Restriction map for pF1KA0018
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AocI        ApaI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BalI        BamHI       
BbvCI       BciVI       BfrI        BlnI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrFI       BsrGI       Bst1107I    BstBI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoRI       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI         1219  GTCTAC               GT^[AC][GT]AC
AflIII        306  ACGTGT               A^C[GA][CT]GT
AlwI          684  GATCC                GGATC(4/5)
Asp700I       789  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1510  CCCGAG               C^[CT]CG[GA]G
AviII        1121  TGCGCA               TGC^GCA
BbeI          278  GGCGCC               GGCGC^C
BbrPI        1144  CACGTG               CAC^GTG
BcgI          509  GCACAGGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1431  CCGGG                CC^[GC]GG
BfaI         1267  CTAG                 C^TAG
BglI         1256  GCCAGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          177  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      177  GCTCAGC              GC^T[ACGT]AGC
BsaBI         684  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1114  GAGACC               GGTCTC(1/5)
BseRI        1435  GAGGAG               GAGGAG(10/8)
BsoBI        1510  CCCGAG               C^[CT]CG[GA]G
BspMI         228  GCAGGT               ACCTGC(4/8)
BsrBI         121  CCGCTC               CCGCTC(-3/-3)
BsrI          730  CCAGT                ACTGG(1/-1)
BstEII        408  GGTGACC              G^GT[ACGT]ACC
BstPI         408  GGTGACC              G^GT[ACGT]ACC
CelII         177  GCTCAGC              GC^T[ACGT]AGC
DrdI         1210  GACATCCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       408  GGTGACC              G^GT[ACGT]ACC
EcoRV         139  GATATC               GAT^ATC
FspI         1121  TGCGCA               TGC^GCA
HapII        1431  CCGG                 C^CGG
HpaII        1431  CCGG                 C^CGG
KasI          278  GGCGCC               G^GCGCC
MaeI         1267  CTAG                 C^TAG
MamI          684  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          914  TTAA                 T^TAA
MslI         1158  CATGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1431  CCGG                 C^CGG
NarI          278  GGCGCC               GG^CGCC
NciI         1431  CCGGG                CC^[GC]GG
NcoI          413  CCATGG               C^CATGG
NdeI         1316  CATATG               CA^TATG
PmaCI        1144  CACGTG               CAC^GTG
PmlI         1144  CACGTG               CAC^GTG
PpuMI        1351  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         840  GACAGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1180  CTGCAG               CTGCA^G
PvuII        1366  CAGCTG               CAG^CTG
SapI           36  GCTCTTC              GCTCTTC(1/4)
SfcI         1180  CTGCAG               C^T[GA][CT]AG
SfoI          278  GGCGCC               GGC^GCC
SmlI          969  CTTGAG               C^T[CT][GA]AG
StuI         1263  AGGCCT               AGG^CCT
Tru9I         914  TTAA                 T^TAA
Tsp509I       892  AATT                 ^AATT
XmnI          789  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          278  GGCGCC               G[GA]^CG[CT]C
             1501  GACGCC              
Alw44I        270  GTGCAC               G^TGCAC
             1270  GTGCAC              
Aor51HI        89  AGCGCT               AGC^GCT
              184  AGCGCT              
ApaLI         270  GTGCAC               G^TGCAC
             1270  GTGCAC              
BbsI          324  GAAGAC               GAAGAC(2/6)
              936  GAAGAC              
BclI          344  TGATCA               T^GATCA
              500  TGATCA              
BglII        1085  AGATCT               A^GATCT
             1526  AGATCT              
BpuAI         324  GAAGAC               GAAGAC(2/6)
              936  GAAGAC              
BsaHI         278  GGCGCC               G[GA]^CG[CT]C
             1501  GACGCC              
BspHI         341  TCATGA               T^CATGA
              848  TCATGA              
BssHII         29  GCGCGC               G^CGCGC
              156  GCGCGC              
BssSI         199  CACGAG               CACGAG(-5/-1)
              769  CACGAG              
DsaI          413  CCATGG               C^C[GA][CT]GG
              904  CCGTGG              
EaeI           20  TGGCCG               [CT]^GGCC[GA]
              674  TGGCCG              
EarI           37  CTCTTC               CTCTTC(1/4)
              109  CTCTTC              
Eco47III       89  AGCGCT               AGC^GCT
              184  AGCGCT              
EcoT14I       404  CCTTGG               C^C[AT][AT]GG
              413  CCATGG              
FbaI          344  TGATCA               T^GATCA
              500  TGATCA              
HgaI          657  GACGC                GACGC(5/10)
             1501  GACGC               
Hin1I         278  GGCGCC               G[GA]^CG[CT]C
             1501  GACGCC              
Ksp632I        37  CTCTTC               CTCTTC(1/4)
              109  CTCTTC              
MaeIII        409  GTGAC                ^GT[ACGT]AC
              424  GTGAC               
NspI          918  GCATGT               [GA]CATG^[CT]
             1157  ACATGC              
PflMI         443  CCATTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              792  CCACTTCGTGG         
RcaI          341  TCATGA               T^CATGA
              848  TCATGA              
SacI         1012  GAGCTC               GAGCT^C
             1294  GAGCTC              
StyI          404  CCTTGG               C^C[AT][AT]GG
              413  CCATGG              
Tsp45I        409  GTGAC                ^GT[GC]AC
              424  GTGAC               
Van91I        443  CCATTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              792  CCACTTCGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BanI        BanII       BbvI        
BmyI        BpmI        BsaAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DraIII      EcoNI       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeII       MboI        MboII       
MflI        MnlI        MspA1I      MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TspRI       TthHB8I     XhoII       
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