Restriction map for pF1KA0409
 (Linear) (Six-base) MAPSORT of: KIAA0409.seq  Check: 1159  from: 1  to: 1368



KIAA0409.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     401    1165    1327    1368 
   Size:        278     123     764     162      41

  Fragments arranged by size: 

                 764     278     162     123      41
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441     735    1368 
   Size:        441     294     633

  Fragments arranged by size: 

                 633     441     294
 
ApaI G_GGCC'C

Cuts at:      0      56     138     301     747     785    1301    1316    1368 
   Size:         56      82     163     446      38     516      15      52

  Fragments arranged by size: 

                 516     446     163      82      56      52      38      15
            
 
ApoI r'AATT_y

Cuts at:      0     426    1368 
   Size:        426     942
 
AvaI C'yCGr_G

Cuts at:      0     698    1368 
   Size:        698     670
 
AvrII C'CTAG_G

Cuts at:      0    1018    1368 
   Size:       1018     350
 
BanI G'GyrC_C

Cuts at:      0     618    1368 
   Size:        618     750
 
BanII G_rGCy'C

Cuts at:      0      56     103     138     301     720     747     785    1301 
   Size:         56      47      35     163     419      27      38     516

Cuts at:   1301    1316    1368 
   Size:         15      52

  Fragments arranged by size: 

                 516     419     163      56      52      47      38      35
                  27      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     661     821    1368 
   Size:        661     160     547

  Fragments arranged by size: 

                 661     547     160
 
BbvCI CC'TCA_GC

Cuts at:      0     638    1172    1368 
   Size:        638     534     196

  Fragments arranged by size: 

                 638     534     196
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     985    1368 
   Size:        985     383
 
BglI GCCn_nnn'nGGC

Cuts at:      0      28     142     724    1368 
   Size:         28     114     582     644

  Fragments arranged by size: 

                 644     582     114      28
 
BlpI GC'TnA_GC

Cuts at:      0    1322    1368 
   Size:       1322      46
 
Bme1580I G_kGCm'C

Cuts at:      0      56     138     301     747     785    1301    1316    1368 
   Size:         56      82     163     446      38     516      15      52

  Fragments arranged by size: 

                 516     446     163      82      56      52      38      15
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      28    1304    1368 
   Size:         28    1276      64

  Fragments arranged by size: 

                1276      64      28
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1075    1368 
   Size:       1075     293
 
Bpu10I CC'TnA_GC

Cuts at:      0     638    1172    1368 
   Size:        638     534     196

  Fragments arranged by size: 

                 638     534     196
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1162    1368 
   Size:       1162     206
 
BsaWI w'CCGG_w

Cuts at:      0     977    1368 
   Size:        977     391
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      85     115    1048    1078    1368 
   Size:         85      30     933      30     290

  Fragments arranged by size: 

                 933     290      85      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75     208     211     214     217    1141    1368 
   Size:         75     133       3       3       3     924     227

  Fragments arranged by size: 

                 924     227     133      75       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     158     169     434     633     784    1368 
   Size:        158      11     265     199     151     584

  Fragments arranged by size: 

                 584     265     199     158     151      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     779    1368 
   Size:        779     589
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     808    1368 
   Size:        808     560
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     103     138     301     720     747     785    1301 
   Size:         56      47      35     163     419      27      38     516

Cuts at:   1301    1316    1368 
   Size:         15      52

  Fragments arranged by size: 

                 516     419     163      56      52      47      38      35
                  27      15
 
BspEI T'CCGG_A

Cuts at:      0     977    1368 
   Size:        977     391
 
BsrDI GCAATG_nn'

Cuts at:      0     990    1368 
   Size:        990     378
 
BsrFI r'CCGG_y

Cuts at:      0     569    1368 
   Size:        569     799
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     241    1368 
   Size:        241    1127
 
BstBI TT'CG_AA

Cuts at:      0       5    1200    1368 
   Size:          5    1195     168

  Fragments arranged by size: 

                1195     168       5
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1294    1368 
   Size:       1294      74
 
BstYI r'GATC_y

Cuts at:      0     903    1368 
   Size:        903     465
 
Bsu36I CC'TnA_GG

Cuts at:      0     614     821    1368 
   Size:        614     207     547

  Fragments arranged by size: 

                 614     547     207
 
BtgI C'CryG_G

Cuts at:      0      41     843    1368 
   Size:         41     802     525

  Fragments arranged by size: 

                 802     525      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     879    1368 
   Size:        879     489
 
BtsI GCAGTG_nn'

Cuts at:      0     315     375     565     795     874    1368 
   Size:        315      60     190     230      79     494

  Fragments arranged by size: 

                 494     315     230     190      79      60
 
DraIII CAC_nnn'GTG

Cuts at:      0    1031    1368 
   Size:       1031     337
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     663    1021    1368 
   Size:        663     358     347

  Fragments arranged by size: 

                 663     358     347
 
EaeI y'GGCC_r

Cuts at:      0     122     871    1368 
   Size:        122     749     497

  Fragments arranged by size: 

                 749     497     122
 
EarI CTCTTCn'nnn_

Cuts at:      0       2     811    1368 
   Size:          2     809     557

  Fragments arranged by size: 

                 809     557       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      62     579    1368 
   Size:         62     517     789

  Fragments arranged by size: 

                 789     517      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     401    1075    1165    1327    1368 
   Size:        278     123     674      90     162      41

  Fragments arranged by size: 

                 674     278     162     123      90      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1126    1307    1368 
   Size:       1126     181      61

  Fragments arranged by size: 

                1126     181      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     297     463     630     642     743     781    1251 
   Size:        135     162     166     167      12     101      38     470

Cuts at:   1251    1298    1312    1368 
   Size:         47      14      56

  Fragments arranged by size: 

                 470     167     166     162     135     101      56      47
                  38      14      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1248    1368 
   Size:       1248     120
 
HaeII r_GCGC'y

Cuts at:      0     111    1368 
   Size:        111    1257
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165    1048    1080    1368 
   Size:        133      32     883      32     288

  Fragments arranged by size: 

                 883     288     133      32      32
 
HincII GTy'rAC

Cuts at:      0     453    1368 
   Size:        453     915
 
MscI TGG'CCA

Cuts at:      0     124     873    1368 
   Size:        124     749     495

  Fragments arranged by size: 

                 749     495     124
 
MspA1I CmG'CkG

Cuts at:      0      43     637     732     845     915    1368 
   Size:         43     594      95     113      70     453

  Fragments arranged by size: 

                 594     453     113      95      70      43
 
NspI r_CATG'y

Cuts at:      0     418    1368 
   Size:        418     950
 
PasI CC'CwG_GG

Cuts at:      0     514    1368 
   Size:        514     854
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     441     473    1368 
   Size:        120     321      32     895

  Fragments arranged by size: 

                 895     321     120      32
 
PfoI T'CCnGG_A

Cuts at:      0     809    1368 
   Size:        809     559
 
PpuMI rG'GwC_Cy

Cuts at:      0     463    1251    1368 
   Size:        463     788     117

  Fragments arranged by size: 

                 788     463     117
 
PspOMI G'GGCC_C

Cuts at:      0      52     134     297     743     781    1297    1312    1368 
   Size:         52      82     163     446      38     516      15      56

  Fragments arranged by size: 

                 516     446     163      82      56      52      38      15
            
 
PstI C_TGCA'G

Cuts at:      0     881    1340    1368 
   Size:        881     459      28

  Fragments arranged by size: 

                 881     459      28
 
PvuII CAG'CTG

Cuts at:      0     637    1368 
   Size:        637     731
 
SacII CC_GC'GG

Cuts at:      0      44     846    1368 
   Size:         44     802     522

  Fragments arranged by size: 

                 802     522      44
 
SapI GCTCTTCn'nnn_

Cuts at:      0       2    1368 
   Size:          2    1366
 
ScaI AGT'ACT

Cuts at:      0     486    1142    1368 
   Size:        486     656     226

  Fragments arranged by size: 

                 656     486     226
 
SfcI C'TryA_G

Cuts at:      0     877    1336    1368 
   Size:        877     459      32

  Fragments arranged by size: 

                 877     459      32
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      28    1368 
   Size:         28    1340
 
StuI AGG'CCT

Cuts at:      0    1334    1368 
   Size:       1334      34
 
StyI C'CwwG_G

Cuts at:      0      48    1018    1247    1316    1368 
   Size:         48     970     229      69      52

  Fragments arranged by size: 

                 970     229      69      52      48
 
TatI w'GTAC_w

Cuts at:      0     484     773    1140    1368 
   Size:        484     289     367     228

  Fragments arranged by size: 

                 484     367     289     228
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI    AvrII     BanI    BanII
     BbsI    BbvCI    BciVI     BglI     BlpI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsmBI
 Bsp1286I    BspEI    BsrDI    BsrFI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
     MscI   MspA1I     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII    SacII     SapI     ScaI     SfcI     SfiI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     BaeI    BamHI     BbeI     BcgI     BclI    BfrBI    BglII
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstEII  BstZ17I     ClaI    CspCI     DraI     EagI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SbfI    SexAI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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