Restriction map for pF1KA0060
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MfeI        MflI        MluI        MroI        MspA1I      
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SapI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TfiI        
Tsp509I     XbaI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          334  GTCGAC               GT^[AC][GT]AC
AflIII        665  ACATGT               A^C[GA][CT]GT
AspI          672  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          233  CTCGAG               C^[CT]CG[GA]G
BanI          540  GGTGCC               G^G[CT][GA]CC
BanII         112  GGGCTC               G[GA]GC[CT]^C
BciVI         492  GGATAC               GTATCC(6/5)
BcnI          243  CCCGG                CC^[GC]GG
BsaAI         220  TACGTG               [CT]AC^GT[GA]
BsaBI         600  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          236  GAGACC               GGTCTC(1/5)
BseRI         649  GAGGAG               GAGGAG(10/8)
BsmAI         236  GAGAC                GTCTC(1/5)
BsmFI         176  GGGAC                GGGAC(10/14)
BsmI          327  GAATGC               GAATGC(1/-1)
BsoBI         233  CTCGAG               C^[CT]CG[GA]G
BspLU11I      665  ACATGT               A^CATGT
BspMI         382  GCAGGT               ACCTGC(4/8)
BsrDI         429  CATTGC               GCAATG(2/0)
BstBI         846  TTCGAA               TT^CGAA
DraI          187  TTTAAA               TTT^AAA
EarI          364  GAAGAG               CTCTTC(1/4)
FauI          698  CCCGC                CCCGC(4/6)
HapII         244  CCGG                 C^CGG
HgaI          480  GACGC                GACGC(5/10)
HincII        334  GTCGAC               GT[CT]^[GA]AC
HindII        334  GTCGAC               GT[CT]^[GA]AC
HpaII         244  CCGG                 C^CGG
Ksp632I       364  GAAGAG               CTCTTC(1/4)
MaeII         221  ACGT                 A^CGT
MamI          600  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI          244  CCGG                 C^CGG
MwoI          616  GCTCACAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          243  CCCGG                CC^[GC]GG
NspI          665  ACATGT               [GA]CATG^[CT]
NspV          846  TTCGAA               TT^CGAA
PaeR7I        233  CTCGAG               C^TCGAG
PflFI         672  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         312  CCACATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          380  CTGCAG               CTGCA^G
SalI          334  GTCGAC               G^TCGAC
ScaI           98  AGTACT               AGT^ACT
SfuI          846  TTCGAA               TT^CGAA
SmlI          233  CTCGAG               C^T[CT][GA]AG
Tsp45I        716  GTGAC                ^GT[GC]AC
Tth111I       672  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        312  CCACATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          312  CCACATTCTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          233  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          699  CCGC                 CCGC(-3/-1)
               46  GCGG                
AlwI          600  GATCC                GGATC(4/5)
              389  GGATC               
AspHI          19  GAGCAC               G[AT]GC[AT]^C
              614  GTGCTC              
BalI          485  TGGCCA               TGG^CCA
              503  TGGCCA              
BfaI          512  CTAG                 C^TAG
              587  CTAG                
BpmI          450  CTCCAG               CTGGAG(16/14)
               16  CTGGAG              
BsiHKAI        19  GAGCAC               G[AT]GC[AT]^C
              614  GTGCTC              
Bsp1407I      635  TGTACA               T^GTACA
              806  TGTACA              
BsrGI         635  TGTACA               T^GTACA
              806  TGTACA              
Cac8I          34  GCGAGC               GC[ACGT]^[ACGT]GC
              483  GCTGGC              
DdeI           29  CTCAG                C^T[ACGT]AG
              830  CTGAG               
DraII         176  GGGACCT              [GA]G^G[ACGT]CC[CT]
              466  AGGACCC             
DsaI          488  CCATGG               C^C[GA][CT]GG
              548  CCATGG              
EaeI          485  TGGCCA               [CT]^GGCC[GA]
              503  TGGCCA              
EcoO109I      176  GGGACCT              [GA]G^G[ACGT]CC[CT]
              466  AGGACCC             
MaeI          512  CTAG                 C^TAG
              587  CTAG                
MaeIII        251  GTTAC                ^GT[ACGT]AC
              716  GTGAC               
MluNI         485  TGGCCA               TGG^CCA
              503  TGGCCA              
MscI          485  TGGCCA               TGG^CCA
              503  TGGCCA              
MslI          534  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              573  CACTGTCATG          
NcoI          488  CCATGG               C^CATGG
              548  CCATGG              
PpuMI         176  GGGACCT              [GA]G^G[AT]CC[CT]
              466  AGGACCC             
SfcI          340  CTACAG               C^T[GA][CT]AG
              380  CTGCAG              
SspBI         635  TGTACA               T^GTACA
              806  TGTACA              
TspRI         120  CACTG                CA[GC]TG[ACGT][ACGT]^
              573  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AfaI        AluI        Asp700I     AvaII       BbsI        
BbvI        BmyI        BpuAI       BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DpnII       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MboI        MboII       MnlI        MseI        MvaI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
Tru9I       TseI        TthHB8I     XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com