Restriction map for pF1KA0018
 (Circular) (Six-base) MAPSORT of: pF1KA0018.seq  Check: 3574  from: 1  to: 4661



pF1KA0018  4661 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    327     327 
   Size:       4661
 
AccI GT'mk_AC

Cuts at:   1328    1699    1328 
   Size:        371    4290
 
Acc65I G'GTAC_C

Cuts at:   1677    1677 
   Size:       4661
 
AclI AA'CG_TT

Cuts at:   4501    4501 
   Size:       4661
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    187    1063    1225    2486    2918    3665    3933     187 
   Size:        876     162    1261     432     747     268     915

  Fragments arranged by size: 

                1261     915     876     747     432     268     162
 
AfeI AGC'GCT

Cuts at:    199     294     199 
   Size:         95    4566
 
AflIII A'CryG_T

Cuts at:    414    2013    3117    4094     414 
   Size:       1599    1104     977     981

  Fragments arranged by size: 

                1599    1104     981     977
 
AgeI A'CCGG_T

Cuts at:   3049    3049 
   Size:       4661
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    721     721 
   Size:       4661
 
AlwNI CAG_nnn'CTG

Cuts at:   1299    1463    1515    1966    3533    1299 
   Size:        164      52     451    1567    2427

  Fragments arranged by size: 

                2427    1567     451     164      52
 
ApaLI G'TGCA_C

Cuts at:    378    1378    3431     378 
   Size:       1000    2053    1608

  Fragments arranged by size: 

                2053    1608    1000
 
ApoI r'AATT_y

Cuts at:   1665    4014    4270    1665 
   Size:       2349     256    2056

  Fragments arranged by size: 

                2349    2056     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4661
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4661
 
AvaI C'yCGr_G

Cuts at:   1618    1681    1618 
   Size:         63    4598
 
BamHI G'GATC_C

Cuts at:   1686    1686 
   Size:       4661
 
BanI G'GyrC_C

Cuts at:    386    1182    1272    1677    2091    2339    2374     386 
   Size:        796      90     405     414     248      35    2673

  Fragments arranged by size: 

                2673     796     414     405     248      90      35
 
BanII G_rGCy'C

Cuts at:    116     512     977    1124    1406    1675    2705     116 
   Size:        396     465     147     282     269    1030    2072

  Fragments arranged by size: 

                2072    1030     465     396     282     269     147
 
BbeI G_GCGC'C

Cuts at:    390    2095    2343     390 
   Size:       1705     248    2708

  Fragments arranged by size: 

                2708    1705     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    439    1051     439 
   Size:        612    4049
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    606     640     606 
   Size:         34    4627
 
BciVI GTATCCnnnnn_n'

Cuts at:   2559    3320    4661    2559 
   Size:        761    1341    2559

  Fragments arranged by size: 

                2559    1341     761
 
BclI T'GATC_A

Cuts at:    452     608    2181     452 
   Size:        156    1573    2932

  Fragments arranged by size: 

                2932    1573     156
 
BglI GCCn_nnn'nGGC

Cuts at:   1370    1370 
   Size:       4661
 
BglII A'GATC_T

Cuts at:   1193    1634    2176    4213    1193 
   Size:        441     542    2037    1641

  Fragments arranged by size: 

                2037    1641     542     441
 
BlpI GC'TnA_GC

Cuts at:    286    1773     286 
   Size:       1487    3174
 
Bme1580I G_kGCm'C

Cuts at:    382     619    1277    1382    2286    2379    3435     382 
   Size:        237     658     105     904      93    1056    1608

  Fragments arranged by size: 

                1608    1056     904     658     237     105      93
 
BmgBI CAC'GTC

Cuts at:   1326    1326 
   Size:       4661
 
BmrI ACTGGGnnnn_n'

Cuts at:   1873    1954    2289    4433    1873 
   Size:         81     335    2144    2101

  Fragments arranged by size: 

                2144    2101     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3028    3028 
   Size:       4661
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    196     871    1084    1108    1498     196 
   Size:        675     213      24     390    3359

  Fragments arranged by size: 

                3359     675     390     213      24
 
Bpu10I CC'TnA_GC

Cuts at:    578     578 
   Size:       4661
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1098    1837    2716    3208    3506    1098 
   Size:        739     879     492     298    2253

  Fragments arranged by size: 

                2253     879     739     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1216    4365    1216 
   Size:       3149    1512
 
BsaAI yAC'GTr

Cuts at:    261     415    1254    2014     261 
   Size:        154     839     760    2908

  Fragments arranged by size: 

                2908     839     760     154
 
BsaBI GATnn'nnATC

Cuts at:      6     796    2175    4212       6 
   Size:        790    1379    2037     455

  Fragments arranged by size: 

                2037    1379     790     455
 
BsaHI Gr'CG_yC

Cuts at:    387    1610    2092    2340    3042    4538     387 
   Size:       1223     482     248     702    1496     510

  Fragments arranged by size: 

                1496    1223     702     510     482     248
 
BsaWI w'CCGG_w

Cuts at:   1850    2371    3049    3323    3470    3881    1850 
   Size:        521     678     274     147     411    2630

  Fragments arranged by size: 

                2630     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1558    1558 
   Size:       4661
 
BseYI C'CCAG_C

Cuts at:    200     768     884     976    1360    1596    2086    2844    3421 
   Size:        568     116      92     384     236     490     758     577

Cuts at:   3421    3917     200 
   Size:        496     944

  Fragments arranged by size: 

                 944     758     577     568     496     490     384     236
                 116      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    289     651    1279    1285     289 
   Size:        362     628       6    3665

  Fragments arranged by size: 

                3665     628     362       6
 
BsiEI CG_ry'CG

Cuts at:    104    2203    2249    3457     104 
   Size:       2099      46    1208    1308

  Fragments arranged by size: 

                2099    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    188     382    1124    1382    1406    1675    2453    2643    3435 
   Size:        194     742     258      24     269     778     190     792

Cuts at:   3435     188 
   Size:       1414

  Fragments arranged by size: 

                1414     792     778     742     269     258     194     190
                  24
 
Bsp1286I G_dGCh'C

Cuts at:    116     188     382     512     619     977    1124    1277    1382 
   Size:         72     194     130     107     358     147     153     105

Cuts at:   1382    1406    1675    2286    2379    2453    2643    2705    3435 
   Size:         24     269     611      93      74     190      62     730

Cuts at:   3435     116 
   Size:       1342

  Fragments arranged by size: 

                1342     730     611     358     269     194     190     153
                 147     130     107     105      93      74      72      62
                  24
 
BspEI T'CCGG_A

Cuts at:   3881    3881 
   Size:       4661
 
BspHI T'CATG_A

Cuts at:    449     956    3837     449 
   Size:        507    2881    1273

  Fragments arranged by size: 

                2881    1273     507
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    327    1711    1925    2227    2608     327 
   Size:       1384     214     302     381    2380

  Fragments arranged by size: 

                2380    1384     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     231    2953    3007    4491    4656      50 
   Size:        181    2722      54    1484     165      55

  Fragments arranged by size: 

                2722    1484     181     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2573    2573 
   Size:       4661
 
BsrFI r'CCGG_y

Cuts at:   2659    2840    3049    2659 
   Size:        181     209    4271

  Fragments arranged by size: 

                4271     209     181
 
BssHII G'CGCG_C

Cuts at:    137     264     137 
   Size:        127    4534
 
BssSI C'ACGA_G

Cuts at:    307     877    2932    3290     307 
   Size:        570    2055     358    1678

  Fragments arranged by size: 

                2055    1678     570     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    318    1266    1299    2038     318 
   Size:        948      33     739    2941

  Fragments arranged by size: 

                2941     948     739      33
 
BstBI TT'CG_AA

Cuts at:   3022    3022 
   Size:       4661
 
BstEII G'GTnAC_C

Cuts at:    516     516 
   Size:       4661
 
BstYI r'GATC_y

Cuts at:      1     791    1193    1634    1686    2153    2176    2511    2757 
   Size:        790     402     441      52     467      23     335     246

Cuts at:   2757    3769    3855    3867    4213       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     790     467     449     441     402     346     335
                 246      86      52      23      12
 
BtgI C'CryG_G

Cuts at:    107     521    1012     107 
   Size:        414     491    3756

  Fragments arranged by size: 

                3756     491     414
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    723    2611    2792    2955     723 
   Size:       1888     181     163    2429

  Fragments arranged by size: 

                2429    1888     181     163
 
BtsI GCAGTG_nn'

Cuts at:    668    1904     668 
   Size:       1236    3425
 
DraI TTT'AAA

Cuts at:   1660    1660 
   Size:       4661
 
DraIII CAC_nnn'GTG

Cuts at:    415     906    1254    1257     415 
   Size:        491     348       3    3819

  Fragments arranged by size: 

                3819     491     348       3
 
DrdI GACnn_nn'nnGTC

Cuts at:   1324    2367    3225    1324 
   Size:       1043     858    2760

  Fragments arranged by size: 

                2760    1043     858
 
EaeI y'GGCC_r

Cuts at:    128     782    2246    2420    2811    2838    4183     128 
   Size:        654    1464     174     391      27    1345     606

  Fragments arranged by size: 

                1464    1345     654     606     391     174      27
 
EagI C'GGCC_G

Cuts at:   2246    2246 
   Size:       4661
 
EarI CTCTTCn'nnn_

Cuts at:    151     223    2684    2894     151 
   Size:         72    2461     210    1918

  Fragments arranged by size: 

                2461    1918     210      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2869    3179    3325    4471    2869 
   Size:        310     146    1146    3059

  Fragments arranged by size: 

                3059    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1122    1404    1673    1122 
   Size:        282     269    4110

  Fragments arranged by size: 

                4110     282     269
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    187     196     871    1063    1084    1108    1225    1498    2486 
   Size:          9     675     192      21      24     117     273     988

Cuts at:   2486    2918    3665    3933     187 
   Size:        432     747     268     915

  Fragments arranged by size: 

                 988     915     747     675     432     273     268     192
                 117      24      21       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:    931    1291    1467     931 
   Size:        360     176    4125

  Fragments arranged by size: 

                4125     360     176
 
EcoO109I rG'GnC_Cy

Cuts at:    846    1059    1296    1460    1800     846 
   Size:        213     237     164     340    3707

  Fragments arranged by size: 

                3707     340     237     213     164
 
EcoRI G'AATT_C

Cuts at:   1665    1665 
   Size:       4661
 
EcoRV GAT'ATC

Cuts at:    249     249 
   Size:       4661
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2680    2680 
   Size:       4661
 
FspI TGC'GCA

Cuts at:   1231    2442    1231 
   Size:       1211    3450
 
HaeII r_GCGC'y

Cuts at:    201     296     390    2095    2343    3365    4344     201 
   Size:         95      94    1705     248    1022     979     518

  Fragments arranged by size: 

                1705    1022     979     518     248      95      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    619     651    1170    1202    2503    2533    2535    2565     619 
   Size:         32     519      32    1301      30       2      30    2715

  Fragments arranged by size: 

                2715    1301     519      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1700    1700 
   Size:       4661
 
KasI G'GCGC_C

Cuts at:    386    2091    2339     386 
   Size:       1705     248    2708

  Fragments arranged by size: 

                2708    1705     248
 
KpnI G_GTAC'C

Cuts at:   1681    1681 
   Size:       4661
 
MluI A'CGCG_T

Cuts at:   4094    4094 
   Size:       4661
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    681    3332    3516     681 
   Size:       2651     184    1826

  Fragments arranged by size: 

                2651    1826     184
 
MscI TGG'CCA

Cuts at:   2422    2422 
   Size:       4661
 
MslI CAynn'nnrTG

Cuts at:   1270    2650    2777    1270 
   Size:       1380     127    3154

  Fragments arranged by size: 

                3154    1380     127
 
MspA1I CmG'CkG

Cuts at:    787     888    1476    1772    2086    2446    3459    3704     787 
   Size:        101     588     296     314     360    1013     245    1744

  Fragments arranged by size: 

                1744    1013     588     360     314     296     245     101
            
 
NaeI GCC'GGC

Cuts at:   2842    2842 
   Size:       4661
 
NarI GG'CG_CC

Cuts at:    387    2092    2340     387 
   Size:       1705     248    2708

  Fragments arranged by size: 

                2708    1705     248
 
NcoI C'CATG_G

Cuts at:    107     521     107 
   Size:        414    4247
 
NdeI CA'TA_TG

Cuts at:   1425    1425 
   Size:       4661
 
NgoMIV G'CCGG_C

Cuts at:   2840    2840 
   Size:       4661
 
NspI r_CATG'y

Cuts at:   1030    1269    1714    2214    3121    1030 
   Size:        239     445     500     907    2570

  Fragments arranged by size: 

                2570     907     500     445     239
 
PasI CC'CwG_GG

Cuts at:   1215    1215 
   Size:       4661
 
PciI A'CATG_T

Cuts at:   3117    3117 
   Size:       4661
 
PflMI CCAn_nnn'nTGG

Cuts at:    557     906     557 
   Size:        349    4312
 
PfoI T'CCnGG_A

Cuts at:   1124    1124 
   Size:       4661
 
PmeI GTTT'AAAC

Cuts at:   1660    1660 
   Size:       4661
 
PmlI CAC'GTG

Cuts at:   1254    1254 
   Size:       4661
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3800    3832    3800 
   Size:         32    4629
 
PpuMI rG'GwC_Cy

Cuts at:   1460    1460 
   Size:       4661
 
PshAI GACnn'nnGTC

Cuts at:    952     952 
   Size:       4661
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    637     669     637 
   Size:         32    4629
 
PstI C_TGCA'G

Cuts at:   1292    1708    2393    1292 
   Size:        416     685    3560

  Fragments arranged by size: 

                3560     685     416
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4661
 
PvuII CAG'CTG

Cuts at:   1476    2086    2446    1476 
   Size:        610     360    3691

  Fragments arranged by size: 

                3691     610     360
 
RsrII CG'GwC_CG

Cuts at:   2856    2856 
   Size:       4661
 
SacI G_AGCT'C

Cuts at:   1124    1406    1675    1124 
   Size:        282     269    4110

  Fragments arranged by size: 

                4110     282     269
 
SalI G'TCGA_C

Cuts at:   1698    1698 
   Size:       4661
 
SapI GCTCTTCn'nnn_

Cuts at:    151    2684    2894     151 
   Size:       2533     210    1918

  Fragments arranged by size: 

                2533    1918     210
 
SbfI CC_TGCA'GG

Cuts at:   1708    1708 
   Size:       4661
 
ScaI AGT'ACT

Cuts at:   4178    4178 
   Size:       4661
 
SfcI C'TryA_G

Cuts at:     33    1288    1704    2389    3382    3573      33 
   Size:       1255     416     685     993     191    1121

  Fragments arranged by size: 

                1255    1121     993     685     416     191
 
SfoI GGC'GCC

Cuts at:    388    2093    2341     388 
   Size:       1705     248    2708

  Fragments arranged by size: 

                2708    1705     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4661
 
SmaI CCC'GGG

Cuts at:   1683    1683 
   Size:       4661
 
SmlI C'TyrA_G

Cuts at:   1077    1816    2731    3223    3485    1077 
   Size:        739     915     492     262    2253

  Fragments arranged by size: 

                2253     915     739     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4661
 
SphI G_CATG'C

Cuts at:   1714    2214    1714 
   Size:        500    4161
 
StuI AGG'CCT

Cuts at:   1373    1373 
   Size:       4661
 
StyI C'CwwG_G

Cuts at:    107     512     521    1795    2149     107 
   Size:        405       9    1274     354    2619

  Fragments arranged by size: 

                2619    1274     405     354       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    147     192     261     889    2244    2377    2836    3045     147 
   Size:         45      69     628    1355     133     459     209    1763

  Fragments arranged by size: 

                1763    1355     628     459     209     133      69      45
            
 
TatI w'GTAC_w

Cuts at:    425    1067    4176     425 
   Size:        642    3109     910

  Fragments arranged by size: 

                3109     910     642
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4178    4210    4178 
   Size:         32    4629
 
Tth111I GACn'n_nGTC

Cuts at:   2458    2458 
   Size:       4661
 
XbaI T'CTAG_A

Cuts at:   1692    1692 
   Size:       4661
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2156    2156 
   Size:       4661
 
XmaI C'CCGG_G

Cuts at:   1681    1681 
   Size:       4661
 
XmnI GAAnn'nnTTC

Cuts at:    901     901 
   Size:       4661
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AleI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI    BfrBI     BmtI    BsaXI    BsiWI     BsmI    BsmBI
    BsrGI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     FseI    FspAI
  HindIII     HpaI     MfeI     NheI     NotI     NruI     NsiI     PacI
     PsiI   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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