Restriction map for pF1KA0354
 (Circular) (Six-base) MAPSORT of: pF1KA0354.seq  Check: 7608  from: 1  to: 5144



pF1KA0354  5144 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    431    1462     431 
   Size:       1031    4113
 
AccI GT'mk_AC

Cuts at:    361    2182     361 
   Size:       1821    3323
 
Acc65I G'GTAC_C

Cuts at:   2160    2160 
   Size:       5144
 
AclI AA'CG_TT

Cuts at:   4984    4984 
   Size:       5144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    761    1571    2059    2969    3401    4148    4416     761 
   Size:        810     488     910     432     747     268    1489

  Fragments arranged by size: 

                1489     910     810     747     488     432     268
 
AfeI AGC'GCT

Cuts at:    532     532 
   Size:       5144
 
AflIII A'CryG_T

Cuts at:   2496    3600    4577    2496 
   Size:       1104     977    3063

  Fragments arranged by size: 

                3063    1104     977
 
AgeI A'CCGG_T

Cuts at:   3532    3532 
   Size:       5144
 
AleI CACnn'nnGTG

Cuts at:   2003    2015    2003 
   Size:         12    5132
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    122     154     122 
   Size:         32    5112
 
AlwNI CAG_nnn'CTG

Cuts at:    147     552    1462    2449    4016     147 
   Size:        405     910     987    1567    1275

  Fragments arranged by size: 

                1567    1275     987     910     405
 
ApaI G_GGCC'C

Cuts at:   1481    1481 
   Size:       5144
 
ApaLI G'TGCA_C

Cuts at:   3914    3914 
   Size:       5144
 
ApoI r'AATT_y

Cuts at:   1338    2148    4497    4753    1338 
   Size:        810    2349     256    1729

  Fragments arranged by size: 

                2349    1729     810     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5144
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5144
 
AvaI C'yCGr_G

Cuts at:    791    2164     791 
   Size:       1373    3771
 
BamHI G'GATC_C

Cuts at:   2169    2169 
   Size:       5144
 
BanI G'GyrC_C

Cuts at:    921    2160    2574    2822    2857     921 
   Size:       1239     414     248      35    3208

  Fragments arranged by size: 

                3208    1239     414     248      35
 
BanII G_rGCy'C

Cuts at:    261     574     625    1114    1481    1759    1938    2158    3188 
   Size:        313      51     489     367     278     179     220    1030

Cuts at:   3188     261 
   Size:       2217

  Fragments arranged by size: 

                2217    1030     489     367     313     278     220     179
                  51
 
BbeI G_GCGC'C

Cuts at:   2578    2826    2578 
   Size:        248    4896
 
BbvCI CC'TCA_GC

Cuts at:   1216    1216 
   Size:       5144
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2118    2152    2118 
   Size:         34    5110
 
BciVI GTATCCnnnnn_n'

Cuts at:   3042    3803    5144    3042 
   Size:        761    1341    3042

  Fragments arranged by size: 

                3042    1341     761
 
BclI T'GATC_A

Cuts at:    888    1005    2664     888 
   Size:        117    1659    3368

  Fragments arranged by size: 

                3368    1659     117
 
BglI GCCn_nnn'nGGC

Cuts at:    596     596 
   Size:       5144
 
BglII A'GATC_T

Cuts at:   2659    4696    2659 
   Size:       2037    3107
 
BlpI GC'TnA_GC

Cuts at:    573    2256     573 
   Size:       1683    3461
 
Bme1580I G_kGCm'C

Cuts at:    926    1481    2769    2862    3918     926 
   Size:        555    1288      93    1056    2152

  Fragments arranged by size: 

                2152    1288    1056     555      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    488    1248    2356    2437    2772    4916     488 
   Size:        760    1108      81     335    2144     716

  Fragments arranged by size: 

                2144    1108     760     716     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3511    3511 
   Size:       5144
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1447    1644    1447 
   Size:        197    4947
 
Bpu10I CC'TnA_GC

Cuts at:   1118    1216    1118 
   Size:         98    5046
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    890    2320    3199    3691    3989     890 
   Size:       1430     879     492     298    2045

  Fragments arranged by size: 

                2045    1430     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4848    4848 
   Size:       5144
 
BsaAI yAC'GTr

Cuts at:   2497    2497 
   Size:       5144
 
BsaBI GATnn'nnATC

Cuts at:      6    2658    4695       6 
   Size:       2652    2037     455

  Fragments arranged by size: 

                2652    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    672    2575    2823    3525    5021     672 
   Size:       1903     248     702    1496     795

  Fragments arranged by size: 

                1903    1496     795     702     248
 
BsaWI w'CCGG_w

Cuts at:    762    2333    2854    3532    3806    3953    4364     762 
   Size:       1571     521     678     274     147     411    1542

  Fragments arranged by size: 

                1571    1542     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1178 
   Size:         30    5114
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    616     715     811    1473    1616    1641    1703     616 
   Size:         99      96     662     143      25      62    4057

  Fragments arranged by size: 

                4057     662     143      99      96      62      25
 
BseYI C'CCAG_C

Cuts at:    378     552     925    1462    1838    2125    2569    3327    3904 
   Size:        174     373     537     376     287     444     758     577

Cuts at:   3904    4400     378 
   Size:        496    1122

  Fragments arranged by size: 

                1122     758     577     537     496     444     376     373
                 287     174
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    326    1001    1600     326 
   Size:        675     599    3870

  Fragments arranged by size: 

                3870     675     599
 
BsiEI CG_ry'CG

Cuts at:    104    2686    2732    3940     104 
   Size:       2582      46    1208    1308

  Fragments arranged by size: 

                2582    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    574     625    1079    1114    1759    1913    1938    2158    2936 
   Size:         51     454      35     645     154      25     220     778

Cuts at:   2936    3126    3918     574 
   Size:        190     792    1800

  Fragments arranged by size: 

                1800     792     778     645     454     220     190     154
                  51      35      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:    663     663 
   Size:       5144
 
Bsp1286I G_dGCh'C

Cuts at:    261     574     625     926    1079    1114    1481    1759    1913 
   Size:        313      51     301     153      35     367     278     154

Cuts at:   1913    1938    2158    2769    2862    2936    3126    3188    3918 
   Size:         25     220     611      93      74     190      62     730

Cuts at:   3918     261 
   Size:       1487

  Fragments arranged by size: 

                1487     730     611     367     313     301     278     220
                 190     154     153      93      74      62      51      35
                  25
 
BspEI T'CCGG_A

Cuts at:   4364    4364 
   Size:       5144
 
BspHI T'CATG_A

Cuts at:   4320    4320 
   Size:       5144
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    431    1462    1533    2104    2194    2408    2710    3091     431 
   Size:       1031      71     571      90     214     302     381    2484

  Fragments arranged by size: 

                2484    1031     571     381     302     214      90      71
            
 
BsrBI CCG'CTC

Cuts at:     50     224     705    3436    3490    4974    5139      50 
   Size:        174     481    2731      54    1484     165      55

  Fragments arranged by size: 

                2731    1484     481     174     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    396    3056     396 
   Size:       2660    2484
 
BsrFI r'CCGG_y

Cuts at:   3142    3323    3532    3142 
   Size:        181     209    4754

  Fragments arranged by size: 

                4754     209     181
 
BssSI C'ACGA_G

Cuts at:    977    3415    3773     977 
   Size:       2438     358    2348

  Fragments arranged by size: 

                2438    2348     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1958    2521    1958 
   Size:        563    4581
 
BstBI TT'CG_AA

Cuts at:   1336    3505    1336 
   Size:       2169    2975
 
BstYI r'GATC_y

Cuts at:      1    1248    1763    2169    2636    2659    2994    3240    4252 
   Size:       1247     515     406     467      23     335     246    1012

Cuts at:   4252    4338    4350    4696       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1247    1012     515     467     449     406     346     335
                 246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    362     362 
   Size:       5144
 
Bsu36I CC'TnA_GG

Cuts at:   1052    1124    1052 
   Size:         72    5072
 
BtgI C'CryG_G

Cuts at:    107     968     107 
   Size:        861    4283
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1154    3094    3275    3438    1154 
   Size:       1940     181     163    2860

  Fragments arranged by size: 

                2860    1940     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1236    2387    1236 
   Size:       1151    3993
 
DraI TTT'AAA

Cuts at:    213    2143     213 
   Size:       1930    3214
 
DrdI GACnn_nn'nnGTC

Cuts at:   2850    3708    2850 
   Size:        858    4286
 
EaeI y'GGCC_r

Cuts at:    168    1827    2729    2903    3294    3321    4666     168 
   Size:       1659     902     174     391      27    1345     646

  Fragments arranged by size: 

                1659    1345     902     646     391     174      27
 
EagI C'GGCC_G

Cuts at:   2729    2729 
   Size:       5144
 
EarI CTCTTCn'nnn_

Cuts at:    630    3167    3377     630 
   Size:       2537     210    2397

  Fragments arranged by size: 

                2537    2397     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3352    3662    3808    4954    3352 
   Size:        310     146    1146    3542

  Fragments arranged by size: 

                3542    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    572     623    1112    1757    1936    2156     572 
   Size:         51     489     645     179     220    3560

  Fragments arranged by size: 

                3560     645     489     220     179      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    761    1447    1571    1644    2059    2969    3401    4148    4416 
   Size:        686     124      73     415     910     432     747     268

Cuts at:   4416     761 
   Size:       1489

  Fragments arranged by size: 

                1489     910     747     686     432     415     268     124
                  73
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1659    1659 
   Size:       5144
 
EcoO109I rG'GnC_Cy

Cuts at:   1437    1478    2283    1437 
   Size:         41     805    4298

  Fragments arranged by size: 

                4298     805      41
 
EcoRI G'AATT_C

Cuts at:   2148    2148 
   Size:       5144
 
EcoRV GAT'ATC

Cuts at:   1281    1281 
   Size:       5144
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1917    3163    1917 
   Size:       1246    3898
 
FspI TGC'GCA

Cuts at:    634    2925     634 
   Size:       2291    2853
 
HaeII r_GCGC'y

Cuts at:    521     534     720    2578    2826    3848    4827     521 
   Size:         13     186    1858     248    1022     979     838

  Fragments arranged by size: 

                1858    1022     979     838     248     186      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    548     580    1132    1164    1178    1210    1240    1272    1303 
   Size:         32     552      32      14      32      30      32      31

Cuts at:   1303    1335    1867    1899    2043    2075    2103    2135    2986 
   Size:         32     532      32     144      32      28      32     851

Cuts at:   2986    3016    3018    3048     548 
   Size:         30       2      30    2644

  Fragments arranged by size: 

                2644     851     552     532     144      32      32      32
                  32      32      32      32      32      31      30      30
                  30      28      14       2
 
HincII GTy'rAC

Cuts at:   1859    2183    1859 
   Size:        324    4820
 
KasI G'GCGC_C

Cuts at:   2574    2822    2574 
   Size:        248    4896
 
KpnI G_GTAC'C

Cuts at:   2164    2164 
   Size:       5144
 
MluI A'CGCG_T

Cuts at:   4577    4577 
   Size:       5144
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    747    3815    3999     747 
   Size:       3068     184    1892

  Fragments arranged by size: 

                3068    1892     184
 
MscI TGG'CCA

Cuts at:    170    1829    2905     170 
   Size:       1659    1076    2409

  Fragments arranged by size: 

                2409    1659    1076
 
MslI CAynn'nnrTG

Cuts at:    193     295     373    1084    2003    2015    3133    3260     193 
   Size:        102      78     711     919      12    1118     127    2077

  Fragments arranged by size: 

                2077    1118     919     711     127     102      78      12
            
 
MspA1I CmG'CkG

Cuts at:    147     306     552    2255    2569    2929    3942    4187     147 
   Size:        159     246    1703     314     360    1013     245    1104

  Fragments arranged by size: 

                1703    1104    1013     360     314     246     245     159
            
 
NaeI GCC'GGC

Cuts at:   3325    3325 
   Size:       5144
 
NarI GG'CG_CC

Cuts at:   2575    2823    2575 
   Size:        248    4896
 
NcoI C'CATG_G

Cuts at:    107     968     107 
   Size:        861    4283
 
NgoMIV G'CCGG_C

Cuts at:   3323    3323 
   Size:       5144
 
NspI r_CATG'y

Cuts at:    242     452     528    2197    2697    3604     242 
   Size:        210      76    1669     500     907    1782

  Fragments arranged by size: 

                1782    1669     907     500     210      76
 
PciI A'CATG_T

Cuts at:   3600    3600 
   Size:       5144
 
PflMI CCAn_nnn'nTGG

Cuts at:   1290    1462    1290 
   Size:        172    4972
 
PmeI GTTT'AAAC

Cuts at:   2143    2143 
   Size:       5144
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4283    4315    4283 
   Size:         32    5112
 
PspOMI G'GGCC_C

Cuts at:   1477    1477 
   Size:       5144
 
PstI C_TGCA'G

Cuts at:    699    1423    1473    1530    1875    2191    2876     699 
   Size:        724      50      57     345     316     685    2967

  Fragments arranged by size: 

                2967     724     685     345     316      57      50
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5144
 
PvuII CAG'CTG

Cuts at:    147     306     552    2569    2929     147 
   Size:        159     246    2017     360    2362

  Fragments arranged by size: 

                2362    2017     360     246     159
 
RsrII CG'GwC_CG

Cuts at:   3339    3339 
   Size:       5144
 
SacI G_AGCT'C

Cuts at:    574     625    1114    1759    1938    2158     574 
   Size:         51     489     645     179     220    3560

  Fragments arranged by size: 

                3560     645     489     220     179      51
 
SalI G'TCGA_C

Cuts at:   2181    2181 
   Size:       5144
 
SapI GCTCTTCn'nnn_

Cuts at:    630    3167    3377     630 
   Size:       2537     210    2397

  Fragments arranged by size: 

                2537    2397     210
 
SbfI CC_TGCA'GG

Cuts at:   1473    2191    1473 
   Size:        718    4426
 
ScaI AGT'ACT

Cuts at:   4661    4661 
   Size:       5144
 
SfcI C'TryA_G

Cuts at:     33     544     695    1419    1469    1526    1871    2187    2872 
   Size:        511     151     724      50      57     345     316     685

Cuts at:   2872    3865    4056      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     724     685     511     345     316     191
                 151      57      50
 
SfoI GGC'GCC

Cuts at:   2576    2824    2576 
   Size:        248    4896
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5144
 
SmaI CCC'GGG

Cuts at:   2166    2166 
   Size:       5144
 
SmlI C'TyrA_G

Cuts at:    905    2299    3214    3706    3968     905 
   Size:       1394     915     492     262    2081

  Fragments arranged by size: 

                2081    1394     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     997    1411      67 
   Size:        930     414    3800

  Fragments arranged by size: 

                3800     930     414
 
SphI G_CATG'C

Cuts at:    242     528    2197    2697     242 
   Size:        286    1669     500    2689

  Fragments arranged by size: 

                2689    1669     500     286
 
SspI AAT'ATT

Cuts at:   1398    1398 
   Size:       5144
 
StuI AGG'CCT

Cuts at:    334    1548    1900     334 
   Size:       1214     352    3578

  Fragments arranged by size: 

                3578    1214     352
 
StyI C'CwwG_G

Cuts at:    107     968    1062    1596    1681    2278    2632     107 
   Size:        861      94     534      85     597     354    2619

  Fragments arranged by size: 

                2619     861     597     534     354      94      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2727    2860    3319    3528    2727 
   Size:        133     459     209    4343

  Fragments arranged by size: 

                4343     459     209     133
 
TatI w'GTAC_w

Cuts at:   4659    4659 
   Size:       5144
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4661    4693    4661 
   Size:         32    5112
 
Tth111I GACn'n_nGTC

Cuts at:   2941    2941 
   Size:       5144
 
XbaI T'CTAG_A

Cuts at:   2175    2175 
   Size:       5144
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    163     375    1635    2639     163 
   Size:        212    1260    1004    2668

  Fragments arranged by size: 

                2668    1260    1004     212
 
XmaI C'CCGG_G

Cuts at:   2164    2164 
   Size:       5144
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AlfI     AscI    AvrII     BaeI     BbsI
    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsrGI   BssHII   BstEII
    BstXI     ClaI    CspCI   DraIII     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI
     XmnI     ZraI
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