Restriction map for pF1KA0464
 (Circular) (Six-base) MAPSORT of: pF1KA0464.seq  Check: 753  from: 1  to: 4085



pF1KA0464  4085 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AccI GT'mk_AC

Cuts at:   1123    1123 
   Size:       4085
 
Acc65I G'GTAC_C

Cuts at:   1101    1101 
   Size:       4085
 
AclI AA'CG_TT

Cuts at:   3925    3925 
   Size:       4085
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    382     462     498    1910    2342    3089    3357     382 
   Size:         80      36    1412     432     747     268    1110

  Fragments arranged by size: 

                1412    1110     747     432     268      80      36
 
AflIII A'CryG_T

Cuts at:   1437    2541    3518    1437 
   Size:       1104     977    2004

  Fragments arranged by size: 

                2004    1104     977
 
AgeI A'CCGG_T

Cuts at:   2473    2473 
   Size:       4085
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    644     644 
   Size:       4085
 
AlwNI CAG_nnn'CTG

Cuts at:   1390    2957    1390 
   Size:       1567    2518
 
ApaLI G'TGCA_C

Cuts at:   2855    2855 
   Size:       4085
 
ApoI r'AATT_y

Cuts at:    787    1089    3438    3694     787 
   Size:        302    2349     256    1178

  Fragments arranged by size: 

                2349    1178     302     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4085
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4085
 
AvaI C'yCGr_G

Cuts at:    491    1105     491 
   Size:        614    3471
 
BamHI G'GATC_C

Cuts at:    158    1110     158 
   Size:        952    3133
 
BanI G'GyrC_C

Cuts at:    499    1101    1515    1763    1798     499 
   Size:        602     414     248      35    2786

  Fragments arranged by size: 

                2786     602     414     248      35
 
BanII G_rGCy'C

Cuts at:    875    1099    2129     875 
   Size:        224    1030    2831

  Fragments arranged by size: 

                2831    1030     224
 
BbeI G_GCGC'C

Cuts at:   1519    1767    1519 
   Size:        248    3837
 
BbvCI CC'TCA_GC

Cuts at:   1039    1039 
   Size:       4085
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4051
 
BciVI GTATCCnnnnn_n'

Cuts at:    272    1983    2744    4085     272 
   Size:       1711     761    1341     272

  Fragments arranged by size: 

                1711    1341     761     272
 
BclI T'GATC_A

Cuts at:   1605    1605 
   Size:       4085
 
BglI GCCn_nnn'nGGC

Cuts at:    712     721     712 
   Size:          9    4076
 
BglII A'GATC_T

Cuts at:    473    1600    3637     473 
   Size:       1127    2037     921

  Fragments arranged by size: 

                2037    1127     921
 
BlpI GC'TnA_GC

Cuts at:    604    1197     604 
   Size:        593    3492
 
Bme1580I G_kGCm'C

Cuts at:    149     940    1710    1803    2859     149 
   Size:        791     770      93    1056    1375

  Fragments arranged by size: 

                1375    1056     791     770      93
 
BmgBI CAC'GTC

Cuts at:    231     392     231 
   Size:        161    3924
 
BmrI ACTGGGnnnn_n'

Cuts at:   1297    1378    1713    3857    1297 
   Size:         81     335    2144    1525

  Fragments arranged by size: 

                2144    1525     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    732    2452     732 
   Size:       1720    2365
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    722     911     964     976     722 
   Size:        189      53      12    3831

  Fragments arranged by size: 

                3831     189      53      12
 
Bpu10I CC'TnA_GC

Cuts at:    675    1039     675 
   Size:        364    3721
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1261    2140    2632    2930    1261 
   Size:        879     492     298    2416

  Fragments arranged by size: 

                2416     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    674    3789     674 
   Size:       3115     970
 
BsaAI yAC'GTr

Cuts at:    216    1438     216 
   Size:       1222    2863
 
BsaBI GATnn'nnATC

Cuts at:      6    1599    3636       6 
   Size:       1593    2037     455

  Fragments arranged by size: 

                2037    1593     455
 
BsaHI Gr'CG_yC

Cuts at:    179    1516    1764    2466    3962     179 
   Size:       1337     248     702    1496     302

  Fragments arranged by size: 

                1496    1337     702     302     248
 
BsaWI w'CCGG_w

Cuts at:   1274    1795    2473    2747    2894    3305    1274 
   Size:        521     678     274     147     411    2054

  Fragments arranged by size: 

                2054     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    338     368     832     862     338 
   Size:         30     464      30    3561

  Fragments arranged by size: 

                3561     464      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    748     967     979    1027    1036     748 
   Size:        219      12      48       9    3797

  Fragments arranged by size: 

                3797     219      48      12       9
 
BseYI C'CCAG_C

Cuts at:   1510    2268    2845    3341    1510 
   Size:        758     577     496    2254

  Fragments arranged by size: 

                2254     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1627    1673    2881     104 
   Size:       1523      46    1208    1308

  Fragments arranged by size: 

                1523    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1099    1877    2067    2859    1099 
   Size:        778     190     792    2325

  Fragments arranged by size: 

                2325     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    633     633 
   Size:       4085
 
Bsp1286I G_dGCh'C

Cuts at:    149     875     940    1099    1710    1803    1877    2067    2129 
   Size:        726      65     159     611      93      74     190      62

Cuts at:   2129    2859     149 
   Size:        730    1375

  Fragments arranged by size: 

                1375     730     726     611     190     159      93      74
                  65      62
 
BspEI T'CCGG_A

Cuts at:   3305    3305 
   Size:       4085
 
BspHI T'CATG_A

Cuts at:    453    3261     453 
   Size:       2808    1277
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    161    1135    1349    1651    2032     161 
   Size:        974     214     302     381    2214

  Fragments arranged by size: 

                2214     974     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2377    2431    3915    4080      50 
   Size:       2327      54    1484     165      55

  Fragments arranged by size: 

                2327    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1997    1997 
   Size:       4085
 
BsrFI r'CCGG_y

Cuts at:   2083    2264    2473    2083 
   Size:        181     209    3695

  Fragments arranged by size: 

                3695     209     181
 
BssSI C'ACGA_G

Cuts at:   2356    2714    2356 
   Size:        358    3727
 
BstAPI GCAn_nnn'nTGC

Cuts at:    153    1462     153 
   Size:       1309    2776
 
BstBI TT'CG_AA

Cuts at:   2446    2446 
   Size:       4085
 
BstXI CCAn_nnnn'nTGG

Cuts at:    646     700    1073     646 
   Size:         54     373    3658

  Fragments arranged by size: 

                3658     373      54
 
BstYI r'GATC_y

Cuts at:      1     158     437     473    1110    1577    1600    1935    2181 
   Size:        157     279      36     637     467      23     335     246

Cuts at:   2181    3193    3279    3291    3637       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     637     467     449     346     335     279     246
                 157      86      36      23      12
 
BtgI C'CryG_G

Cuts at:    719     719 
   Size:       4085
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2035    2216    2379    2035 
   Size:        181     163    3741

  Fragments arranged by size: 

                3741     181     163
 
BtsI GCAGTG_nn'

Cuts at:    725    1328     725 
   Size:        603    3482
 
ClaI AT'CG_AT

Cuts at:    746     746 
   Size:       4085
 
DraI TTT'AAA

Cuts at:    294     531    1084     294 
   Size:        237     553    3295

  Fragments arranged by size: 

                3295     553     237
 
DrdI GACnn_nn'nnGTC

Cuts at:    951    1791    2649     951 
   Size:        840     858    2387

  Fragments arranged by size: 

                2387     858     840
 
EaeI y'GGCC_r

Cuts at:    636     722    1670    1844    2235    2262    3607     636 
   Size:         86     948     174     391      27    1345    1114

  Fragments arranged by size: 

                1345    1114     948     391     174      86      27
 
EagI C'GGCC_G

Cuts at:   1670    1670 
   Size:       4085
 
EarI CTCTTCn'nnn_

Cuts at:    538    2108    2318     538 
   Size:       1570     210    2305

  Fragments arranged by size: 

                2305    1570     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2293    2603    2749    3895    2293 
   Size:        310     146    1146    2483

  Fragments arranged by size: 

                2483    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1097    1097 
   Size:       4085
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    382     462     498     722     911     964     976    1910    2342 
   Size:         80      36     224     189      53      12     934     432

Cuts at:   2342    3089    3357     382 
   Size:        747     268    1110

  Fragments arranged by size: 

                1110     934     747     432     268     224     189      80
                  53      36      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:    891     891 
   Size:       4085
 
EcoO109I rG'GnC_Cy

Cuts at:    423     713     759    1224     423 
   Size:        290      46     465    3284

  Fragments arranged by size: 

                3284     465     290      46
 
EcoRI G'AATT_C

Cuts at:    787    1089     787 
   Size:        302    3783
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2104    2104 
   Size:       4085
 
FspI TGC'GCA

Cuts at:   1866    1866 
   Size:       4085
 
HaeII r_GCGC'y

Cuts at:   1519    1767    2789    3768    1519 
   Size:        248    1022     979    1836

  Fragments arranged by size: 

                1836    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1927    1957    1959    1989    1927 
   Size:         30       2      30    4023

  Fragments arranged by size: 

                4023      30      30       2
 
HincII GTy'rAC

Cuts at:   1124    1124 
   Size:       4085
 
KasI G'GCGC_C

Cuts at:   1515    1763    1515 
   Size:        248    3837
 
KpnI G_GTAC'C

Cuts at:   1105    1105 
   Size:       4085
 
MluI A'CGCG_T

Cuts at:   3518    3518 
   Size:       4085
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2756    2940    2756 
   Size:        184    3901
 
MscI TGG'CCA

Cuts at:    638    1846     638 
   Size:       1208    2877
 
MslI CAynn'nnrTG

Cuts at:   1071    2074    2201    1071 
   Size:       1003     127    2955

  Fragments arranged by size: 

                2955    1003     127
 
MspA1I CmG'CkG

Cuts at:    951    1196    1510    1870    2883    3128     951 
   Size:        245     314     360    1013     245    1908

  Fragments arranged by size: 

                1908    1013     360     314     245     245
 
NaeI GCC'GGC

Cuts at:   2266    2266 
   Size:       4085
 
NarI GG'CG_CC

Cuts at:   1516    1764    1516 
   Size:        248    3837
 
NgoMIV G'CCGG_C

Cuts at:   2264    2264 
   Size:       4085
 
NspI r_CATG'y

Cuts at:   1138    1638    2545    1138 
   Size:        500     907    2678

  Fragments arranged by size: 

                2678     907     500
 
PciI A'CATG_T

Cuts at:   2541    2541 
   Size:       4085
 
PfoI T'CCnGG_A

Cuts at:    782     782 
   Size:       4085
 
PmeI GTTT'AAAC

Cuts at:   1084    1084 
   Size:       4085
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3224    3256    3224 
   Size:         32    4053
 
PpuMI rG'GwC_Cy

Cuts at:    423     759     423 
   Size:        336    3749
 
PstI C_TGCA'G

Cuts at:    614     732    1132    1817     614 
   Size:        118     400     685    2882

  Fragments arranged by size: 

                2882     685     400     118
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4085
 
PvuII CAG'CTG

Cuts at:   1510    1870    1510 
   Size:        360    3725
 
RsrII CG'GwC_CG

Cuts at:   2280    2280 
   Size:       4085
 
SacI G_AGCT'C

Cuts at:   1099    1099 
   Size:       4085
 
SalI G'TCGA_C

Cuts at:   1122    1122 
   Size:       4085
 
SapI GCTCTTCn'nnn_

Cuts at:    538    2108    2318     538 
   Size:       1570     210    2305

  Fragments arranged by size: 

                2305    1570     210
 
SbfI CC_TGCA'GG

Cuts at:   1132    1132 
   Size:       4085
 
ScaI AGT'ACT

Cuts at:   3602    3602 
   Size:       4085
 
SfcI C'TryA_G

Cuts at:     33     432     610     728     818    1128    1813    2806    2997 
   Size:        399     178     118      90     310     685     993     191

Cuts at:   2997      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     399     310     191     178     118
                  90
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    721     721 
   Size:       4085
 
SfoI GGC'GCC

Cuts at:   1517    1765    1517 
   Size:        248    3837
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4085
 
SmaI CCC'GGG

Cuts at:   1107    1107 
   Size:       4085
 
SmlI C'TyrA_G

Cuts at:    491    1240    2155    2647    2909     491 
   Size:        749     915     492     262    1667

  Fragments arranged by size: 

                1667     915     749     492     262
 
SnaBI TAC'GTA

Cuts at:    216     216 
   Size:       4085
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4085
 
SphI G_CATG'C

Cuts at:   1138    1638    1138 
   Size:        500    3585
 
StuI AGG'CCT

Cuts at:    583     932     583 
   Size:        349    3736
 
StyI C'CwwG_G

Cuts at:    235     851    1219    1573     235 
   Size:        616     368     354    2747

  Fragments arranged by size: 

                2747     616     368     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    906    1668    1801    2260    2469     906 
   Size:        762     133     459     209    2522

  Fragments arranged by size: 

                2522     762     459     209     133
 
TatI w'GTAC_w

Cuts at:    125    3600     125 
   Size:       3475     610
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    338     370     376     408     832     864    3602    3634     338 
   Size:         32       6      32     424      32    2738      32     789

  Fragments arranged by size: 

                2738     789     424      32      32      32      32       6
            
 
Tth111I GACn'n_nGTC

Cuts at:   1882    1882 
   Size:       4085
 
XbaI T'CTAG_A

Cuts at:    810    1116     810 
   Size:        306    3779
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    249    1580     249 
   Size:       1331    2754
 
XhoI C'TCGA_G

Cuts at:    491     491 
   Size:       4085
 
XmaI C'CCGG_G

Cuts at:   1105    1105 
   Size:       4085
 
XmnI GAAnn'nnTTC

Cuts at:    475     475 
   Size:       4085
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmeI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BfrBI     BmtI     BsgI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I    CspCI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
    SgrAI     SrfI     SspI     SwaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com