Restriction map for
pF1KA0073
(Circular) (Six-base) MAPSORT of: pF1KA0073.seq Check: 5137 from: 1 to: 5051
pF1KA0073 5051 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2089 2089
Size: 5051
Acc65I G'GTAC_C
Cuts at: 2067 2067
Size: 5051
AclI AA'CG_TT
Cuts at: 4891 4891
Size: 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1378 1580 2876 3308 4055 4323 665
Size: 713 202 1296 432 747 268 1393
Fragments arranged by size:
1393 1296 747 713 432 268 202
AfeI AGC'GCT
Cuts at: 253 253
Size: 5051
AflIII A'CryG_T
Cuts at: 441 2403 3507 4484 441
Size: 1962 1104 977 1008
Fragments arranged by size:
1962 1104 1008 977
AgeI A'CCGG_T
Cuts at: 1265 3439 1265
Size: 2174 2877
AleI CACnn'nnGTG
Cuts at: 391 391
Size: 5051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 385 1367 385
Size: 982 4069
AlwNI CAG_nnn'CTG
Cuts at: 688 1498 1756 2356 3923 688
Size: 810 258 600 1567 1816
Fragments arranged by size:
1816 1567 810 600 258
ApaLI G'TGCA_C
Cuts at: 3821 3821
Size: 5051
ApoI r'AATT_y
Cuts at: 478 713 880 931 1022 1123 1789 1801 2055
Size: 235 167 51 91 101 666 12 254
Cuts at: 2055 4404 4660 478
Size: 2349 256 869
Fragments arranged by size:
2349 869 666 256 254 235 167 101
91 51 12
AseI AT'TA_AT
Cuts at: 20 1180 1184 20
Size: 1160 4 3887
Fragments arranged by size:
3887 1160 4
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
AvaI C'yCGr_G
Cuts at: 2071 2071
Size: 5051
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1880 1913 1880
Size: 33 5018
BamHI G'GATC_C
Cuts at: 1873 2076 1873
Size: 203 4848
BanI G'GyrC_C
Cuts at: 152 2067 2481 2729 2764 152
Size: 1915 414 248 35 2439
Fragments arranged by size:
2439 1915 414 248 35
BanII G_rGCy'C
Cuts at: 2065 3095 2065
Size: 1030 4021
BbeI G_GCGC'C
Cuts at: 156 2485 2733 156
Size: 2329 248 2474
Fragments arranged by size:
2474 2329 248
BbsI GAAGACnn'nnnn_
Cuts at: 818 818
Size: 5051
BciVI GTATCCnnnnn_n'
Cuts at: 2949 3710 5051 2949
Size: 761 1341 2949
Fragments arranged by size:
2949 1341 761
BclI T'GATC_A
Cuts at: 608 2571 608
Size: 1963 3088
BfrBI ATG'CAT
Cuts at: 372 372
Size: 5051
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5051
BglII A'GATC_T
Cuts at: 2566 4603 2566
Size: 2037 3014
BlpI GC'TnA_GC
Cuts at: 2163 2163
Size: 5051
Bme1580I G_kGCm'C
Cuts at: 1645 2676 2769 3825 1645
Size: 1031 93 1056 2871
Fragments arranged by size:
2871 1056 1031 93
BmrI ACTGGGnnnn_n'
Cuts at: 335 799 869 909 1872 2263 2344 2679 4823
Size: 464 70 40 963 391 81 335 2144
Cuts at: 4823 335
Size: 563
Fragments arranged by size:
2144 963 563 464 391 335 81 70
40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 3418
Size: 5051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1760 1760
Size: 5051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 2227 3106 3598 3896 1538
Size: 689 879 492 298 2693
Fragments arranged by size:
2693 879 689 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4755 4755
Size: 5051
BsaAI yAC'GTr
Cuts at: 2404 2404
Size: 5051
BsaBI GATnn'nnATC
Cuts at: 6 1499 2565 4602 6
Size: 1493 1066 2037 455
Fragments arranged by size:
2037 1493 1066 455
BsaHI Gr'CG_yC
Cuts at: 153 2482 2730 3432 4928 153
Size: 2329 248 702 1496 276
Fragments arranged by size:
2329 1496 702 276 248
BsaWI w'CCGG_w
Cuts at: 171 1265 2240 2761 3439 3713 3860 4271 171
Size: 1094 975 521 678 274 147 411 951
Fragments arranged by size:
1094 975 951 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 849 879 849
Size: 30 5021
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 749 857 1781 1799 253
Size: 496 108 924 18 3505
Fragments arranged by size:
3505 924 496 108 18
BseYI C'CCAG_C
Cuts at: 254 1233 2476 3234 3811 4307 254
Size: 979 1243 758 577 496 998
Fragments arranged by size:
1243 998 979 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1511
Size: 5051
BsiEI CG_ry'CG
Cuts at: 104 2593 2639 3847 104
Size: 2489 46 1208 1308
Fragments arranged by size:
2489 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2065 2843 3033 3825 2065
Size: 778 190 792 3291
Fragments arranged by size:
3291 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 135 135
Size: 5051
Bsp1286I G_dGCh'C
Cuts at: 1645 2065 2676 2769 2843 3033 3095 3825 1645
Size: 420 611 93 74 190 62 730 2871
Fragments arranged by size:
2871 730 611 420 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4271 4271
Size: 5051
BspHI T'CATG_A
Cuts at: 432 870 1049 4227 432
Size: 438 179 3178 1256
Fragments arranged by size:
3178 1256 438 179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2101 2315 2617 2998 2101
Size: 214 302 381 4154
Fragments arranged by size:
4154 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3343 3397 4881 5046 50
Size: 3293 54 1484 165 55
Fragments arranged by size:
3293 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 581 2963 581
Size: 2382 2669
BsrFI r'CCGG_y
Cuts at: 1265 3049 3230 3439 1265
Size: 1784 181 209 2877
Fragments arranged by size:
2877 1784 209 181
BsrGI T'GTAC_A
Cuts at: 1426 1962 1426
Size: 536 4515
BssSI C'ACGA_G
Cuts at: 3322 3680 3322
Size: 358 4693
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2428
Size: 5051
BstBI TT'CG_AA
Cuts at: 3412 3412
Size: 5051
BstXI CCAn_nnnn'nTGG
Cuts at: 1907 1907
Size: 5051
BstYI r'GATC_y
Cuts at: 1 653 1743 1873 1970 2076 2543 2566 2901
Size: 652 1090 130 97 106 467 23 335
Cuts at: 2901 3147 4159 4245 4257 4603 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1090 1012 652 467 449 346 335 246
130 106 97 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5051
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3001 3182 3345 3001
Size: 181 163 4707
Fragments arranged by size:
4707 181 163
BtsI GCAGTG_nn'
Cuts at: 218 2294 218
Size: 2076 2975
DraI TTT'AAA
Cuts at: 2050 2050
Size: 5051
DraIII CAC_nnn'GTG
Cuts at: 1215 1215
Size: 5051
DrdI GACnn_nn'nnGTC
Cuts at: 2757 3615 2757
Size: 858 4193
EaeI y'GGCC_r
Cuts at: 287 729 1128 2636 2810 3201 3228 4573 287
Size: 442 399 1508 174 391 27 1345 765
Fragments arranged by size:
1508 1345 765 442 399 391 174 27
EagI C'GGCC_G
Cuts at: 2636 2636
Size: 5051
EarI CTCTTCn'nnn_
Cuts at: 242 289 461 3074 3284 242
Size: 47 172 2613 210 2009
Fragments arranged by size:
2613 2009 210 172 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 3259 3569 3715 4861 130
Size: 3129 310 146 1146 320
Fragments arranged by size:
3129 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 2063 2063
Size: 5051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1378 1580 1760 2876 3308 4055 4323 665
Size: 713 202 180 1116 432 747 268 1393
Fragments arranged by size:
1393 1116 747 713 432 268 202 180
EcoO109I rG'GnC_Cy
Cuts at: 159 1564 2190 159
Size: 1405 626 3020
Fragments arranged by size:
3020 1405 626
EcoRI G'AATT_C
Cuts at: 2055 2055
Size: 5051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 5051
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5051
HaeII r_GCGC'y
Cuts at: 156 255 2485 2733 3755 4734 156
Size: 99 2230 248 1022 979 473
Fragments arranged by size:
2230 1022 979 473 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 849 881 1728 1760 2893 2923 2925
Size: 32 449 32 847 32 1133 30 2
Cuts at: 2925 2955 368
Size: 30 2464
Fragments arranged by size:
2464 1133 847 449 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2090 2090
Size: 5051
HindIII A'AGCT_T
Cuts at: 1373 1373
Size: 5051
KasI G'GCGC_C
Cuts at: 152 2481 2729 152
Size: 2329 248 2474
Fragments arranged by size:
2474 2329 248
KpnI G_GTAC'C
Cuts at: 2071 2071
Size: 5051
MfeI C'AATT_G
Cuts at: 1312 1312
Size: 5051
MluI A'CGCG_T
Cuts at: 4484 4484
Size: 5051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 240 979 1136 1771 2000 3722 3906 240
Size: 739 157 635 229 1722 184 1385
Fragments arranged by size:
1722 1385 739 635 229 184 157
MscI TGG'CCA
Cuts at: 289 2812 289
Size: 2523 2528
MslI CAynn'nnrTG
Cuts at: 377 391 1603 3040 3167 377
Size: 14 1212 1437 127 2261
Fragments arranged by size:
2261 1437 1212 127 14
MspA1I CmG'CkG
Cuts at: 2162 2476 2836 3849 4094 2162
Size: 314 360 1013 245 3119
Fragments arranged by size:
3119 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 5051
NarI GG'CG_CC
Cuts at: 153 2482 2730 153
Size: 2329 248 2474
Fragments arranged by size:
2474 2329 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5051
NdeI CA'TA_TG
Cuts at: 1777 1777
Size: 5051
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 5051
NsiI A_TGCA'T
Cuts at: 374 374
Size: 5051
NspI r_CATG'y
Cuts at: 372 445 618 1080 2104 2604 3511 372
Size: 73 173 462 1024 500 907 1912
Fragments arranged by size:
1912 1024 907 500 462 173 73
PacI TTA_AT'TAA
Cuts at: 1184 1184
Size: 5051
PasI CC'CwG_GG
Cuts at: 664 664
Size: 5051
PciI A'CATG_T
Cuts at: 441 3507 441
Size: 3066 1985
PfoI T'CCnGG_A
Cuts at: 636 636
Size: 5051
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 5019
PpuMI rG'GwC_Cy
Cuts at: 159 1564 159
Size: 1405 3646
PsiI TTA'TAA
Cuts at: 1249 1691 1249
Size: 442 4609
PstI C_TGCA'G
Cuts at: 2098 2783 2098
Size: 685 4366
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5051
PvuII CAG'CTG
Cuts at: 2476 2836 2476
Size: 360 4691
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5051
SacI G_AGCT'C
Cuts at: 2065 2065
Size: 5051
SalI G'TCGA_C
Cuts at: 2088 2088
Size: 5051
SanDI GG'GwC_CC
Cuts at: 159 159
Size: 5051
SapI GCTCTTCn'nnn_
Cuts at: 242 3074 3284 242
Size: 2832 210 2009
Fragments arranged by size:
2832 2009 210
SbfI CC_TGCA'GG
Cuts at: 2098 2098
Size: 5051
ScaI AGT'ACT
Cuts at: 4568 4568
Size: 5051
SfcI C'TryA_G
Cuts at: 33 1142 1361 2094 2779 3772 3963 33
Size: 1109 219 733 685 993 191 1121
Fragments arranged by size:
1121 1109 993 733 685 219 191
SfoI GGC'GCC
Cuts at: 154 2483 2731 154
Size: 2329 248 2474
Fragments arranged by size:
2474 2329 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
SmaI CCC'GGG
Cuts at: 2073 2073
Size: 5051
SmlI C'TyrA_G
Cuts at: 1553 2206 3121 3613 3875 1553
Size: 653 915 492 262 2729
Fragments arranged by size:
2729 915 653 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5051
SphI G_CATG'C
Cuts at: 2104 2604 2104
Size: 500 4551
SspI AAT'ATT
Cuts at: 1299 1401 2034 1299
Size: 102 633 4316
Fragments arranged by size:
4316 633 102
StyI C'CwwG_G
Cuts at: 107 1906 2185 2539 107
Size: 1799 279 354 2619
Fragments arranged by size:
2619 1799 354 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 403 550 2634 2767 3226 3435 403
Size: 147 2084 133 459 209 2019
Fragments arranged by size:
2084 2019 459 209 147 133
TatI w'GTAC_w
Cuts at: 703 1426 1962 4566 703
Size: 723 536 2604 1188
Fragments arranged by size:
2604 1188 723 536
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1199 1231 4568 4600 1199
Size: 32 3337 32 1650
Fragments arranged by size:
3337 1650 32 32
Tth111I GACn'n_nGTC
Cuts at: 2848 2848
Size: 5051
XbaI T'CTAG_A
Cuts at: 2082 2082
Size: 5051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5051
XmaI C'CCGG_G
Cuts at: 2071 2071
Size: 5051
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI NheI NotI NruI PflMI PmlI PshAI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI