################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BmyI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FseI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AfaI 6 GTAC GT^AC AflIII 251 ACATGT A^C[GA][CT]GT AlwI 1201 GATCC GGATC(4/5) Asp700I 610 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 77 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1015 GGACC G^G[AT]CC AviII 1112 TGCGCA TGC^GCA BbsI 1174 GAAGAC GAAGAC(2/6) BclI 1308 TGATCA T^GATCA BlpI 1452 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1452 GCTCAGC GC^T[ACGT]AGC BpuAI 1174 GAAGAC GAAGAC(2/6) BsaBI 529 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 1400 CCAAGG C^C[ACGT][ACGT]GG BseRI 899 GAGGAG GAGGAG(10/8) BsgI 788 GTGCAG GTGCAG(16/14) BsiYI 619 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 619 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspHI 1170 TCATGA T^CATGA BspLU11I 251 ACATGT A^CATGT BstXI 597 CCATAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1200 AGATCC [GA]^GATC[CT] CelII 1452 GCTCAGC GC^T[ACGT]AGC CfoI 1113 GCGC GCG^C DraII 166 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1130 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 619 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 166 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 1258 GAATTC G^AATTC EcoT14I 1400 CCAAGG C^C[AT][AT]GG FbaI 1308 TGATCA T^GATCA Fnu4HI 20 GCGGC GC^[ACGT]GC FspI 1112 TGCGCA TGC^GCA HaeIII 167 GGCC GG^CC HhaI 1113 GCGC GCG^C HinP1I 1113 GCGC G^CGC HindIII 557 AAGCTT A^AGCTT ItaI 20 GCGGC GC^[ACGT]GC MamI 529 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1200 AGATCC [GA]^GATC[CT] MwoI 1324 GCAAAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflFI 77 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 950 CTGCAG CTGCA^G RcaI 1170 TCATGA T^CATGA RsaI 6 GTAC GT^AC SexAI 1131 ACCTGGT A^CC[AT]GGT SfcI 950 CTGCAG C^T[GA][CT]AG SmlI 633 CTTGAG C^T[CT][GA]AG StyI 1400 CCAAGG C^C[AT][AT]GG Tth111I 77 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1200 AGATCC [GA]^GATC[CT] XmnI 610 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 353 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1265 CAGATTCTG BfaI 764 CTAG C^TAG 1334 CTAG BsmAI 629 GAGAC GTCTC(1/5) 946 GAGAC BssKI 619 CCTGG ^CC[ACGT]GG 1132 CCTGG BstNI 619 CCTGG CC^[AT]GG 1132 CCTGG Cac8I 474 GCTTGC GC[ACGT]^[ACGT]GC 1146 GCAAGC Cfr13I 167 GGCCC G^G[ACGT]CC 1015 GGACC EcoRII 619 CCTGG ^CC[AT]GG 1132 CCTGG MaeI 764 CTAG C^TAG 1334 CTAG MaeII 458 ACGT A^CGT 1379 ACGT MaeIII 455 GTTAC ^GT[ACGT]AC 1119 GTTAC MvaI 619 CCTGG CC^[AT]GG 1132 CCTGG NdeI 710 CATATG CA^TATG 941 CATATG NlaIV 167 GGCCCC GG[ACGT]^[ACGT]CC 1435 GGAACC NspI 251 ACATGT [GA]CATG^[CT] 1115 GCATGT Sau96I 167 GGCCC G^G[ACGT]CC 1015 GGACC ScrFI 619 CCTGG CC^[ACGT]GG 1132 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AcsI AluI ApoI BpmI BsrI BstF5I DdeI DpnII FokI HinfI HphI MboI MboII MnlI MseI MslI NdeII NlaIII PleI Sau3AI SfaNI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I