(Circular) (Six-base) MAPSORT of: pFN21AE2378.seq Check: 2279 from: 1 to: 6169 pFN21AE2378 6169 bp With 182 enzymes: SgfI * September 20, 2011 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4232 5616 5884 1632 Size: 2600 1384 268 1917 Fragments arranged by size: 2600 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 2256 5068 6045 1203 Size: 1053 2812 977 1327 Fragments arranged by size: 2812 1327 1053 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 2363 3275 3912 5484 2363 Size: 912 637 1572 3048 Fragments arranged by size: 3048 1572 912 637 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 2228 3112 3219 3263 3478 3704 3856 5965 Size: 735 884 107 44 215 226 152 2109 Cuts at: 5965 1493 Size: 1697 Fragments arranged by size: 2109 1697 884 735 226 215 152 107 44 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 3494 1870 Size: 73 1551 4545 Fragments arranged by size: 4545 1551 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2486 2519 2486 Size: 33 6136 BamHI G'GATC_C Cuts at: 3499 3866 3499 Size: 367 5802 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3490 4945 618 Size: 325 217 627 1703 1455 1842 Fragments arranged by size: 1842 1703 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3488 1391 Size: 2097 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3186 928 Size: 971 1287 3911 Fragments arranged by size: 3911 1287 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 3313 1548 Size: 1765 4404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4780 136 Size: 107 122 71 631 605 3108 1525 Fragments arranged by size: 3108 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 3458 3586 3458 Size: 128 6041 Bme1580I G_kGCm'C Cuts at: 1790 4230 5386 1790 Size: 2440 1156 2573 Fragments arranged by size: 2573 2440 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2828 3900 4858 Size: 416 53 83 399 24 1411 1072 958 Cuts at: 4858 442 Size: 1753 Fragments arranged by size: 1753 1411 1072 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2385 2436 3057 4829 1239 Size: 1146 51 621 1772 2579 Fragments arranged by size: 2579 1772 1146 621 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2659 4294 5159 5457 2659 Size: 1635 865 298 3371 Fragments arranged by size: 3371 1635 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3960 493 Size: 711 2756 2702 Fragments arranged by size: 2756 2702 711 BsaBI GATnn'nnATC Cuts at: 2538 6163 2538 Size: 3625 2544 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3976 4358 4993 275 Size: 53 83 186 936 2443 382 635 1451 Fragments arranged by size: 2443 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5944 Fragments arranged by size: 5944 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2919 1186 Size: 1733 4436 BseYI C'CCAG_C Cuts at: 5372 5868 5372 Size: 496 5673 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2814 1163 Size: 573 1078 4518 Fragments arranged by size: 4518 1078 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4230 4315 5386 4230 Size: 85 1071 5013 Fragments arranged by size: 5013 1071 85 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3488 4230 4315 5386 1391 Size: 399 1698 742 85 1071 2174 Fragments arranged by size: 2174 1698 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 3175 4058 5788 3175 Size: 883 1730 3556 Fragments arranged by size: 3556 1730 883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3524 844 Size: 2680 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4056 1394 Size: 429 2233 3507 Fragments arranged by size: 3507 2233 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4813 5000 1700 Size: 110 3003 187 2869 Fragments arranged by size: 3003 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 4229 5241 4229 Size: 1012 5157 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6169 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2609 2609 Size: 6169 BstYI r'GATC_y Cuts at: 1972 3205 3499 3866 4251 4268 5720 5806 5818 Size: 1233 294 367 385 17 1452 86 12 Cuts at: 5818 6164 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1233 385 367 346 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4678 Fragments arranged by size: 4678 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 2184 3685 4479 4499 2184 Size: 1501 794 20 3854 Fragments arranged by size: 3854 1501 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 3140 3140 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3616 4505 6134 Size: 54 1425 50 275 18 1786 889 1629 Cuts at: 6134 8 Size: 43 Fragments arranged by size: 1786 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4099 1353 Size: 2746 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4756 5130 5276 387 Size: 1064 138 3167 374 146 1280 Fragments arranged by size: 3167 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2385 2436 3057 4232 4829 5616 5884 Size: 393 753 51 621 1175 597 787 268 Cuts at: 5884 1239 Size: 1524 Fragments arranged by size: 1524 1175 787 753 621 597 393 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 2628 2628 Size: 6169 EcoO109I rG'GnC_Cy Cuts at: 2172 2172 Size: 6169 EcoRI G'AATT_C Cuts at: 3263 3478 3856 3263 Size: 215 378 5576 Fragments arranged by size: 5576 378 215 FspI TGC'GCA Cuts at: 3119 4675 3119 Size: 1556 4613 HaeII r_GCGC'y Cuts at: 1678 5316 1678 Size: 3638 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2191 2223 2243 2275 2696 Size: 32 135 32 619 32 20 32 421 Cuts at: 2696 2728 2789 2821 4810 4842 4884 4916 1373 Size: 32 61 32 1989 32 42 32 2626 Fragments arranged by size: 2626 1989 619 421 135 61 42 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 2562 748 Size: 1814 4355 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2275 2921 5283 5467 1305 Size: 970 646 2362 184 2007 Fragments arranged by size: 2362 2007 970 646 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4347 Fragments arranged by size: 4347 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2214 2544 2547 2607 4127 4486 4645 Size: 648 1048 330 3 60 1520 359 159 Cuts at: 4645 518 Size: 2042 Fragments arranged by size: 2042 1520 1048 648 359 330 159 60 3 MspA1I CmG'CkG Cuts at: 1606 3585 4262 5410 5655 1606 Size: 1979 677 1148 245 2120 Fragments arranged by size: 2120 1979 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4678 Fragments arranged by size: 4678 939 552 NdeI CA'TA_TG Cuts at: 387 2716 2947 387 Size: 2329 231 3609 Fragments arranged by size: 3609 2329 231 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 2260 3124 3527 5072 1132 Size: 97 1031 864 403 1545 2229 Fragments arranged by size: 2229 1545 1031 864 403 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 2256 5068 2256 Size: 2812 3357 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PstI C_TGCA'G Cuts at: 830 2959 3521 830 Size: 2129 562 3478 Fragments arranged by size: 3478 2129 562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SexAI A'CCwGG_T Cuts at: 3136 3136 Size: 6169 SfcI C'TryA_G Cuts at: 826 1045 2955 3517 4652 5333 5524 826 Size: 219 1910 562 1135 681 191 1471 Fragments arranged by size: 1910 1471 1135 681 562 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 3496 1872 Size: 1624 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2638 4273 5174 5436 820 Size: 197 926 695 1635 901 262 1553 Fragments arranged by size: 1635 1553 926 901 695 262 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 2004 3405 3608 513 Size: 552 939 1401 203 3074 Fragments arranged by size: 3074 1401 939 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4196 4213 4366 4551 4996 325 Size: 1255 2616 17 153 185 445 1498 Fragments arranged by size: 2616 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4415 6127 96 Size: 274 80 33 51 1443 2438 1712 138 Fragments arranged by size: 2438 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3126 3158 3238 3270 6129 6161 3126 Size: 32 80 32 2859 32 3134 Fragments arranged by size: 3134 2859 80 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2085 1316 Size: 769 5400 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 3494 1870 Size: 1624 4545 XmnI GAAnn'nnTTC Cuts at: 2619 4298 2619 Size: 1679 4490 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI