Restriction map for pF1KA0018
 (Linear) (Six-base) MAPSORT of: KIAA0018.seq  Check: 7015  from: 1  to: 1548



KIAA0018.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     219    1548 
   Size:        219    1329
 
AccI GT'mk_AC

Cuts at:      0    1220    1548 
   Size:       1220     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     955    1117    1548 
   Size:         79     876     162     431

  Fragments arranged by size: 

                 876     431     162      79
 
AfeI AGC'GCT

Cuts at:      0      91     186    1548 
   Size:         91      95    1362

  Fragments arranged by size: 

                1362      95      91
 
AflIII A'CryG_T

Cuts at:      0     306    1548 
   Size:        306    1242
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     613    1548 
   Size:        613     935
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1191    1355    1407    1548 
   Size:       1191     164      52     141

  Fragments arranged by size: 

                1191     164     141      52
 
ApaLI G'TGCA_C

Cuts at:      0     270    1270    1548 
   Size:        270    1000     278

  Fragments arranged by size: 

                1000     278     270
 
AvaI C'yCGr_G

Cuts at:      0    1510    1548 
   Size:       1510      38
 
BanI G'GyrC_C

Cuts at:      0     278    1074    1164    1548 
   Size:        278     796      90     384

  Fragments arranged by size: 

                 796     384     278      90
 
BanII G_rGCy'C

Cuts at:      0       8     404     869    1016    1298    1548 
   Size:          8     396     465     147     282     250

  Fragments arranged by size: 

                 465     396     282     250     147       8
 
BbeI G_GCGC'C

Cuts at:      0     282    1548 
   Size:        282    1266
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     331     943    1548 
   Size:        331     612     605

  Fragments arranged by size: 

                 612     605     331
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     498     532    1548 
   Size:        498      34    1016

  Fragments arranged by size: 

                1016     498      34
 
BclI T'GATC_A

Cuts at:      0     344     500    1548 
   Size:        344     156    1048

  Fragments arranged by size: 

                1048     344     156
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1262    1548 
   Size:       1262     286
 
BglII A'GATC_T

Cuts at:      0    1085    1526    1548 
   Size:       1085     441      22

  Fragments arranged by size: 

                1085     441      22
 
BlpI GC'TnA_GC

Cuts at:      0     178    1548 
   Size:        178    1370
 
Bme1580I G_kGCm'C

Cuts at:      0     274     511    1169    1274    1548 
   Size:        274     237     658     105     274

  Fragments arranged by size: 

                 658     274     274     237     105
 
BmgBI CAC'GTC

Cuts at:      0    1218    1548 
   Size:       1218     330
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     763     976    1000    1390    1548 
   Size:         88     675     213      24     390     158

  Fragments arranged by size: 

                 675     390     213     158      88      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     470    1548 
   Size:        470    1078
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     990    1548 
   Size:        990     558
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1108    1548 
   Size:       1108     440
 
BsaAI yAC'GTr

Cuts at:      0     153     307    1146    1548 
   Size:        153     154     839     402

  Fragments arranged by size: 

                 839     402     154     153
 
BsaBI GATnn'nnATC

Cuts at:      0     688    1548 
   Size:        688     860
 
BsaHI Gr'CG_yC

Cuts at:      0     279    1502    1548 
   Size:        279    1223      46

  Fragments arranged by size: 

                1223     279      46
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1450    1548 
   Size:       1450      98
 
BseYI C'CCAG_C

Cuts at:      0      92     660     776     868    1252    1488    1548 
   Size:         92     568     116      92     384     236      60

  Fragments arranged by size: 

                 568     384     236     116      92      92      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     543    1171    1177    1548 
   Size:        181     362     628       6     371

  Fragments arranged by size: 

                 628     371     362     181       6
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     274    1016    1274    1298    1548 
   Size:         80     194     742     258      24     250

  Fragments arranged by size: 

                 742     258     250     194      80      24
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      80     274     404     511     869    1016    1169 
   Size:          8      72     194     130     107     358     147     153

Cuts at:   1169    1274    1298    1548 
   Size:        105      24     250

  Fragments arranged by size: 

                 358     250     194     153     147     130     107     105
                  72      24       8
 
BspHI T'CATG_A

Cuts at:      0     341     848    1548 
   Size:        341     507     700

  Fragments arranged by size: 

                 700     507     341
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     219    1548 
   Size:        219    1329
 
BsrBI CCG'CTC

Cuts at:      0     123    1548 
   Size:        123    1425
 
BssHII G'CGCG_C

Cuts at:      0      29     156    1548 
   Size:         29     127    1392

  Fragments arranged by size: 

                1392     127      29
 
BssSI C'ACGA_G

Cuts at:      0     199     769    1548 
   Size:        199     570     779

  Fragments arranged by size: 

                 779     570     199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     210    1158    1191    1548 
   Size:        210     948      33     357

  Fragments arranged by size: 

                 948     357     210      33
 
BstEII G'GTnAC_C

Cuts at:      0     408    1548 
   Size:        408    1140
 
BstYI r'GATC_y

Cuts at:      0     683    1085    1526    1548 
   Size:        683     402     441      22

  Fragments arranged by size: 

                 683     441     402      22
 
BtgI C'CryG_G

Cuts at:      0     413     904    1548 
   Size:        413     491     644

  Fragments arranged by size: 

                 644     491     413
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     615    1548 
   Size:        615     933
 
BtsI GCAGTG_nn'

Cuts at:      0     560    1548 
   Size:        560     988
 
DraIII CAC_nnn'GTG

Cuts at:      0     307     798    1146    1149    1548 
   Size:        307     491     348       3     399

  Fragments arranged by size: 

                 491     399     348     307       3
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1216    1548 
   Size:       1216     332
 
EaeI y'GGCC_r

Cuts at:      0      20     674    1548 
   Size:         20     654     874

  Fragments arranged by size: 

                 874     654      20
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     115    1548 
   Size:         43      72    1433

  Fragments arranged by size: 

                1433      72      43
 
EcoICRI GAG'CTC

Cuts at:      0    1014    1296    1548 
   Size:       1014     282     252

  Fragments arranged by size: 

                1014     282     252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79      88     763     955     976    1000    1117    1390 
   Size:         79       9     675     192      21      24     117     273

Cuts at:   1390    1548 
   Size:        158

  Fragments arranged by size: 

                 675     273     192     158     117      79      24      21
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     823    1183    1359    1548 
   Size:        823     360     176     189

  Fragments arranged by size: 

                 823     360     189     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0     738     951    1188    1352    1548 
   Size:        738     213     237     164     196

  Fragments arranged by size: 

                 738     237     213     196     164
 
EcoRV GAT'ATC

Cuts at:      0     141    1548 
   Size:        141    1407
 
FspI TGC'GCA

Cuts at:      0    1123    1548 
   Size:       1123     425
 
HaeII r_GCGC'y

Cuts at:      0      93     188     282    1548 
   Size:         93      95      94    1266

  Fragments arranged by size: 

                1266      95      94      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     511     543    1062    1094    1548 
   Size:        511      32     519      32     454

  Fragments arranged by size: 

                 519     511     454      32      32
 
KasI G'GCGC_C

Cuts at:      0     278    1548 
   Size:        278    1270
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     573    1548 
   Size:        573     975
 
MslI CAynn'nnrTG

Cuts at:      0    1162    1548 
   Size:       1162     386
 
MspA1I CmG'CkG

Cuts at:      0     679     780    1368    1548 
   Size:        679     101     588     180

  Fragments arranged by size: 

                 679     588     180     101
 
NarI GG'CG_CC

Cuts at:      0     279    1548 
   Size:        279    1269
 
NcoI C'CATG_G

Cuts at:      0     413    1548 
   Size:        413    1135
 
NdeI CA'TA_TG

Cuts at:      0    1317    1548 
   Size:       1317     231
 
NspI r_CATG'y

Cuts at:      0     922    1161    1548 
   Size:        922     239     387

  Fragments arranged by size: 

                 922     387     239
 
PasI CC'CwG_GG

Cuts at:      0    1107    1548 
   Size:       1107     441
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     449     798    1548 
   Size:        449     349     750

  Fragments arranged by size: 

                 750     449     349
 
PfoI T'CCnGG_A

Cuts at:      0    1016    1548 
   Size:       1016     532
 
PmlI CAC'GTG

Cuts at:      0    1146    1548 
   Size:       1146     402
 
PpuMI rG'GwC_Cy

Cuts at:      0    1352    1548 
   Size:       1352     196
 
PshAI GACnn'nnGTC

Cuts at:      0     844    1548 
   Size:        844     704
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     529     561    1548 
   Size:        529      32     987

  Fragments arranged by size: 

                 987     529      32
 
PstI C_TGCA'G

Cuts at:      0    1184    1548 
   Size:       1184     364
 
PvuII CAG'CTG

Cuts at:      0    1368    1548 
   Size:       1368     180
 
SacI G_AGCT'C

Cuts at:      0    1016    1298    1548 
   Size:       1016     282     250

  Fragments arranged by size: 

                1016     282     250
 
SapI GCTCTTCn'nnn_

Cuts at:      0      43    1548 
   Size:         43    1505
 
SfcI C'TryA_G

Cuts at:      0    1180    1548 
   Size:       1180     368
 
SfoI GGC'GCC

Cuts at:      0     280    1548 
   Size:        280    1268
 
SmlI C'TyrA_G

Cuts at:      0     969    1548 
   Size:        969     579
 
StuI AGG'CCT

Cuts at:      0    1265    1548 
   Size:       1265     283
 
StyI C'CwwG_G

Cuts at:      0     404     413    1548 
   Size:        404       9    1135

  Fragments arranged by size: 

                1135     404       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      39      84     153     781    1548 
   Size:         39      45      69     628     767

  Fragments arranged by size: 

                 767     628      69      45      39
 
TatI w'GTAC_w

Cuts at:      0     317     959    1548 
   Size:        317     642     589

  Fragments arranged by size: 

                 642     589     317
 
XmnI GAAnn'nnTTC

Cuts at:      0     793    1548 
   Size:        793     755
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AlfI    AlwNI    ApaLI
     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI   BssHII    BssSI   BstAPI   BstEII    BstYI
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII    Hin4I     KasI
     MmeI     MslI   MspA1I     NarI     NcoI     NdeI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsrI     PstI    PvuII
     SacI     SapI     SfcI     SfoI     SmlI     StuI     StyI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI    BciVI    BfrBI     BmrI     BmtI     BplI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrFI    BsrGI
    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI
     EciI    EcoRI     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NaeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI     PsiI   PspOMI
    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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