Restriction map for
pF1KA0094
(Circular) (Six-base) MAPSORT of: pF1KA0094.seq Check: 7288 from: 1 to: 4271
pF1KA0094 4271 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 617 2096 2528 3275 3543 527
Size: 90 1479 432 747 268 1255
Fragments arranged by size:
1479 1255 747 432 268 90
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 657 657
Size: 4271
AlwNI CAG_nnn'CTG
Cuts at: 1204 1576 3143 1204
Size: 372 1567 2332
Fragments arranged by size:
2332 1567 372
ApaLI G'TGCA_C
Cuts at: 1238 3041 1238
Size: 1803 2468
ApoI r'AATT_y
Cuts at: 1275 3624 3880 1275
Size: 2349 256 1666
Fragments arranged by size:
2349 1666 256
AseI AT'TA_AT
Cuts at: 20 326 20
Size: 306 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 1291 1291
Size: 4271
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 1287 1701 1949 1984 1287
Size: 414 248 35 3574
Fragments arranged by size:
3574 414 248 35
BanII G_rGCy'C
Cuts at: 205 1285 2315 205
Size: 1080 1030 2161
Fragments arranged by size:
2161 1080 1030
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BbsI GAAGACnn'nnnn_
Cuts at: 293 293
Size: 4271
BciVI GTATCCnnnnn_n'
Cuts at: 2169 2930 4271 2169
Size: 761 1341 2169
Fragments arranged by size:
2169 1341 761
BclI T'GATC_A
Cuts at: 435 1791 435
Size: 1356 2915
BglI GCCn_nnn'nGGC
Cuts at: 112 396 112
Size: 284 3987
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 1242 1896 1989 3045 1242
Size: 654 93 1056 2468
Fragments arranged by size:
2468 1056 654 93
BmrI ACTGGGnnnn_n'
Cuts at: 1483 1564 1899 4043 1483
Size: 81 335 2144 1711
Fragments arranged by size:
2144 1711 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 2326 2818 3116 1447
Size: 879 492 298 2602
Fragments arranged by size:
2602 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3975 3975
Size: 4271
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 1702 1950 2652 4148 1702
Size: 248 702 1496 1825
Fragments arranged by size:
1825 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 1981 2659 2933 3080 3491 1460
Size: 521 678 274 147 411 2240
Fragments arranged by size:
2240 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 189 1696 2454 3031 3527 189
Size: 1507 758 577 496 933
Fragments arranged by size:
1507 933 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 1813 1859 3067 104
Size: 12 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 843 1184 1242 1285 2063 2253 3045 843
Size: 341 58 43 778 190 792 2069
Fragments arranged by size:
2069 792 778 341 190 58 43
BsmBI CGTCTCn'nnnn_
Cuts at: 115 1148 115
Size: 1033 3238
Bsp1286I G_dGCh'C
Cuts at: 205 843 1184 1242 1285 1896 1989 2063 2253
Size: 638 341 58 43 611 93 74 190
Cuts at: 2253 2315 3045 205
Size: 62 730 1431
Fragments arranged by size:
1431 730 638 611 341 190 93 74
62 58 43
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 1321 1535 1837 2218 334
Size: 987 214 302 381 2387
Fragments arranged by size:
2387 987 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 789 2183 789
Size: 1394 2877
BsrFI r'CCGG_y
Cuts at: 568 2269 2450 2659 568
Size: 1701 181 209 2180
Fragments arranged by size:
2180 1701 209 181
BsrGI T'GTAC_A
Cuts at: 615 615
Size: 4271
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 631 1648 631
Size: 1017 3254
BstBI TT'CG_AA
Cuts at: 972 2632 972
Size: 1660 2611
BstXI CCAn_nnnn'nTGG
Cuts at: 955 1136 955
Size: 181 4090
BstYI r'GATC_y
Cuts at: 1 1296 1763 1786 2121 2367 3379 3465 3477
Size: 1295 467 23 335 246 1012 86 12
Cuts at: 3477 3823 1
Size: 346 449
Fragments arranged by size:
1295 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 444 444
Size: 4271
BtgI C'CryG_G
Cuts at: 107 116 337 714 107
Size: 9 221 377 3664
Fragments arranged by size:
3664 377 221 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 159 899 1514 159
Size: 740 615 2916
Fragments arranged by size:
2916 740 615
ClaI AT'CG_AT
Cuts at: 351 351
Size: 4271
DraI TTT'AAA
Cuts at: 231 1270 231
Size: 1039 3232
DrdI GACnn_nn'nnGTC
Cuts at: 1977 2835 1977
Size: 858 3413
EaeI y'GGCC_r
Cuts at: 113 1126 1856 2030 2421 2448 3793 113
Size: 1013 730 174 391 27 1345 591
Fragments arranged by size:
1345 1013 730 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 1856 113
Size: 1743 2528
EarI CTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1121 2479 2789 2935 4081 1121
Size: 1358 310 146 1146 1311
Fragments arranged by size:
1358 1311 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 527 617 2096 2528 3275 3543 151
Size: 376 90 1479 432 747 268 879
Fragments arranged by size:
1479 879 747 432 376 268 90
EcoO109I rG'GnC_Cy
Cuts at: 734 798 1410 734
Size: 64 612 3595
Fragments arranged by size:
3595 612 64
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
EcoRV GAT'ATC
Cuts at: 768 768
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 1705 1953 2975 3954 1705
Size: 248 1022 979 2022
Fragments arranged by size:
2022 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2113 2143 2145 2175 2113
Size: 30 2 30 4209
Fragments arranged by size:
4209 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
HindIII A'AGCT_T
Cuts at: 1000 1000
Size: 4271
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MfeI C'AATT_G
Cuts at: 1082 1082
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 801 1032 1117 2942 3126 801
Size: 231 85 1825 184 1946
Fragments arranged by size:
1946 1825 231 184 85
MscI TGG'CCA
Cuts at: 1128 2032 1128
Size: 904 3367
MslI CAynn'nnrTG
Cuts at: 867 2260 2387 867
Size: 1393 127 2751
Fragments arranged by size:
2751 1393 127
MspA1I CmG'CkG
Cuts at: 1382 1696 2056 3069 3314 1382
Size: 314 360 1013 245 2339
Fragments arranged by size:
2339 1013 360 314 245
NaeI GCC'GGC
Cuts at: 570 2452 570
Size: 1882 2389
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 107 337 714 107
Size: 230 377 3664
Fragments arranged by size:
3664 377 230
NdeI CA'TA_TG
Cuts at: 864 864
Size: 4271
NgoMIV G'CCGG_C
Cuts at: 568 2450 568
Size: 1882 2389
NspI r_CATG'y
Cuts at: 629 946 1324 1824 2731 629
Size: 317 378 500 907 2169
Fragments arranged by size:
2169 907 500 378 317
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PfoI T'CCnGG_A
Cuts at: 304 304
Size: 4271
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 4271
PsiI TTA'TAA
Cuts at: 668 668
Size: 4271
PstI C_TGCA'G
Cuts at: 151 1318 2003 151
Size: 1167 685 2419
Fragments arranged by size:
2419 1167 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 1696 2056 1696
Size: 360 3911
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SexAI A'CCwGG_T
Cuts at: 582 582
Size: 4271
SfcI C'TryA_G
Cuts at: 33 147 1314 1999 2992 3183 33
Size: 114 1167 685 993 191 1121
Fragments arranged by size:
1167 1121 993 685 191 114
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 1426 2341 2833 3095 1426
Size: 915 492 262 2602
Fragments arranged by size:
2602 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 946 1324 1824 946
Size: 378 500 3393
Fragments arranged by size:
3393 500 378
StyI C'CwwG_G
Cuts at: 107 337 714 1405 1759 107
Size: 230 377 691 354 2619
Fragments arranged by size:
2619 691 377 354 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1854 1987 2446 2655 1854
Size: 133 459 209 3470
Fragments arranged by size:
3470 459 209 133
TatI w'GTAC_w
Cuts at: 615 3786 615
Size: 3171 1100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4239
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1766 114
Size: 1652 2619
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BssHII BstEII
BstZ17I CspCI DraIII EcoNI FseI FspAI HpaI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI