Restriction map for pF1KA0473
 (Linear) (Six-base) MAPSORT of: KIAA0473.seq  Check: 4679  from: 1  to: 2739



KIAA0473.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2580    2739 
   Size:       2580     159
 
AccI GT'mk_AC

Cuts at:      0     323    1663    2739 
   Size:        323    1340    1076

  Fragments arranged by size: 

                1340    1076     323
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     952    2739 
   Size:        952    1787
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1421    2192    2739 
   Size:       1421     771     547

  Fragments arranged by size: 

                1421     771     547
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2025    2739 
   Size:       2025     714
 
ApaLI G'TGCA_C

Cuts at:      0    2626    2739 
   Size:       2626     113
 
ApoI r'AATT_y

Cuts at:      0    2707    2739 
   Size:       2707      32
 
BamHI G'GATC_C

Cuts at:      0    1981    2028    2445    2739 
   Size:       1981      47     417     294

  Fragments arranged by size: 

                1981     417     294      47
 
BanI G'GyrC_C

Cuts at:      0      22    1732    2176    2739 
   Size:         22    1710     444     563

  Fragments arranged by size: 

                1710     563     444      22
 
BanII G_rGCy'C

Cuts at:      0      47    2505    2690    2739 
   Size:         47    2458     185      49

  Fragments arranged by size: 

                2458     185      49      47
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1334    2354    2739 
   Size:       1334    1020     385

  Fragments arranged by size: 

                1334    1020     385
 
BbvCI CC'TCA_GC

Cuts at:      0    1061    2210    2739 
   Size:       1061    1149     529

  Fragments arranged by size: 

                1149    1061     529
 
BfrBI ATG'CAT

Cuts at:      0    1036    2520    2739 
   Size:       1036    1484     219

  Fragments arranged by size: 

                1484    1036     219
 
BglI GCCn_nnn'nGGC

Cuts at:      0     396    2739 
   Size:        396    2343
 
Bme1580I G_kGCm'C

Cuts at:      0    2630    2739 
   Size:       2630     109
 
BmgBI CAC'GTC

Cuts at:      0     885    2739 
   Size:        885    1854
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     401    1662    2654    2739 
   Size:        401    1261     992      85

  Fragments arranged by size: 

                1261     992     401      85
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     195    1223    1816    1928    2739 
   Size:         17     178    1028     593     112     811

  Fragments arranged by size: 

                1028     811     593     178     112      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     938    1061    2204    2210    2739 
   Size:        938     123    1143       6     529

  Fragments arranged by size: 

                1143     938     529     123       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     927    2739 
   Size:        927    1812
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      67     378    2536    2739 
   Size:         67     311    2158     203

  Fragments arranged by size: 

                2158     311     203      67
 
BsaBI GATnn'nnATC

Cuts at:      0    1157    2739 
   Size:       1157    1582
 
BsaHI Gr'CG_yC

Cuts at:      0    2137    2739 
   Size:       2137     602
 
BsaWI w'CCGG_w

Cuts at:      0    1978    2739 
   Size:       1978     761
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     872     902    2533    2563    2739 
   Size:        872      30    1631      30     176

  Fragments arranged by size: 

                1631     872     176      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1538    2220    2739 
   Size:       1538     682     519

  Fragments arranged by size: 

                1538     682     519
 
BseYI C'CCAG_C

Cuts at:      0      46     567    1162    1441    1785    2144    2215    2536 
   Size:         46     521     595     279     344     359      71     321

Cuts at:   2536    2739 
   Size:        203

  Fragments arranged by size: 

                 595     521     359     344     321     279     203      71
                  46
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     397    1128    1616    2739 
   Size:        107     290     731     488    1123

  Fragments arranged by size: 

                1123     731     488     290     107
 
BsiHKAI G_wGCw'C

Cuts at:      0    1758    2630    2690    2739 
   Size:       1758     872      60      49

  Fragments arranged by size: 

                1758     872      60      49
 
BsmI GAATG_Cn'

Cuts at:      0     385    2739 
   Size:        385    2354
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     875    1712    2144    2739 
   Size:        875     837     432     595

  Fragments arranged by size: 

                 875     837     595     432
 
Bsp1286I G_dGCh'C

Cuts at:      0      47    1758    2505    2630    2690    2739 
   Size:         47    1711     747     125      60      49

  Fragments arranged by size: 

                1711     747     125      60      49      47
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1665    2580    2739 
   Size:       1665     915     159

  Fragments arranged by size: 

                1665     915     159
 
BsrBI CCG'CTC

Cuts at:      0    1685    2739 
   Size:       1685    1054
 
BsrDI GCAATG_nn'

Cuts at:      0    1410    1523    2739 
   Size:       1410     113    1216

  Fragments arranged by size: 

                1410    1216     113
 
BsrFI r'CCGG_y

Cuts at:      0     926    2739 
   Size:        926    1813
 
BsrGI T'GTAC_A

Cuts at:      0    2603    2739 
   Size:       2603     136
 
BstBI TT'CG_AA

Cuts at:      0     282    2739 
   Size:        282    2457
 
BstEII G'GTnAC_C

Cuts at:      0     942    2739 
   Size:        942    1797
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2442    2739 
   Size:       2442     297
 
BstYI r'GATC_y

Cuts at:      0    1981    2028    2445    2739 
   Size:       1981      47     417     294

  Fragments arranged by size: 

                1981     417     294      47
 
BstZ17I GTA'TAC

Cuts at:      0     324    2739 
   Size:        324    2415
 
Bsu36I CC'TnA_GG

Cuts at:      0    1209    1481    2739 
   Size:       1209     272    1258

  Fragments arranged by size: 

                1258    1209     272
 
BtgI C'CryG_G

Cuts at:      0    1129    2739 
   Size:       1129    1610
 
BtsI GCAGTG_nn'

Cuts at:      0     486    2739 
   Size:        486    2253
 
DraI TTT'AAA

Cuts at:      0    1010    2739 
   Size:       1010    1729
 
EaeI y'GGCC_r

Cuts at:      0    2432    2739 
   Size:       2432     307
 
EarI CTCTTCn'nnn_

Cuts at:      0    1177    1182    2739 
   Size:       1177       5    1557

  Fragments arranged by size: 

                1557    1177       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1526    2739 
   Size:       1526    1213
 
EcoICRI GAG'CTC

Cuts at:      0    2688    2739 
   Size:       2688      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     195     952    1223    1816    1928    2739 
   Size:         17     178     757     271     593     112     811

  Fragments arranged by size: 

                 811     757     593     271     178     112      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2395    2739 
   Size:       2395     344
 
EcoRV GAT'ATC

Cuts at:      0    1233    2739 
   Size:       1233    1506
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135    1865    2739 
   Size:        135    1730     874

  Fragments arranged by size: 

                1730     874     135
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1288    1320    2013    2045    2341    2373    2686    2718 
   Size:       1288      32     693      32     296      32     313      32

Cuts at:   2718    2739 
   Size:         21

  Fragments arranged by size: 

                1288     693     313     296      32      32      32      32
                  21
 
HincII GTy'rAC

Cuts at:      0     249     914    1673    2739 
   Size:        249     665     759    1066

  Fragments arranged by size: 

                1066     759     665     249
 
HindIII A'AGCT_T

Cuts at:      0     285    2739 
   Size:        285    2454
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1735    2739 
   Size:        415    1320    1004

  Fragments arranged by size: 

                1320    1004     415
 
MscI TGG'CCA

Cuts at:      0    2434    2739 
   Size:       2434     305
 
MslI CAynn'nnrTG

Cuts at:      0     871    1403    1649    2739 
   Size:        871     532     246    1090

  Fragments arranged by size: 

                1090     871     532     246
 
MspA1I CmG'CkG

Cuts at:      0    1543    1600    1963    2338    2739 
   Size:       1543      57     363     375     401

  Fragments arranged by size: 

                1543     401     375     363      57
 
NaeI GCC'GGC

Cuts at:      0     928    2739 
   Size:        928    1811
 
NcoI C'CATG_G

Cuts at:      0    1129    2739 
   Size:       1129    1610
 
NgoMIV G'CCGG_C

Cuts at:      0     926    2739 
   Size:        926    1813
 
NsiI A_TGCA'T

Cuts at:      0    1038    2522    2739 
   Size:       1038    1484     217

  Fragments arranged by size: 

                1484    1038     217
 
NspI r_CATG'y

Cuts at:      0    1040    1867    1927    2223    2739 
   Size:       1040     827      60     296     516

  Fragments arranged by size: 

                1040     827     516     296      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     492     604    2025    2151    2739 
   Size:        492     112    1421     126     588

  Fragments arranged by size: 

                1421     588     492     126     112
 
PshAI GACnn'nnGTC

Cuts at:      0    1659    2739 
   Size:       1659    1080
 
PstI C_TGCA'G

Cuts at:      0     461    2739 
   Size:        461    2278
 
PvuII CAG'CTG

Cuts at:      0    1600    1963    2338    2739 
   Size:       1600     363     375     401

  Fragments arranged by size: 

                1600     401     375     363
 
SacI G_AGCT'C

Cuts at:      0    2690    2739 
   Size:       2690      49
 
SexAI A'CCwGG_T

Cuts at:      0    2573    2739 
   Size:       2573     166
 
SfcI C'TryA_G

Cuts at:      0     457     931    1093    2739 
   Size:        457     474     162    1646

  Fragments arranged by size: 

                1646     474     457     162
 
SmlI C'TyrA_G

Cuts at:      0     906    2739 
   Size:        906    1833
 
SphI G_CATG'C

Cuts at:      0    1927    2223    2739 
   Size:       1927     296     516

  Fragments arranged by size: 

                1927     516     296
 
StyI C'CwwG_G

Cuts at:      0    1129    2043    2435    2718    2739 
   Size:       1129     914     392     283      21

  Fragments arranged by size: 

                1129     914     392     283      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1624    2017    2089    2143    2645    2739 
   Size:       1624     393      72      54     502      94

  Fragments arranged by size: 

                1624     502     393      94      72      54
 
TatI w'GTAC_w

Cuts at:      0     768    1860    2603    2739 
   Size:        768    1092     743     136

  Fragments arranged by size: 

                1092     768     743     136
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     699     731     944     976    1830    1862    2533    2565 
   Size:        699      32     213      32     854      32     671      32

Cuts at:   2565    2739 
   Size:        174

  Fragments arranged by size: 

                 854     699     671     213     174      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0     148    2739 
   Size:        148    2591
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     493    2152    2293    2739 
   Size:        493    1659     141     446

  Fragments arranged by size: 

                1659     493     446     141
 
XmnI GAAnn'nnTTC

Cuts at:      0    1315    1848    2739 
   Size:       1315     533     891

  Fragments arranged by size: 

                1315     891     533
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI    ApaLI     ApoI    BamHI
     BanI    BanII     BbsI    BbvCI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     DraI     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII
  HindIII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NsiI     NspI    PflMI    PshAI     PstI    PvuII     SacI    SexAI
     SfcI     SmlI     SphI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BglII     BlpI     BmtI
     BplI    BsaAI    BsiEI    BsiWI    BspEI    BspHI   BssHII    BssSI
   BstAPI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI
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