Restriction map for
pF1KA0409
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII BamHI BbeI BbrPI BcgI
BclI BcnI BfrI BglII BsaAI
BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI HphI KasI KpnI
MaeII MamI MfeI MluI MslI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SexAI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 977 TCCGGA T^CCGGA
AcsI 426 AAATTC [GA]^AATT[CT]
ApoI 426 AAATTC [GA]^AATT[CT]
AvaI 698 CTCGGG C^[CT]CG[GA]G
AvrII 1018 CCTAGG C^CTAGG
BanI 618 GGTGCC G^G[CT][GA]CC
BciVI 974 GTATCC GTATCC(6/5)
BlnI 1018 CCTAGG C^CTAGG
BlpI 1321 GCTCAGC GC^T[ACGT]AGC
BpmI 1054 CTGGAG CTGGAG(16/14)
Bpu1102I 1321 GCTCAGC GC^T[ACGT]AGC
BsaI 1156 GGTCTC GGTCTC(1/5)
BsaWI 977 TCCGGA [AT]^CCGG[AT]
BseAI 977 TCCGGA T^CCGGA
BsgI 794 CTGCAC GTGCAG(16/14)
BsmBI 802 CGTCTC CGTCTC(1/5)
BsoBI 698 CTCGGG C^[CT]CG[GA]G
BspEI 977 TCCGGA T^CCGGA
BsrDI 991 CATTGC GCAATG(2/0)
BsrFI 569 ACCGGC [GA]^CCGG[CT]
BstAPI 235 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1287 CCAAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 903 GGATCT [GA]^GATC[CT]
CelII 1321 GCTCAGC GC^T[ACGT]AGC
Cfr10I 569 ACCGGC [GA]^CCGG[CT]
DraIII 1026 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
HaeII 107 AGCGCC [GA]GCGC^[CT]
HgaI 801 GCGTC GACGC(5/10)
HincII 451 GTTGAC GT[CT]^[GA]AC
HindII 451 GTTGAC GT[CT]^[GA]AC
MflI 903 GGATCT [GA]^GATC[CT]
MroI 977 TCCGGA T^CCGGA
MseI 547 TTAA T^TAA
NspI 414 ACATGC [GA]CATG^[CT]
PvuII 635 CAGCTG CAG^CTG
SapI 7 GAAGAGC GCTCTTC(1/4)
SfiI 21 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1332 AGGCCT AGG^CCT
Tru9I 547 TTAA T^TAA
Tsp509I 427 AATT ^AATT
XhoII 903 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 42 CGCG CG^CG
844 CGCG
AlwI 496 GATCC GGATC(4/5)
903 GGATC
AlwNI 436 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
730 CAGCGGCTG
AocI 613 CCTCAGG CC^T[ACGT]AGG
820 CCTGAGG
BalI 122 TGGCCA TGG^CCA
871 TGGCCA
BbsI 654 GAAGAC GAAGAC(2/6)
814 GAAGAC
BbvCI 637 GCTGAGG CCTCAGC(-5/-2)
1171 GCTGAGG
BpuAI 654 GAAGAC GAAGAC(2/6)
814 GAAGAC
BsmFI 1115 GGGAC GGGAC(10/14)
464 GTCCC
BstBI 4 TTCGAA TT^CGAA
1199 TTCGAA
BstUI 42 CGCG CG^CG
844 CGCG
Bsu36I 613 CCTCAGG CC^T[ACGT]AGG
820 CCTGAGG
CfoI 108 GCGC GCG^C
1362 GCGC
DpnII 496 GATC ^GATC
904 GATC
DrdI 657 GACAGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1015 GACCCTAGGGTC
DsaI 41 CCGCGG C^C[GA][CT]GG
843 CCGCGG
EaeI 122 TGGCCA [CT]^GGCC[GA]
871 TGGCCA
EarI 805 CTCTTC CTCTTC(1/4)
7 GAAGAG
Eco81I 613 CCTCAGG CC^T[ACGT]AGG
820 CCTGAGG
EcoNI 1122 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1303 CCTCTGGTAGG
HapII 570 CCGG C^CGG
978 CCGG
HhaI 108 GCGC GCG^C
1362 GCGC
HinP1I 108 GCGC G^CGC
1362 GCGC
HpaII 570 CCGG C^CGG
978 CCGG
Ksp632I 805 CTCTTC CTCTTC(1/4)
7 GAAGAG
KspI 41 CCGCGG CCGC^GG
843 CCGCGG
MboI 496 GATC ^GATC
904 GATC
MluNI 122 TGGCCA TGG^CCA
871 TGGCCA
MscI 122 TGGCCA TGG^CCA
871 TGGCCA
MspI 570 CCGG C^CGG
978 CCGG
MvnI 42 CGCG CG^CG
844 CGCG
NdeII 496 GATC ^GATC
904 GATC
NspV 4 TTCGAA TT^CGAA
1199 TTCGAA
PpuMI 462 AGGTCCC [GA]G^G[AT]CC[CT]
1250 AGGACCT
PstI 877 CTGCAG CTGCA^G
1336 CTGCAG
SacII 41 CCGCGG CCGC^GG
843 CCGCGG
Sau3AI 496 GATC ^GATC
904 GATC
ScaI 484 AGTACT AGT^ACT
1140 AGTACT
SfcI 877 CTGCAG C^T[GA][CT]AG
1336 CTGCAG
SfuI 4 TTCGAA TT^CGAA
1199 TTCGAA
TaqI 5 TCGA T^CGA
1200 TCGA
TthHB8I 5 TCGA T^CGA
1200 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaII
BanII BbvI BfaI BglI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIII NlaIV PflMI PleI RsaI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI Van91I