Restriction map for pF1KA0001
 (Linear) (Six-base) MAPSORT of: KIAA0001.seq  Check: 1913  from: 1  to: 1014



KIAA0001.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AflII C'TTAA_G

Cuts at:      0     652    1014 
   Size:        652     362
 
AflIII A'CryG_T

Cuts at:      0     269     311    1014 
   Size:        269      42     703

  Fragments arranged by size: 

                 703     269      42
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     879    1014 
   Size:        879     135
 
ApoI r'AATT_y

Cuts at:      0     666     832    1014 
   Size:        666     166     182

  Fragments arranged by size: 

                 666     182     166
 
BanII G_rGCy'C

Cuts at:      0     341    1014 
   Size:        341     673
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      87    1014 
   Size:         87     927
 
BclI T'GATC_A

Cuts at:      0       2      56    1014 
   Size:          2      54     958

  Fragments arranged by size: 

                 958      54       2
 
Bme1580I G_kGCm'C

Cuts at:      0     152    1014 
   Size:        152     862
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     544    1014 
   Size:        544     470
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11    1014 
   Size:         11    1003
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163    1014 
   Size:        163     851
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     291    1014 
   Size:        291     723
 
BsaAI yAC'GTr

Cuts at:      0     147    1014 
   Size:        147     867
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       9      39    1014 
   Size:          9      30     975

  Fragments arranged by size: 

                 975      30       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439    1014 
   Size:        439     575
 
BseYI C'CCAG_C

Cuts at:      0     151    1014 
   Size:        151     863
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300    1014 
   Size:        300     714
 
BsiHKAI G_wGCw'C

Cuts at:      0     299    1014 
   Size:        299     715
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     299     341    1014 
   Size:        152     147      42     673

  Fragments arranged by size: 

                 673     152     147      42
 
BsrDI GCAATG_nn'

Cuts at:      0      98    1014 
   Size:         98     916
 
BstYI r'GATC_y

Cuts at:      0     230    1014 
   Size:        230     784
 
EaeI y'GGCC_r

Cuts at:      0     575    1014 
   Size:        575     439
 
EarI CTCTTCn'nnn_

Cuts at:      0     304    1014 
   Size:        304     710
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11    1014 
   Size:         11    1003
 
EcoRI G'AATT_C

Cuts at:      0     666     832    1014 
   Size:        666     166     182

  Fragments arranged by size: 

                 666     182     166
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     654    1014 
   Size:        654     360
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     771     803    1014 
   Size:        771      32     211

  Fragments arranged by size: 

                 771     211      32
 
HincII GTy'rAC

Cuts at:      0     309    1014 
   Size:        309     705
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     643    1014 
   Size:        643     371
 
MscI TGG'CCA

Cuts at:      0     577    1014 
   Size:        577     437
 
MslI CAynn'nnrTG

Cuts at:      0      76     571    1014 
   Size:         76     495     443

  Fragments arranged by size: 

                 495     443      76
 
MspA1I CmG'CkG

Cuts at:      0     264     797    1014 
   Size:        264     533     217

  Fragments arranged by size: 

                 533     264     217
 
NspI r_CATG'y

Cuts at:      0     315    1014 
   Size:        315     699
 
PciI A'CATG_T

Cuts at:      0     311    1014 
   Size:        311     703
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     584    1014 
   Size:        584     430
 
PsiI TTA'TAA

Cuts at:      0     362    1014 
   Size:        362     652
 
PvuII CAG'CTG

Cuts at:      0     264     797    1014 
   Size:        264     533     217

  Fragments arranged by size: 

                 533     264     217
 
SapI GCTCTTCn'nnn_

Cuts at:      0     304    1014 
   Size:        304     710
 
SmlI C'TyrA_G

Cuts at:      0     178     652    1014 
   Size:        178     474     362

  Fragments arranged by size: 

                 474     362     178
 
SspI AAT'ATT

Cuts at:      0     468    1014 
   Size:        468     546
 
StuI AGG'CCT

Cuts at:      0     248    1014 
   Size:        248     766
 
StyI C'CwwG_G

Cuts at:      0     234     249    1014 
   Size:        234      15     765

  Fragments arranged by size: 

                 765     234      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     666     796    1014 
   Size:        666     130     218

  Fragments arranged by size: 

                 666     218     130
 
TatI w'GTAC_w

Cuts at:      0      83    1014 
   Size:         83     931
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       9      41    1014 
   Size:          9      32     973

  Fragments arranged by size: 

                 973      32       9
 
XmnI GAAnn'nnTTC

Cuts at:      0     832    1014 
   Size:        832     182
 

 Enzymes that do cut:

    AflII   AflIII     AlfI     ApoI    BanII     BbsI     BclI Bme1580I
     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BsrDI    BstYI     EaeI     EarI  Eco57MI
    EcoRI     FalI    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NspI     PciI    PflMI     PsiI    PvuII     SapI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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