranged by size: 

                1763    1467     997     765
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     640     669    1107    2562    3301    3310    4992 
   Size:        640      29     438    1455     739       9    1682

  Fragments arranged by size: 

                1682    1455     739     640     438      29       9
 
BsaAI yAC'GTr

Cuts at:      0    3448    4992 
   Size:       3448    1544
 
BsaBI GATnn'nnATC

Cuts at:      0    4515    4992 
   Size:       4515     477
 
BsaWI w'CCGG_w

Cuts at:      0      60    3644    3899    4304    4992 
   Size:         60    3584     255     405     688

  Fragments arranged by size: 

                3584     688     405     255      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2108    2125    2138    2155    4155    4185    4992 
   Size:       2108      17      13      17    2000      30     807

  Fragments arranged by size: 

                2108    2000     807      30      17      17      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181     256     474     760     763     859     897    1270 
   Size:        181      75     218     286       3      96      38     373

Cuts at:   1270    2116    3095    3131    3292    3557    4723    4789    4887 
   Size:        846     979      36     161     265    1166      66      98

Cuts at:   4887    4992 
   Size:        105

  Fragments arranged by size: 

                1166     979     846     373     286     265     218     181
                 161     105      98      96      75      66      38      36
                   3
 
BseYI C'CCAG_C

Cuts at:      0     204     270     698     719    1986    2120    2400    2470 
   Size:        204      66     428      21    1267     134     280      70

Cuts at:   2470    3408    4014    4914    4992 
   Size:        938     606     900      78

  Fragments arranged by size: 

                1267     938     900     606     428     280     204     134
                  78      70      66      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    2108    2605    4992 
   Size:        475    1633     497    2387

  Fragments arranged by size: 

                2387    1633     497     475
 
BsiHKAI G_wGCw'C

Cuts at:      0    1127    1368    3141    3382    4717    4992 
   Size:       1127     241    1773     241    1335     275

  Fragments arranged by size: 

                1773    1335    1127     275     241     241
 
BsmI GAATG_Cn'

Cuts at:      0     443    4992 
   Size:        443    4549
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      17    4992 
   Size:         17    4975
 
Bsp1286I G_dGCh'C

Cuts at:      0      55      81     211     484     563    1127    1208    1316 
   Size:         55      26     130     273      79     564      81     108

Cuts at:   1316    1368    1575    2609    2831    2892    2942    2956    3061 
   Size:         52     207    1034     222      61      50      14     105

Cuts at:   3061    3141    3382    3683    3877    3955    4015    4717    4992 
   Size:         80     241     301     194      78      60     702     275

  Fragments arranged by size: 

                1034     702     564     301     275     273     241     222
                 207     194     130     108     105      81      80      79
                  78      61      60      55      52      50      26      14
            
 
BspEI T'CCGG_A

Cuts at:      0      60    3899    4992 
   Size:         60    3839    1093

  Fragments arranged by size: 

                3839    1093      60
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     141     335    1344    2526    2638    4217    4992 
   Size:        141     194    1009    1182     112    1579     775

  Fragments arranged by size: 

                1579    1182    1009     775     194     141     112
 
BsrBI CCG'CTC

Cuts at:      0     140    4505    4992 
   Size:        140    4365     487

  Fragments arranged by size: 

                4365     487     140
 
BsrFI r'CCGG_y

Cuts at:      0    1305    2267    3644    4992 
   Size:       1305     962    1377    1348

  Fragments arranged by size: 

                1377    1348    1305     962
 
BssHII G'CGCG_C

Cuts at:      0       7    4992 
   Size:          7    4985
 
BssSI C'ACGA_G

Cuts at:      0    2128    4992 
   Size:       2128    2864
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     156     498    4593    4992 
   Size:        156     342    4095     399

  Fragments arranged by size: 

                4095     399     342     156
 
BstEII G'GTnAC_C

Cuts at:      0    1212    4992 
   Size:       1212    3780
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     831    2364    3525    4992 
   Size:        831    1533    1161    1467

  Fragments arranged by size: 

                1533    1467    1161     831
 
BstYI r'GATC_y

Cuts at:      0     398     609    1079    4089    4992 
   Size:        398     211     470    3010     903

  Fragments arranged by size: 

                3010     903     470     398     211
 
Bsu36I CC'TnA_GG

Cuts at:      0     842    1019    1511    4133    4984    4992 
   Size:        842     177     492    2622     851       8

  Fragments arranged by size: 

                2622     851     842     492     177       8
 
BtgI C'CryG_G

Cuts at:      0      81    3132    3849    4992 
   Size:         81    3051     717    1143

  Fragments arranged by size: 

                3051    1143     717      81
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1082    4992 
   Size:       1082    3910
 
BtsI GCAGTG_nn'

Cuts at:      0      93     265     735     884    1012    1781    1940    2096 
   Size:         93     172     470     149     128     769     159     156

Cuts at:   2096    2883    3736    4083    4992 
   Size:        787     853     347     909

  Fragments arranged by size: 

                 909     853     787     769     470     347     172     159
                 156     149     128      93
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1770    1805    2669    2704    2984    3019    4992 
   Size:       1770      35     864      35     280      35    1973

  Fragments arranged by size: 

                1973    1770     864     280      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3207    4992 
   Size:       3207    1785
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1640    3895    4992 
   Size:       1640    2255    1097

  Fragments arranged by size: 

                2255    1640    1097
 
EaeI y'GGCC_r

Cuts at:      0     755     768    3129    3665    3743    4653    4800    4992 
   Size:        755      13    2361     536      78     910     147     192

  Fragments arranged by size: 

                2361     910     755     536     192     147      78      13
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     608     657     737    1381    2297    3625    4443    4449 
   Size:        608      49      80     644     916    1328     818       6

Cuts at:   4449    4470    4992 
   Size:         21     522

  Fragments arranged by size: 

                1328     916     818     644     608     522      80      49
                  21       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    4016    4992 
   Size:       4016     976
 
EcoICRI GAG'CTC

Cuts at:      0    1366    3139    4992 
   Size:       1366    1773    1853

  Fragments arranged by size: 

                1853    1773    1366
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     693     747     973    1057    1096    1201    1528 
   Size:        376     317      54     226      84      39     105     327

Cuts at:   1528    2021    2192    2993    3047    3281    3482    3547    3674 
   Size:        493     171     801      54     234     201      65     127

Cuts at:   3674    3808    3815    4992 
   Size:        134       7    1177

  Fragments arranged by size: 

                1177     801     493     376     327     317     234     226
                 201     171     134     127     105      84      65      54
                  54      39       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     316    4992 
   Size:        316    4676
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51      52     228     559    1657    2467    2939    2952 
   Size:         51       1     176     331    1098     810     472      13

Cuts at:   2952    2959    3635    3908    4367    4992 
   Size:          7     676     273     459     625

  Fragments arranged by size: 

                1098     810     676     625     472     459     331     273
                 176      51      13       7       1
 
EcoRI G'AATT_C

Cuts at:      0    1599    4992 
   Size:       1599    3393
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3357    3447    4454    4992 
   Size:       3357      90    1007     538

  Fragments arranged by size: 

                3357    1007     538      90
 
HaeII r_GCGC'y

Cuts at:      0     115     134    3975    4992 
   Size:        115      19    3841    1017

  Fragments arranged by size: 

                3841    1017     115      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      14      46    1225    1257    2125    2157    3090    3122 
   Size:         14      32    1179      32     868      32     933      32

Cuts at:   3122    3225    3257    3271    3303    3950    3982    4876    4908 
   Size:        103      32      14      32     647      32     894      32

Cuts at:   4908    4992 
   Size:         84

  Fragments arranged by size: 

                1179     933     894     868     647     103      84      32
                  32      32      32      32      32      32      32      14
                  14
 
HincII GTy'rAC

Cuts at:      0     549    1731    1761    1821    1836    1926    2013    2043 
   Size:        549    1182      30      60      15      90      87      30

Cuts at:   2043    3615    4992 
   Size:       1572    1377

  Fragments arranged by size: 

                1572    1377    1182     549      90      87      60      30
                  30      15
 
HindIII A'AGCT_T

Cuts at:      0     661    4992 
   Size:        661    4331
 
KpnI G_GTAC'C

Cuts at:      0    2216    4992 
   Size:       2216    2776
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    4325    4919    4992 
   Size:       4325     594      73

  Fragments arranged by size: 

                4325     594      73
 
MscI TGG'CCA

Cuts at:      0     757     770    3131    3667    3745    4802    4992 
   Size:        757      13    2361     536      78    1057     190

  Fragments arranged by size: 

                2361    1057     757     536     190      78      13
 
MslI CAynn'nnrTG

Cuts at:      0    4727    4992 
   Size:       4727     265
 
MspA1I CmG'CkG

Cuts at:      0      83     889    1063    1164    1588    3067    3823    4018 
   Size:         83     806     174     101     424    1479     756     195

Cuts at:   4018    4197    4406    4992 
   Size:        179     209     586

  Fragments arranged by size: 

                1479     806     756     586     424     209     195     179
                 174     101      83
 
NcoI C'CATG_G

Cuts at:      0    3132    3849    4992 
   Size:       3132     717    1143

  Fragments arranged by size: 

                3132    1143     717
 
NsiI A_TGCA'T

Cuts at:      0     830    4039    4992 
   Size:        830    3209     953

  Fragments arranged by size: 

                3209     953     830
 
NspI r_CATG'y

Cuts at:      0    2664    2930    2971    3251    4992 
   Size:       2664     266      41     280    1741

  Fragments arranged by size: 

                2664    1741     280     266      41
 
PasI CC'CwG_GG

Cuts at:      0    1289    1825    2103    2956    3326    4992 
   Size:       1289     536     278     853     370    1666

  Fragments arranged by size: 

                1666    1289     853     536     370     278
 
PciI A'CATG_T

Cuts at:      0    2926    4992 
   Size:       2926    2066
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     889    4992 
   Size:        889    4103
 
PfoI T'CCnGG_A

Cuts at:      0     812    1107    1127    4992 
   Size:        812     295      20    3865

  Fragments arranged by size: 

                3865     812     295      20
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     518     550    4992 
   Size:        518      32    4442

  Fragments arranged by size: 

                4442     518      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     228    2467    3908    4367    4992 
   Size:        228    2239    1441     459     625

  Fragments arranged by size: 

                2239    1441     625     459     228
 
PspOMI G'GGCC_C

Cuts at:      0      51     207     559    2938    2952    4011    4992 
   Size:         51     156     352    2379      14    1059     981

  Fragments arranged by size: 

                2379    1059     981     352     156      51      14
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3441    3473    4992 
   Size:       3441      32    1519

  Fragments arranged by size: 

                3441    1519      32
 
PstI C_TGCA'G

Cuts at:      0     257    1788    1935    2423    2649    3828    4228    4992 
   Size:        257    1531     147     488     226    1179     400     764

  Fragments arranged by size: 

                1531    1179     764     488     400     257     226     147
            
 
PvuII CAG'CTG

Cuts at:      0     889    1063    1164    1588    3067    3823    4018    4406 
   Size:        889     174     101     424    1479     756     195     388

Cuts at:   4406    4992 
   Size:        586

  Fragments arranged by size: 

                1479     889     756     586     424     388     195     174
                 101
 
RsrII CG'GwC_CG

Cuts at:      0      58    4992 
   Size:         58    4934
 
SacI G_AGCT'C

Cuts at:      0    1368    3141    4992 
   Size:       1368    1773    1851

  Fragments arranged by size: 

                1851    1773    1368
 
SacII CC_GC'GG

Cuts at:      0      84    4992 
   Size:         84    4908
 
SalI G'TCGA_C

Cuts at:      0    1729    4992 
   Size:       1729    3263
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1381    4443    4992 
   Size:       1381    3062     549

  Fragments arranged by size: 

                3062    1381     549
 
SbfI CC_TGCA'GG

Cuts at:      0    2649    4228    4992 
   Size:       2649    1579     764

  Fragments arranged by size: 

                2649    1579     764
 
ScaI AGT'ACT

Cuts at:      0    2726    4992 
   Size:       2726    2266
 
SexAI A'CCwGG_T

Cuts at:      0    1350    4992 
   Size:       1350    3642
 
SfcI C'TryA_G

Cuts at:      0     253    1463    1784    1931    2419    2462    2645    3593 
   Size:        253    1210     321     147     488      43     183     948

Cuts at:   3593    3824    4224    4407    4992 
   Size:        231     400     183     585

  Fragments arranged by size: 

                1210     948     585     488     400     321     253     231
                 183     183     147      43
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    4268    4992 
   Size:       4268     724
 
SmaI CCC'GGG

Cuts at:      0     475    1564    4051    4992 
   Size:        475    1089    2487     941

  Fragments arranged by size: 

                2487    1089     941     475
 
SmlI C'TyrA_G

Cuts at:      0     976    2443    4242    4992 
   Size:        976    1467    1799     750

  Fragments arranged by size: 

                1799    1467     976     750
 
SpeI A'CTAG_T

Cuts at:      0    1147    4992 
   Size:       1147    3845
 
SphI G_CATG'C

Cuts at:      0    2664    4992 
   Size:       2664    2328
 
StuI AGG'CCT

Cuts at:      0    4138    4992 
   Size:       4138     854
 
StyI C'CwwG_G

Cuts at:      0     333    1208    1316    1592    2144    2884    2942    3132 
   Size:        333     875     108     276     552     740      58     190

Cuts at:   3132    3158    3353    3370    3849    4007    4734    4992 
   Size:         26     195      17     479     158     727     258

  Fragments arranged by size: 

                 875     740     727     552     479     333     276     258
                 195     190     158     108      58      26      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     953     976    3422    4349    4992 
   Size:        953      23    2446     927     643

  Fragments arranged by size: 

                2446     953     927     643      23
 
TatI w'GTAC_w

Cuts at:      0     565    2724    3084    4992 
   Size:        565    2159     360    1908

  Fragments arranged by size: 

                2159    1908     565     360
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2108    2140    4062    4094    4155    4187    4992 
   Size:       2108      32    1922      32      61      32     805

  Fragments arranged by size: 

                2108    1922     805      61      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      28    4992 
   Size:         28    4964
 
XbaI T'CTAG_A

Cuts at:      0    1662    4992 
   Size:       1662    3330
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     115     614    1296    1857    2330    3874    4388    4992 
   Size:        115     499     682     561     473    1544     514     604

  Fragments arranged by size: 

                1544     682     604     561     514     499     473     115
            
 
XmaI C'CCGG_G

Cuts at:      0     473    1562    4049    4992 
   Size:        473    1089    2487     943

  Fragments arranged by size: 

                2487    1089     943     473
 
XmnI GAAnn'nnTTC

Cuts at:      0    1520    4992 
   Size:       1520    3472
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AgeI     AleI    AlwNI
     ApaI    ApaLI     ApoI     AscI     AvaI     BaeI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI
   PspOMI     PsrI     PstI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AhdI     AlfI     AloI     AseI
    AsiSI    AvrII    BamHI     BbeI     BmtI    BsaHI    BsiEI    BsiWI
    BspHI    BsrDI    BsrGI    BstBI  BstZ17I     ClaI     DraI     EagI
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PmeI     PmlI    PshAI     PsiI    PspXI     PvuI    SanDI     SfoI
     SgfI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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