Restriction map for pF1KA0411
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AscI        
AseI        AsnI        Asp718I     AviII       BbrPI       
BciVI       BclI        BfrI        BglII       BsaAI       
BsaBI       BsiWI       BsmBI       BsrBI       BssHII      
Bst1107I    BstBI       BstZ17I     CpoI        DraIII      
EcoNI       EcoRI       FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NcoI        NdeI        
NheI        NotI        NspV        PacI        PflMI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    RsrII       SacII       SapI        SbfI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SwaI        Van91I      XbaI        
XcmI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         2742  GTCGAC               GT^[AC][GT]AC
AccIII        842  TCCGGA               T^CCGGA
AgeI         3125  ACCGGT               A^CCGGT
Alw44I       1765  GTGCAC               G^TGCAC
AocI         1705  CCTAAGG              CC^T[ACGT]AGG
ApaI         2565  GGGCCC               GGGCC^C
ApaLI        1765  GTGCAC               G^TGCAC
AspI         1221  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2772  CCTAGG               C^CTAGG
BamHI        1878  GGATCC               G^GATCC
BbvCI         591  CCTCAGC              CCTCAGC(-5/-2)
BlnI         2772  CCTAGG               C^CTAGG
BseAI         842  TCCGGA               T^CCGGA
BsgI         2842  CTGCAC               GTGCAG(16/14)
Bsp1407I     2325  TGTACA               T^GTACA
BspEI         842  TCCGGA               T^CCGGA
BsrGI        2325  TGTACA               T^GTACA
BstAPI       2844  GCACGAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        465  GGTGACC              G^GT[ACGT]ACC
BstPI         465  GGTGACC              G^GT[ACGT]ACC
BstXI        2118  CCATGACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1705  CCTAAGG              CC^T[ACGT]AGG
DrdI         1183  GACTTTGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2441  CCACGG               C^C[GA][CT]GG
Eco81I       1705  CCTAAGG              CC^T[ACGT]AGG
EcoO65I       465  GGTGACC              G^GT[ACGT]ACC
EcoT14I      2772  CCTAGG               C^C[AT][AT]GG
EcoT22I      2049  ATGCAT               ATGCA^T
MaeII        2674  ACGT                 A^CGT
MroI          842  TCCGGA               T^CCGGA
NaeI         2279  GCCGGC               GCC^GGC
NgoMIV       2279  GCCGGC               G^CCGGC
NruI          121  TCGCGA               TCG^CGA
NsiI         2049  ATGCAT               ATGCA^T
PflFI        1221  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3125  ACCGGT               A^CCGGT
PvuI         1216  CGATCG               CGAT^CG
SalI         2742  GTCGAC               G^TCGAC
SfiI         3101  GGCCCGTGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        2325  TGTACA               T^GTACA
SspI         2069  AATATT               AAT^ATT
StuI         2411  AGGCCT               AGG^CCT
StyI         2772  CCTAGG               C^C[AT][AT]GG
Tth111I      1221  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI         1324  AAATTT               [GA]^AATT[CT]
             1590  AAATTC              
AflIII        503  ACATGT               A^C[GA][CT]GT
              959  ACATGT              
AlwNI        1393  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1652  CAGTCGCTG           
ApoI         1324  AAATTT               [GA]^AATT[CT]
             1590  AAATTC              
BalI         1097  TGGCCA               TGG^CCA
             1892  TGGCCA              
BbeI         2886  GGCGCC               GGCGC^C
             3073  GGCGCC              
BcgI         2169  CGAGGCTATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2985  CGACCCCGATGC        
BfaI         2026  CTAG                 C^TAG
             2773  CTAG                
BsaWI         842  TCCGGA               [AT]^CCGG[AT]
             3125  ACCGGT              
BsmI          909  GAATGC               GAATGC(1/-1)
             1420  GAATGC              
BspDI         796  ATCGAT               AT^CGAT
             2104  ATCGAT              
BspLU11I      503  ACATGT               A^CATGT
              959  ACATGT              
BspMI         602  ACCTGC               ACCTGC(4/8)
             2746  ACCTGC              
ClaI          796  ATCGAT               AT^CGAT
             2104  ATCGAT              
DraI         1327  TTTAAA               TTT^AAA
             1665  TTTAAA              
EagI         3109  CGGCCG               C^GGCCG
             3164  CGGCCG              
EclXI        3109  CGGCCG               C^GGCCG
             3164  CGGCCG              
Eco52I       3109  CGGCCG               C^GGCCG
             3164  CGGCCG              
EcoRV        1642  GATATC               GAT^ATC
             1728  GATATC              
HgaI         2522  GACGC                GACGC(5/10)
             3171  GACGC               
HincII       2113  GTTGAC               GT[CT]^[GA]AC
             2742  GTCGAC              
HindII       2113  GTTGAC               GT[CT]^[GA]AC
             2742  GTCGAC              
KasI         2886  GGCGCC               G^GCGCC
             3073  GGCGCC              
MaeI         2026  CTAG                 C^TAG
             2773  CTAG                
MluNI        1097  TGGCCA               TGG^CCA
             1892  TGGCCA              
MscI         1097  TGGCCA               TGG^CCA
             1892  TGGCCA              
NarI         2886  GGCGCC               GG^CGCC
             3073  GGCGCC              
PaeR7I        181  CTCGAG               C^TCGAG
             2860  CTCGAG              
PpuMI        1937  AGGACCC              [GA]G^G[AT]CC[CT]
             2031  GGGACCT             
ScaI          185  AGTACT               AGT^ACT
              683  AGTACT              
SfoI         2886  GGCGCC               GGC^GCC
             3073  GGCGCC              
TfiI          928  GATTC                G^A[AT]TC
             1468  GATTC               
XhoI          181  CTCGAG               C^TCGAG
             2860  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        Aor51HI     Asp700I     AspEI       
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BsrDI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
Eam1105I    EarI        Eco47III    EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RcaI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmaCI       XmaI        XmnI        
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