################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BcgI BclI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI PstI PvuI PvuII RcaI RsrII SacII SbfI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1438 GTCGAC GT^[AC][GT]AC AclI 1332 AACGTT AA^CGTT AcyI 69 GGCGCC G[GA]^CG[CT]C Asp700I 496 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 581 GGTCC G^G[AT]CC BamHI 1446 GGATCC G^GATCC BanI 69 GGCGCC G^G[CT][GA]CC BbeI 69 GGCGCC GGCGC^C BbrPI 649 CACGTG CAC^GTG BciVI 1006 GTATCC GTATCC(6/5) BfaI 1556 CTAG C^TAG BlpI 685 GCTCAGC GC^T[ACGT]AGC Bpu1102I 685 GCTCAGC GC^T[ACGT]AGC BsaAI 649 CACGTG [CT]AC^GT[GA] BsaHI 69 GGCGCC G[GA]^CG[CT]C BsmAI 97 GTCTC GTCTC(1/5) BsmI 256 GAATGC GAATGC(1/-1) BspMI 117 ACCTGC ACCTGC(4/8) BsrFI 1572 GCCGGC [GA]^CCGG[CT] BstXI 472 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1446 GGATCC [GA]^GATC[CT] CelII 685 GCTCAGC GC^T[ACGT]AGC Cfr10I 1572 GCCGGC [GA]^CCGG[CT] EcoNI 1377 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 73 CCTTGG C^C[AT][AT]GG HaeII 69 GGCGCC [GA]GCGC^[CT] Hin1I 69 GGCGCC G[GA]^CG[CT]C HincII 1438 GTCGAC GT[CT]^[GA]AC HindII 1438 GTCGAC GT[CT]^[GA]AC KasI 69 GGCGCC G^GCGCC MaeI 1556 CTAG C^TAG MflI 1446 GGATCC [GA]^GATC[CT] NaeI 1572 GCCGGC GCC^GGC NarI 69 GGCGCC GG^CGCC NgoMIV 1572 GCCGGC G^CCGGC NotI 27 GCGGCCGC GC^GGCCGC PflMI 600 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 649 CACGTG CAC^GTG PmlI 649 CACGTG CAC^GTG PshAI 1431 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1332 AACGTT AA^CGTT SacI 1252 GAGCTC GAGCT^C SalI 1438 GTCGAC G^TCGAC ScaI 1340 AGTACT AGT^ACT SfcI 434 CTACAG C^T[GA][CT]AG SfoI 69 GGCGCC GGC^GCC StyI 73 CCTTGG C^C[AT][AT]GG Van91I 600 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1555 TCTAGA T^CTAGA XcmI 596 CCAACCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1446 GGATCC [GA]^GATC[CT] XmnI 496 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 545 ACGTGT A^C[GA][CT]GT 648 ACACGT AocI 1041 CCTGAGG CC^T[ACGT]AGG 1051 CCTCAGG ApaI 670 GGGCCC GGGCC^C 1323 GGGCCC BsgI 211 GTGCAG GTGCAG(16/14) 1645 GTGCAG BsiEI 12 CGGCCG CG[GA][CT]^CG 28 CGGCCG BstF5I 951 GGATG GGATG(2/0) 1527 GGATG Bsu36I 1041 CCTGAGG CC^T[ACGT]AGG 1051 CCTCAGG CfoI 70 GCGC GCG^C 1381 GCGC EaeI 12 CGGCCG [CT]^GGCC[GA] 28 CGGCCG EagI 12 CGGCCG C^GGCCG 28 CGGCCG EclXI 12 CGGCCG C^GGCCG 28 CGGCCG Eco52I 12 CGGCCG C^GGCCG 28 CGGCCG Eco81I 1041 CCTGAGG CC^T[ACGT]AGG 1051 CCTCAGG FokI 951 GGATG GGATG(9/13) 1527 GGATG HhaI 70 GCGC GCG^C 1381 GCGC HinP1I 70 GCGC G^CGC 1381 GCGC MslI 141 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 603 CACGCTGGTG SapI 1243 GAAGAGC GCTCTTC(1/4) 1417 GAAGAGC Tsp45I 592 GTGAC ^GT[GC]AC 1112 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI BanII BbsI BbvCI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I