Restriction map for pF1KA0567
 (Linear) (Six-base) MAPSORT of: KIAA0567.seq  Check: 7534  from: 1  to: 2880



KIAA0567.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AccI GT'mk_AC

Cuts at:      0    1406    2646    2880 
   Size:       1406    1240     234

  Fragments arranged by size: 

                1406    1240     234
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637     827    2723    2774    2880 
   Size:        637     190    1896      51     106

  Fragments arranged by size: 

                1896     637     190     106      51
 
AflII C'TTAA_G

Cuts at:      0     778    2880 
   Size:        778    2102
 
AflIII A'CryG_T

Cuts at:      0     797     967    1708    2793    2880 
   Size:        797     170     741    1085      87

  Fragments arranged by size: 

                1085     797     741     170      87
 
AleI CACnn'nnGTG

Cuts at:      0    1954    2880 
   Size:       1954     926
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2240    2880 
   Size:       2240     640
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2449    2481    2880 
   Size:       2449      32     399

  Fragments arranged by size: 

                2449     399      32
 
ApoI r'AATT_y

Cuts at:      0     220     258    1572    1626    1932    2038    2831    2880 
   Size:        220      38    1314      54     306     106     793      49

  Fragments arranged by size: 

                1314     793     306     220     106      54      49      38
            
 
AseI AT'TA_AT

Cuts at:      0    1208    2880 
   Size:       1208    1672
 
AvaI C'yCGr_G

Cuts at:      0    1034    2468    2880 
   Size:       1034    1434     412

  Fragments arranged by size: 

                1434    1034     412
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1657    1690    2880 
   Size:       1657      33    1190

  Fragments arranged by size: 

                1657    1190      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     485    2198    2532    2816    2880 
   Size:        485    1713     334     284      64

  Fragments arranged by size: 

                1713     485     334     284      64
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2265    2496    2880 
   Size:       2265     231     384

  Fragments arranged by size: 

                2265     384     231
 
BclI T'GATC_A

Cuts at:      0     357    2880 
   Size:        357    2523
 
BglI GCCn_nnn'nGGC

Cuts at:      0      25    2880 
   Size:         25    2855
 
BglII A'GATC_T

Cuts at:      0    1062    2880 
   Size:       1062    1818
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     959    2880 
   Size:        959    1921
 
Bpu10I CC'TnA_GC

Cuts at:      0     526    2880 
   Size:        526    2354
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2184    2251    2800    2880 
   Size:       2184      67     549      80

  Fragments arranged by size: 

                2184     549      80      67
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1132    2017    2880 
   Size:       1132     885     863

  Fragments arranged by size: 

                1132     885     863
 
BsaAI yAC'GTr

Cuts at:      0     211    2880 
   Size:        211    2669
 
BsaBI GATnn'nnATC

Cuts at:      0    2202    2880 
   Size:       2202     678
 
BsaWI w'CCGG_w

Cuts at:      0     378     561    1766    2450    2880 
   Size:        378     183    1205     684     430

  Fragments arranged by size: 

                1205     684     430     378     183
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     957     987    2377    2407    2465    2495    2880 
   Size:        957      30    1390      30      58      30     385

  Fragments arranged by size: 

                1390     957     385      58      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2422    2486    2880 
   Size:       2422      64     394

  Fragments arranged by size: 

                2422     394      64
 
BseYI C'CCAG_C

Cuts at:      0    2415    2880 
   Size:       2415     465
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652    2880 
   Size:        652    2228
 
BsiHKAI G_wGCw'C

Cuts at:      0    2414    2880 
   Size:       2414     466
 
BsmI GAATG_Cn'

Cuts at:      0     761    2623    2880 
   Size:        761    1862     257

  Fragments arranged by size: 

                1862     761     257
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2269    2880 
   Size:       2269     611
 
Bsp1286I G_dGCh'C

Cuts at:      0    2414    2880 
   Size:       2414     466
 
BspEI T'CCGG_A

Cuts at:      0     561    2450    2880 
   Size:        561    1889     430

  Fragments arranged by size: 

                1889     561     430
 
BsrDI GCAATG_nn'

Cuts at:      0    2224    2628    2880 
   Size:       2224     404     252

  Fragments arranged by size: 

                2224     404     252
 
BstBI TT'CG_AA

Cuts at:      0    1095    2880 
   Size:       1095    1785
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1844    2880 
   Size:       1844    1036
 
BstYI r'GATC_y

Cuts at:      0     595     949    1062    1315    2484    2880 
   Size:        595     354     113     253    1169     396

  Fragments arranged by size: 

                1169     595     396     354     253     113
 
BstZ17I GTA'TAC

Cuts at:      0    2647    2880 
   Size:       2647     233
 
Bsu36I CC'TnA_GG

Cuts at:      0     552    2880 
   Size:        552    2328
 
BtgI C'CryG_G

Cuts at:      0     865    2880 
   Size:        865    2015
 
ClaI AT'CG_AT

Cuts at:      0     440    2880 
   Size:        440    2440
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1852    1887    2880 
   Size:       1852      35     993

  Fragments arranged by size: 

                1852     993      35
 
DraI TTT'AAA

Cuts at:      0    1023    2537    2880 
   Size:       1023    1514     343

  Fragments arranged by size: 

                1514    1023     343
 
DraIII CAC_nnn'GTG

Cuts at:      0    1011    1614    2880 
   Size:       1011     603    1266

  Fragments arranged by size: 

                1266    1011     603
 
EaeI y'GGCC_r

Cuts at:      0     265    2880 
   Size:        265    2615
 
EarI CTCTTCn'nnn_

Cuts at:      0     192    1474    1553    2240    2565    2870    2880 
   Size:        192    1282      79     687     325     305      10

  Fragments arranged by size: 

                1282     687     325     305     192      79      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2821    2880 
   Size:       2821      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637     827     959    2723    2774    2880 
   Size:        637     190     132    1764      51     106

  Fragments arranged by size: 

                1764     637     190     132     106      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1000    1162    2880 
   Size:       1000     162    1718

  Fragments arranged by size: 

                1718    1000     162
 
EcoRI G'AATT_C

Cuts at:      0    1572    1932    2880 
   Size:       1572     360     948

  Fragments arranged by size: 

                1572     948     360
 
EcoRV GAT'ATC

Cuts at:      0    1962    2880 
   Size:       1962     918
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1081    2860    2880 
   Size:       1081    1779      20

  Fragments arranged by size: 

                1779    1081      20
 
FspI TGC'GCA

Cuts at:      0    1922    2880 
   Size:       1922     958
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     342     374     580     612     808     840     957     984 
   Size:        342      32     206      32     196      32     117      27

Cuts at:    984     989    1016    1129    1161    2880 
   Size:          5      27     113      32    1719

  Fragments arranged by size: 

                1719     342     206     196     117     113      32      32
                  32      32      27      27       5
 
HincII GTy'rAC

Cuts at:      0    1191    2880 
   Size:       1191    1689
 
HindIII A'AGCT_T

Cuts at:      0     145     775    1268    1481    1966    2490    2880 
   Size:        145     630     493     213     485     524     390

  Fragments arranged by size: 

                 630     524     493     485     390     213     145
 
MfeI C'AATT_G

Cuts at:      0    2285    2880 
   Size:       2285     595
 
MluI A'CGCG_T

Cuts at:      0    2793    2880 
   Size:       2793      87
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     861     893    2594    2880 
   Size:        861      32    1701     286

  Fragments arranged by size: 

                1701     861     286      32
 
MscI TGG'CCA

Cuts at:      0     267    2880 
   Size:        267    2613
 
MslI CAynn'nnrTG

Cuts at:      0    1954    2880 
   Size:       1954     926
 
MspA1I CmG'CkG

Cuts at:      0      22    1918    2880 
   Size:         22    1896     962

  Fragments arranged by size: 

                1896     962      22
 
NspI r_CATG'y

Cuts at:      0     801    1278    1599    2658    2880 
   Size:        801     477     321    1059     222

  Fragments arranged by size: 

                1059     801     477     321     222
 
PciI A'CATG_T

Cuts at:      0     797    2880 
   Size:        797    2083
 
PpuMI rG'GwC_Cy

Cuts at:      0    1000    2880 
   Size:       1000    1880
 
PsiI TTA'TAA

Cuts at:      0     845    2880 
   Size:        845    2035
 
PstI C_TGCA'G

Cuts at:      0    2258    2880 
   Size:       2258     622
 
PvuII CAG'CTG

Cuts at:      0    1918    2880 
   Size:       1918     962
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1474    2870    2880 
   Size:       1474    1396      10

  Fragments arranged by size: 

                1474    1396      10
 
SexAI A'CCwGG_T

Cuts at:      0    1197    2880 
   Size:       1197    1683
 
SfcI C'TryA_G

Cuts at:      0    1171    2254    2880 
   Size:       1171    1083     626

  Fragments arranged by size: 

                1171    1083     626
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      25    2880 
   Size:         25    2855
 
SmlI C'TyrA_G

Cuts at:      0     778    2163    2266    2815    2880 
   Size:        778    1385     103     549      65

  Fragments arranged by size: 

                1385     778     549     103      65
 
SnaBI TAC'GTA

Cuts at:      0     211    2880 
   Size:        211    2669
 
SpeI A'CTAG_T

Cuts at:      0     904    2880 
   Size:        904    1976
 
SphI G_CATG'C

Cuts at:      0    1599    2658    2880 
   Size:       1599    1059     222

  Fragments arranged by size: 

                1599    1059     222
 
SspI AAT'ATT

Cuts at:      0     938    2880 
   Size:        938    1942
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2210    2429    2880 
   Size:       2210     219     451

  Fragments arranged by size: 

                2210     451     219
 
Tth111I GACn'n_nGTC

Cuts at:      0      11    1351    2880 
   Size:         11    1340    1529

  Fragments arranged by size: 

                1529    1340      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1407    2379    2880 
   Size:       1407     972     501

  Fragments arranged by size: 

                1407     972     501
 
XmnI GAAnn'nnTTC

Cuts at:      0     662    1856    2864    2880 
   Size:        662    1194    1008      16

  Fragments arranged by size: 

                1194    1008     662      16
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AleI     AlfI     AloI     ApoI
     AseI     AvaI     BaeI     BbsI    BciVI     BclI     BglI    BglII
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BsrDI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    Hin4I   HincII  HindIII     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NspI     PciI    PpuMI
     PsiI     PstI    PvuII     SapI    SexAI     SfcI     SfiI     SmlI
    SnaBI     SpeI     SphI     SspI    TaqII  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI    AlwNI
     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BfrBI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BsaHI    BsiEI    BsiWI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BtgZI     BtsI
     DrdI     EagI  EcoICRI    EcoNI     FseI    FspAI    HaeII     HpaI
     KasI     KpnI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfoI     SgfI
    SgrAI     SmaI     SrfI     StuI     StyI     SwaI     TatI     TstI
     XbaI     XhoI     XmaI     ZraI
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