Restriction map for
pF1KB9855
(Linear) (Six-base) MAPSORT of: KIBB9855.seq Check: 5637 from: 1 to: 1170
KIBB9855.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 642 1170
Size: 642 528
Acc65I G'GTAC_C
Cuts at: 0 74 1170
Size: 74 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1170
Size: 531 639
AfeI AGC'GCT
Cuts at: 0 940 1170
Size: 940 230
AflIII A'CryG_T
Cuts at: 0 173 600 1170
Size: 173 427 570
Fragments arranged by size:
570 427 173
AleI CACnn'nnGTG
Cuts at: 0 187 1170
Size: 187 983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 1170
Size: 265 905
ApaI G_GGCC'C
Cuts at: 0 99 1100 1170
Size: 99 1001 70
Fragments arranged by size:
1001 99 70
AvaI C'yCGr_G
Cuts at: 0 25 676 841 1170
Size: 25 651 165 329
Fragments arranged by size:
651 329 165 25
BanI G'GyrC_C
Cuts at: 0 74 690 810 1170
Size: 74 616 120 360
Fragments arranged by size:
616 360 120 74
BanII G_rGCy'C
Cuts at: 0 71 99 326 470 1100 1170
Size: 71 28 227 144 630 70
Fragments arranged by size:
630 227 144 71 70 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 514 1170
Size: 514 656
BbvCI CC'TCA_GC
Cuts at: 0 959 1086 1170
Size: 959 127 84
Fragments arranged by size:
959 127 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 332 1170
Size: 298 34 838
Fragments arranged by size:
838 298 34
BclI T'GATC_A
Cuts at: 0 473 626 764 1170
Size: 473 153 138 406
Fragments arranged by size:
473 406 153 138
BglI GCCn_nnn'nGGC
Cuts at: 0 680 1170
Size: 680 490
BlpI GC'TnA_GC
Cuts at: 0 320 1170
Size: 320 850
Bme1580I G_kGCm'C
Cuts at: 0 84 99 316 427 695 950 1019 1100
Size: 84 15 217 111 268 255 69 81
Cuts at: 1100 1121 1170
Size: 21 49
Fragments arranged by size:
268 255 217 111 84 81 69 49
21 15
BmgBI CAC'GTC
Cuts at: 0 174 1170
Size: 174 996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1170
Size: 1139 31
Bpu10I CC'TnA_GC
Cuts at: 0 959 1086 1170
Size: 959 127 84
Fragments arranged by size:
959 127 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1170
Size: 181 989
BsaI GGTCTCn'nnnn_
Cuts at: 0 529 1170
Size: 529 641
BsaAI yAC'GTr
Cuts at: 0 36 601 1170
Size: 36 565 569
Fragments arranged by size:
569 565 36
BsaBI GATnn'nnATC
Cuts at: 0 445 1170
Size: 445 725
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 976 1170
Size: 433 543 194
Fragments arranged by size:
543 433 194
BseYI C'CCAG_C
Cuts at: 0 304 814 1170
Size: 304 510 356
Fragments arranged by size:
510 356 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 472 808 1014 1170
Size: 261 211 336 206 156
Fragments arranged by size:
336 261 211 206 156
BsiEI CG_ry'CG
Cuts at: 0 582 1170
Size: 582 588
Bsp1286I G_dGCh'C
Cuts at: 0 71 84 99 316 326 427 470 695
Size: 71 13 15 217 10 101 43 225
Cuts at: 695 950 1019 1100 1121 1170
Size: 255 69 81 21 49
Fragments arranged by size:
255 225 217 101 81 71 69 49
43 21 15 13 10
BspHI T'CATG_A
Cuts at: 0 470 503 1170
Size: 470 33 667
Fragments arranged by size:
667 470 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 830 1170
Size: 642 188 340
Fragments arranged by size:
642 340 188
BsrDI GCAATG_nn'
Cuts at: 0 211 1170
Size: 211 959
BstEII G'GTnAC_C
Cuts at: 0 292 607 1170
Size: 292 315 563
Fragments arranged by size:
563 315 292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 1170
Size: 189 981
BstYI r'GATC_y
Cuts at: 0 923 1170
Size: 923 247
BtgI C'CryG_G
Cuts at: 0 260 288 576 716 878 1092 1121 1170
Size: 260 28 288 140 162 214 29 49
Fragments arranged by size:
288 260 214 162 140 49 29 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 261 1170
Size: 261 909
BtsI GCAGTG_nn'
Cuts at: 0 314 1170
Size: 314 856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 194 229 1170
Size: 194 35 941
Fragments arranged by size:
941 194 35
DraIII CAC_nnn'GTG
Cuts at: 0 705 1170
Size: 705 465
EaeI y'GGCC_r
Cuts at: 0 202 431 579 914 1124 1170
Size: 202 229 148 335 210 46
Fragments arranged by size:
335 229 210 202 148 46
EagI C'GGCC_G
Cuts at: 0 579 1170
Size: 579 591
EarI CTCTTCn'nnn_
Cuts at: 0 259 1170
Size: 259 911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1139 1170
Size: 531 608 31
Fragments arranged by size:
608 531 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 700 1170
Size: 52 648 470
Fragments arranged by size:
648 470 52
EcoO109I rG'GnC_Cy
Cuts at: 0 57 95 837 1097 1170
Size: 57 38 742 260 73
Fragments arranged by size:
742 260 73 57 38
FspI TGC'GCA
Cuts at: 0 1105 1170
Size: 1105 65
HaeII r_GCGC'y
Cuts at: 0 638 942 1170
Size: 638 304 228
Fragments arranged by size:
638 304 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1170
Size: 1150 20
KpnI G_GTAC'C
Cuts at: 0 78 1170
Size: 78 1092
MscI TGG'CCA
Cuts at: 0 204 433 1126 1170
Size: 204 229 693 44
Fragments arranged by size:
693 229 204 44
MslI CAynn'nnrTG
Cuts at: 0 187 502 646 652 715 1012 1170
Size: 187 315 144 6 63 297 158
Fragments arranged by size:
315 297 187 158 144 63 6
MspA1I CmG'CkG
Cuts at: 0 90 245 762 1094 1170
Size: 90 155 517 332 76
Fragments arranged by size:
517 332 155 90 76
NcoI C'CATG_G
Cuts at: 0 260 716 1121 1170
Size: 260 456 405 49
Fragments arranged by size:
456 405 260 49
NspI r_CATG'y
Cuts at: 0 559 651 1170
Size: 559 92 519
Fragments arranged by size:
559 519 92
PflMI CCAn_nnn'nTGG
Cuts at: 0 266 705 1170
Size: 266 439 465
Fragments arranged by size:
465 439 266
PfoI T'CCnGG_A
Cuts at: 0 146 857 1170
Size: 146 711 313
Fragments arranged by size:
711 313 146
PpuMI rG'GwC_Cy
Cuts at: 0 57 837 1170
Size: 57 780 333
Fragments arranged by size:
780 333 57
PspOMI G'GGCC_C
Cuts at: 0 95 1096 1170
Size: 95 1001 74
Fragments arranged by size:
1001 95 74
PstI C_TGCA'G
Cuts at: 0 170 1170
Size: 170 1000
PvuII CAG'CTG
Cuts at: 0 245 1170
Size: 245 925
SacII CC_GC'GG
Cuts at: 0 1095 1170
Size: 1095 75
SapI GCTCTTCn'nnn_
Cuts at: 0 259 1170
Size: 259 911
SbfI CC_TGCA'GG
Cuts at: 0 170 1170
Size: 170 1000
SexAI A'CCwGG_T
Cuts at: 0 701 1170
Size: 701 469
SfcI C'TryA_G
Cuts at: 0 166 1170
Size: 166 1004
SmlI C'TyrA_G
Cuts at: 0 196 841 1170
Size: 196 645 329
Fragments arranged by size:
645 329 196
SphI G_CATG'C
Cuts at: 0 559 1170
Size: 559 611
StyI C'CwwG_G
Cuts at: 0 260 716 1121 1127 1170
Size: 260 456 405 6 43
Fragments arranged by size:
456 405 260 43 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 721 828 907 1170
Size: 595 126 107 79 263
Fragments arranged by size:
595 263 126 107 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 419 451 1170
Size: 184 32 203 32 719
Fragments arranged by size:
719 203 184 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 388 1170
Size: 388 782
XhoI C'TCGA_G
Cuts at: 0 841 1170
Size: 841 329
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI ApaI
AvaI BanI BanII BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BseRI BseYI BsgI BsiEI Bsp1286I BspHI BspMI
BsrDI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KpnI MscI MslI MspA1I NcoI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SapI
SbfI SexAI SfcI SmlI SphI StyI TaqII TstI
XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AloI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BglII BmrI BmtI BplI BsaHI
BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
ClaI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TatI Tth111I XbaI XmaI
XmnI ZraI