Restriction map for pF1KA0109
 (Linear) (Six-base) MAPSORT of: KIAA0109.seq  Check: 613  from: 1  to: 1305



KIAA0109.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     114     558     990    1305 
   Size:        114     444     432     315

  Fragments arranged by size: 

                 444     432     315     114
 
AccI GT'mk_AC

Cuts at:      0      56    1305 
   Size:         56    1249
 
AleI CACnn'nnGTG

Cuts at:      0     749    1305 
   Size:        749     556
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     737    1305 
   Size:        737     568
 
AlwNI CAG_nnn'CTG

Cuts at:      0     573    1305 
   Size:        573     732
 
ApoI r'AATT_y

Cuts at:      0     238     366    1305 
   Size:        238     128     939

  Fragments arranged by size: 

                 939     238     128
 
AvaI C'yCGr_G

Cuts at:      0      50    1305 
   Size:         50    1255
 
BamHI G'GATC_C

Cuts at:      0     968    1305 
   Size:        968     337
 
BanI G'GyrC_C

Cuts at:      0    1173    1305 
   Size:       1173     132
 
BanII G_rGCy'C

Cuts at:      0     518    1146    1305 
   Size:        518     628     159

  Fragments arranged by size: 

                 628     518     159
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     225    1305 
   Size:        225    1080
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1283    1305 
   Size:       1283      22
 
Bme1580I G_kGCm'C

Cuts at:      0     582    1305 
   Size:        582     723
 
BmgBI CAC'GTC

Cuts at:      0     261    1305 
   Size:        261    1044
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     476    1305 
   Size:        476     829
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     922    1305 
   Size:        553     369     383

  Fragments arranged by size: 

                 553     383     369
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1180    1305 
   Size:       1180     125
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     720    1018    1305 
   Size:        720     298     287

  Fragments arranged by size: 

                 720     298     287
 
BsiHKAI G_wGCw'C

Cuts at:      0      44     320     518    1305 
   Size:         44     276     198     787

  Fragments arranged by size: 

                 787     276     198      44
 
BsmI GAATG_Cn'

Cuts at:      0     637    1050    1305 
   Size:        637     413     255

  Fragments arranged by size: 

                 637     413     255
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     320     518     582    1146    1305 
   Size:         44     276     198      64     564     159

  Fragments arranged by size: 

                 564     276     198     159      64      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     114     554     558     990    1305 
   Size:        114     440       4     432     315

  Fragments arranged by size: 

                 440     432     315     114       4
 
BsrBI CCG'CTC

Cuts at:      0     707    1305 
   Size:        707     598
 
BsrDI GCAATG_nn'

Cuts at:      0     146    1305 
   Size:        146    1159
 
BssHII G'CGCG_C

Cuts at:      0     380    1305 
   Size:        380     925
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      88     126    1305 
   Size:         88      38    1179

  Fragments arranged by size: 

                1179      88      38
 
BstBI TT'CG_AA

Cuts at:      0     236    1305 
   Size:        236    1069
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     466    1305 
   Size:        466     839
 
BstYI r'GATC_y

Cuts at:      0     968    1305 
   Size:        968     337
 
BtgI C'CryG_G

Cuts at:      0     227    1305 
   Size:        227    1078
 
BtsI GCAGTG_nn'

Cuts at:      0      89     940    1287    1305 
   Size:         89     851     347      18

  Fragments arranged by size: 

                 851     347      89      18
 
DraI TTT'AAA

Cuts at:      0     933    1305 
   Size:        933     372
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1249    1305 
   Size:       1249      56
 
EaeI y'GGCC_r

Cuts at:      0    1275    1305 
   Size:       1275      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     431     523     601    1305 
   Size:        431      92      78     704

  Fragments arranged by size: 

                 704     431      92      78
 
EcoICRI GAG'CTC

Cuts at:      0     516    1305 
   Size:        516     789
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     922    1305 
   Size:        553     369     383

  Fragments arranged by size: 

                 553     383     369
 
EcoRI G'AATT_C

Cuts at:      0     238     366    1305 
   Size:        238     128     939

  Fragments arranged by size: 

                 939     238     128
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     945    1305 
   Size:        945     360
 
FspI TGC'GCA

Cuts at:      0     130    1305 
   Size:        130    1175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      53      85     408     440     479     511    1305 
   Size:         53      32     323      32      39      32     794

  Fragments arranged by size: 

                 794     323      53      39      32      32      32
 
HincII GTy'rAC

Cuts at:      0     219    1305 
   Size:        219    1086
 
MfeI C'AATT_G

Cuts at:      0     719    1305 
   Size:        719     586
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     487     950    1305 
   Size:        206     281     463     355

  Fragments arranged by size: 

                 463     355     281     206
 
MslI CAynn'nnrTG

Cuts at:      0     749    1159    1305 
   Size:        749     410     146

  Fragments arranged by size: 

                 749     410     146
 
MspA1I CmG'CkG

Cuts at:      0     688    1305 
   Size:        688     617
 
NcoI C'CATG_G

Cuts at:      0     227    1305 
   Size:        227    1078
 
NspI r_CATG'y

Cuts at:      0     627    1305 
   Size:        627     678
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     188    1055    1305 
   Size:        188     867     250

  Fragments arranged by size: 

                 867     250     188
 
PfoI T'CCnGG_A

Cuts at:      0     521    1305 
   Size:        521     784
 
PvuII CAG'CTG

Cuts at:      0     688    1305 
   Size:        688     617
 
SacI G_AGCT'C

Cuts at:      0     518    1305 
   Size:        518     787
 
SapI GCTCTTCn'nnn_

Cuts at:      0     431     523     601    1305 
   Size:        431      92      78     704

  Fragments arranged by size: 

                 704     431      92      78
 
SfcI C'TryA_G

Cuts at:      0    1236    1305 
   Size:       1236      69
 
SmlI C'TyrA_G

Cuts at:      0    1195    1305 
   Size:       1195     110
 
SphI G_CATG'C

Cuts at:      0     627    1305 
   Size:        627     678
 
StyI C'CwwG_G

Cuts at:      0     227     839    1305 
   Size:        227     612     466

  Fragments arranged by size: 

                 612     466     227
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1251    1305 
   Size:       1251      54
 
TatI w'GTAC_w

Cuts at:      0    1028    1305 
   Size:       1028     277
 
XbaI T'CTAG_A

Cuts at:      0     345    1305 
   Size:        345     960
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     189    1305 
   Size:        189    1116
 
XmnI GAAnn'nnTTC

Cuts at:      0     391    1305 
   Size:        391     914
 

 Enzymes that do cut:

     AarI     AccI     AleI     AlfI    AlwNI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI     BglI Bme1580I    BmgBI     BmrI     BpmI
    BpuEI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI
   BssHII   BstAPI    BstBI    BstXI    BstYI     BtgI     BtsI     DraI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoRI     FalI     FspI
    Hin4I   HincII     MfeI     MmeI     MslI   MspA1I     NcoI     NspI
    PflMI     PfoI    PvuII     SacI     SapI     SfcI     SmlI     SphI
     StyI    TaqII     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrFI    BsrGI    BssSI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     EagI     EciI    EcoNI EcoO109I    EcoRV
     FseI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XhoI     XmaI     ZraI
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