Restriction map for pF1KA0339
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AccI        AclI        AflII       AgeI        AhdI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        BbrPI       BclI        
BfrI        BsmI        Bsp1407I    BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      DraI        Eam1105I    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        KspI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        ScaI        SfuI        SgrAI       SpeI        
SphI        SspBI       SspI        SwaI        Tth111I     
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        2804  GACGTC               GACGT^C
Acc65I       4642  GGTACC               G^GTACC
AcsI         2705  AAATTT               [GA]^AATT[CT]
ApoI         2705  AAATTT               [GA]^AATT[CT]
Asp700I      1360  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4642  GGTACC               G^GTACC
AviII          95  TGCGCA               TGC^GCA
AvrII        3865  CCTAGG               C^CTAGG
BcgI         4586  CGAACCGCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2982  GGATAC               GTATCC(6/5)
BfaI         3866  CTAG                 C^TAG
BglII        1751  AGATCT               A^GATCT
BlnI         3865  CCTAGG               C^CTAGG
BspDI        4897  ATCGAT               AT^CGAT
BsrDI        4761  CATTGC               GCAATG(2/0)
ClaI         4897  ATCGAT               AT^CGAT
CpoI         4609  CGGTCCG              CG^G[AT]CCG
EagI          638  CGGCCG               C^GGCCG
EclXI         638  CGGCCG               C^GGCCG
Eco52I        638  CGGCCG               C^GGCCG
FspI           95  TGCGCA               TGC^GCA
HindIII       741  AAGCTT               A^AGCTT
HpaI         2058  GTTAAC               GTT^AAC
KpnI         4642  GGTACC               GGTAC^C
MaeI         3866  CTAG                 C^TAG
NaeI         1253  GCCGGC               GCC^GGC
NcoI         4751  CCATGG               C^CATGG
NgoMIV       1253  GCCGGC               G^CCGGC
NspI         4823  ACATGC               [GA]CATG^[CT]
PpuMI        4001  AGGTCCT              [GA]G^G[AT]CC[CT]
RsrII        4609  CGGTCCG              CG^G[AT]CCG
SacI         1990  GAGCTC               GAGCT^C
SbfI         3352  CCTGCAGG             CCTGCA^GG
SexAI        1317  ACCTGGT              A^CC[AT]GGT
SfiI         3768  GGCCGACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         4687  CTCAAG               C^T[CT][GA]AG
SnaBI        4335  TACGTA               TAC^GTA
Sse8387I     3352  CCTGCAGG             CCTGCA^GG
XmnI         1360  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccIII       4166  TCCGGA               T^CCGGA
             4694  TCCGGA              
AflIII        818  ACACGT               A^C[GA][CT]GT
             4535  ACGTGT              
BalI          780  TGGCCA               TGG^CCA
             4922  TGGCCA              
BamHI          72  GGATCC               G^GATCC
             4727  GGATCC              
BsaWI        4166  TCCGGA               [AT]^CCGG[AT]
             4694  TCCGGA              
BseAI        4166  TCCGGA               T^CCGGA
             4694  TCCGGA              
BsiEI         638  CGGCCG               CG[GA][CT]^CG
             1764  CGACCG              
BsiWI         388  CGTACG               C^GTACG
             4337  CGTACG              
BspEI        4166  TCCGGA               T^CCGGA
             4694  TCCGGA              
BssSI        4732  CACGAG               CACGAG(-5/-1)
             2587  CTCGTG              
DraIII       4250  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4905  CACCAAGTG           
EcoNI        1318  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3714  CCTCACCGAGG         
HincII        145  GTCAAC               GT[CT]^[GA]AC
             2058  GTTAAC              
HindII        145  GTCAAC               GT[CT]^[GA]AC
             2058  GTTAAC              
MluNI         780  TGGCCA               TGG^CCA
             4922  TGGCCA              
MroI         4166  TCCGGA               T^CCGGA
             4694  TCCGGA              
MscI          780  TGGCCA               TGG^CCA
             4922  TGGCCA              
MseI          242  TTAA                 T^TAA
             2059  TTAA                
PflMI        1312  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3406  CCACCTGCTGG         
Tru9I         242  TTAA                 T^TAA
             2059  TTAA                
Van91I       1312  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3406  CCACCTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        Eco47III    EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmaCI       XmaI        
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