Restriction map for pF1KA0305
 (Linear) (Six-base) MAPSORT of: KIAA0305.seq  Check: 9341  from: 1  to: 4617



KIAA0305.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AccI GT'mk_AC

Cuts at:      0    2526    3709    3882    4127    4617 
   Size:       2526    1183     173     245     490

  Fragments arranged by size: 

                2526    1183     490     245     173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     314     485    1067    1315    1609    2156    2708 
   Size:        116     198     171     582     248     294     547     552

Cuts at:   2708    2945    3327    3974    4187    4502    4617 
   Size:        237     382     647     213     315     115

  Fragments arranged by size: 

                 647     582     552     547     382     315     294     248
                 237     213     198     171     116     115
 
AflII C'TTAA_G

Cuts at:      0    1051    2461    4617 
   Size:       1051    1410    2156

  Fragments arranged by size: 

                2156    1410    1051
 
AflIII A'CryG_T

Cuts at:      0     728    1092    4105    4617 
   Size:        728     364    3013     512

  Fragments arranged by size: 

                3013     728     512     364
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2503    2535    4617 
   Size:       2503      32    2082

  Fragments arranged by size: 

                2503    2082      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1909    2923    3106    4480    4617 
   Size:       1909    1014     183    1374     137

  Fragments arranged by size: 

                1909    1374    1014     183     137
 
ApoI r'AATT_y

Cuts at:      0     675     951     966    2275    3214    3524    3810    3896 
   Size:        675     276      15    1309     939     310     286      86

Cuts at:   3896    4465    4617 
   Size:        569     152

  Fragments arranged by size: 

                1309     939     675     569     310     286     276     152
                  86      15
 
AseI AT'TA_AT

Cuts at:      0    1162    1339    1499    1775    4617 
   Size:       1162     177     160     276    2842

  Fragments arranged by size: 

                2842    1162     276     177     160
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2036    2069    4617 
   Size:       2036      33    2548

  Fragments arranged by size: 

                2548    2036      33
 
BanII G_rGCy'C

Cuts at:      0    3978    4617 
   Size:       3978     639
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1037    1123    1361    1466    2696    3031    4617 
   Size:       1037      86     238     105    1230     335    1586

  Fragments arranged by size: 

                1586    1230    1037     335     238     105      86
 
BbvCI CC'TCA_GC

Cuts at:      0    1196    4499    4617 
   Size:       1196    3303     118

  Fragments arranged by size: 

                3303    1196     118
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2285    2319    4617 
   Size:       2285      34    2298

  Fragments arranged by size: 

                2298    2285      34
 
BfrBI ATG'CAT

Cuts at:      0     103    4617 
   Size:        103    4514
 
BglII A'GATC_T

Cuts at:      0     692    4617 
   Size:        692    3925
 
Bme1580I G_kGCm'C

Cuts at:      0    4390    4617 
   Size:       4390     227
 
BmtI G_CTAG'C

Cuts at:      0    4075    4617 
   Size:       4075     542
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3107    4031    4617 
   Size:       3107     924     586

  Fragments arranged by size: 

                3107     924     586
 
Bpu10I CC'TnA_GC

Cuts at:      0    1196    1946    2420    4499    4617 
   Size:       1196     750     474    2079     118

  Fragments arranged by size: 

                2079    1196     750     474     118
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238    1424    3562    4617 
   Size:        238    1186    2138    1055

  Fragments arranged by size: 

                2138    1186    1055     238
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4045    4617 
   Size:       4045     572
 
BsaAI yAC'GTr

Cuts at:      0    4143    4617 
   Size:       4143     474
 
BsaBI GATnn'nnATC

Cuts at:      0    3869    4617 
   Size:       3869     748
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2492    3608    4617 
   Size:       2492    1116    1009

  Fragments arranged by size: 

                2492    1116    1009
 
BseYI C'CCAG_C

Cuts at:      0    2032    4154    4617 
   Size:       2032    2122     463

  Fragments arranged by size: 

                2122    2032     463
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    3614    4617 
   Size:       1007    2607    1003

  Fragments arranged by size: 

                2607    1007    1003
 
BsiHKAI G_wGCw'C

Cuts at:      0    3978    4530    4617 
   Size:       3978     552      87

  Fragments arranged by size: 

                3978     552      87
 
Bsp1286I G_dGCh'C

Cuts at:      0    3978    4390    4530    4617 
   Size:       3978     412     140      87

  Fragments arranged by size: 

                3978     412     140      87
 
BspHI T'CATG_A

Cuts at:      0    3129    4617 
   Size:       3129    1488
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     916    4617 
   Size:        916    3701
 
BsrDI GCAATG_nn'

Cuts at:      0     160    1521    2301    4617 
   Size:        160    1361     780    2316

  Fragments arranged by size: 

                2316    1361     780     160
 
BsrGI T'GTAC_A

Cuts at:      0      93    4617 
   Size:         93    4524
 
BssSI C'ACGA_G

Cuts at:      0    3202    4617 
   Size:       3202    1415
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3613    4617 
   Size:       3613    1004
 
BstBI TT'CG_AA

Cuts at:      0    3696    4617 
   Size:       3696     921
 
BstEII G'GTnAC_C

Cuts at:      0    1865    2873    4617 
   Size:       1865    1008    1744

  Fragments arranged by size: 

                1865    1744    1008
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     805    4617 
   Size:        805    3812
 
BstYI r'GATC_y

Cuts at:      0     692    1368    2143    4477    4617 
   Size:        692     676     775    2334     140

  Fragments arranged by size: 

                2334     775     692     676     140
 
BstZ17I GTA'TAC

Cuts at:      0    2527    3710    3883    4617 
   Size:       2527    1183     173     734

  Fragments arranged by size: 

                2527    1183     734     173
 
Bsu36I CC'TnA_GG

Cuts at:      0    2504    3332    4617 
   Size:       2504     828    1285

  Fragments arranged by size: 

                2504    1285     828
 
BtgI C'CryG_G

Cuts at:      0    3775    4364    4545    4617 
   Size:       3775     589     181      72

  Fragments arranged by size: 

                3775     589     181      72
 
BtsI GCAGTG_nn'

Cuts at:      0     117    1235    4617 
   Size:        117    1118    3382

  Fragments arranged by size: 

                3382    1118     117
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3941    3976    4617 
   Size:       3941      35     641

  Fragments arranged by size: 

                3941     641      35
 
DraI TTT'AAA

Cuts at:      0      15     680     878    4098    4617 
   Size:         15     665     198    3220     519

  Fragments arranged by size: 

                3220     665     519     198      15
 
DraIII CAC_nnn'GTG

Cuts at:      0    1183    4617 
   Size:       1183    3434
 
EaeI y'GGCC_r

Cuts at:      0    3927    4617 
   Size:       3927     690
 
EarI CTCTTCn'nnn_

Cuts at:      0     878    1086    2596    4617 
   Size:        878     208    1510    2021

  Fragments arranged by size: 

                2021    1510     878     208
 
EcoICRI GAG'CTC

Cuts at:      0    3976    4617 
   Size:       3976     641
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     314     485    1067    1315    1609    2156    2708 
   Size:        116     198     171     582     248     294     547     552

Cuts at:   2708    2945    3107    3327    3974    4031    4187    4502    4617 
   Size:        237     162     220     647      57     156     315     115

  Fragments arranged by size: 

                 647     582     552     547     315     294     248     237
                 220     198     171     162     156     116     115      57
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1989    4553    4617 
   Size:       1989    2564      64

  Fragments arranged by size: 

                2564    1989      64
 
EcoRI G'AATT_C

Cuts at:      0    3524    4617 
   Size:       3524    1093
 
EcoRV GAT'ATC

Cuts at:      0    4149    4617 
   Size:       4149     468
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1465    3357    3527    4507    4617 
   Size:       1465    1892     170     980     110

  Fragments arranged by size: 

                1892    1465     980     170     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     684     716    1099    1131    1657    1689 
   Size:        448      32     204      32     383      32     526      32

Cuts at:   1689    1878    1910    2427    2459    3338    3370    3478    3510 
   Size:        189      32     517      32     879      32     108      32

Cuts at:   3510    4195    4227    4617 
   Size:        685      32     390

  Fragments arranged by size: 

                 879     685     526     517     448     390     383     204
                 189     108      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2958    3690    4617 
   Size:       2958     732     927

  Fragments arranged by size: 

                2958     927     732
 
HindIII A'AGCT_T

Cuts at:      0    3538    4617 
   Size:       3538    1079
 
HpaI GTT'AAC

Cuts at:      0    2958    4617 
   Size:       2958    1659
 
MfeI C'AATT_G

Cuts at:      0     713    1951    4617 
   Size:        713    1238    2666

  Fragments arranged by size: 

                2666    1238     713
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1911    2470    4583    4617 
   Size:       1911     559    2113      34

  Fragments arranged by size: 

                2113    1911     559      34
 
MscI TGG'CCA

Cuts at:      0    3929    4617 
   Size:       3929     688
 
MslI CAynn'nnrTG

Cuts at:      0     542    2477    3673    4456    4617 
   Size:        542    1935    1196     783     161

  Fragments arranged by size: 

                1935    1196     783     542     161
 
MspA1I CmG'CkG

Cuts at:      0      22    2083    4617 
   Size:         22    2061    2534

  Fragments arranged by size: 

                2534    2061      22
 
NcoI C'CATG_G

Cuts at:      0    4364    4545    4617 
   Size:       4364     181      72

  Fragments arranged by size: 

                4364     181      72
 
NdeI CA'TA_TG

Cuts at:      0     105    3743    4617 
   Size:        105    3638     874

  Fragments arranged by size: 

                3638     874     105
 
NheI G'CTAG_C

Cuts at:      0    4071    4617 
   Size:       4071     546
 
NsiI A_TGCA'T

Cuts at:      0     105    4617 
   Size:        105    4512
 
NspI r_CATG'y

Cuts at:      0     732    1096    2315    3829    4109    4617 
   Size:        732     364    1219    1514     280     508

  Fragments arranged by size: 

                1514    1219     732     508     364     280
 
PasI CC'CwG_GG

Cuts at:      0    3551    4617 
   Size:       3551    1066
 
PciI A'CATG_T

Cuts at:      0     728    1092    4105    4617 
   Size:        728     364    3013     512

  Fragments arranged by size: 

                3013     728     512     364
 
PpuMI rG'GwC_Cy

Cuts at:      0    1989    4553    4617 
   Size:       1989    2564      64

  Fragments arranged by size: 

                2564    1989      64
 
PshAI GACnn'nnGTC

Cuts at:      0    2696    4617 
   Size:       2696    1921
 
PsiI TTA'TAA

Cuts at:      0    1012    4617 
   Size:       1012    3605
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2667    2699    4617 
   Size:       2667      32    1918

  Fragments arranged by size: 

                2667    1918      32
 
PstI C_TGCA'G

Cuts at:      0     913    4617 
   Size:        913    3704
 
PvuII CAG'CTG

Cuts at:      0      22    2083    4617 
   Size:         22    2061    2534

  Fragments arranged by size: 

                2534    2061      22
 
SacI G_AGCT'C

Cuts at:      0    3978    4617 
   Size:       3978     639
 
ScaI AGT'ACT

Cuts at:      0    3806    4617 
   Size:       3806     811
 
SexAI A'CCwGG_T

Cuts at:      0    2568    4617 
   Size:       2568    2049
 
SfcI C'TryA_G

Cuts at:      0     909    1424    2069    2078    2756    3107    4617 
   Size:        909     515     645       9     678     351    1510

  Fragments arranged by size: 

                1510     909     678     645     515     351       9
 
SmlI C'TyrA_G

Cuts at:      0     253    1051    1439    2461    3541    4617 
   Size:        253     798     388    1022    1080    1076

  Fragments arranged by size: 

                1080    1076    1022     798     388     253
 
SspI AAT'ATT

Cuts at:      0    1551    3241    3408    4617 
   Size:       1551    1690     167    1209

  Fragments arranged by size: 

                1690    1551    1209     167
 
StyI C'CwwG_G

Cuts at:      0    4364    4545    4617 
   Size:       4364     181      72

  Fragments arranged by size: 

                4364     181      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3580    4617 
   Size:       3580    1037
 
TatI w'GTAC_w

Cuts at:      0      93    2485    3785    3804    3853    4617 
   Size:         93    2392    1300      19      49     764

  Fragments arranged by size: 

                2392    1300     764      93      49      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3915    3947    4617 
   Size:       3915      32     670

  Fragments arranged by size: 

                3915     670      32
 
XbaI T'CTAG_A

Cuts at:      0    2367    4617 
   Size:       2367    2250
 
XmnI GAAnn'nnTTC

Cuts at:      0    3164    4617 
   Size:       3164    1453
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AloI    AlwNI     ApoI     AseI
     BaeI    BanII     BbsI    BbvCI     BcgI    BfrBI    BglII Bme1580I
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI    CspCI     DraI   DraIII     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII
     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NsiI     NspI     PasI     PciI    PpuMI    PshAI     PsiI
     PsrI     PstI    PvuII     SacI     ScaI    SexAI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI
     BanI     BbeI    BciVI     BclI     BglI     BlpI    BmgBI     BmrI
     BplI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BtgZI     ClaI     DrdI     EagI
     EciI    EcoNI     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MluI     NaeI     NarI   NgoMIV     NotI     NruI     PacI    PflMI
     PfoI     PmeI     PmlI     PpiI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XcmI     XhoI     XmaI     ZraI
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