Restriction map for pF1KA0411
 (Linear) (Six-base) MAPSORT of: KIAA0411.seq  Check: 8783  from: 1  to: 3225



KIAA0411.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AccI GT'mk_AC

Cuts at:      0    2743    3225 
   Size:       2743     482
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     562    1533    2941    3225 
   Size:        481      81     971    1408     284

  Fragments arranged by size: 

                1408     971     481     284      81
 
AfeI AGC'GCT

Cuts at:      0     217    1038    2685    3225 
   Size:        217     821    1647     540

  Fragments arranged by size: 

                1647     821     540     217
 
AflIII A'CryG_T

Cuts at:      0     503     959    3225 
   Size:        503     456    2266

  Fragments arranged by size: 

                2266     503     456
 
AgeI A'CCGG_T

Cuts at:      0    3125    3225 
   Size:       3125     100
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1944    2433    2784    3225 
   Size:       1944     489     351     441

  Fragments arranged by size: 

                1944     489     441     351
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     619    2857    3225 
   Size:        619    2238     368

  Fragments arranged by size: 

                2238     619     368
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     238     270     556     588    3225 
   Size:        238      32     286      32    2637

  Fragments arranged by size: 

                2637     286     238      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1398    1657    3225 
   Size:       1398     259    1568

  Fragments arranged by size: 

                1568    1398     259
 
ApaI G_GGCC'C

Cuts at:      0    2569    3225 
   Size:       2569     656
 
ApaLI G'TGCA_C

Cuts at:      0    1765    3225 
   Size:       1765    1460
 
ApoI r'AATT_y

Cuts at:      0    1324    1590    3225 
   Size:       1324     266    1635

  Fragments arranged by size: 

                1635    1324     266
 
AvaI C'yCGr_G

Cuts at:      0     181     242    2018    2555    2588    2633    2860    3147 
   Size:        181      61    1776     537      33      45     227     287

Cuts at:   3147    3225 
   Size:         78

  Fragments arranged by size: 

                1776     537     287     227     181      78      61      45
                  33
 
AvrII C'CTAG_G

Cuts at:      0    2772    3225 
   Size:       2772     453
 
BamHI G'GATC_C

Cuts at:      0    1878    3225 
   Size:       1878    1347
 
BanI G'GyrC_C

Cuts at:      0    1426    2886    3073    3217    3225 
   Size:       1426    1460     187     144       8

  Fragments arranged by size: 

                1460    1426     187     144       8
 
BanII G_rGCy'C

Cuts at:      0     182     458     479    2569    2653    2918    2954    3225 
   Size:        182     276      21    2090      84     265      36     271

  Fragments arranged by size: 

                2090     276     271     265     182      84      36      21
            
 
BbeI G_GCGC'C

Cuts at:      0    2890    3077    3225 
   Size:       2890     187     148

  Fragments arranged by size: 

                2890     187     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     966    1616    2819    2830    3225 
   Size:        966     650    1203      11     395

  Fragments arranged by size: 

                1203     966     650     395      11
 
BbvCI CC'TCA_GC

Cuts at:      0     592    3225 
   Size:        592    2633
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2158    2192    2974    3008    3225 
   Size:       2158      34     782      34     217

  Fragments arranged by size: 

                2158     782     217      34      34
 
BfrBI ATG'CAT

Cuts at:      0    2051    3225 
   Size:       2051    1174
 
BglI GCCn_nnn'nGGC

Cuts at:      0     829    3067    3071    3108    3225 
   Size:        829    2238       4      37     117

  Fragments arranged by size: 

                2238     829     117      37       4
 
BlpI GC'TnA_GC

Cuts at:      0     212    1040    3201    3225 
   Size:        212     828    2161      24

  Fragments arranged by size: 

                2161     828     212      24
 
Bme1580I G_kGCm'C

Cuts at:      0    1769    2569    2732    3220    3225 
   Size:       1769     800     163     488       5

  Fragments arranged by size: 

                1769     800     488     163       5
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1848    3225 
   Size:       1848    1377
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     237    2469    2855    3225 
   Size:        237    2232     386     370

  Fragments arranged by size: 

                2232     386     370     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     130     220     892    1516    1765    2078    2416 
   Size:        124       6      90     672     624     249     313     338

Cuts at:   2416    2812    2932    3225 
   Size:        396     120     293

  Fragments arranged by size: 

                 672     624     396     338     313     293     249     124
                 120      90       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     592    2359    3225 
   Size:        592    1767     866

  Fragments arranged by size: 

                1767     866     592
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     973    1090    1372    3225 
   Size:        973     117     282    1853

  Fragments arranged by size: 

                1853     973     282     117
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     326     581     868    1243    3225 
   Size:        326     255     287     375    1982

  Fragments arranged by size: 

                1982     375     326     287     255
 
BsaHI Gr'CG_yC

Cuts at:      0    2523    2887    3074    3172    3225 
   Size:       2523     364     187      98      53

  Fragments arranged by size: 

                2523     364     187      98      53
 
BsaWI w'CCGG_w

Cuts at:      0     842    3125    3225 
   Size:        842    2283     100

  Fragments arranged by size: 

                2283     842     100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     238     268     556     586    1834    1864    2254    2284 
   Size:        238      30     288      30    1248      30     390      30

Cuts at:   2284    3225 
   Size:        941

  Fragments arranged by size: 

                1248     941     390     288     238      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     213     265     466     577    1135    1296    3009    3225 
   Size:        213      52     201     111     558     161    1713     216

  Fragments arranged by size: 

                1713     558     216     213     201     161     111      52
            
 
BseYI C'CCAG_C

Cuts at:      0     626    1752    2011    2569    2605    3077    3225 
   Size:        626    1126     259     558      36     472     148

  Fragments arranged by size: 

                1126     626     558     472     259     148      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2827    3225 
   Size:       2827     398
 
BsiEI CG_ry'CG

Cuts at:      0    1219    3112    3167    3225 
   Size:       1219    1893      55      58

  Fragments arranged by size: 

                1893    1219      58      55
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     251     458     479    1769    2450    2836    3225 
   Size:        182      69     207      21    1290     681     386     389

  Fragments arranged by size: 

                1290     681     389     386     207     182      69      21
            
 
BsmI GAATG_Cn'

Cuts at:      0     915    1426    3225 
   Size:        915     511    1799

  Fragments arranged by size: 

                1799     915     511
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     251     458     479    1769    2450    2569    2653 
   Size:        182      69     207      21    1290     681     119      84

Cuts at:   2653    2732    2836    2918    2954    3220    3225 
   Size:         79     104      82      36     266       5

  Fragments arranged by size: 

                1290     681     266     207     182     119     104      84
                  82      79      69      36      21       5
 
BspEI T'CCGG_A

Cuts at:      0     842    3225 
   Size:        842    2383
 
BspHI T'CATG_A

Cuts at:      0     359     488    1817    1995    3225 
   Size:        359     129    1329     178    1230

  Fragments arranged by size: 

                1329    1230     359     178     129
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     611    2755    3225 
   Size:        611    2144     470

  Fragments arranged by size: 

                2144     611     470
 
BsrDI GCAATG_nn'

Cuts at:      0      38    1271    2395    3225 
   Size:         38    1233    1124     830

  Fragments arranged by size: 

                1233    1124     830      38
 
BsrFI r'CCGG_y

Cuts at:      0     620    2279    3111    3125    3225 
   Size:        620    1659     832      14     100

  Fragments arranged by size: 

                1659     832     620     100      14
 
BsrGI T'GTAC_A

Cuts at:      0    2325    3225 
   Size:       2325     900
 
BssSI C'ACGA_G

Cuts at:      0     448     613    2845    3225 
   Size:        448     165    2232     380

  Fragments arranged by size: 

                2232     448     380     165
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2850    3225 
   Size:       2850     375
 
BstEII G'GTnAC_C

Cuts at:      0     465    3225 
   Size:        465    2760
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2125    3225 
   Size:       2125    1100
 
BstYI r'GATC_y

Cuts at:      0      68    1544    1561    1772    1878    3225 
   Size:         68    1476      17     211     106    1347

  Fragments arranged by size: 

                1476    1347     211     106      68      17
 
Bsu36I CC'TnA_GG

Cuts at:      0    1706    3225 
   Size:       1706    1519
 
BtgI C'CryG_G

Cuts at:      0    2441    3225 
   Size:       2441     784
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2162    3225 
   Size:       2162    1063
 
BtsI GCAGTG_nn'

Cuts at:      0     920    2394    3225 
   Size:        920    1474     831

  Fragments arranged by size: 

                1474     920     831
 
ClaI AT'CG_AT

Cuts at:      0     797    2105    3225 
   Size:        797    1308    1120

  Fragments arranged by size: 

                1308    1120     797
 
DraI TTT'AAA

Cuts at:      0    1329    1667    3225 
   Size:       1329     338    1558

  Fragments arranged by size: 

                1558    1329     338
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1189    3225 
   Size:       1189    2036
 
EaeI y'GGCC_r

Cuts at:      0    1097    1892    3109    3164    3225 
   Size:       1097     795    1217      55      61

  Fragments arranged by size: 

                1217    1097     795      61      55
 
EagI C'GGCC_G

Cuts at:      0    3109    3164    3225 
   Size:       3109      55      61

  Fragments arranged by size: 

                3109      61      55
 
EarI CTCTTCn'nnn_

Cuts at:      0     418     664    1834    2421    3225 
   Size:        418     246    1170     587     804

  Fragments arranged by size: 

                1170     804     587     418     246
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     148     367    3048    3073    3225 
   Size:        148     219    2681      25     152

  Fragments arranged by size: 

                2681     219     152     148      25
 
EcoICRI GAG'CTC

Cuts at:      0     180     456     477    3225 
   Size:        180     276      21    2748

  Fragments arranged by size: 

                2748     276     180      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     130     220     481     562     892    1516    1533 
   Size:        124       6      90     261      81     330     624      17

Cuts at:   1533    1765    2078    2416    2812    2932    2941    3225 
   Size:        232     313     338     396     120       9     284

  Fragments arranged by size: 

                 624     396     338     330     313     284     261     232
                 124     120      90      81      17       9       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1938    2032    2231    2559    2566    2802    2935    3225 
   Size:       1938      94     199     328       7     236     133     290

  Fragments arranged by size: 

                1938     328     290     236     199     133      94       7
            
 
EcoRV GAT'ATC

Cuts at:      0    1644    1730    3225 
   Size:       1644      86    1495

  Fragments arranged by size: 

                1644    1495      86
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1633    3225 
   Size:       1633    1592
 
HaeII r_GCGC'y

Cuts at:      0     219     633    1040    2687    2719    2890    3077    3225 
   Size:        219     414     407    1647      32     171     187     148

  Fragments arranged by size: 

                1647     414     407     219     187     171     148      32
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129    1714    1746    1930    1962    2332    2364 
   Size:         97      32    1585      32     184      32     370      32

Cuts at:   2364    2398    2419    2430    2451    2498    2530    2770    2802 
   Size:         34      21      11      21      47      32     240      32

Cuts at:   2802    3225 
   Size:        423

  Fragments arranged by size: 

                1585     423     370     240     184      97      47      34
                  32      32      32      32      32      32      21      21
                  11
 
HincII GTy'rAC

Cuts at:      0    2115    2744    3225 
   Size:       2115     629     481

  Fragments arranged by size: 

                2115     629     481
 
KasI G'GCGC_C

Cuts at:      0    2886    3073    3225 
   Size:       2886     187     152

  Fragments arranged by size: 

                2886     187     152
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1230    1800    2375    3225 
   Size:       1230     570     575     850

  Fragments arranged by size: 

                1230     850     575     570
 
MscI TGG'CCA

Cuts at:      0    1099    1894    3225 
   Size:       1099     795    1331

  Fragments arranged by size: 

                1331    1099     795
 
MslI CAynn'nnrTG

Cuts at:      0     358     487     952     958    1964    2123    3028    3225 
   Size:        358     129     465       6    1006     159     905     197

  Fragments arranged by size: 

                1006     905     465     358     197     159     129       6
            
 
MspA1I CmG'CkG

Cuts at:      0      81     132     859    1083    3205    3225 
   Size:         81      51     727     224    2122      20

  Fragments arranged by size: 

                2122     727     224      81      51      20
 
NaeI GCC'GGC

Cuts at:      0    2281    3225 
   Size:       2281     944
 
NarI GG'CG_CC

Cuts at:      0    2887    3074    3225 
   Size:       2887     187     151

  Fragments arranged by size: 

                2887     187     151
 
NgoMIV G'CCGG_C

Cuts at:      0    2279    3225 
   Size:       2279     946
 
NruI TCG'CGA

Cuts at:      0     123    3225 
   Size:        123    3102
 
NsiI A_TGCA'T

Cuts at:      0    2053    3225 
   Size:       2053    1172
 
NspI r_CATG'y

Cuts at:      0     507     963    1170    3225 
   Size:        507     456     207    2055

  Fragments arranged by size: 

                2055     507     456     207
 
PasI CC'CwG_GG

Cuts at:      0    1398    3225 
   Size:       1398    1827
 
PciI A'CATG_T

Cuts at:      0     503     959    3225 
   Size:        503     456    2266

  Fragments arranged by size: 

                2266     503     456
 
PfoI T'CCnGG_A

Cuts at:      0     146    3225 
   Size:        146    3079
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270     556     588    3172    3204    3225 
   Size:        238      32     286      32    2584      32      21

  Fragments arranged by size: 

                2584     286     238      32      32      32      21
 
PpuMI rG'GwC_Cy

Cuts at:      0    1938    2032    3225 
   Size:       1938      94    1193

  Fragments arranged by size: 

                1938    1193      94
 
PspOMI G'GGCC_C

Cuts at:      0    2565    3225 
   Size:       2565     660
 
PspXI vC'TCGA_Gb

Cuts at:      0     181    3225 
   Size:        181    3044
 
PstI C_TGCA'G

Cuts at:      0     131     443    1088    1370    3225 
   Size:        131     312     645     282    1855

  Fragments arranged by size: 

                1855     645     312     282     131
 
PvuI CG_AT'CG

Cuts at:      0    1219    3225 
   Size:       1219    2006
 
PvuII CAG'CTG

Cuts at:      0      81     132     859    1083    3225 
   Size:         81      51     727     224    2142

  Fragments arranged by size: 

                2142     727     224      81      51
 
SacI G_AGCT'C

Cuts at:      0     182     458     479    3225 
   Size:        182     276      21    2746

  Fragments arranged by size: 

                2746     276     182      21
 
SalI G'TCGA_C

Cuts at:      0    2742    3225 
   Size:       2742     483
 
ScaI AGT'ACT

Cuts at:      0     187     685    3225 
   Size:        187     498    2540

  Fragments arranged by size: 

                2540     498     187
 
SfcI C'TryA_G

Cuts at:      0     127     439    1084    1366    3225 
   Size:        127     312     645     282    1859

  Fragments arranged by size: 

                1859     645     312     282     127
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3108    3225 
   Size:       3108     117
 
SfoI GGC'GCC

Cuts at:      0    2888    3075    3225 
   Size:       2888     187     150

  Fragments arranged by size: 

                2888     187     150
 
SmaI CCC'GGG

Cuts at:      0     244    2020    2557    2590    2635    3225 
   Size:        244    1776     537      33      45     590

  Fragments arranged by size: 

                1776     590     537     244      45      33
 
SmlI C'TyrA_G

Cuts at:      0     181     988    1105    1387    2860    3225 
   Size:        181     807     117     282    1473     365

  Fragments arranged by size: 

                1473     807     365     282     181     117
 
SspI AAT'ATT

Cuts at:      0    2071    3225 
   Size:       2071    1154
 
StuI AGG'CCT

Cuts at:      0    2413    3225 
   Size:       2413     812
 
StyI C'CwwG_G

Cuts at:      0    2772    3225 
   Size:       2772     453
 
TatI w'GTAC_w

Cuts at:      0     185     683    2315    2325    3225 
   Size:        185     498    1632      10     900

  Fragments arranged by size: 

                1632     900     498     185      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1553    1585    2254    2286    3225 
   Size:       1553      32     669      32     939

  Fragments arranged by size: 

                1553     939     669      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1224    3225 
   Size:       1224    2001
 
XhoI C'TCGA_G

Cuts at:      0     181    2860    3225 
   Size:        181    2679     365

  Fragments arranged by size: 

                2679     365     181
 
XmaI C'CCGG_G

Cuts at:      0     242    2018    2555    2588    2633    3225 
   Size:        242    1776     537      33      45     592

  Fragments arranged by size: 

                1776     592     537     242      45      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     571    1715    3187    3225 
   Size:        571    1144    1472      38

  Fragments arranged by size: 

                1472    1144     571      38
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AgeI     AhdI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NruI
     NsiI     NspI     PasI     PciI     PfoI     PpiI    PpuMI   PspOMI
    PspXI     PstI     PvuI    PvuII     SacI     SalI     ScaI     SfcI
     SfiI     SfoI     SmaI     SmlI     SspI     StuI     StyI     TatI
     TstI  Tth111I     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AleI     AscI     AseI
    AsiSI     BaeI    BciVI     BclI    BglII    BmgBI     BmtI    BsaAI
    BsaBI    BsiWI    BsmBI    BsrBI   BssHII    BstBI  BstZ17I    CspCI
   DraIII    EcoNI    EcoRI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI     PacI
    PflMI     PmeI     PmlI    PshAI     PsiI     PsrI    RsrII    SacII
    SanDI     SapI     SbfI    SexAI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SwaI    TaqII     XbaI     XcmI     ZraI
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