Restriction map for pF1KA0567
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BlnI        BlpI        
Bpu1102I    BsaHI       BsiEI       BsiWI       BsmFI       
Bsp1407I    BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstEII      
BstPI       CelII       Cfr10I      CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoT14I     EcoT22I     FseI        
HaeII       HgaI        Hin1I       HpaI        KasI        
KpnI        KspI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfoI        
SgrAI       SmaI        Sse8387I    SspBI       StuI        
StyI        SwaI        Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII        2794  CGCG                 CG^CG
AflII         778  CTTAAG               C^TTAAG
AocI          551  CCTCAGG              CC^T[ACGT]AGG
AseI         1207  ATTAAT               AT^TAAT
AsnI         1207  ATTAAT               AT^TAAT
AspHI        2410  GAGCAC               G[AT]GC[AT]^C
AviII        1920  TGCGCA               TGC^GCA
BalI          265  TGGCCA               TGG^CCA
BclI          357  TGATCA               T^GATCA
BfrI          778  CTTAAG               C^TTAAG
BglI           19  GCCGCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1062  AGATCT               A^GATCT
BmyI         2410  GAGCAC               G[AGT]GC[ACT]^C
BpmI          974  CTCCAG               CTGGAG(16/14)
BsaAI         209  TACGTA               [CT]AC^GT[GA]
BsaBI        2198  GATCCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          667  CTGCAC               GTGCAG(16/14)
BsiHKAI      2410  GAGCAC               G[AT]GC[AT]^C
BsmBI        2263  CGTCTC               CGTCTC(1/5)
Bsp1286I     2410  GAGCAC               G[AGT]GC[ACT]^C
BspDI         439  ATCGAT               AT^CGAT
BspLU11I      797  ACATGT               A^CATGT
Bst1107I     2645  GTATAC               GTA^TAC
BstBI        1094  TTCGAA               TT^CGAA
BstUI        2794  CGCG                 CG^CG
BstXI        1837  CCAAAACATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2645  GTATAC               GTA^TAC
Bsu36I        551  CCTCAGG              CC^T[ACGT]AGG
ClaI          439  ATCGAT               AT^CGAT
DsaI          865  CCACGG               C^C[GA][CT]GG
EaeI          265  TGGCCA               [CT]^GGCC[GA]
Eco81I        551  CCTCAGG              CC^T[ACGT]AGG
EcoRV        1960  GATATC               GAT^ATC
FbaI          357  TGATCA               T^GATCA
FspI         1920  TGCGCA               TGC^GCA
HincII       1189  GTTGAC               GT[CT]^[GA]AC
HindII       1189  GTTGAC               GT[CT]^[GA]AC
MamI         2198  GATCCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2285  CAATTG               C^AATTG
MluI         2793  ACGCGT               A^CGCGT
MluNI         265  TGGCCA               TGG^CCA
MscI          265  TGGCCA               TGG^CCA
MslI         1950  CACCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         2285  CAATTG               C^AATTG
MvnI         2794  CGCG                 CG^CG
NspV         1094  TTCGAA               TT^CGAA
PpuMI         999  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1207  ATTAAT               AT^TAAT
PstI         2254  CTGCAG               CTGCA^G
PvuII        1916  CAGCTG               CAG^CTG
SexAI        1197  ACCAGGT              A^CC[AT]GGT
SfiI           18  GGCCGCTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1094  TTCGAA               TT^CGAA
SnaBI         209  TACGTA               TAC^GTA
SpeI          904  ACTAGT               A^CTAGT
SspI          936  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI         1405  GTAGAC               GT^[AC][GT]AC
             2645  GTATAC              
AccIII        561  TCCGGA               T^CCGGA
             2450  TCCGGA              
AfaI         1124  GTAC                 GT^AC
             1666  GTAC                
AspI            8  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1348  GACTTGGTC           
AvaI         1034  CTCGGG               C^[CT]CG[GA]G
             2468  CCCGAG              
BciVI        2271  GGATAC               GTATCC(6/5)
             2502  GGATAC              
BfaI          313  CTAG                 C^TAG
              905  CTAG                
BsaI         1138  GAGACC               GGTCTC(1/5)
             2023  GAGACC              
BseAI         561  TCCGGA               T^CCGGA
             2450  TCCGGA              
BseRI        2407  GAGGAG               GAGGAG(10/8)
             2471  GAGGAG              
BsmI         2617  GAATGC               GAATGC(1/-1)
              761  GCATTC              
BsoBI        1034  CTCGGG               C^[CT]CG[GA]G
             2468  CCCGAG              
BspEI         561  TCCGGA               T^CCGGA
             2450  TCCGGA              
BsrDI        2217  GCAATG               GCAATG(2/0)
             2621  GCAATG              
CfoI         1921  GCGC                 GCG^C
             2652  GCGC                
DraI         1021  TTTAAA               TTT^AAA
             2535  TTTAAA              
DraII         999  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1161  AGGCCCT             
DraIII       1006  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1609  CACCAAGTG           
EcoO109I      999  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1161  AGGCCCT             
EcoRI        1572  GAATTC               G^AATTC
             1932  GAATTC              
FauI         2045  CCCGC                CCCGC(4/6)
             1755  GCGGG               
HhaI         1921  GCGC                 GCG^C
             2652  GCGC                
HinP1I       1921  GCGC                 G^CGC
             2652  GCGC                
MaeI          313  CTAG                 C^TAG
              905  CTAG                
MroI          561  TCCGGA               T^CCGGA
             2450  TCCGGA              
MspA1I         20  CCGCTG               C[AC]G^C[GT]G
             1916  CAGCTG              
PflFI           8  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1348  GACTTGGTC           
RsaI         1124  GTAC                 GT^AC
             1666  GTAC                
SapI         1467  GCTCTTC              GCTCTTC(1/4)
             2863  GCTCTTC             
SfcI         1171  CTACAG               C^T[GA][CT]AG
             2254  CTGCAG              
SphI         1595  GCATGC               GCATG^C
             2654  GCATGC              
Tth111I         8  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1348  GACTTGGTC           
XcmI         1400  CCAAAGTAGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2372  CCAAGAATGAATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AflIII      AluI        AlwI        
ApoI        Asp700I     AvaII       BbsI        BbvI        
BpuAI       BsaJI       BsaWI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com