Restriction map for
pF1KA0109
(Circular) (Six-base) MAPSORT of: pF1KA0109.seq Check: 6531 from: 1 to: 4418
pF1KA0109 4418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 222 666 1098 222
Size: 444 432 3542
Fragments arranged by size:
3542 444 432
AccI GT'mk_AC
Cuts at: 164 1456 164
Size: 1292 3126
Acc65I G'GTAC_C
Cuts at: 1434 1434
Size: 4418
AclI AA'CG_TT
Cuts at: 4258 4258
Size: 4418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2675 3422 3690 2243
Size: 432 747 268 2971
Fragments arranged by size:
2971 747 432 268
AflIII A'CryG_T
Cuts at: 1770 2874 3851 1770
Size: 1104 977 2337
Fragments arranged by size:
2337 1104 977
AgeI A'CCGG_T
Cuts at: 2806 2806
Size: 4418
AleI CACnn'nnGTG
Cuts at: 857 857
Size: 4418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 4418
AlwNI CAG_nnn'CTG
Cuts at: 681 1723 3290 681
Size: 1042 1567 1809
Fragments arranged by size:
1809 1567 1042
ApaLI G'TGCA_C
Cuts at: 3188 3188
Size: 4418
ApoI r'AATT_y
Cuts at: 346 474 1422 3771 4027 346
Size: 128 948 2349 256 737
Fragments arranged by size:
2349 948 737 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 158 1438 158
Size: 1280 3138
BamHI G'GATC_C
Cuts at: 1076 1443 1076
Size: 367 4051
BanI G'GyrC_C
Cuts at: 1281 1434 1848 2096 2131 1281
Size: 153 414 248 35 3568
Fragments arranged by size:
3568 414 248 153 35
BanII G_rGCy'C
Cuts at: 626 1254 1432 2462 626
Size: 628 178 1030 2582
Fragments arranged by size:
2582 1030 628 178
BbeI G_GCGC'C
Cuts at: 1852 2100 1852
Size: 248 4170
BbsI GAAGACnn'nnnn_
Cuts at: 333 333
Size: 4418
BciVI GTATCCnnnnn_n'
Cuts at: 2316 3077 4418 2316
Size: 761 1341 2316
Fragments arranged by size:
2316 1341 761
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BglI GCCn_nnn'nGGC
Cuts at: 1391 1391
Size: 4418
BglII A'GATC_T
Cuts at: 1933 3970 1933
Size: 2037 2381
BlpI GC'TnA_GC
Cuts at: 1530 1530
Size: 4418
Bme1580I G_kGCm'C
Cuts at: 690 2043 2136 3192 690
Size: 1353 93 1056 1916
Fragments arranged by size:
1916 1353 1056 93
BmgBI CAC'GTC
Cuts at: 369 369
Size: 4418
BmrI ACTGGGnnnn_n'
Cuts at: 584 1630 1711 2046 4190 584
Size: 1046 81 335 2144 812
Fragments arranged by size:
2144 1046 812 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1030 661
Size: 369 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1594 2473 2965 3263 1288
Size: 306 879 492 298 2443
Fragments arranged by size:
2443 879 492 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 4122 4122
Size: 4418
BsaAI yAC'GTr
Cuts at: 1771 1771
Size: 4418
BsaBI GATnn'nnATC
Cuts at: 6 1932 3969 6
Size: 1926 2037 455
Fragments arranged by size:
2037 1926 455
BsaHI Gr'CG_yC
Cuts at: 1849 2097 2799 4295 1849
Size: 248 702 1496 1972
Fragments arranged by size:
1972 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1607 2128 2806 3080 3227 3638 1607
Size: 521 678 274 147 411 2387
Fragments arranged by size:
2387 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1843 2601 3178 3674 1843
Size: 758 577 496 2587
Fragments arranged by size:
2587 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 1126 828
Size: 298 4120
BsiEI CG_ry'CG
Cuts at: 104 1960 2006 3214 104
Size: 1856 46 1208 1308
Fragments arranged by size:
1856 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 152 428 626 1432 2210 2400 3192 152
Size: 276 198 806 778 190 792 1378
Fragments arranged by size:
1378 806 792 778 276 198 190
BsmI GAATG_Cn'
Cuts at: 745 1158 745
Size: 413 4005
Bsp1286I G_dGCh'C
Cuts at: 152 428 626 690 1254 1432 2043 2136 2210
Size: 276 198 64 564 178 611 93 74
Cuts at: 2210 2400 2462 3192 152
Size: 190 62 730 1378
Fragments arranged by size:
1378 730 611 564 276 198 190 178
93 74 64 62
BspEI T'CCGG_A
Cuts at: 3638 3638
Size: 4418
BspHI T'CATG_A
Cuts at: 3594 3594
Size: 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 662 666 1098 1468 1682 1984 2365 222
Size: 440 4 432 370 214 302 381 2275
Fragments arranged by size:
2275 440 432 381 370 302 214 4
BsrBI CCG'CTC
Cuts at: 50 815 2710 2764 4248 4413 50
Size: 765 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 765 165 55 54
BsrDI GCAATG_nn'
Cuts at: 254 2330 254
Size: 2076 2342
BsrFI r'CCGG_y
Cuts at: 2416 2597 2806 2416
Size: 181 209 4028
Fragments arranged by size:
4028 209 181
BssHII G'CGCG_C
Cuts at: 488 488
Size: 4418
BssSI C'ACGA_G
Cuts at: 2689 3047 2689
Size: 358 4060
BstAPI GCAn_nnn'nTGC
Cuts at: 196 234 1795 196
Size: 38 1561 2819
Fragments arranged by size:
2819 1561 38
BstBI TT'CG_AA
Cuts at: 344 2779 344
Size: 2435 1983
BstXI CCAn_nnnn'nTGG
Cuts at: 574 574
Size: 4418
BstYI r'GATC_y
Cuts at: 1 1076 1443 1910 1933 2268 2514 3526 3612
Size: 1075 367 467 23 335 246 1012 86
Cuts at: 3612 3624 3970 1
Size: 12 346 449
Fragments arranged by size:
1075 1012 467 449 367 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 335 335
Size: 4418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2368 2549 2712 2368
Size: 181 163 4074
Fragments arranged by size:
4074 181 163
BtsI GCAGTG_nn'
Cuts at: 197 1048 1395 1661 197
Size: 851 347 266 2954
Fragments arranged by size:
2954 851 347 266
DraI TTT'AAA
Cuts at: 1041 1417 1041
Size: 376 4042
DrdI GACnn_nn'nnGTC
Cuts at: 1357 2124 2982 1357
Size: 767 858 2793
Fragments arranged by size:
2793 858 767
EaeI y'GGCC_r
Cuts at: 1383 2003 2177 2568 2595 3940 1383
Size: 620 174 391 27 1345 1861
Fragments arranged by size:
1861 1345 620 391 174 27
EagI C'GGCC_G
Cuts at: 2003 2003
Size: 4418
EarI CTCTTCn'nnn_
Cuts at: 539 631 709 2441 2651 539
Size: 92 78 1732 210 2306
Fragments arranged by size:
2306 1732 210 92 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2626 2936 3082 4228 2626
Size: 310 146 1146 2816
Fragments arranged by size:
2816 1146 310 146
EcoICRI GAG'CTC
Cuts at: 624 1430 624
Size: 806 3612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1030 2243 2675 3422 3690 661
Size: 369 1213 432 747 268 1389
Fragments arranged by size:
1389 1213 747 432 369 268
EcoO109I rG'GnC_Cy
Cuts at: 1557 1557
Size: 4418
EcoRI G'AATT_C
Cuts at: 346 474 1422 346
Size: 128 948 3342
Fragments arranged by size:
3342 948 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1053 2437 1053
Size: 1384 3034
FspI TGC'GCA
Cuts at: 238 2199 238
Size: 1961 2457
HaeII r_GCGC'y
Cuts at: 1852 2100 3122 4101 1852
Size: 248 1022 979 2169
Fragments arranged by size:
2169 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 516 548 587 619 2260 2290 2292
Size: 32 323 32 39 32 1641 30 2
Cuts at: 2292 2322 161
Size: 30 2257
Fragments arranged by size:
2257 1641 323 39 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 327 1457 327
Size: 1130 3288
KasI G'GCGC_C
Cuts at: 1848 2096 1848
Size: 248 4170
KpnI G_GTAC'C
Cuts at: 1438 1438
Size: 4418
MfeI C'AATT_G
Cuts at: 827 827
Size: 4418
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 595 1058 3089 3273 314
Size: 281 463 2031 184 1459
Fragments arranged by size:
2031 1459 463 281 184
MscI TGG'CCA
Cuts at: 2179 2179
Size: 4418
MslI CAynn'nnrTG
Cuts at: 857 1267 2407 2534 857
Size: 410 1140 127 2741
Fragments arranged by size:
2741 1140 410 127
MspA1I CmG'CkG
Cuts at: 796 1529 1843 2203 3216 3461 796
Size: 733 314 360 1013 245 1753
Fragments arranged by size:
1753 1013 733 360 314 245
NaeI GCC'GGC
Cuts at: 2599 2599
Size: 4418
NarI GG'CG_CC
Cuts at: 1849 2097 1849
Size: 248 4170
NcoI C'CATG_G
Cuts at: 335 335
Size: 4418
NgoMIV G'CCGG_C
Cuts at: 2597 2597
Size: 4418
NspI r_CATG'y
Cuts at: 735 1471 1971 2878 735
Size: 736 500 907 2275
Fragments arranged by size:
2275 907 736 500
PciI A'CATG_T
Cuts at: 2874 2874
Size: 4418
PflMI CCAn_nnn'nTGG
Cuts at: 296 1163 296
Size: 867 3551
PfoI T'CCnGG_A
Cuts at: 629 629
Size: 4418
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4386
PstI C_TGCA'G
Cuts at: 1465 2150 1465
Size: 685 3733
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 796 1843 2203 796
Size: 1047 360 3011
Fragments arranged by size:
3011 1047 360
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 626 1432 626
Size: 806 3612
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SapI GCTCTTCn'nnn_
Cuts at: 539 631 709 2441 2651 539
Size: 92 78 1732 210 2306
Fragments arranged by size:
2306 1732 210 92 78
SbfI CC_TGCA'GG
Cuts at: 1465 1465
Size: 4418
ScaI AGT'ACT
Cuts at: 3935 3935
Size: 4418
SfcI C'TryA_G
Cuts at: 33 1344 1461 2146 3139 3330 33
Size: 1311 117 685 993 191 1121
Fragments arranged by size:
1311 1121 993 685 191 117
SfoI GGC'GCC
Cuts at: 1850 2098 1850
Size: 248 4170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SmaI CCC'GGG
Cuts at: 1440 1440
Size: 4418
SmlI C'TyrA_G
Cuts at: 1303 1573 2488 2980 3242 1303
Size: 270 915 492 262 2479
Fragments arranged by size:
2479 915 492 270 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 735 1471 1971 735
Size: 736 500 3182
Fragments arranged by size:
3182 736 500
StyI C'CwwG_G
Cuts at: 335 947 1552 1906 335
Size: 612 605 354 2847
Fragments arranged by size:
2847 612 605 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 2001 2134 2593 2802 1359
Size: 642 133 459 209 2975
Fragments arranged by size:
2975 642 459 209 133
TatI w'GTAC_w
Cuts at: 1136 3933 1136
Size: 2797 1621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4386
Tth111I GACn'n_nGTC
Cuts at: 2215 2215
Size: 4418
XbaI T'CTAG_A
Cuts at: 453 1449 453
Size: 996 3422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 1913 297
Size: 1616 2802
XmaI C'CCGG_G
Cuts at: 1438 1438
Size: 4418
XmnI GAAnn'nnTTC
Cuts at: 499 499
Size: 4418
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI