Restriction map for pF1KA0109
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BalI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssSI       Bst1107I    BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraII       DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MluNI       
MroI        MscI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI           55  GTCTAC               GT^[AC][GT]AC
AfaI         1029  GTAC                 GT^AC
AlwNI         568  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       387  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           50  CCCGAG               C^[CT]CG[GA]G
AvaII         179  GGTCC                G^G[AT]CC
AviII         128  TGCGCA               TGC^GCA
BamHI         968  GGATCC               G^GATCC
BanI         1173  GGTGCC               G^G[CT][GA]CC
BbsI          232  GTCTTC               GAAGAC(2/6)
BglI         1277  GCCGCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         232  GTCTTC               GAAGAC(2/6)
BsmAI         373  GAGAC                GTCTC(1/5)
BsoBI          50  CCCGAG               C^[CT]CG[GA]G
BsrBI         705  GAGCGG               CCGCTC(-3/-3)
BsrDI         147  CATTGC               GCAATG(2/0)
BssHII        380  GCGCGC               G^CGCGC
BstBI         235  TTCGAA               TT^CGAA
BstXI         459  CCAGGTAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         968  GGATCC               [GA]^GATC[CT]
DraI          931  TTTAAA               TTT^AAA
DrdI         1243  GACCATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          227  CCATGG               C^C[GA][CT]GG
EaeI         1275  TGGCCG               [CT]^GGCC[GA]
FspI          128  TGCGCA               TGC^GCA
HgaI          890  GACGC                GACGC(5/10)
HincII        217  GTCAAC               GT[CT]^[GA]AC
HindII        217  GTCAAC               GT[CT]^[GA]AC
MfeI          719  CAATTG               C^AATTG
MflI          968  GGATCC               [GA]^GATC[CT]
MspA1I        686  CAGCTG               C[AC]G^C[GT]G
MunI          719  CAATTG               C^AATTG
NcoI          227  CCATGG               C^CATGG
NspI          623  GCATGC               [GA]CATG^[CT]
NspV          235  TTCGAA               TT^CGAA
PvuII         686  CAGCTG               CAG^CTG
RsaI         1029  GTAC                 GT^AC
SacI          514  GAGCTC               GAGCT^C
SfcI         1236  CTACAG               C^T[GA][CT]AG
SfuI          235  TTCGAA               TT^CGAA
SmlI         1195  CTCAAG               C^T[CT][GA]AG
SphI          623  GCATGC               GCATG^C
XbaI          345  TCTAGA               T^CTAGA
XcmI          182  CCAACATTTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         968  GGATCC               [GA]^GATC[CT]
XmnI          387  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         381  CGCG                 CG^CG
             1182  CGCG                
AcsI          238  GAATTC               [GA]^AATT[CT]
              366  GAATTC              
ApoI          238  GAATTC               [GA]^AATT[CT]
              366  GAATTC              
BanII         514  GAGCTC               G[GA]GC[CT]^C
             1142  GGGCTC              
BfaI          346  CTAG                 C^TAG
              874  CTAG                
BpmI          532  CTGGAG               CTGGAG(16/14)
              901  CTGGAG              
BsaJI         227  CCATGG               C^C[ACGT][ACGT]GG
              839  CCAAGG              
BsgI          735  CTGCAC               GTGCAG(16/14)
              997  GTGCAG              
BsmFI         888  GGGAC                GGGAC(10/14)
              555  GTCCC               
BsmI          631  GAATGC               GAATGC(1/-1)
             1044  GAATGC              
BstAPI         82  GCAGTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              120  GCAGCAGGTGC         
BstNI         459  CCAGG                CC^[AT]GG
              522  CCTGG               
BstUI         381  CGCG                 CG^CG
             1182  CGCG                
Cfr13I        179  GGTCC                G^G[ACGT]CC
              591  GGGCC               
EcoRI         238  GAATTC               G^AATTC
              366  GAATTC              
EcoRII        459  CCAGG                ^CC[AT]GG
              522  CCTGG               
EcoT14I       227  CCATGG               C^C[AT][AT]GG
              839  CCAAGG              
MaeI          346  CTAG                 C^TAG
              874  CTAG                
MaeII         170  ACGT                 A^CGT
              260  ACGT                
MseI          173  TTAA                 T^TAA
              932  TTAA                
MslI          745  CACCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1155  CATTTCCATG          
MvaI          459  CCAGG                CC^[AT]GG
              522  CCTGG               
MvnI          381  CGCG                 CG^CG
             1182  CGCG                
NlaIV         968  GGATCC               GG[ACGT]^[ACGT]CC
             1173  GGTGCC              
PflMI         182  CCAACATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1049  CCATCGTGTGG         
Sau96I        179  GGTCC                G^G[ACGT]CC
              591  GGGCC               
StyI          227  CCATGG               C^C[AT][AT]GG
              839  CCAAGG              
TfiI          342  GATTC                G^A[AT]TC
             1087  GAATC               
Tru9I         173  TTAA                 T^TAA
              932  TTAA                
Van91I        182  CCAACATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1049  CCATCGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       BbvI        
BcnI        BmyI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
Cac8I       CfoI        DdeI        DpnII       EarI        
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MspI        MwoI        NciI        NdeII       
NlaIII      PleI        SapI        Sau3AI      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com