Restriction map for pF1KA0589
 (Circular) (Six-base) MAPSORT of: pF1KA0589.seq  Check: 4230  from: 1  to: 5117



pF1KA0589  5117 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1830    1983    1830 
   Size:        153    4964
 
AatII G_ACGT'C

Cuts at:   1738    1738 
   Size:       5117
 
AccI GT'mk_AC

Cuts at:   2155    2155 
   Size:       5117
 
Acc65I G'GTAC_C

Cuts at:    120    2133     120 
   Size:       2013    3104
 
AclI AA'CG_TT

Cuts at:   4957    4957 
   Size:       5117
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    364     975    1477    2942    3374    4121    4389     364 
   Size:        611     502    1465     432     747     268    1092

  Fragments arranged by size: 

                1465    1092     747     611     502     432     268
 
AfeI AGC'GCT

Cuts at:    141    1408     141 
   Size:       1267    3850
 
AflIII A'CryG_T

Cuts at:   2469    3573    4550    2469 
   Size:       1104     977    3036

  Fragments arranged by size: 

                3036    1104     977
 
AgeI A'CCGG_T

Cuts at:   3505    3505 
   Size:       5117
 
AhdI GACnn_n'nnGTC

Cuts at:   1374    1374 
   Size:       5117
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    679     711     679 
   Size:         32    5085
 
AlwNI CAG_nnn'CTG

Cuts at:    708    2422    3989     708 
   Size:       1714    1567    1836

  Fragments arranged by size: 

                1836    1714    1567
 
ApaI G_GGCC'C

Cuts at:    262    1520    1590     262 
   Size:       1258      70    3789

  Fragments arranged by size: 

                3789    1258      70
 
ApaLI G'TGCA_C

Cuts at:   1075    3887    1075 
   Size:       2812    2305
 
ApoI r'AATT_y

Cuts at:   2121    4470    4726    2121 
   Size:       2349     256    2512

  Fragments arranged by size: 

                2512    2349     256
 
AscI GG'CGCG_CC

Cuts at:    891     891 
   Size:       5117
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5117
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5117
 
AvaI C'yCGr_G

Cuts at:    615     961    2137     615 
   Size:        346    1176    3595

  Fragments arranged by size: 

                3595    1176     346
 
BamHI G'GATC_C

Cuts at:    554    1244    2142     554 
   Size:        690     898    3529

  Fragments arranged by size: 

                3529     898     690
 
BanI G'GyrC_C

Cuts at:    120     349     619    1117    1198    1915    2133    2547    2795 
   Size:        229     270     498      81     717     218     414     248

Cuts at:   2795    2830     120 
   Size:         35    2407

  Fragments arranged by size: 

                2407     717     498     414     270     248     229     218
                  81      35
 
BanII G_rGCy'C

Cuts at:    262     394     548     877     971    1520    1590    2131    3161 
   Size:        132     154     329      94     549      70     541    1030

Cuts at:   3161     262 
   Size:       2218

  Fragments arranged by size: 

                2218    1030     549     541     329     154     132      94
                  70
 
BbeI G_GCGC'C

Cuts at:    623    1121    1202    2551    2799     623 
   Size:        498      81    1349     248    2941

  Fragments arranged by size: 

                2941    1349     498     248      81
 
BbsI GAAGACnn'nnnn_

Cuts at:    331     331 
   Size:       5117
 
BbvCI CC'TCA_GC

Cuts at:    143     143 
   Size:       5117
 
BciVI GTATCCnnnnn_n'

Cuts at:   3015    3776    5117    3015 
   Size:        761    1341    3015

  Fragments arranged by size: 

                3015    1341     761
 
BclI T'GATC_A

Cuts at:   2637    2637 
   Size:       5117
 
BglI GCCn_nnn'nGGC

Cuts at:    966    1534    1951    1984    2086     966 
   Size:        568     417      33     102    3997

  Fragments arranged by size: 

                3997     568     417     102      33
 
BglII A'GATC_T

Cuts at:   2632    4669    2632 
   Size:       2037    3080
 
BlpI GC'TnA_GC

Cuts at:   2229    2229 
   Size:       5117
 
Bme1580I G_kGCm'C

Cuts at:    161     262    1079    1520    1590    2742    2835    3891     161 
   Size:        101     817     441      70    1152      93    1056    1387

  Fragments arranged by size: 

                1387    1152    1056     817     441     101      93      70
            
 
BmgBI CAC'GTC

Cuts at:    411     411 
   Size:       5117
 
BmrI ACTGGGnnnn_n'

Cuts at:   1563    2329    2410    2745    4889    1563 
   Size:        766      81     335    2144    1791

  Fragments arranged by size: 

                2144    1791     766     335      81
 
BmtI G_CTAG'C

Cuts at:   1501    1501 
   Size:       5117
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3484    3484 
   Size:       5117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    136    1354    1627     136 
   Size:       1218     273    3626

  Fragments arranged by size: 

                3626    1218     273
 
Bpu10I CC'TnA_GC

Cuts at:    143     388    1715     143 
   Size:        245    1327    3545

  Fragments arranged by size: 

                3545    1327     245
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    841     853    2293    3172    3664    3962     841 
   Size:         12    1440     879     492     298    1996

  Fragments arranged by size: 

                1996    1440     879     492     298      12
 
BsaI GGTCTCn'nnnn_

Cuts at:   4821    4821 
   Size:       5117
 
BsaAI yAC'GTr

Cuts at:    432    2470     432 
   Size:       2038    3079
 
BsaBI GATnn'nnATC

Cuts at:      6    2631    4668       6 
   Size:       2625    2037     455

  Fragments arranged by size: 

                2625    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    620     980    1118    1199    1735    2548    2796    3498    4994 
   Size:        360     138      81     536     813     248     702    1496

Cuts at:   4994     620 
   Size:        743

  Fragments arranged by size: 

                1496     813     743     702     536     360     248     138
                  81
 
BsaWI w'CCGG_w

Cuts at:    123    2306    2827    3505    3779    3926    4337     123 
   Size:       2183     521     678     274     147     411     903

  Fragments arranged by size: 

                2183     903     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    157     187     376     406     562     592     679     709    1555 
   Size:         30     189      30     156      30      87      30     846

Cuts at:   1555    1585    1837    1867     157 
   Size:         30     252      30    3407

  Fragments arranged by size: 

                3407     846     252     189     156      87      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    324    1440    1576    1813    1897    1978    2011    2055     324 
   Size:       1116     136     237      84      81      33      44    3386

  Fragments arranged by size: 

                3386    1116     237     136      84      81      44      33
            
 
BseYI C'CCAG_C

Cuts at:    360     454    1068    1390    1512    2045    2542    3300    3877 
   Size:         94     614     322     122     533     497     758     577

Cuts at:   3877    4373     360 
   Size:        496    1104

  Fragments arranged by size: 

                1104     758     614     577     533     497     496     322
                 122      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    805    1267    1623    1858    1875     805 
   Size:        462     356     235      17    4047

  Fragments arranged by size: 

                4047     462     356     235      17
 
BsiEI CG_ry'CG

Cuts at:    104    1263    1913    2659    2705    3913     104 
   Size:       1159     650     746      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1159     746     650      46
 
BsiHKAI G_wGCw'C

Cuts at:    394     416     548    1079    1129    1340    2131    2909    3099 
   Size:         22     132     531      50     211     791     778     190

Cuts at:   3099    3891     394 
   Size:        792    1620

  Fragments arranged by size: 

                1620     792     791     778     531     211     190     132
                  50      22
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1726    1726 
   Size:       5117
 
Bsp1286I G_dGCh'C

Cuts at:    161     262     394     416     548     877     971    1079    1129 
   Size:        101     132      22     132     329      94     108      50

Cuts at:   1129    1340    1520    1590    2131    2742    2835    2909    3099 
   Size:        211     180      70     541     611      93      74     190

Cuts at:   3099    3161    3891     161 
   Size:         62     730    1387

  Fragments arranged by size: 

                1387     730     611     541     329     211     190     180
                 132     132     108     101      94      93      74      70
                  62      50      22
 
BspEI T'CCGG_A

Cuts at:   4337    4337 
   Size:       5117
 
BspHI T'CATG_A

Cuts at:    818    1421    4293     818 
   Size:        603    2872    1642

  Fragments arranged by size: 

                2872    1642     603
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    190    1586    1830    1983    2167    2381    2683    3064     190 
   Size:       1396     244     153     184     214     302     381    2243

  Fragments arranged by size: 

                2243    1396     381     302     244     214     184     153
            
 
BsrBI CCG'CTC

Cuts at:     50     188    1104    1272    1557    1719    2061    3409    3463 
   Size:        138     916     168     285     162     342    1348      54

Cuts at:   3463    4947    5112      50 
   Size:       1484     165      55

  Fragments arranged by size: 

                1484    1348     916     342     285     168     165     162
                 138      55      54
 
BsrDI GCAATG_nn'

Cuts at:    594    1676    3029     594 
   Size:       1082    1353    2682

  Fragments arranged by size: 

                2682    1353    1082
 
BsrFI r'CCGG_y

Cuts at:   1619    1912    3115    3296    3505    1619 
   Size:        293    1203     181     209    3231

  Fragments arranged by size: 

                3231    1203     293     209     181
 
BssHII G'CGCG_C

Cuts at:    891    1204     891 
   Size:        313    4804
 
BssSI C'ACGA_G

Cuts at:   1093    3388    3746    1093 
   Size:       2295     358    2464

  Fragments arranged by size: 

                2464    2295     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    708     957    2494     708 
   Size:        249    1537    3331

  Fragments arranged by size: 

                3331    1537     249
 
BstBI TT'CG_AA

Cuts at:   1655    3478    1655 
   Size:       1823    3294
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     360    1179     114 
   Size:        246     819    4052

  Fragments arranged by size: 

                4052     819     246
 
BstYI r'GATC_y

Cuts at:      1     554    1244    1544    1818    2142    2609    2632    2967 
   Size:        553     690     300     274     324     467      23     335

Cuts at:   2967    3213    4225    4311    4323    4669       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     690     553     467     449     346     335     324
                 300     274     246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     267     377     745    1172    1178    1263    1747    1808 
   Size:        160     110     368     427       6      85     484      61

Cuts at:   1808     107 
   Size:       3416

  Fragments arranged by size: 

                3416     484     427     368     160     110      85      61
                   6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3067    3248    3411    3067 
   Size:        181     163    4773

  Fragments arranged by size: 

                4773     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2360    2360 
   Size:       5117
 
DraI TTT'AAA

Cuts at:   2116    2116 
   Size:       5117
 
DrdI GACnn_nn'nnGTC

Cuts at:   1027    2823    3681    1027 
   Size:       1796     858    2463

  Fragments arranged by size: 

                2463    1796     858
 
EaeI y'GGCC_r

Cuts at:    264     559    1175    1260    1535    1910    2702    2876    3267 
   Size:        295     616      85     275     375     792     174     391

Cuts at:   3267    3294    4639     264 
   Size:         27    1345     742

  Fragments arranged by size: 

                1345     792     742     616     391     375     295     275
                 174      85      27
 
EagI C'GGCC_G

Cuts at:   1260    1910    2702    1260 
   Size:        650     792    3675

  Fragments arranged by size: 

                3675     792     650
 
EarI CTCTTCn'nnn_

Cuts at:    640     910    1808    3140    3350     640 
   Size:        270     898    1332     210    2407

  Fragments arranged by size: 

                2407    1332     898     270     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    148    1502    3325    3635    3781    4927     148 
   Size:       1354    1823     310     146    1146     338

  Fragments arranged by size: 

                1823    1354    1146     338     310     146
 
EcoICRI GAG'CTC

Cuts at:    392     546    2129     392 
   Size:        154    1583    3380

  Fragments arranged by size: 

                3380    1583     154
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     364     975    1354    1477    1627    2942    3374    4121 
   Size:        228     611     379     123     150    1315     432     747

Cuts at:   4121    4389     136 
   Size:        268     864

  Fragments arranged by size: 

                1315     864     747     611     432     379     268     228
                 150     123
 
EcoO109I rG'GnC_Cy

Cuts at:    259     936    1350    1516    1586    2079    2256     259 
   Size:        677     414     166      70     493     177    3120

  Fragments arranged by size: 

                3120     677     493     414     177     166      70
 
EcoRI G'AATT_C

Cuts at:   2121    2121 
   Size:       5117
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3136    3136 
   Size:       5117
 
FseI GG_CCGG'CC

Cuts at:   1623    1623 
   Size:       5117
 
FspI TGC'GCA

Cuts at:   2898    2898 
   Size:       5117
 
HaeII r_GCGC'y

Cuts at:    143     623     998    1121    1165    1202    1410    2075    2551 
   Size:        480     375     123      44      37     208     665     476

Cuts at:   2551    2799    3821    4800     143 
   Size:        248    1022     979     460

  Fragments arranged by size: 

                1022     979     665     480     476     460     375     248
                 208     123      44      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    223     255    1360    1392    2959    2989    2991    3021     223 
   Size:         32    1105      32    1567      30       2      30    2319

  Fragments arranged by size: 

                2319    1567    1105      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2156    2156 
   Size:       5117
 
KasI G'GCGC_C

Cuts at:    619    1117    1198    2547    2795     619 
   Size:        498      81    1349     248    2941

  Fragments arranged by size: 

                2941    1349     498     248      81
 
KpnI G_GTAC'C

Cuts at:    124    2137     124 
   Size:       2013    3104
 
MluI A'CGCG_T

Cuts at:   4550    4550 
   Size:       5117
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    635    1718    2117    3788    3972     635 
   Size:       1083     399    1671     184    1780

  Fragments arranged by size: 

                1780    1671    1083     399     184
 
MscI TGG'CCA

Cuts at:    266    2878     266 
   Size:       2612    2505
 
MslI CAynn'nnrTG

Cuts at:    290    1177    3106    3233     290 
   Size:        887    1929     127    2174

  Fragments arranged by size: 

                2174    1929     887     127
 
MspA1I CmG'CkG

Cuts at:    360     597     747    1017    1252    1265    1512    1525    1749 
   Size:        237     150     270     235      13     247      13     224

Cuts at:   1749    1810    2228    2542    2902    3915    4160     360 
   Size:         61     418     314     360    1013     245    1317

  Fragments arranged by size: 

                1317    1013     418     360     314     270     247     245
                 237     235     224     150      61      13      13
 
NaeI GCC'GGC

Cuts at:   1621    3298    1621 
   Size:       1677    3440
 
NarI GG'CG_CC

Cuts at:    620    1118    1199    2548    2796     620 
   Size:        498      81    1349     248    2941

  Fragments arranged by size: 

                2941    1349     498     248      81
 
NcoI C'CATG_G

Cuts at:    107     267    1178     107 
   Size:        160     911    4046

  Fragments arranged by size: 

                4046     911     160
 
NgoMIV G'CCGG_C

Cuts at:   1619    3296    1619 
   Size:       1677    3440
 
NheI G'CTAG_C

Cuts at:   1497    1497 
   Size:       5117
 
NspI r_CATG'y

Cuts at:    960    2170    2670    3577     960 
   Size:       1210     500     907    2500

  Fragments arranged by size: 

                2500    1210     907     500
 
PciI A'CATG_T

Cuts at:   3573    3573 
   Size:       5117
 
PfoI T'CCnGG_A

Cuts at:    491     539    1031     491 
   Size:         48     492    4577

  Fragments arranged by size: 

                4577     492      48
 
PmeI GTTT'AAAC

Cuts at:   2116    2116 
   Size:       5117
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    679     711    4256    4288     679 
   Size:         32    3545      32    1508

  Fragments arranged by size: 

                3545    1508      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    936     936 
   Size:       5117
 
PspOMI G'GGCC_C

Cuts at:    258    1516    1586     258 
   Size:       1258      70    3789

  Fragments arranged by size: 

                3789    1258      70
 
PstI C_TGCA'G

Cuts at:    704    1016    1310    1826    2164    2849     704 
   Size:        312     294     516     338     685    2972

  Fragments arranged by size: 

                2972     685     516     338     312     294
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5117
 
PvuII CAG'CTG

Cuts at:    360    2542    2902     360 
   Size:       2182     360    2575

  Fragments arranged by size: 

                2575    2182     360
 
RsrII CG'GwC_CG

Cuts at:   3312    3312 
   Size:       5117
 
SacI G_AGCT'C

Cuts at:    394     548    2131     394 
   Size:        154    1583    3380

  Fragments arranged by size: 

                3380    1583     154
 
SacII CC_GC'GG

Cuts at:    748    1266    1750    1811     748 
   Size:        518     484      61    4054

  Fragments arranged by size: 

                4054     518     484      61
 
SalI G'TCGA_C

Cuts at:   2154    2154 
   Size:       5117
 
SapI GCTCTTCn'nnn_

Cuts at:    910    3140    3350     910 
   Size:       2230     210    2677

  Fragments arranged by size: 

                2677    2230     210
 
SbfI CC_TGCA'GG

Cuts at:   2164    2164 
   Size:       5117
 
ScaI AGT'ACT

Cuts at:   4634    4634 
   Size:       5117
 
SfcI C'TryA_G

Cuts at:     33     700    1012    1056    1306    1314    1667    1822    2067 
   Size:        667     312      44     250       8     353     155     245

Cuts at:   2067    2160    2845    3838    4029      33 
   Size:         93     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     667     353     312     250     245
                 191     155      93      44       8
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2086    2086 
   Size:       5117
 
SfoI GGC'GCC

Cuts at:    621    1119    1200    2549    2797     621 
   Size:        498      81    1349     248    2941

  Fragments arranged by size: 

                2941    1349     498     248      81
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5117
 
SmaI CCC'GGG

Cuts at:    617    2139     617 
   Size:       1522    3595
 
SmlI C'TyrA_G

Cuts at:    856     868    2272    3187    3679    3941     856 
   Size:         12    1404     915     492     262    2032

  Fragments arranged by size: 

                2032    1404     915     492     262      12
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5117
 
SphI G_CATG'C

Cuts at:   2170    2670    2170 
   Size:        500    4617
 
StuI AGG'CCT

Cuts at:   1954    1987    1954 
   Size:         33    5084
 
StyI C'CwwG_G

Cuts at:    107     267    1178    2251    2605     107 
   Size:        160     911    1073     354    2619

  Fragments arranged by size: 

                2619    1073     911     354     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2037    2700    2833    3292    3501    2037 
   Size:        663     133     459     209    3653

  Fragments arranged by size: 

                3653     663     459     209     133
 
TatI w'GTAC_w

Cuts at:    575     776    2051    4632     575 
   Size:        201    1275    2581    1060

  Fragments arranged by size: 

                2581    1275    1060     201
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    157     189     376     408    1837    1869    4634    4666     157 
   Size:         32     187      32    1429      32    2765      32     608

  Fragments arranged by size: 

                2765    1429     608     187      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1689    2914    1689 
   Size:       1225    3892
 
XbaI T'CTAG_A

Cuts at:   2148    2148 
   Size:       5117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2612    2612 
   Size:       5117
 
XmaI C'CCGG_G

Cuts at:    615    2137     615 
   Size:       1522    3595
 
XmnI GAAnn'nnTTC

Cuts at:    919     919 
   Size:       5117
 
ZraI GAC'GTC

Cuts at:   1736    1736 
   Size:       5117
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FseI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PciI
     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AleI     AlfI    AvrII     BaeI     BcgI    BfrBI    BsiWI
     BsmI    BsrGI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV    FspAI  HindIII     HpaI     MfeI     NdeI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PshAI     PsiI
    PspXI     PsrI    SanDI    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com