Restriction map for pF1KA0428
 (Linear) (Six-base) MAPSORT of: KIAA0428.seq  Check: 1026  from: 1  to: 1110



KIAA0428.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     505     514     655     991    1110 
   Size:        505       9     141     336     119

  Fragments arranged by size: 

                 505     336     141     119       9
 
ApaLI G'TGCA_C

Cuts at:      0     970    1110 
   Size:        970     140
 
ApoI r'AATT_y

Cuts at:      0     103     809    1110 
   Size:        103     706     301

  Fragments arranged by size: 

                 706     301     103
 
AseI AT'TA_AT

Cuts at:      0     526    1110 
   Size:        526     584
 
AvaI C'yCGr_G

Cuts at:      0     689    1110 
   Size:        689     421
 
BanI G'GyrC_C

Cuts at:      0     325     868    1110 
   Size:        325     543     242

  Fragments arranged by size: 

                 543     325     242
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      98     132    1110 
   Size:         98      34     978

  Fragments arranged by size: 

                 978      98      34
 
BfrBI ATG'CAT

Cuts at:      0     382    1110 
   Size:        382     728
 
BglI GCCn_nnn'nGGC

Cuts at:      0     286    1110 
   Size:        286     824
 
BglII A'GATC_T

Cuts at:      0     443    1110 
   Size:        443     667
 
BlpI GC'TnA_GC

Cuts at:      0     369    1110 
   Size:        369     741
 
Bme1580I G_kGCm'C

Cuts at:      0     330     974    1110 
   Size:        330     644     136

  Fragments arranged by size: 

                 644     330     136
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     704     866    1110 
   Size:        704     162     244

  Fragments arranged by size: 

                 704     244     162
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168    1110 
   Size:        168     942
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     800    1110 
   Size:        565     235     310

  Fragments arranged by size: 

                 565     310     235
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     599    1110 
   Size:        599     511
 
BseYI C'CCAG_C

Cuts at:      0     290    1110 
   Size:        290     820
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     440     500     704     761    1110 
   Size:        440      60     204      57     349

  Fragments arranged by size: 

                 440     349     204      60      57
 
BsiHKAI G_wGCw'C

Cuts at:      0     974    1110 
   Size:        974     136
 
Bsp1286I G_dGCh'C

Cuts at:      0     330     974    1110 
   Size:        330     644     136

  Fragments arranged by size: 

                 644     330     136
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     504    1110 
   Size:        504     606
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     505     514     784    1110 
   Size:        505       9     270     326

  Fragments arranged by size: 

                 505     326     270       9
 
BstBI TT'CG_AA

Cuts at:      0     118    1110 
   Size:        118     992
 
BstYI r'GATC_y

Cuts at:      0     443    1110 
   Size:        443     667
 
BtgI C'CryG_G

Cuts at:      0     803     965    1110 
   Size:        803     162     145

  Fragments arranged by size: 

                 803     162     145
 
DraI TTT'AAA

Cuts at:      0     224    1110 
   Size:        224     886
 
DraIII CAC_nnn'GTG

Cuts at:      0     659    1110 
   Size:        659     451
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     649    1110 
   Size:        649     461
 
EaeI y'GGCC_r

Cuts at:      0      85     278    1110 
   Size:         85     193     832

  Fragments arranged by size: 

                 832     193      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168    1110 
   Size:        168     942
 
EcoO109I rG'GnC_Cy

Cuts at:      0     412    1110 
   Size:        412     698
 
EcoRI G'AATT_C

Cuts at:      0     809    1110 
   Size:        809     301
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     841    1110 
   Size:        841     269
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     134     166    1110 
   Size:        134      32     944

  Fragments arranged by size: 

                 944     134      32
 
HincII GTy'rAC

Cuts at:      0     762    1110 
   Size:        762     348
 
HindIII A'AGCT_T

Cuts at:      0     366    1110 
   Size:        366     744
 
MfeI C'AATT_G

Cuts at:      0     573    1110 
   Size:        573     537
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215    1110 
   Size:        215     895
 
MscI TGG'CCA

Cuts at:      0     280    1110 
   Size:        280     830
 
MslI CAynn'nnrTG

Cuts at:      0     280    1094    1110 
   Size:        280     814      16

  Fragments arranged by size: 

                 814     280      16
 
MspA1I CmG'CkG

Cuts at:      0     502     511     775     799    1110 
   Size:        502       9     264      24     311

  Fragments arranged by size: 

                 502     311     264      24       9
 
NcoI C'CATG_G

Cuts at:      0     803     965    1110 
   Size:        803     162     145

  Fragments arranged by size: 

                 803     162     145
 
NsiI A_TGCA'T

Cuts at:      0     384    1110 
   Size:        384     726
 
NspI r_CATG'y

Cuts at:      0     927    1110 
   Size:        927     183
 
PpuMI rG'GwC_Cy

Cuts at:      0     412    1110 
   Size:        412     698
 
PstI C_TGCA'G

Cuts at:      0     501     510     774     789    1110 
   Size:        501       9     264      15     321

  Fragments arranged by size: 

                 501     321     264      15       9
 
PvuII CAG'CTG

Cuts at:      0     502     511     775     799    1110 
   Size:        502       9     264      24     311

  Fragments arranged by size: 

                 502     311     264      24       9
 
SexAI A'CCwGG_T

Cuts at:      0     755    1110 
   Size:        755     355
 
SfcI C'TryA_G

Cuts at:      0     497     506     770     785    1110 
   Size:        497       9     264      15     325

  Fragments arranged by size: 

                 497     325     264      15       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     286    1110 
   Size:        286     824
 
SmlI C'TyrA_G

Cuts at:      0     544     815    1110 
   Size:        544     271     295

  Fragments arranged by size: 

                 544     295     271
 
StuI AGG'CCT

Cuts at:      0     846    1110 
   Size:        846     264
 
StyI C'CwwG_G

Cuts at:      0     803     965    1110 
   Size:        803     162     145

  Fragments arranged by size: 

                 803     162     145
 
TatI w'GTAC_w

Cuts at:      0     822    1110 
   Size:        822     288
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     468     500    1008    1040    1110 
   Size:        468      32     508      32      70

  Fragments arranged by size: 

                 508     468      70      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     136    1110 
   Size:        136     974
 

 Enzymes that do cut:

    AlwNI    ApaLI     ApoI     AseI     AvaI     BanI     BcgI    BfrBI
     BglI    BglII     BlpI Bme1580I     BplI     BpmI    BpuEI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI    BstBI    BstYI
     BtgI     DraI   DraIII     DrdI     EaeI  Eco57MI EcoO109I    EcoRI
     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NsiI     NspI    PpuMI     PstI    PvuII    SexAI
     SfcI     SfiI     SmlI     StuI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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