Restriction map for pF1KA0321
 (Linear) (Six-base) MAPSORT of: KIAA0321.seq  Check: 8129  from: 1  to: 7617



KIAA0321.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4545    7497    7617 
   Size:       4545    2952     120

  Fragments arranged by size: 

                4545    2952     120
 
AccI GT'mk_AC

Cuts at:      0    3021    5331    7617 
   Size:       3021    2310    2286

  Fragments arranged by size: 

                3021    2310    2286
 
Acc65I G'GTAC_C

Cuts at:      0    5427    7617 
   Size:       5427    2190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145    2593    3064    3178    3256    3450    4273    4319 
   Size:        145    2448     471     114      78     194     823      46

Cuts at:   4319    5621    6289    6470    6807    6916    6936    6985    7617 
   Size:       1302     668     181     337     109      20      49     632

  Fragments arranged by size: 

                2448    1302     823     668     632     471     337     194
                 181     145     114     109      78      49      46      20
            
 
AfeI AGC'GCT

Cuts at:      0    2614    2742    7617 
   Size:       2614     128    4875

  Fragments arranged by size: 

                4875    2614     128
 
AflII C'TTAA_G

Cuts at:      0    3922    6877    7617 
   Size:       3922    2955     740

  Fragments arranged by size: 

                3922    2955     740
 
AflIII A'CryG_T

Cuts at:      0    2281    7617 
   Size:       2281    5336
 
AhdI GACnn_n'nnGTC

Cuts at:      0     440    3122    7617 
   Size:        440    2682    4495

  Fragments arranged by size: 

                4495    2682     440
 
AleI CACnn'nnGTG

Cuts at:      0    4300    7617 
   Size:       4300    3317
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4048    6113    7147    7617 
   Size:       4048    2065    1034     470

  Fragments arranged by size: 

                4048    2065    1034     470
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2587    2619    7617 
   Size:       2587      32    4998

  Fragments arranged by size: 

                4998    2587      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     510    1014    1167    1456    1482    1642    1686    2556 
   Size:        510     504     153     289      26     160      44     870

Cuts at:   2556    3279    3473    3689    4158    4956    5109    5112    5224 
   Size:        723     194     216     469     798     153       3     112

Cuts at:   5224    5534    5946    6263    7272    7374    7617 
   Size:        310     412     317    1009     102     243

  Fragments arranged by size: 

                1009     870     798     723     510     504     469     412
                 317     310     289     243     216     194     160     153
                 153     112     102      44      26       3
 
ApaI G_GGCC'C

Cuts at:      0     619    1186    4287    7617 
   Size:        619     567    3101    3330

  Fragments arranged by size: 

                3330    3101     619     567
 
ApoI r'AATT_y

Cuts at:      0    1081    2044    5386    5773    7358    7617 
   Size:       1081     963    3342     387    1585     259

  Fragments arranged by size: 

                3342    1585    1081     963     387     259
 
AvaI C'yCGr_G

Cuts at:      0     263     358     876    1003    3825    3972    4058    4280 
   Size:        263      95     518     127    2822     147      86     222

Cuts at:   4280    5266    7586    7617 
   Size:        986    2320      31

  Fragments arranged by size: 

                2822    2320     986     518     263     222     147     127
                  95      86      31
 
BamHI G'GATC_C

Cuts at:      0    4966    7617 
   Size:       4966    2651
 
BanI G'GyrC_C

Cuts at:      0     627    3457    4032    5417    5427    7591    7617 
   Size:        627    2830     575    1385      10    2164      26

  Fragments arranged by size: 

                2830    2164    1385     627     575      26      10
 
BanII G_rGCy'C

Cuts at:      0     521     619     663    1186    1202    1233    1828    1858 
   Size:        521      98      44     523      16      31     595      30

Cuts at:   1858    2867    3308    3514    3574    3701    3823    4127    4287 
   Size:       1009     441     206      60     127     122     304     160

Cuts at:   4287    7617 
   Size:       3330

  Fragments arranged by size: 

                3330    1009     595     523     521     441     304     206
                 160     127     122      98      60      44      31      30
                  16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     158     332    1052    2900    3317    7617 
   Size:        158     174     720    1848     417    4300

  Fragments arranged by size: 

                4300    1848     720     417     174     158
 
BbvCI CC'TCA_GC

Cuts at:      0     110    1599    1799    1859    7617 
   Size:        110    1489     200      60    5758

  Fragments arranged by size: 

                5758    1489     200     110      60
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    6367    6401    7617 
   Size:       6367      34    1216

  Fragments arranged by size: 

                6367    1216      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    6372    7617 
   Size:       6372    1245
 
BclI T'GATC_A

Cuts at:      0    3522    5592    7617 
   Size:       3522    2070    2025

  Fragments arranged by size: 

                3522    2070    2025
 
BfrBI ATG'CAT

Cuts at:      0    4411    7617 
   Size:       4411    3206
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1983    3640    5086    5513    7617 
   Size:       1983    1657    1446     427    2104

  Fragments arranged by size: 

                2104    1983    1657    1446     427
 
BglII A'GATC_T

Cuts at:      0    1290    6083    6899    7617 
   Size:       1290    4793     816     718

  Fragments arranged by size: 

                4793    1290     816     718
 
BlpI GC'TnA_GC

Cuts at:      0     778    2383    7617 
   Size:        778    1605    5234

  Fragments arranged by size: 

                5234    1605     778
 
Bme1580I G_kGCm'C

Cuts at:      0     619     632    1186    3460    4287    5396    7563    7596 
   Size:        619      13     554    2274     827    1109    2167      33

Cuts at:   7596    7617 
   Size:         21

  Fragments arranged by size: 

                2274    2167    1109     827     619     554      33      21
                  13
 
BmgBI CAC'GTC

Cuts at:      0    2284    2781    7617 
   Size:       2284     497    4836

  Fragments arranged by size: 

                4836    2284     497
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277    1551    2202    2285    2579    4585    6157    6182 
   Size:        277    1274     651      83     294    2006    1572      25

Cuts at:   6182    6862    7557    7617 
   Size:        680     695      60

  Fragments arranged by size: 

                2006    1572    1274     695     680     651     294     277
                  83      60      25
 
BmtI G_CTAG'C

Cuts at:      0     925    7617 
   Size:        925    6692
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     452     506    5169    6449    6880    6882    7617 
   Size:        452      54    4663    1280     431       2     735

  Fragments arranged by size: 

                4663    1280     735     452     431      54       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     535    1144    1258    1267    1433    1735    2812 
   Size:        268     267     609     114       9     166     302    1077

Cuts at:   2812    2828    3341    3385    4480    4492    5065    5086    5310 
   Size:         16     513      44    1095      12     573      21     224

Cuts at:   5310    6581    7396    7593    7617 
   Size:       1271     815     197      24

  Fragments arranged by size: 

                1271    1095    1077     815     609     573     513     302
                 268     267     224     197     166     114      44      24
                  21      16      12       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     110     908     982    1457    1465    1599    1799    1859 
   Size:        110     798      74     475       8     134     200      60

Cuts at:   1859    2145    3214    4351    4991    6112    7617 
   Size:        286    1069    1137     640    1121    1505

  Fragments arranged by size: 

                1505    1137    1121    1069     798     640     475     286
                 200     134     110      74      60       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     547    1372    4635    6291    7617 
   Size:        325     222     825    3263    1656    1326

  Fragments arranged by size: 

                3263    1656    1326     825     325     222
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     367    2804    5263    6165    7617 
   Size:        367    2437    2459     902    1452

  Fragments arranged by size: 

                2459    2437    1452     902     367
 
BsaAI yAC'GTr

Cuts at:      0     402    3036    6066    6540    7617 
   Size:        402    2634    3030     474    1077

  Fragments arranged by size: 

                3030    2634    1077     474     402
 
BsaBI GATnn'nnATC

Cuts at:      0    5286    7617 
   Size:       5286    2331
 
BsaHI Gr'CG_yC

Cuts at:      0    5493    7617 
   Size:       5493    2124
 
BsaWI w'CCGG_w

Cuts at:      0     293     891    5430    7617 
   Size:        293     598    4539    2187

  Fragments arranged by size: 

                4539    2187     598     293
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271    1537    1567    1612    1642    4611    4641 
   Size:        241      30    1266      30      45      30    2969      30

Cuts at:   4641    7617 
   Size:       2976

  Fragments arranged by size: 

                2976    2969    1266     241      45      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     376     428     544     781    1192    1620    2263    3173 
   Size:        376      52     116     237     411     428     643     910

Cuts at:   3173    3305    3388    3729    3858    3894    4632    5303    5644 
   Size:        132      83     341     129      36     738     671     341

Cuts at:   5644    5761    7321    7617 
   Size:        117    1560     296

  Fragments arranged by size: 

                1560     910     738     671     643     428     411     376
                 341     341     296     237     132     129     117     116
                  83      52      36
 
BseYI C'CCAG_C

Cuts at:      0     494     945    1421    3208    3251    3770    3891    3994 
   Size:        494     451     476    1787      43     519     121     103

Cuts at:   3994    5372    5397    5794    6698    7048    7134    7617 
   Size:       1378      25     397     904     350      86     483

  Fragments arranged by size: 

                1787    1378     904     519     494     483     476     451
                 397     350     121     103      86      43      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     775    1153    1710    2422    3195    3400    5113 
   Size:        410     365     378     557     712     773     205    1713

Cuts at:   5113    5547    5812    6227    6337    6484    6556    7531    7617 
   Size:        434     265     415     110     147      72     975      86

  Fragments arranged by size: 

                1713     975     773     712     557     434     415     410
                 378     365     265     205     147     110      86      72
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     470     521    1202    1334    1463    2389    2654    3514 
   Size:        470      51     681     132     129     926     265     860

Cuts at:   3514    3574    3823    5474    5870    7617 
   Size:         60     249    1651     396    1747

  Fragments arranged by size: 

                1747    1651     926     860     681     470     396     265
                 249     132     129      60      51
 
BsmI GAATG_Cn'

Cuts at:      0      70     576    3056    6490    7617 
   Size:         70     506    2480    3434    1127

  Fragments arranged by size: 

                3434    2480    1127     506      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     448    7617 
   Size:        448    7169
 
Bsp1286I G_dGCh'C

Cuts at:      0     470     521     619     632     663    1186    1202    1233 
   Size:        470      51      98      13      31     523      16      31

Cuts at:   1233    1334    1463    1828    1858    2389    2654    2867    3308 
   Size:        101     129     365      30     531     265     213     441

Cuts at:   3308    3460    3514    3574    3701    3823    4127    4287    5396 
   Size:        152      54      60     127     122     304     160    1109

Cuts at:   5396    5474    5870    7563    7596    7617 
   Size:         78     396    1693      33      21

  Fragments arranged by size: 

                1693    1109     531     523     470     441     396     365
                 304     265     213     160     152     129     127     122
                 101      98      78      60      54      51      33      31
                  31      30      21      16      13
 
BspEI T'CCGG_A

Cuts at:      0     293    7617 
   Size:        293    7324
 
BspHI T'CATG_A

Cuts at:      0    4649    4779    5933    6461    7617 
   Size:       4649     130    1154     528    1156

  Fragments arranged by size: 

                4649    1156    1154     528     130
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2968    4041    4545    6506    7497    7617 
   Size:       2968    1073     504    1961     991     120

  Fragments arranged by size: 

                2968    1961    1073     991     504     120
 
BsrBI CCG'CTC

Cuts at:      0     771     912    2717    7617 
   Size:        771     141    1805    4900

  Fragments arranged by size: 

                4900    1805     771     141
 
BsrDI GCAATG_nn'

Cuts at:      0     598     923    1738    4220    5810    5840    7617 
   Size:        598     325     815    2482    1590      30    1777

  Fragments arranged by size: 

                2482    1777    1590     815     598     325      30
 
BsrFI r'CCGG_y

Cuts at:      0    5516    5977    7617 
   Size:       5516     461    1640

  Fragments arranged by size: 

                5516    1640     461
 
BssSI C'ACGA_G

Cuts at:      0    5582    7617 
   Size:       5582    2035
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1444    4800    5459    5534    6215    7617 
   Size:       1444    3356     659      75     681    1402

  Fragments arranged by size: 

                3356    1444    1402     681     659      75
 
BstBI TT'CG_AA

Cuts at:      0     591    7617 
   Size:        591    7026
 
BstEII G'GTnAC_C

Cuts at:      0    5851    7617 
   Size:       5851    1766
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2332    4915    7617 
   Size:       2332    2583    2702

  Fragments arranged by size: 

                2702    2583    2332
 
BstYI r'GATC_y

Cuts at:      0     474     903    1290    3383    4966    5262    5736    6083 
   Size:        474     429     387    2093    1583     296     474     347

Cuts at:   6083    6899    7262    7617 
   Size:        816     363     355

  Fragments arranged by size: 

                2093    1583     816     474     474     429     387     363
                 355     347     296
 
BstZ17I GTA'TAC

Cuts at:      0    5332    7617 
   Size:       5332    2285
 
Bsu36I CC'TnA_GG

Cuts at:      0     481    1204    1369    1787    1830    2065    6394    7617 
   Size:        481     723     165     418      43     235    4329    1223

  Fragments arranged by size: 

                4329    1223     723     481     418     235     165      43
            
 
BtgI C'CryG_G

Cuts at:      0    2912    3169    7596    7617 
   Size:       2912     257    4427      21

  Fragments arranged by size: 

                4427    2912     257      21
 
BtsI GCAGTG_nn'

Cuts at:      0    1125    2503    2715    2867    3786    4169    5155    5868 
   Size:       1125    1378     212     152     919     383     986     713

Cuts at:   5868    6236    6446    7617 
   Size:        368     210    1171

  Fragments arranged by size: 

                1378    1171    1125     986     919     713     383     368
                 212     210     152
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    7479    7514    7617 
   Size:       7479      35     103

  Fragments arranged by size: 

                7479     103      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     388    1329    2223    3378    4826    7617 
   Size:        388     941     894    1155    1448    2791

  Fragments arranged by size: 

                2791    1448    1155     941     894     388
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    4504    7617 
   Size:       4504    3113
 
EaeI y'GGCC_r

Cuts at:      0     825    1667    2334    2424    2712    4020    4265    4871 
   Size:        825     842     667      90     288    1308     245     606

Cuts at:   4871    4889    5514    5979    6521    7617 
   Size:         18     625     465     542    1096

  Fragments arranged by size: 

                1308    1096     842     825     667     625     606     542
                 465     288     245      90      18
 
EarI CTCTTCn'nnn_

Cuts at:      0    1657    1816    3579    4685    4767    6496    6572    7617 
   Size:       1657     159    1763    1106      82    1729      76    1045

  Fragments arranged by size: 

                1763    1729    1657    1106    1045     159      82      76
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2287    4561    7352    7617 
   Size:       2287    2274    2791     265

  Fragments arranged by size: 

                2791    2287    2274     265
 
EcoICRI GAG'CTC

Cuts at:      0     519    1200    3512    3572    3821    7617 
   Size:        519     681    2312      60     249    3796

  Fragments arranged by size: 

                3796    2312     681     519     249      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     268     535    1144    1258    1267    1433    1735 
   Size:        145     123     267     609     114       9     166     302

Cuts at:   1735    2593    2812    2828    3064    3178    3256    3341    3385 
   Size:        858     219      16     236     114      78      85      44

Cuts at:   3385    3450    4273    4319    4480    4492    5065    5086    5310 
   Size:         65     823      46     161      12     573      21     224

Cuts at:   5310    5621    6289    6470    6581    6807    6916    6936    6985 
   Size:        311     668     181     111     226     109      20      49

Cuts at:   6985    7396    7593    7617 
   Size:        411     197      24

  Fragments arranged by size: 

                 858     823     668     609     573     411     311     302
                 267     236     226     224     219     197     181     166
                 161     145     123     114     114     111     109      85
                  78      65      49      46      44      24      21      20
                  16      12       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     526     718     763    1372    1585    2064    2881    3217 
   Size:        526     192      45     609     213     479     817     336

Cuts at:   3217    3873    7387    7617 
   Size:        656    3514     230

  Fragments arranged by size: 

                3514     817     656     609     526     479     336     230
                 213     192      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0     507     615     616     807    1088    1175    1182    1242 
   Size:        507     108       1     191     281      87       7      60

Cuts at:   1242    1416    1590    1841    1975    2057    3163    3267    4284 
   Size:        174     174     251     134      82    1106     104    1017

Cuts at:   4284    4635    5790    5889    6353    7052    7380    7617 
   Size:        351    1155      99     464     699     328     237

  Fragments arranged by size: 

                1155    1106    1017     699     507     464     351     328
                 281     251     237     191     174     174     134     108
                 104      99      87      82      60       7       1
 
EcoRI G'AATT_C

Cuts at:      0    1081    7358    7617 
   Size:       1081    6277     259

  Fragments arranged by size: 

                6277    1081     259
 
EcoRV GAT'ATC

Cuts at:      0     135    7617 
   Size:        135    7482
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     175    6430    7617 
   Size:        175    6255    1187

  Fragments arranged by size: 

                6255    1187     175
 
HaeII r_GCGC'y

Cuts at:      0    2616    2744    4074    4957    5801    5957    7617 
   Size:       2616     128    1330     883     844     156    1660

  Fragments arranged by size: 

                2616    1660    1330     883     844     156     128
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     426     458    1309    1341    1458    1490    1567    1582 
   Size:        426      32     851      32     117      32      77      15

Cuts at:   1582    1599    1612    1614    1644    2801    2833    3108    3140 
   Size:         17      13       2      30    1157      32     275      32

Cuts at:   3140    3375    3407    4624    4656    5200    5232    5721    5753 
   Size:        235      32    1217      32     544      32     489      32

Cuts at:   5753    7617 
   Size:       1864

  Fragments arranged by size: 

                1864    1217    1157     851     544     489     426     275
                 235     117      77      32      32      32      32      32
                  32      32      32      32      30      17      15      13
                   2
 
HincII GTy'rAC

Cuts at:      0    1678    3022    7617 
   Size:       1678    1344    4595

  Fragments arranged by size: 

                4595    1678    1344
 
HindIII A'AGCT_T

Cuts at:      0     301    7617 
   Size:        301    7316
 
KpnI G_GTAC'C

Cuts at:      0    5431    7617 
   Size:       5431    2186
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2290    4959    5097    6648    6709    7617 
   Size:       2290    2669     138    1551      61     908

  Fragments arranged by size: 

                2669    2290    1551     908     138      61
 
MscI TGG'CCA

Cuts at:      0     827    4267    4873    4891    6523    7617 
   Size:        827    3440     606      18    1632    1094

  Fragments arranged by size: 

                3440    1632    1094     827     606      18
 
MslI CAynn'nnrTG

Cuts at:      0      99     833    2479    2821    4300    4654    4913    5068 
   Size:         99     734    1646     342    1479     354     259     155

Cuts at:   5068    7108    7403    7617 
   Size:       2040     295     214

  Fragments arranged by size: 

                2040    1646    1479     734     354     342     295     259
                 214     155      99
 
MspA1I CmG'CkG

Cuts at:      0     782    3686    5052    5109    5510    5859    5943    6097 
   Size:        782    2904    1366      57     401     349      84     154

Cuts at:   6097    6294    6551    6973    7005    7197    7532    7617 
   Size:        197     257     422      32     192     335      85

  Fragments arranged by size: 

                2904    1366     782     422     401     349     335     257
                 197     192     154      85      84      57      32
 
NaeI GCC'GGC

Cuts at:      0    5518    5979    7617 
   Size:       5518     461    1638

  Fragments arranged by size: 

                5518    1638     461
 
NcoI C'CATG_G

Cuts at:      0    2912    7596    7617 
   Size:       2912    4684      21

  Fragments arranged by size: 

                4684    2912      21
 
NgoMIV G'CCGG_C

Cuts at:      0    5516    5977    7617 
   Size:       5516     461    1640

  Fragments arranged by size: 

                5516    1640     461
 
NheI G'CTAG_C

Cuts at:      0     921    7617 
   Size:        921    6696
 
NsiI A_TGCA'T

Cuts at:      0    4413    7617 
   Size:       4413    3204
 
NspI r_CATG'y

Cuts at:      0     104    4370    6202    7617 
   Size:        104    4266    1832    1415

  Fragments arranged by size: 

                4266    1832    1415     104
 
PasI CC'CwG_GG

Cuts at:      0     634    1179    1902    1972    7617 
   Size:        634     545     723      70    5645

  Fragments arranged by size: 

                5645     723     634     545      70
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1060    2637    4274    5994    6126    7239    7374    7617 
   Size:       1060    1577    1637    1720     132    1113     135     243

  Fragments arranged by size: 

                1720    1637    1577    1113    1060     243     135     132
            
 
PfoI T'CCnGG_A

Cuts at:      0    1244    2828    7609    7617 
   Size:       1244    1584    4781       8

  Fragments arranged by size: 

                4781    1584    1244       8
 
PmlI CAC'GTG

Cuts at:      0     402    6066    7617 
   Size:        402    5664    1551

  Fragments arranged by size: 

                5664    1551     402
 
PpuMI rG'GwC_Cy

Cuts at:      0    1175    1242    1416    1590    1841    3267    4635    7052 
   Size:       1175      67     174     174     251    1426    1368    2417

Cuts at:   7052    7617 
   Size:        565

  Fragments arranged by size: 

                2417    1426    1368    1175     565     251     174     174
                  67
 
PshAI GACnn'nnGTC

Cuts at:      0    2900    4930    7617 
   Size:       2900    2030    2687

  Fragments arranged by size: 

                2900    2687    2030
 
PspOMI G'GGCC_C

Cuts at:      0     615    1182    4283    7617 
   Size:        615     567    3101    3334

  Fragments arranged by size: 

                3334    3101     615     567
 
PspXI vC'TCGA_Gb

Cuts at:      0     358    1003    7617 
   Size:        358     645    6614

  Fragments arranged by size: 

                6614     645     358
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    6457    6489    7617 
   Size:       6457      32    1128

  Fragments arranged by size: 

                6457    1128      32
 
PstI C_TGCA'G

Cuts at:      0     569     742    2168    2748    3478    3492    4047    4556 
   Size:        569     173    1426     580     730      14     555     509

Cuts at:   4556    5262    5467    5569    5878    6102    6773    6972    7010 
   Size:        706     205     102     309     224     671     199      38

Cuts at:   7010    7187    7222    7617 
   Size:        177      35     395

  Fragments arranged by size: 

                1426     730     706     671     580     569     555     509
                 395     309     224     205     199     177     173     102
                  38      35      14
 
PvuII CAG'CTG

Cuts at:      0     782    3686    5052    5109    5859    5943    6097    6294 
   Size:        782    2904    1366      57     750      84     154     197

Cuts at:   6294    6551    6973    7005    7197    7617 
   Size:        257     422      32     192     420

  Fragments arranged by size: 

                2904    1366     782     750     422     420     257     197
                 192     154      84      57      32
 
SacI G_AGCT'C

Cuts at:      0     521    1202    3514    3574    3823    7617 
   Size:        521     681    2312      60     249    3794

  Fragments arranged by size: 

                3794    2312     681     521     249      60
 
SalI G'TCGA_C

Cuts at:      0    3020    7617 
   Size:       3020    4597
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1816    3579    6572    7617 
   Size:       1816    1763    2993    1045

  Fragments arranged by size: 

                2993    1816    1763    1045
 
SbfI CC_TGCA'GG

Cuts at:      0     742    7617 
   Size:        742    6875
 
ScaI AGT'ACT

Cuts at:      0    6139    6709    7617 
   Size:       6139     570     908

  Fragments arranged by size: 

                6139     908     570
 
SexAI A'CCwGG_T

Cuts at:      0    7021    7617 
   Size:       7021     596
 
SfcI C'TryA_G

Cuts at:      0     115     565     738     869    1604    2164    2725    2744 
   Size:        115     450     173     131     735     560     561      19

Cuts at:   2744    3474    3488    4043    4435    4531    4552    5258    5463 
   Size:        730      14     555     392      96      21     706     205

Cuts at:   5463    5565    5601    5874    6098    6507    6769    6968    7006 
   Size:        102      36     273     224     409     262     199      38

Cuts at:   7006    7183    7218    7617 
   Size:        177      35     399

  Fragments arranged by size: 

                 735     730     706     561     560     555     450     409
                 399     392     273     262     224     205     199     177
                 173     131     115     102      96      38      36      35
                  21      19      14
 
SmaI CCC'GGG

Cuts at:      0     265    4060    4282    7588    7617 
   Size:        265    3795     222    3306      29

  Fragments arranged by size: 

                3795    3306     265     222      29
 
SmlI C'TyrA_G

Cuts at:      0     304     358     526    1003    1387    3922    4614    6306 
   Size:        304      54     168     477     384    2535     692    1692

Cuts at:   6306    6877    7617 
   Size:        571     740

  Fragments arranged by size: 

                2535    1692     740     692     571     477     384     304
                 168      54
 
SpeI A'CTAG_T

Cuts at:      0    2014    7617 
   Size:       2014    5603
 
SphI G_CATG'C

Cuts at:      0    6202    7617 
   Size:       6202    1415
 
SspI AAT'ATT

Cuts at:      0    6039    7617 
   Size:       6039    1578
 
StuI AGG'CCT

Cuts at:      0     736    4102    7617 
   Size:        736    3366    3515

  Fragments arranged by size: 

                3515    3366     736
 
StyI C'CwwG_G

Cuts at:      0     333     418    1412    2189    2912    3874    3911    4259 
   Size:        333      85     994     777     723     962      37     348

Cuts at:   4259    5885    6689    6884    7596    7617 
   Size:       1626     804     195     712      21

  Fragments arranged by size: 

                1626     994     962     804     777     723     712     348
                 333     195      85      37      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     760    1784    2598    4921    7617 
   Size:        760    1024     814    2323    2696

  Fragments arranged by size: 

                2696    2323    1024     814     760
 
TatI w'GTAC_w

Cuts at:      0     243    1107    2368    4233    6137    6707    7253    7617 
   Size:        243     864    1261    1865    1904     570     546     364

  Fragments arranged by size: 

                1904    1865    1261     864     570     546     364     243
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1537    1569    4611    4643    7617 
   Size:       1537      32    3042      32    2974

  Fragments arranged by size: 

                3042    2974    1537      32      32
 
XbaI T'CTAG_A

Cuts at:      0     900    4662    4764    5655    7617 
   Size:        900    3762     102     891    1962

  Fragments arranged by size: 

                3762    1962     900     891     102
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     953    5547    5904    7617 
   Size:        953    4594     357    1713

  Fragments arranged by size: 

                4594    1713     953     357
 
XhoI C'TCGA_G

Cuts at:      0     358    1003    7617 
   Size:        358     645    6614

  Fragments arranged by size: 

                6614     645     358
 
XmaI C'CCGG_G

Cuts at:      0     263    4058    4280    7586    7617 
   Size:        263    3795     222    3306      31

  Fragments arranged by size: 

                3795    3306     263     222      31
 
XmnI GAAnn'nnTTC

Cuts at:      0    1382    3191    6566    7617 
   Size:       1382    1809    3375    1051

  Fragments arranged by size: 

                3375    1809    1382    1051
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NheI     NsiI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SmaI     SmlI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AgeI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BsiEI    BsiWI    BsrGI   BssHII    BtgZI     ClaI
     DraI     EagI     FseI     FspI    FspAI     HpaI     KasI     MfeI
     MluI     NarI     NdeI     NotI     NruI     PacI     PciI     PmeI
     PpiI     PsiI     PvuI    RsrII    SacII    SanDI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SrfI     SwaI  Tth111I     ZraI
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