Restriction map for pF1KA0244
 (Linear) (Six-base) MAPSORT of: KIAA0244.seq  Check: 6904  from: 1  to: 6117



KIAA0244.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AccI GT'mk_AC

Cuts at:      0    2638    3595    5975    6117 
   Size:       2638     957    2380     142

  Fragments arranged by size: 

                2638    2380     957     142
 
Acc65I G'GTAC_C

Cuts at:      0     627    2847    6071    6117 
   Size:        627    2220    3224      46

  Fragments arranged by size: 

                3224    2220     627      46
 
AclI AA'CG_TT

Cuts at:      0    3544    6117 
   Size:       3544    2573
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     644     653    1384    2333    3854    3858    4072 
   Size:        154     490       9     731     949    1521       4     214

Cuts at:   4072    4234    4774    4976    5398    6117 
   Size:        162     540     202     422     719

  Fragments arranged by size: 

                1521     949     731     719     540     490     422     214
                 202     162     154       9       4
 
AfeI AGC'GCT

Cuts at:      0    3889    6117 
   Size:       3889    2228
 
AflII C'TTAA_G

Cuts at:      0     339    6117 
   Size:        339    5778
 
AflIII A'CryG_T

Cuts at:      0    1851    5308    6117 
   Size:       1851    3457     809

  Fragments arranged by size: 

                3457    1851     809
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3300    6117 
   Size:       3300    2817
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3685    3832    4050    4070    5724    6117 
   Size:       3685     147     218      20    1654     393

  Fragments arranged by size: 

                3685    1654     393     218     147      20
 
ApaLI G'TGCA_C

Cuts at:      0    4109    5251    6117 
   Size:       4109    1142     866

  Fragments arranged by size: 

                4109    1142     866
 
ApoI r'AATT_y

Cuts at:      0     360    1338    1617    1760    2374    2483    2514    2838 
   Size:        360     978     279     143     614     109      31     324

Cuts at:   2838    3505    3658    3803    3845    3962    4484    4722    5320 
   Size:        667     153     145      42     117     522     238     598

Cuts at:   5320    5646    6117 
   Size:        326     471

  Fragments arranged by size: 

                 978     667     614     598     522     471     360     326
                 324     279     238     153     145     143     117     109
                  42      31
 
AvrII C'CTAG_G

Cuts at:      0    4954    6117 
   Size:       4954    1163
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     559     592    1583    1616    6117 
   Size:        559      33     991      33    4501

  Fragments arranged by size: 

                4501     991     559      33      33
 
BamHI G'GATC_C

Cuts at:      0      67     156    4950    6117 
   Size:         67      89    4794    1167

  Fragments arranged by size: 

                4794    1167      89      67
 
BanI G'GyrC_C

Cuts at:      0     627    2847    3375    3949    4423    5684    6071    6117 
   Size:        627    2220     528     574     474    1261     387      46

  Fragments arranged by size: 

                2220    1261     627     574     528     474     387      46
            
 
BbeI G_GCGC'C

Cuts at:      0    5688    6117 
   Size:       5688     429
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     496     638    2285    2324    4262    6011    6117 
   Size:        496     142    1647      39    1938    1749     106

  Fragments arranged by size: 

                1938    1749    1647     496     142     106      39
 
BbvCI CC'TCA_GC

Cuts at:      0    1889    4238    6117 
   Size:       1889    2349    1879

  Fragments arranged by size: 

                2349    1889    1879
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5242    5276    6117 
   Size:       5242      34     841

  Fragments arranged by size: 

                5242     841      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1128    6117 
   Size:       1128    4989
 
BclI T'GATC_A

Cuts at:      0    2106    3021    6117 
   Size:       2106     915    3096

  Fragments arranged by size: 

                3096    2106     915
 
BfrBI ATG'CAT

Cuts at:      0    3472    6117 
   Size:       3472    2645
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4421    5682    6117 
   Size:       4421    1261     435

  Fragments arranged by size: 

                4421    1261     435
 
BglII A'GATC_T

Cuts at:      0     370    4849    6117 
   Size:        370    4479    1268

  Fragments arranged by size: 

                4479    1268     370
 
BlpI GC'TnA_GC

Cuts at:      0     145    6117 
   Size:        145    5972
 
Bme1580I G_kGCm'C

Cuts at:      0    4113    4426    5255    5897    5916    6117 
   Size:       4113     313     829     642      19     201

  Fragments arranged by size: 

                4113     829     642     313     201      19
 
BmgBI CAC'GTC

Cuts at:      0    3562    6117 
   Size:       3562    2555
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4446    6117 
   Size:       4446    1671
 
BmtI G_CTAG'C

Cuts at:      0    2560    6117 
   Size:       2560    3557
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2916    3312    5136    6117 
   Size:       2916     396    1824     981

  Fragments arranged by size: 

                2916    1824     981     396
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97    5510    6117 
   Size:         97    5413     607

  Fragments arranged by size: 

                5413     607      97
 
Bpu10I CC'TnA_GC

Cuts at:      0    1889    4238    6117 
   Size:       1889    2349    1879

  Fragments arranged by size: 

                2349    1889    1879
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    2240    5177    5407    6117 
   Size:       1027    1213    2937     230     710

  Fragments arranged by size: 

                2937    1213    1027     710     230
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4928    6117 
   Size:       4928    1189
 
BsaAI yAC'GTr

Cuts at:      0    1926    6117 
   Size:       1926    4191
 
BsaBI GATnn'nnATC

Cuts at:      0     155    6117 
   Size:        155    5962
 
BsaHI Gr'CG_yC

Cuts at:      0    5685    6117 
   Size:       5685     432
 
BsaWI w'CCGG_w

Cuts at:      0    1290    6117 
   Size:       1290    4827
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1497    1527    3534    3564    6117 
   Size:       1497      30    2007      30    2553

  Fragments arranged by size: 

                2553    2007    1497      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2106    2552    3301    3529    3532    6117 
   Size:       2106     446     749     228       3    2585

  Fragments arranged by size: 

                2585    2106     749     446     228       3
 
BseYI C'CCAG_C

Cuts at:      0    1902    2146    4346    6117 
   Size:       1902     244    2200    1771

  Fragments arranged by size: 

                2200    1902    1771     244
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618    1823    6117 
   Size:        618    1205    4294

  Fragments arranged by size: 

                4294    1205     618
 
BsiHKAI G_wGCw'C

Cuts at:      0     433    1240    4113    5069    5255    6117 
   Size:        433     807    2873     956     186     862

  Fragments arranged by size: 

                2873     956     862     807     433     186
 
BsmI GAATG_Cn'

Cuts at:      0    1359    6117 
   Size:       1359    4758
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3072    6117 
   Size:       3072    3045
 
Bsp1286I G_dGCh'C

Cuts at:      0     433    1240    4113    4426    5069    5255    5897    5916 
   Size:        433     807    2873     313     643     186     642      19

Cuts at:   5916    6117 
   Size:        201

  Fragments arranged by size: 

                2873     807     643     642     433     313     201     186
                  19
 
BspHI T'CATG_A

Cuts at:      0     750    2685    6117 
   Size:        750    1935    3432

  Fragments arranged by size: 

                3432    1935     750
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3909    6117 
   Size:       3909    2208
 
BsrDI GCAATG_nn'

Cuts at:      0     216     300    1913    4123    6117 
   Size:        216      84    1613    2210    1994

  Fragments arranged by size: 

                2210    1994    1613     216      84
 
BsrGI T'GTAC_A

Cuts at:      0    1048    2736    6117 
   Size:       1048    1688    3381

  Fragments arranged by size: 

                3381    1688    1048
 
BssSI C'ACGA_G

Cuts at:      0    5121    6117 
   Size:       5121     996
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2872    3359    6117 
   Size:       2872     487    2758

  Fragments arranged by size: 

                2872    2758     487
 
BstBI TT'CG_AA

Cuts at:      0    5262    6117 
   Size:       5262     855
 
BstYI r'GATC_y

Cuts at:      0      67     156     169     370    1018    2340    2599    2967 
   Size:         67      89      13     201     648    1322     259     368

Cuts at:   2967    3268    4849    4950    6117 
   Size:        301    1581     101    1167

  Fragments arranged by size: 

                1581    1322    1167     648     368     301     259     201
                 101      89      67      13
 
BtgI C'CryG_G

Cuts at:      0    1139    3218    5552    6117 
   Size:       1139    2079    2334     565

  Fragments arranged by size: 

                2334    2079    1139     565
 
BtsI GCAGTG_nn'

Cuts at:      0    2161    6117 
   Size:       2161    3956
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2139    2174    2865    2900    5483    5518    6117 
   Size:       2139      35     691      35    2583      35     599

  Fragments arranged by size: 

                2583    2139     691     599      35      35      35
 
DraI TTT'AAA

Cuts at:      0    2489    2991    4496    5147    6117 
   Size:       2489     502    1505     651     970

  Fragments arranged by size: 

                2489    1505     970     651     502
 
DraIII CAC_nnn'GTG

Cuts at:      0    5929    6117 
   Size:       5929     188
 
EaeI y'GGCC_r

Cuts at:      0    3142    5563    6117 
   Size:       3142    2421     554

  Fragments arranged by size: 

                3142    2421     554
 
EarI CTCTTCn'nnn_

Cuts at:      0    1928    1964    2088    2570    2815    3078    4394    4647 
   Size:       1928      36     124     482     245     263    1316     253

Cuts at:   4647    5259    6117 
   Size:        612     858

  Fragments arranged by size: 

                1928    1316     858     612     482     263     253     245
                 124      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     154     644     653    1384    2333    3854    3858 
   Size:         97      57     490       9     731     949    1521       4

Cuts at:   3858    4072    4234    4774    4976    5398    5510    6117 
   Size:        214     162     540     202     422     112     607

  Fragments arranged by size: 

                1521     949     731     607     540     490     422     214
                 202     162     112      97      57       9       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     164    3706    6117 
   Size:        164    3542    2411

  Fragments arranged by size: 

                3542    2411     164
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1899    3711    4637    5455    5608    6117 
   Size:       1899    1812     926     818     153     509

  Fragments arranged by size: 

                1899    1812     926     818     509     153
 
EcoRI G'AATT_C

Cuts at:      0    2374    3505    6117 
   Size:       2374    1131    2612

  Fragments arranged by size: 

                2612    2374    1131
 
EcoRV GAT'ATC

Cuts at:      0    3746    6117 
   Size:       3746    2371
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     674    2060    2746    2830    5085    6117 
   Size:        674    1386     686      84    2255    1032

  Fragments arranged by size: 

                2255    1386    1032     686     674      84
 
HaeII r_GCGC'y

Cuts at:      0    3891    5688    6117 
   Size:       3891    1797     429

  Fragments arranged by size: 

                3891    1797     429
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     730     762    1003    1035    1201    1233    2098    2130 
   Size:        730      32     241      32     166      32     865      32

Cuts at:   2130    2786    2818    3131    3163    3286    3318    3460    3492 
   Size:        656      32     313      32     123      32     142      32

Cuts at:   3492    3735    3767    4659    4691    5595    5627    5809    5841 
   Size:        243      32     892      32     904      32     182      32

Cuts at:   5841    6117 
   Size:        276

  Fragments arranged by size: 

                 904     892     865     730     656     313     276     243
                 241     182     166     142     123      32      32      32
                  32      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3294    3322    3542    6117 
   Size:       3294      28     220    2575

  Fragments arranged by size: 

                3294    2575     220      28
 
HindIII A'AGCT_T

Cuts at:      0     812    2410    2666    6117 
   Size:        812    1598     256    3451

  Fragments arranged by size: 

                3451    1598     812     256
 
KasI G'GCGC_C

Cuts at:      0    5684    6117 
   Size:       5684     433
 
KpnI G_GTAC'C

Cuts at:      0     631    2851    6075    6117 
   Size:        631    2220    3224      42

  Fragments arranged by size: 

                3224    2220     631      42
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95    1211    4288    5280    6117 
   Size:         95    1116    3077     992     837

  Fragments arranged by size: 

                3077    1116     992     837      95
 
MscI TGG'CCA

Cuts at:      0    5565    6117 
   Size:       5565     552
 
MslI CAynn'nnrTG

Cuts at:      0      47     992    2815    3106    3167    6117 
   Size:         47     945    1823     291      61    2950

  Fragments arranged by size: 

                2950    1823     945     291      61      47
 
NarI GG'CG_CC

Cuts at:      0    5685    6117 
   Size:       5685     432
 
NcoI C'CATG_G

Cuts at:      0    1139    3218    5552    6117 
   Size:       1139    2079    2334     565

  Fragments arranged by size: 

                2334    2079    1139     565
 
NheI G'CTAG_C

Cuts at:      0    2556    6117 
   Size:       2556    3561
 
NsiI A_TGCA'T

Cuts at:      0    3474    6117 
   Size:       3474    2643
 
NspI r_CATG'y

Cuts at:      0     144    1855    3645    5040    5312    5484    6117 
   Size:        144    1711    1790    1395     272     172     633

  Fragments arranged by size: 

                1790    1711    1395     633     272     172     144
 
PciI A'CATG_T

Cuts at:      0    1851    5308    6117 
   Size:       1851    3457     809

  Fragments arranged by size: 

                3457    1851     809
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3685    5533    6117 
   Size:       3685    1848     584

  Fragments arranged by size: 

                3685    1848     584
 
PfoI T'CCnGG_A

Cuts at:      0     726    6117 
   Size:        726    5391
 
PmlI CAC'GTG

Cuts at:      0    1926    6117 
   Size:       1926    4191
 
PpuMI rG'GwC_Cy

Cuts at:      0    3711    4637    5608    6117 
   Size:       3711     926     971     509

  Fragments arranged by size: 

                3711     971     926     509
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     308     340    6117 
   Size:        308      32    5777

  Fragments arranged by size: 

                5777     308      32
 
PstI C_TGCA'G

Cuts at:      0    2772    4656    5112    6117 
   Size:       2772    1884     456    1005

  Fragments arranged by size: 

                2772    1884    1005     456
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1928    6117 
   Size:       1928    4189
 
ScaI AGT'ACT

Cuts at:      0    1281    1368    2999    4080    6117 
   Size:       1281      87    1631    1081    2037

  Fragments arranged by size: 

                2037    1631    1281    1081      87
 
SexAI A'CCwGG_T

Cuts at:      0    2676    5654    6117 
   Size:       2676    2978     463

  Fragments arranged by size: 

                2978    2676     463
 
SfcI C'TryA_G

Cuts at:      0    1358    2366    2526    2768    3383    4160    4598    4652 
   Size:       1358    1008     160     242     615     777     438      54

Cuts at:   4652    4703    4890    5108    6117 
   Size:         51     187     218    1009

  Fragments arranged by size: 

                1358    1009    1008     777     615     438     242     218
                 187     160      54      51
 
SfoI GGC'GCC

Cuts at:      0    5686    6117 
   Size:       5686     431
 
SmlI C'TyrA_G

Cuts at:      0     339    1006    2255    5192    5422    6117 
   Size:        339     667    1249    2937     230     695

  Fragments arranged by size: 

                2937    1249     695     667     339     230
 
SpeI A'CTAG_T

Cuts at:      0    1276    3316    4081    6117 
   Size:       1276    2040     765    2036

  Fragments arranged by size: 

                2040    2036    1276     765
 
SspI AAT'ATT

Cuts at:      0    3492    4587    5241    6117 
   Size:       3492    1095     654     876

  Fragments arranged by size: 

                3492    1095     876     654
 
StyI C'CwwG_G

Cuts at:      0    1139    3218    3797    4954    5552    5698    6117 
   Size:       1139    2079     579    1157     598     146     419

  Fragments arranged by size: 

                2079    1157    1139     598     579     419     146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1961    3937    5270    5403    6117 
   Size:       1961    1976    1333     133     714

  Fragments arranged by size: 

                1976    1961    1333     714     133
 
TatI w'GTAC_w

Cuts at:      0    1048    1279    1366    1722    1755    2736    2997    4078 
   Size:       1048     231      87     356      33     981     261    1081

Cuts at:   4078    5374    6117 
   Size:       1296     743

  Fragments arranged by size: 

                1296    1081    1048     981     743     356     261     231
                  87      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1497    1529    5086    5118    6117 
   Size:       1497      32    3557      32     999

  Fragments arranged by size: 

                3557    1497     999      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4973    6117 
   Size:       4973    1144
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3249    6117 
   Size:       3249    2868
 
XmnI GAAnn'nnTTC

Cuts at:      0    4259    6117 
   Size:       4259    1858
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AhdI
    AlwNI    ApaLI     ApoI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI     BtsI
    CspCI     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MmeI     MscI     MslI     NarI     NcoI     NheI     NsiI
     NspI     PciI    PflMI     PfoI     PmlI    PpuMI     PsrI     PstI
     SapI     ScaI    SexAI     SfcI     SfoI     SmlI     SpeI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AgeI     AleI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI     AvaI    BanII    BsiEI    BsiWI    BspEI    BsrBI
    BsrFI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DrdI     EagI     EciI  EcoICRI     FseI     FspI    FspAI     HpaI
     MfeI     MluI   MspA1I     NaeI     NdeI   NgoMIV     NotI     NruI
     PacI     PasI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI
     SwaI     XbaI     XhoI     XmaI     ZraI
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