Restriction map for pF1KA0571
 (Linear) (Six-base) MAPSORT of: KIAA0571.seq  Check: 340  from: 1  to: 1914



KIAA0571.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1279    1914 
   Size:       1279     635
 
AccI GT'mk_AC

Cuts at:      0     818    1310    1914 
   Size:        818     492     604

  Fragments arranged by size: 

                 818     604     492
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1250    1914 
   Size:       1250     664
 
AhdI GACnn_n'nnGTC

Cuts at:      0     595    1914 
   Size:        595    1319
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     110    1914 
   Size:        110    1804
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28      60    1914 
   Size:         28      32    1854

  Fragments arranged by size: 

                1854      32      28
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1660    1845    1914 
   Size:       1660     185      69

  Fragments arranged by size: 

                1660     185      69
 
ApaI G_GGCC'C

Cuts at:      0    1335    1914 
   Size:       1335     579
 
ApaLI G'TGCA_C

Cuts at:      0    1378    1914 
   Size:       1378     536
 
ApoI r'AATT_y

Cuts at:      0     664    1914 
   Size:        664    1250
 
AvrII C'CTAG_G

Cuts at:      0     865    1914 
   Size:        865    1049
 
BanI G'GyrC_C

Cuts at:      0     526    1903    1914 
   Size:        526    1377      11

  Fragments arranged by size: 

                1377     526      11
 
BanII G_rGCy'C

Cuts at:      0     320    1327    1335    1753    1914 
   Size:        320    1007       8     418     161

  Fragments arranged by size: 

                1007     418     320     161       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1238    1840    1914 
   Size:       1238     602      74

  Fragments arranged by size: 

                1238     602      74
 
BbvCI CC'TCA_GC

Cuts at:      0     248     282     360     992    1914 
   Size:        248      34      78     632     922

  Fragments arranged by size: 

                 922     632     248      78      34
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     783     817    1011    1045    1914 
   Size:        783      34     194      34     869

  Fragments arranged by size: 

                 869     783     194      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     651     734    1914 
   Size:        651      83    1180

  Fragments arranged by size: 

                1180     651      83
 
BglII A'GATC_T

Cuts at:      0     227    1024    1914 
   Size:        227     797     890

  Fragments arranged by size: 

                 890     797     227
 
BlpI GC'TnA_GC

Cuts at:      0     314     319    1914 
   Size:        314       5    1595

  Fragments arranged by size: 

                1595     314       5
 
Bme1580I G_kGCm'C

Cuts at:      0     529    1247    1335    1382    1914 
   Size:        529     718      88      47     532

  Fragments arranged by size: 

                 718     532     529      88      47
 
BmgBI CAC'GTC

Cuts at:      0    1875    1914 
   Size:       1875      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1563    1648    1670    1914 
   Size:       1563      85      22     244

  Fragments arranged by size: 

                1563     244      85      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     360    1542    1914 
   Size:        293      67    1182     372

  Fragments arranged by size: 

                1182     372     293      67
 
Bpu10I CC'TnA_GC

Cuts at:      0     248     282     360     992    1914 
   Size:        248      34      78     632     922

  Fragments arranged by size: 

                 922     632     248      78      34
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     993    1099    1914 
   Size:        993     106     815

  Fragments arranged by size: 

                 993     815     106
 
BsaHI Gr'CG_yC

Cuts at:      0     775    1914 
   Size:        775    1139
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58    1546    1576    1914 
   Size:         28      30    1488      30     338

  Fragments arranged by size: 

                1488     338      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265     564    1554    1914 
   Size:        265     299     990     360

  Fragments arranged by size: 

                 990     360     299     265
 
BseYI C'CCAG_C

Cuts at:      0     778    1390    1914 
   Size:        778     612     524

  Fragments arranged by size: 

                 778     612     524
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     813    1160    1914 
   Size:        813     347     754

  Fragments arranged by size: 

                 813     754     347
 
BsiEI CG_ry'CG

Cuts at:      0    1213    1914 
   Size:       1213     701
 
BsiHKAI G_wGCw'C

Cuts at:      0     259     320    1382    1711    1753    1914 
   Size:        259      61    1062     329      42     161

  Fragments arranged by size: 

                1062     329     259     161      61      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     259     320     529    1247    1327    1335    1382    1711 
   Size:        259      61     209     718      80       8      47     329

Cuts at:   1711    1753    1914 
   Size:         42     161

  Fragments arranged by size: 

                 718     329     259     209     161      80      61      47
                  42       8
 
BspHI T'CATG_A

Cuts at:      0     545    1914 
   Size:        545    1369
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78    1279    1914 
   Size:         78    1201     635

  Fragments arranged by size: 

                1201     635      78
 
BsrBI CCG'CTC

Cuts at:      0       5     302    1914 
   Size:          5     297    1612

  Fragments arranged by size: 

                1612     297       5
 
BsrDI GCAATG_nn'

Cuts at:      0     591    1073    1914 
   Size:        591     482     841

  Fragments arranged by size: 

                 841     591     482
 
BsrFI r'CCGG_y

Cuts at:      0     286     650    1145    1711    1914 
   Size:        286     364     495     566     203

  Fragments arranged by size: 

                 566     495     364     286     203
 
BsrGI T'GTAC_A

Cuts at:      0     564     761    1914 
   Size:        564     197    1153

  Fragments arranged by size: 

                1153     564     197
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     499     508    1914 
   Size:        499       9    1406

  Fragments arranged by size: 

                1406     499       9
 
BstEII G'GTnAC_C

Cuts at:      0       7    1914 
   Size:          7    1907
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     232     586     906    1272    1914 
   Size:        232     354     320     366     642

  Fragments arranged by size: 

                 642     366     354     320     232
 
BstYI r'GATC_y

Cuts at:      0     227     860    1024    1914 
   Size:        227     633     164     890

  Fragments arranged by size: 

                 890     633     227     164
 
Bsu36I CC'TnA_GG

Cuts at:      0     452    1474    1914 
   Size:        452    1022     440

  Fragments arranged by size: 

                1022     452     440
 
BtgI C'CryG_G

Cuts at:      0     621     708    1277    1914 
   Size:        621      87     569     637

  Fragments arranged by size: 

                 637     621     569      87
 
ClaI AT'CG_AT

Cuts at:      0    1493    1914 
   Size:       1493     421
 
DraIII CAC_nnn'GTG

Cuts at:      0     791    1126    1914 
   Size:        791     335     788

  Fragments arranged by size: 

                 791     788     335
 
EaeI y'GGCC_r

Cuts at:      0     711     836     908    1274    1914 
   Size:        711     125      72     366     640

  Fragments arranged by size: 

                 711     640     366     125      72
 
EarI CTCTTCn'nnn_

Cuts at:      0     185     309     483    1622    1699    1914 
   Size:        185     124     174    1139      77     215

  Fragments arranged by size: 

                1139     215     185     174     124      77
 
EcoICRI GAG'CTC

Cuts at:      0     318    1751    1914 
   Size:        318    1433     163

  Fragments arranged by size: 

                1433     318     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     360    1250    1542    1914 
   Size:        293      67     890     292     372

  Fragments arranged by size: 

                 890     372     293     292      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0     807    1331    1387    1842    1914 
   Size:        807     524      56     455      72

  Fragments arranged by size: 

                 807     524     455      72      56
 
EcoRI G'AATT_C

Cuts at:      0     664    1914 
   Size:        664    1250
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372     581     597     613     629     861     893 
   Size:        340      32     209      16      16      16     232      32

Cuts at:    893     992    1024    1914 
   Size:         99      32     890

  Fragments arranged by size: 

                 890     340     232     209      99      32      32      32
                  16      16      16
 
HincII GTy'rAC

Cuts at:      0     600    1425    1914 
   Size:        600     825     489

  Fragments arranged by size: 

                 825     600     489
 
MscI TGG'CCA

Cuts at:      0     713     910    1276    1914 
   Size:        713     197     366     638

  Fragments arranged by size: 

                 713     638     366     197
 
MslI CAynn'nnrTG

Cuts at:      0     904     978    1318    1914 
   Size:        904      74     340     596

  Fragments arranged by size: 

                 904     596     340      74
 
MspA1I CmG'CkG

Cuts at:      0     313     562    1914 
   Size:        313     249    1352

  Fragments arranged by size: 

                1352     313     249
 
NaeI GCC'GGC

Cuts at:      0     652    1914 
   Size:        652    1262
 
NcoI C'CATG_G

Cuts at:      0     621     708    1277    1914 
   Size:        621      87     569     637

  Fragments arranged by size: 

                 637     621     569      87
 
NgoMIV G'CCGG_C

Cuts at:      0     650    1914 
   Size:        650    1264
 
NspI r_CATG'y

Cuts at:      0     426    1914 
   Size:        426    1488
 
PasI CC'CwG_GG

Cuts at:      0     579    1327    1914 
   Size:        579     748     587

  Fragments arranged by size: 

                 748     587     579
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1126    1529    1914 
   Size:       1126     403     385

  Fragments arranged by size: 

                1126     403     385
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60    1914 
   Size:         28      32    1854

  Fragments arranged by size: 

                1854      32      28
 
PpuMI rG'GwC_Cy

Cuts at:      0     807    1914 
   Size:        807    1107
 
PspOMI G'GGCC_C

Cuts at:      0    1331    1914 
   Size:       1331     583
 
PstI C_TGCA'G

Cuts at:      0     128    1850    1914 
   Size:        128    1722      64

  Fragments arranged by size: 

                1722     128      64
 
PvuI CG_AT'CG

Cuts at:      0    1213    1914 
   Size:       1213     701
 
PvuII CAG'CTG

Cuts at:      0     313     562    1914 
   Size:        313     249    1352

  Fragments arranged by size: 

                1352     313     249
 
SacI G_AGCT'C

Cuts at:      0     320    1753    1914 
   Size:        320    1433     161

  Fragments arranged by size: 

                1433     320     161
 
SapI GCTCTTCn'nnn_

Cuts at:      0     309     483    1699    1914 
   Size:        309     174    1216     215

  Fragments arranged by size: 

                1216     309     215     174
 
ScaI AGT'ACT

Cuts at:      0    1573    1914 
   Size:       1573     341
 
SexAI A'CCwGG_T

Cuts at:      0     173    1914 
   Size:        173    1741
 
SfcI C'TryA_G

Cuts at:      0     124     630    1846    1914 
   Size:        124     506    1216      68

  Fragments arranged by size: 

                1216     506     124      68
 
StuI AGG'CCT

Cuts at:      0     101    1914 
   Size:        101    1813
 
StyI C'CwwG_G

Cuts at:      0     621     708     719     865    1277    1898    1914 
   Size:        621      87      11     146     412     621      16

  Fragments arranged by size: 

                 621     621     412     146      87      16      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1606    1914 
   Size:       1606     308
 
TatI w'GTAC_w

Cuts at:      0     564     761    1571    1914 
   Size:        564     197     810     343

  Fragments arranged by size: 

                 810     564     343     197
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1674    1706    1914 
   Size:       1674      32     208

  Fragments arranged by size: 

                1674     208      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     276    1914 
   Size:        276    1638
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI    AvrII     BanI    BanII     BbsI    BbvCI     BcgI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     ClaI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI
     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII     SacI     SapI
     ScaI    SexAI     SfcI     StuI     StyI    TaqII     TatI     TstI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BbeI    BciVI
     BclI    BfrBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI
    BsiWI     BsmI    BsmBI    BspEI   BssHII    BssSI    BstBI  BstZ17I
    BtgZI     BtsI    CspCI     DraI     DrdI     EagI     EciI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI    RsrII    SacII     SalI    SanDI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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