Restriction map for pF1KA0130
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BfrI        
BlnI        BsaAI       BsaBI       BsiEI       BsiWI       
BspDI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstZ17I     ClaI        DraI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
FseI        FspI        HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII        305  ACATGT               A^C[GA][CT]GT
AocI         1600  CCTGAGG              CC^T[ACGT]AGG
AspI          777  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI         2512  GAAGAC               GAAGAC(2/6)
BcgI         2564  CGAAGTTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1991  TGATCA               T^GATCA
BglI         2428  GCCAAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         851  AGATCT               A^GATCT
BpuAI        2512  GAAGAC               GAAGAC(2/6)
Bsp1407I     1075  TGTACA               T^GTACA
BspHI        1994  TCATGA               T^CATGA
BspLU11I      305  ACATGT               A^CATGT
BsrGI        1075  TGTACA               T^GTACA
BstBI        2562  TTCGAA               TT^CGAA
Bsu36I       1600  CCTGAGG              CC^T[ACGT]AGG
CpoI         1160  CGGACCG              CG^G[AT]CCG
DraIII        733  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          271  GACCTGTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2817  CCGTGG               C^C[GA][CT]GG
Eco81I       1600  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1417  GAATTC               G^AATTC
EcoT22I       348  ATGCAT               ATGCA^T
FbaI         1991  TGATCA               T^GATCA
HindIII       860  AAGCTT               A^AGCTT
MseI         2246  TTAA                 T^TAA
NaeI          442  GCCGGC               GCC^GGC
NgoMIV        442  GCCGGC               G^CCGGC
NsiI          348  ATGCAT               ATGCA^T
NspV         2562  TTCGAA               TT^CGAA
PaeR7I        748  CTCGAG               C^TCGAG
PflFI         777  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         451  CCAGCCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1994  TCATGA               T^CATGA
RsrII        1160  CGGACCG              CG^G[AT]CCG
ScaI          965  AGTACT               AGT^ACT
SfuI         2562  TTCGAA               TT^CGAA
SmaI         2412  CCCGGG               CCC^GGG
SspBI        1075  TGTACA               T^GTACA
Tru9I        2246  TTAA                 T^TAA
Tth111I       777  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        451  CCAGCCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          748  CTCGAG               C^TCGAG
XmaCI        2412  CCCGGG               C^CCGGG
XmaI         2412  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         2170  GTAGAC               GT^[AC][GT]AC
             2221  GTCTAC              
AccIII        632  TCCGGA               T^CCGGA
             1883  TCCGGA              
AcyI         2218  GGCGTC               G[GA]^CG[CT]C
             2481  GGCGTC              
Aor51HI        47  AGCGCT               AGC^GCT
             1058  AGCGCT              
ApaI          524  GGGCCC               GGGCC^C
             1466  GGGCCC              
BalI         2702  TGGCCA               TGG^CCA
             2903  TGGCCA              
BlpI         1147  GCTAAGC              GC^T[ACGT]AGC
             2580  GCTGAGC             
Bpu1102I     1147  GCTAAGC              GC^T[ACGT]AGC
             2580  GCTGAGC             
BsaHI        2218  GGCGTC               G[GA]^CG[CT]C
             2481  GGCGTC              
BsaI         1576  GAGACC               GGTCTC(1/5)
             2738  GGTCTC              
BseAI         632  TCCGGA               T^CCGGA
             1883  TCCGGA              
BsmBI         775  GAGACG               CGTCTC(1/5)
             2263  GAGACG              
BspEI         632  TCCGGA               T^CCGGA
             1883  TCCGGA              
BspMI        1591  ACCTGC               ACCTGC(4/8)
             2096  ACCTGC              
BsrBI        2178  CCGCTC               CCGCTC(-3/-3)
              418  GAGCGG              
BsrDI         287  CATTGC               GCAATG(2/0)
             2955  CATTGC              
CelII        1147  GCTAAGC              GC^T[ACGT]AGC
             2580  GCTGAGC             
Eco47III       47  AGCGCT               AGC^GCT
             1058  AGCGCT              
EcoT14I      2147  CCAAGG               C^C[AT][AT]GG
             2891  CCAAGG              
Hin1I        2218  GGCGTC               G[GA]^CG[CT]C
             2481  GGCGTC              
MaeII        1880  ACGT                 A^CGT
             2027  ACGT                
MluNI        2702  TGGCCA               TGG^CCA
             2903  TGGCCA              
MroI          632  TCCGGA               T^CCGGA
             1883  TCCGGA              
MscI         2702  TGGCCA               TGG^CCA
             2903  TGGCCA              
PshAI        2337  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2477  GACAGGCGTC          
SacI          904  GAGCTC               GAGCT^C
             2583  GAGCTC              
StuI          554  AGGCCT               AGG^CCT
             1727  AGGCCT              
StyI         2147  CCAAGG               C^C[AT][AT]GG
             2891  CCAAGG              
TfiI          936  GATTC                G^A[AT]TC
             1533  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI        
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