(Circular) (Six-base) MAPSORT of: pFN21AE6755.seq Check: 9521 from: 1 to: 6364 pFN21AE6755 6364 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3444 3707 1937 Size: 1507 263 4594 Fragments arranged by size: 4594 1507 263 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 3338 3663 4491 4864 1563 Size: 1775 325 828 373 3063 Fragments arranged by size: 3063 1775 828 373 325 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 3144 3267 4427 5811 6079 1632 Size: 929 583 123 1160 1384 268 1917 Fragments arranged by size: 1917 1384 1160 929 583 268 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2550 2653 5263 6240 1203 Size: 1347 103 2610 977 1327 Fragments arranged by size: 2610 1347 1327 977 103 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 2612 1166 Size: 1446 4918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6364 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 2076 Size: 32 6332 AlwNI CAG_nnn'CTG Cuts at: 4107 5679 4107 Size: 1572 4792 ApaI G_GGCC'C Cuts at: 2679 3332 2679 Size: 653 5711 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 2863 2888 2967 3108 3673 3899 4051 6160 Size: 1370 25 79 141 565 226 152 2109 Cuts at: 6160 1493 Size: 1697 Fragments arranged by size: 2109 1697 1370 565 226 152 141 79 25 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 3689 1870 Size: 73 1746 4545 Fragments arranged by size: 4545 1746 73 BamHI G'GATC_C Cuts at: 3451 3694 4061 3451 Size: 243 367 5754 Fragments arranged by size: 5754 367 243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 3685 5140 618 Size: 325 217 627 287 1611 1455 1842 Fragments arranged by size: 1842 1611 1455 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 2014 2679 3261 3332 3540 3683 1391 Size: 623 665 582 71 208 143 4072 Fragments arranged by size: 4072 665 623 582 208 143 71 BbeI G_GCGC'C Cuts at: 2078 2078 Size: 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2180 2508 3545 928 Size: 971 281 328 1037 3747 Fragments arranged by size: 3747 1037 971 328 281 BbvCI CC'TCA_GC Cuts at: 3030 3167 3360 3030 Size: 137 193 6034 Fragments arranged by size: 6034 193 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4555 4589 1903 Size: 34 50 34 2534 34 3678 Fragments arranged by size: 3678 2534 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3022 4256 5466 3022 Size: 1234 1210 3920 Fragments arranged by size: 3920 1234 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4975 136 Size: 107 122 71 631 605 337 2966 1525 Fragments arranged by size: 2966 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 2691 3781 2691 Size: 1090 5274 Bme1580I G_kGCm'C Cuts at: 1790 2679 3332 4425 5581 1790 Size: 889 653 1093 1156 2573 Fragments arranged by size: 2573 1156 1093 889 653 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2584 3002 3457 Size: 416 53 83 399 24 1167 418 455 Cuts at: 3457 4095 5053 442 Size: 638 958 1753 Fragments arranged by size: 1753 1167 958 638 455 418 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3072 3072 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2460 2648 3567 5024 1239 Size: 1221 188 919 1457 2579 Fragments arranged by size: 2579 1457 1221 919 188 Bpu10I CC'TnA_GC Cuts at: 3030 3167 3360 3030 Size: 137 193 6034 Fragments arranged by size: 6034 193 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4489 5354 5652 4489 Size: 865 298 5201 Fragments arranged by size: 5201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5027 882 Size: 617 3528 2219 Fragments arranged by size: 3528 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2656 4155 493 Size: 711 1452 1499 2702 Fragments arranged by size: 2702 1499 1452 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2075 4171 4553 5188 Size: 53 83 186 936 542 2096 382 635 Cuts at: 5188 275 Size: 1451 Fragments arranged by size: 2096 1451 936 635 542 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4797 5195 5469 5616 6027 4797 Size: 398 274 147 411 5134 Fragments arranged by size: 5134 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2187 2217 3263 3293 1178 Size: 30 165 30 784 30 1046 30 4249 Fragments arranged by size: 4249 1046 784 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3083 3287 3422 3560 3587 1186 Size: 1897 204 135 138 27 3963 Fragments arranged by size: 3963 1897 204 138 135 27 BseYI C'CCAG_C Cuts at: 2857 3035 3153 5567 6063 2857 Size: 178 118 2414 496 3158 Fragments arranged by size: 3158 2414 496 178 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 3671 1163 Size: 573 501 1434 3856 Fragments arranged by size: 3856 1434 573 501 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 2036 3814 4575 5603 1540 Size: 275 186 19 16 1778 761 1028 2301 Fragments arranged by size: 2301 1778 1028 761 275 186 19 16 BsiHKAI G_wGCw'C Cuts at: 2619 2692 3261 3396 4425 4510 5581 2619 Size: 73 569 135 1029 85 1071 3402 Fragments arranged by size: 3402 1071 1029 569 135 85 73 BsmI GAATG_Cn' Cuts at: 2267 3875 3968 2267 Size: 1608 93 4663 Fragments arranged by size: 4663 1608 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2619 2679 2692 3261 3332 3396 Size: 399 224 605 60 13 569 71 64 Cuts at: 3396 3540 3683 4425 4510 5581 1391 Size: 144 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 605 569 399 224 144 143 85 71 64 60 13 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 4253 5983 4253 Size: 1730 4634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 3719 844 Size: 1287 1588 3489 Fragments arranged by size: 3489 1588 1287 BsrBI CCG'CTC Cuts at: 1394 1823 4251 1394 Size: 429 2428 3507 Fragments arranged by size: 3507 2428 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3577 5008 5195 1700 Size: 110 1767 1431 187 2869 Fragments arranged by size: 2869 1767 1431 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 4424 5436 4424 Size: 1012 5352 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 2484 2484 Size: 6364 BstYI r'GATC_y Cuts at: 1972 3451 3694 4061 4446 4463 5915 6001 6013 Size: 1479 243 367 385 17 1452 86 12 Cuts at: 6013 6359 1972 Size: 346 1977 Fragments arranged by size: 1977 1479 1452 385 367 346 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3047 3057 3047 Size: 10 6354 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5812 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5625 Fragments arranged by size: 5625 683 56 BtsI GCAGTG_nn' Cuts at: 2714 3010 3880 4674 4694 2714 Size: 296 870 794 20 4384 Fragments arranged by size: 4384 870 794 296 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5371 809 Size: 507 4055 1802 Fragments arranged by size: 4055 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2033 3811 Size: 54 1425 50 275 18 187 16 1778 Cuts at: 3811 4700 6329 8 Size: 889 1629 43 Fragments arranged by size: 1778 1629 1425 889 275 187 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2017 2033 3811 1537 Size: 275 205 16 1778 4090 Fragments arranged by size: 4090 1778 275 205 16 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 3060 3243 3417 4294 1353 Size: 1130 577 183 174 877 3423 Fragments arranged by size: 3423 1130 877 577 183 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4951 5325 5471 387 Size: 1064 138 3362 374 146 1280 Fragments arranged by size: 3362 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3259 3259 Size: 6364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2460 2561 2648 3144 3267 3567 4427 Size: 393 828 101 87 496 123 300 860 Cuts at: 4427 5024 5811 6079 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 860 828 787 597 496 393 300 268 123 101 87 EcoNI CCTnn'n_nnAGG Cuts at: 3386 3386 Size: 6364 EcoO109I rG'GnC_Cy Cuts at: 2675 2676 3051 3242 2675 Size: 1 375 191 5797 Fragments arranged by size: 5797 375 191 1 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 2078 5511 1678 Size: 400 3433 2531 Fragments arranged by size: 3433 2531 400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 2720 2752 3263 Size: 32 135 32 863 32 253 32 511 Cuts at: 3263 3295 5005 5037 5079 5111 1373 Size: 32 1710 32 42 32 2626 Fragments arranged by size: 2626 1710 863 511 253 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3445 3708 3954 669 Size: 988 281 1507 263 246 3079 Fragments arranged by size: 3079 1507 988 281 263 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 2074 2074 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5478 5662 1305 Size: 4173 184 2007 Fragments arranged by size: 4173 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4542 Fragments arranged by size: 4542 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2612 4322 4681 4840 518 Size: 648 984 462 1710 359 159 2042 Fragments arranged by size: 2042 1710 984 648 462 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2044 2053 3039 3780 4457 5605 5850 Size: 432 6 9 986 741 677 1148 245 Cuts at: 5850 1606 Size: 2120 Fragments arranged by size: 2120 1148 986 741 677 432 245 9 6 NaeI GCC'GGC Cuts at: 3579 3579 Size: 6364 NarI GG'CG_CC Cuts at: 2075 2075 Size: 6364 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5812 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NgoMIV G'CCGG_C Cuts at: 3577 3577 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 2033 3811 2033 Size: 1778 4586 NspI r_CATG'y Cuts at: 1132 1229 3722 5267 1132 Size: 97 2493 1545 2229 Fragments arranged by size: 2493 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2672 1348 Size: 1324 5040 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 2611 1108 Size: 1503 4861 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PmlI CAC'GTG Cuts at: 2656 2656 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3440 1613 Size: 1827 4537 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2675 3328 2675 Size: 653 5711 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2627 2659 2627 Size: 32 6332 PstI C_TGCA'G Cuts at: 830 3716 830 Size: 2886 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 SacI G_AGCT'C Cuts at: 3261 3261 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3443 3706 1936 Size: 1507 263 4594 Fragments arranged by size: 4594 1507 263 SapI GCTCTTCn'nnn_ Cuts at: 3243 3417 3243 Size: 174 6190 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 3347 4612 6324 3347 Size: 1265 1712 3387 Fragments arranged by size: 3387 1712 1265 SfcI C'TryA_G Cuts at: 826 1045 2439 3712 4847 5528 5719 826 Size: 219 1394 1273 1135 681 191 1471 Fragments arranged by size: 1471 1394 1273 1135 681 219 191 SfoI GGC'GCC Cuts at: 2076 2076 Size: 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4468 5369 5631 820 Size: 197 926 2525 901 262 1553 Fragments arranged by size: 2525 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2078 3803 513 Size: 552 1013 1725 3074 Fragments arranged by size: 3074 1725 1013 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2871 3167 4391 4408 4561 4746 5191 Size: 1255 1291 296 1224 17 153 185 445 Cuts at: 5191 325 Size: 1498 Fragments arranged by size: 1498 1291 1255 1224 445 296 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3345 4610 6322 Size: 274 80 33 51 1443 1368 1265 1712 Cuts at: 6322 96 Size: 138 Fragments arranged by size: 1712 1443 1368 1265 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 6324 6356 2645 Size: 32 3647 32 2653 Fragments arranged by size: 3647 2653 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3560 3700 3560 Size: 140 6224 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2608 2608 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 2505 4493 2505 Size: 1988 4376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI