Restriction map for pF1KA0571
 (Circular) (Six-base) MAPSORT of: pF1KA0571.seq  Check: 9335  from: 1  to: 5027



pF1KA0571  5027 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1387    1387 
   Size:       5027
 
AccI GT'mk_AC

Cuts at:    926    1418    2065     926 
   Size:        492     647    3888

  Fragments arranged by size: 

                3888     647     492
 
Acc65I G'GTAC_C

Cuts at:   2043    2043 
   Size:       5027
 
AclI AA'CG_TT

Cuts at:   4867    4867 
   Size:       5027
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1358    2852    3284    3637    4299    1358 
   Size:       1494     432     353     662    2086

  Fragments arranged by size: 

                2086    1494     662     432     353
 
AflIII A'CryG_T

Cuts at:   2379    4179    4460    2379 
   Size:       1800     281    2946

  Fragments arranged by size: 

                2946    1800     281
 
AhdI GACnn_n'nnGTC

Cuts at:    703     703 
   Size:       5027
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    218     218 
   Size:       5027
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    136     168     136 
   Size:         32    4995
 
AlwNI CAG_nnn'CTG

Cuts at:   1768    1953    2332    3770    1768 
   Size:        185     379    1438    3025

  Fragments arranged by size: 

                3025    1438     379     185
 
ApaI G_GGCC'C

Cuts at:   1443    1443 
   Size:       5027
 
ApaLI G'TGCA_C

Cuts at:   1486    3865    1486 
   Size:       2379    2648
 
ApoI r'AATT_y

Cuts at:    772    2031    4380    4636     772 
   Size:       1259    2349     256    1163

  Fragments arranged by size: 

                2349    1259    1163     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5027
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5027
 
AvaI C'yCGr_G

Cuts at:   2047    2047 
   Size:       5027
 
AvrII C'CTAG_G

Cuts at:    973     973 
   Size:       5027
 
BamHI G'GATC_C

Cuts at:   2052    2052 
   Size:       5027
 
BanI G'GyrC_C

Cuts at:    634    2011    2043    2457    2705    2740     634 
   Size:       1377      32     414     248      35    2921

  Fragments arranged by size: 

                2921    1377     414     248      35      32
 
BanII G_rGCy'C

Cuts at:    428    1435    1443    1861    2041    3071     428 
   Size:       1007       8     418     180    1030    2384

  Fragments arranged by size: 

                2384    1030    1007     418     180       8
 
BbeI G_GCGC'C

Cuts at:   2461    2709    2461 
   Size:        248    4779
 
BbsI GAAGACnn'nnnn_

Cuts at:   1346    1948    1346 
   Size:        602    4425
 
BbvCI CC'TCA_GC

Cuts at:    356     390     468    1100     356 
   Size:         34      78     632    4283

  Fragments arranged by size: 

                4283     632      78      34
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    891     925    1119    1153     891 
   Size:         34     194      34    4765

  Fragments arranged by size: 

                4765     194      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2925    3981    5027    2925 
   Size:       1056    1046    2925

  Fragments arranged by size: 

                2925    1056    1046
 
BclI T'GATC_A

Cuts at:   2547    2547 
   Size:       5027
 
BglI GCCn_nnn'nGGC

Cuts at:    759     842     759 
   Size:         83    4944
 
BglII A'GATC_T

Cuts at:    335    1132    2542    4579     335 
   Size:        797    1410    2037     783

  Fragments arranged by size: 

                2037    1410     797     783
 
BlpI GC'TnA_GC

Cuts at:    422     427    2139     422 
   Size:          5    1712    3310

  Fragments arranged by size: 

                3310    1712       5
 
Bme1580I G_kGCm'C

Cuts at:    637    1355    1443    1490    2652    2745    3869     637 
   Size:        718      88      47    1162      93    1124    1795

  Fragments arranged by size: 

                1795    1162    1124     718      93      88      47
 
BmgBI CAC'GTC

Cuts at:   1983    1983 
   Size:       5027
 
BmrI ACTGGGnnnn_n'

Cuts at:   1671    1756    1778    2239    2320    2655    4799    1671 
   Size:         85      22     461      81     335    2144    1899

  Fragments arranged by size: 

                2144    1899     461     335      85      81      22
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3394    3394 
   Size:       5027
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    401     468    1650     401 
   Size:         67    1182    3778

  Fragments arranged by size: 

                3778    1182      67
 
Bpu10I CC'TnA_GC

Cuts at:    356     390     468    1100     356 
   Size:         34      78     632    4283

  Fragments arranged by size: 

                4283     632      78      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2203    3082    3796    4094    2203 
   Size:        879     714     298    3136

  Fragments arranged by size: 

                3136     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1101    1207    4731    1101 
   Size:        106    3524    1397

  Fragments arranged by size: 

                3524    1397     106
 
BsaAI yAC'GTr

Cuts at:   2380    2380 
   Size:       5027
 
BsaBI GATnn'nnATC

Cuts at:      6    2541    4578       6 
   Size:       2535    2037     455

  Fragments arranged by size: 

                2535    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    883    2458    2706    3408    4904     883 
   Size:       1575     248     702    1496    1006

  Fragments arranged by size: 

                1575    1496    1006     702     248
 
BsaWI w'CCGG_w

Cuts at:   2216    2737    3415    3826    3973    4247    2216 
   Size:        521     678     411     147     274    2996

  Fragments arranged by size: 

                2996     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    136     166    1654    1684     136 
   Size:         30    1488      30    3479

  Fragments arranged by size: 

                3479    1488      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    373     672    1662     373 
   Size:        299     990    3738

  Fragments arranged by size: 

                3738     990     299
 
BseYI C'CCAG_C

Cuts at:    886    1498    2452    3210    3875    4283     886 
   Size:        612     954     758     665     408    1630

  Fragments arranged by size: 

                1630     954     758     665     612     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    921    1268     921 
   Size:        347    4680
 
BsiEI CG_ry'CG

Cuts at:    104    1321    2569    2615    3845     104 
   Size:       1217    1248      46    1230    1286

  Fragments arranged by size: 

                1286    1248    1230    1217      46
 
BsiHKAI G_wGCw'C

Cuts at:    367     428    1490    1819    1861    2041    2819    3009    3869 
   Size:         61    1062     329      42     180     778     190     860

Cuts at:   3869     367 
   Size:       1525

  Fragments arranged by size: 

                1525    1062     860     778     329     190     180      61
                  42
 
Bsp1286I G_dGCh'C

Cuts at:    367     428     637    1355    1435    1443    1490    1819    1861 
   Size:         61     209     718      80       8      47     329      42

Cuts at:   1861    2041    2652    2745    2819    3009    3071    3869     367 
   Size:        180     611      93      74     190      62     798    1525

  Fragments arranged by size: 

                1525     798     718     611     329     209     190     180
                  93      80      74      62      61      47      42       8
            
 
BspHI T'CATG_A

Cuts at:    653    3459     653 
   Size:       2806    2221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    186    1387    2077    2291    2593    2974     186 
   Size:       1201     690     214     302     381    2239

  Fragments arranged by size: 

                2239    1201     690     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     113     410    3319    3373    4857    5022      50 
   Size:         63     297    2909      54    1484     165      55

  Fragments arranged by size: 

                2909    1484     297     165      63      55      54
 
BsrDI GCAATG_nn'

Cuts at:    699    1181    2939     699 
   Size:        482    1758    2787

  Fragments arranged by size: 

                2787    1758     482
 
BsrFI r'CCGG_y

Cuts at:    394     758    1253    1819    3025    3206     394 
   Size:        364     495     566    1206     181    2215

  Fragments arranged by size: 

                2215    1206     566     495     364     181
 
BsrGI T'GTAC_A

Cuts at:    672     869     672 
   Size:        197    4830
 
BssSI C'ACGA_G

Cuts at:   3298    4006    3298 
   Size:        708    4319
 
BstAPI GCAn_nnn'nTGC

Cuts at:    607     616    2404     607 
   Size:          9    1788    3230

  Fragments arranged by size: 

                3230    1788       9
 
BstBI TT'CG_AA

Cuts at:   3388    3388 
   Size:       5027
 
BstEII G'GTnAC_C

Cuts at:    115     115 
   Size:       5027
 
BstXI CCAn_nnnn'nTGG

Cuts at:    340     694    1014    1380     340 
   Size:        354     320     366    3987

  Fragments arranged by size: 

                3987     366     354     320
 
BstYI r'GATC_y

Cuts at:      1     335     968    1132    2052    2519    2542    2877    3123 
   Size:        334     633     164     920     467      23     335     246

Cuts at:   3123    3429    3441    3527    4579       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                1052     920     633     467     449     335     334     306
                 246     164      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    560    1582     560 
   Size:       1022    4005
 
BtgI C'CryG_G

Cuts at:    729     816    1385     729 
   Size:         87     569    4371

  Fragments arranged by size: 

                4371     569      87
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2977    3158    3321    2977 
   Size:        181     163    4683

  Fragments arranged by size: 

                4683     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2270    2270 
   Size:       5027
 
ClaI AT'CG_AT

Cuts at:   1601    1601 
   Size:       5027
 
DraI TTT'AAA

Cuts at:   2026    2026 
   Size:       5027
 
DraIII CAC_nnn'GTG

Cuts at:    899    1234     899 
   Size:        335    4692
 
DrdI GACnn_nn'nnGTC

Cuts at:   2733    4077    2733 
   Size:       1344    3683
 
EaeI y'GGCC_r

Cuts at:    819     944    1016    1382    2612    2786    3177    3204    4549 
   Size:        125      72     366    1230     174     391      27    1345

Cuts at:   4549     819 
   Size:       1297

  Fragments arranged by size: 

                1345    1297    1230     391     366     174     125      72
                  27
 
EagI C'GGCC_G

Cuts at:   2612    2612 
   Size:       5027
 
EarI CTCTTCn'nnn_

Cuts at:    293     417     591    1730    1807    3050    3260     293 
   Size:        124     174    1139      77    1243     210    2060

  Fragments arranged by size: 

                2060    1243    1139     210     174     124      77
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3235    3977    4123    4837    3235 
   Size:        742     146     714    3425

  Fragments arranged by size: 

                3425     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    426    1859    2039     426 
   Size:       1433     180    3414

  Fragments arranged by size: 

                3414    1433     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    401     468    1358    1650    2852    3284    3637    4299     401 
   Size:         67     890     292    1202     432     353     662    1129

  Fragments arranged by size: 

                1202    1129     890     662     432     353     292      67
            
 
EcoO109I rG'GnC_Cy

Cuts at:    915    1439    1495    1950    2166     915 
   Size:        524      56     455     216    3776

  Fragments arranged by size: 

                3776     524     455     216      56
 
EcoRI G'AATT_C

Cuts at:    772    2031     772 
   Size:       1259    3768
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3046    3046 
   Size:       5027
 
FspI TGC'GCA

Cuts at:   2808    2808 
   Size:       5027
 
HaeII r_GCGC'y

Cuts at:   2461    2709    3939    4710    2461 
   Size:        248    1230     771    2778

  Fragments arranged by size: 

                2778    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    448     480     689     705     721     737     969    1001    1100 
   Size:         32     209      16      16      16     232      32      99

Cuts at:   1100    1132    2869    2899    2901    2931     448 
   Size:         32    1737      30       2      30    2544

  Fragments arranged by size: 

                2544    1737     232     209      99      32      32      32
                  30      30      16      16      16       2
 
HincII GTy'rAC

Cuts at:    708    1533    2066     708 
   Size:        825     533    3669

  Fragments arranged by size: 

                3669     825     533
 
KasI G'GCGC_C

Cuts at:   2457    2705    2457 
   Size:        248    4779
 
KpnI G_GTAC'C

Cuts at:   2047    2047 
   Size:       5027
 
MluI A'CGCG_T

Cuts at:   4460    4460 
   Size:       5027
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3786    3970    3786 
   Size:        184    4843
 
MscI TGG'CCA

Cuts at:    821    1018    1384    2788     821 
   Size:        197     366    1404    3060

  Fragments arranged by size: 

                3060    1404     366     197
 
MslI CAynn'nnrTG

Cuts at:   1012    1086    1426    3016    3143    1012 
   Size:         74     340    1590     127    2896

  Fragments arranged by size: 

                2896    1590     340     127      74
 
MspA1I CmG'CkG

Cuts at:    421     670    2138    2452    2812    3596    3841     421 
   Size:        249    1468     314     360     784     245    1607

  Fragments arranged by size: 

                1607    1468     784     360     314     249     245
 
NaeI GCC'GGC

Cuts at:    760    3208     760 
   Size:       2448    2579
 
NarI GG'CG_CC

Cuts at:   2458    2706    2458 
   Size:        248    4779
 
NcoI C'CATG_G

Cuts at:    729     816    1385     729 
   Size:         87     569    4371

  Fragments arranged by size: 

                4371     569      87
 
NgoMIV G'CCGG_C

Cuts at:    758    3206     758 
   Size:       2448    2579
 
NspI r_CATG'y

Cuts at:    534    2080    2580    4183     534 
   Size:       1546     500    1603    1378

  Fragments arranged by size: 

                1603    1546    1378     500
 
PasI CC'CwG_GG

Cuts at:    687    1435     687 
   Size:        748    4279
 
PciI A'CATG_T

Cuts at:   4179    4179 
   Size:       5027
 
PflMI CCAn_nnn'nTGG

Cuts at:   1234    1637    1234 
   Size:        403    4624
 
PmeI GTTT'AAAC

Cuts at:   2026    2026 
   Size:       5027
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    136     168    3473    3505     136 
   Size:         32    3305      32    1658

  Fragments arranged by size: 

                3305    1658      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    915     915 
   Size:       5027
 
PspOMI G'GGCC_C

Cuts at:   1439    1439 
   Size:       5027
 
PstI C_TGCA'G

Cuts at:    236    1958    2074    2759     236 
   Size:       1722     116     685    2504

  Fragments arranged by size: 

                2504    1722     685     116
 
PvuI CG_AT'CG

Cuts at:    104    1321     104 
   Size:       1217    3810
 
PvuII CAG'CTG

Cuts at:    421     670    2452    2812     421 
   Size:        249    1782     360    2636

  Fragments arranged by size: 

                2636    1782     360     249
 
RsrII CG'GwC_CG

Cuts at:   3222    3222 
   Size:       5027
 
SacI G_AGCT'C

Cuts at:    428    1861    2041     428 
   Size:       1433     180    3414

  Fragments arranged by size: 

                3414    1433     180
 
SalI G'TCGA_C

Cuts at:   2064    2064 
   Size:       5027
 
SapI GCTCTTCn'nnn_

Cuts at:    417     591    1807    3050    3260     417 
   Size:        174    1216    1243     210    2184

  Fragments arranged by size: 

                2184    1243    1216     210     174
 
SbfI CC_TGCA'GG

Cuts at:   2074    2074 
   Size:       5027
 
ScaI AGT'ACT

Cuts at:   1681    4544    1681 
   Size:       2863    2164
 
SexAI A'CCwGG_T

Cuts at:    281     281 
   Size:       5027
 
SfcI C'TryA_G

Cuts at:     33     232     738    1954    2070    2755    3723    3914      33 
   Size:        199     506    1216     116     685     968     191    1146

  Fragments arranged by size: 

                1216    1146     968     685     506     199     191     116
            
 
SfoI GGC'GCC

Cuts at:   2459    2707    2459 
   Size:        248    4779
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5027
 
SmaI CCC'GGG

Cuts at:   2049    2049 
   Size:       5027
 
SmlI C'TyrA_G

Cuts at:   2182    3097    3811    4073    2182 
   Size:        915     714     262    3136

  Fragments arranged by size: 

                3136     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5027
 
SphI G_CATG'C

Cuts at:   2080    2580    2080 
   Size:        500    4527
 
StuI AGG'CCT

Cuts at:    209     209 
   Size:       5027
 
StyI C'CwwG_G

Cuts at:    729     816     827     973    1385    2006    2161    2515     729 
   Size:         87      11     146     412     621     155     354    3241

  Fragments arranged by size: 

                3241     621     412     354     155     146      87      11
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1714    2610    2743    3202    3411    1714 
   Size:        896     133     459     209    3330

  Fragments arranged by size: 

                3330     896     459     209     133
 
TatI w'GTAC_w

Cuts at:    672     869    1679    4542     672 
   Size:        197     810    2863    1157

  Fragments arranged by size: 

                2863    1157     810     197
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1782    1814    4544    4576    1782 
   Size:         32    2730      32    2233

  Fragments arranged by size: 

                2730    2233      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2824    2824 
   Size:       5027
 
XbaI T'CTAG_A

Cuts at:   2058    2058 
   Size:       5027
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2522    2522 
   Size:       5027
 
XmaI C'CCGG_G

Cuts at:   2047    2047 
   Size:       5027
 
XmnI GAAnn'nnTTC

Cuts at:    384     384 
   Size:       5027
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AleI     AscI     BaeI    BfrBI
     BmtI    BsiWI     BsmI    BsmBI    BspEI   BssHII  BstZ17I    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com