(Circular) (Six-base) MAPSORT of: pFN21AB9838.seq Check: 9616 from: 1 to: 5761 pFN21AB9838 5761 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2725 2909 3824 5208 5476 1632 Size: 1093 184 915 1384 268 1917 Fragments arranged by size: 1917 1384 1093 915 268 184 AfeI AGC'GCT Cuts at: 2123 2678 2123 Size: 555 5206 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2643 1166 Size: 1477 4284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2208 3504 5076 2208 Size: 1296 1572 2893 Fragments arranged by size: 2893 1572 1296 ApaI G_GGCC'C Cuts at: 2306 2306 Size: 5761 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2221 2285 2326 2387 2408 2509 Size: 73 168 110 64 41 61 21 101 Cuts at: 2509 2969 3086 1870 Size: 460 117 4545 Fragments arranged by size: 4545 460 168 117 110 101 73 64 61 41 21 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2225 2279 2309 2321 Size: 325 217 627 240 198 54 30 12 Cuts at: 2321 2546 2904 3082 4537 618 Size: 225 358 178 1455 1842 Fragments arranged by size: 1842 1455 627 358 325 240 225 217 198 178 54 30 12 BanII G_rGCy'C Cuts at: 1391 2306 3080 1391 Size: 915 774 4072 Fragments arranged by size: 4072 915 774 BbeI G_GCGC'C Cuts at: 2031 2283 2313 2550 2908 2031 Size: 252 30 237 358 4884 Fragments arranged by size: 4884 358 252 237 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 2495 2964 Size: 107 122 71 631 605 776 47 469 Cuts at: 2964 4372 136 Size: 1408 1525 Fragments arranged by size: 1525 1408 776 631 605 469 122 107 71 47 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2168 3178 2168 Size: 1010 4751 Bme1580I G_kGCm'C Cuts at: 1790 2193 2228 2306 2324 3044 3822 4978 1790 Size: 403 35 78 18 720 778 1156 2573 Fragments arranged by size: 2573 1156 778 720 403 78 35 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2690 2852 2867 2915 4421 1239 Size: 1124 327 162 15 48 1506 2579 Fragments arranged by size: 2579 1506 1124 327 162 48 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 2771 3886 4751 5049 2750 Size: 21 1115 865 298 3462 Fragments arranged by size: 3462 1115 865 298 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2662 3552 493 Size: 711 1458 890 2702 Fragments arranged by size: 2702 1458 890 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2162 2280 2310 Size: 53 83 186 936 495 134 118 30 Cuts at: 2310 2405 2547 2905 3568 3950 4585 275 Size: 95 142 358 663 382 635 1451 Fragments arranged by size: 1451 936 663 635 495 382 358 186 142 134 118 95 83 53 30 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2336 2366 1178 Size: 30 165 30 933 30 4573 Fragments arranged by size: 4573 933 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2173 2185 2687 2765 1186 Size: 987 12 502 78 4182 Fragments arranged by size: 4182 987 502 78 12 BseYI C'CCAG_C Cuts at: 2039 2201 2216 4964 5460 2039 Size: 162 15 2748 496 2340 Fragments arranged by size: 2748 2340 496 162 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2849 1163 Size: 573 1113 4075 Fragments arranged by size: 4075 1113 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2481 3048 3211 3972 5000 1540 Size: 275 186 480 567 163 761 1028 2301 Fragments arranged by size: 2301 1028 761 567 480 275 186 163 BsiHKAI G_wGCw'C Cuts at: 3822 3907 4978 3822 Size: 85 1071 4605 Fragments arranged by size: 4605 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 BsmBI CGTCTCn'nnnn_ Cuts at: 2271 2271 Size: 5761 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2228 2306 2324 3044 3080 3822 Size: 399 403 35 78 18 720 36 742 Cuts at: 3822 3907 4978 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 403 399 85 78 36 35 18 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2167 2298 2364 2534 2920 3648 1394 Size: 429 344 131 66 170 386 728 3507 Fragments arranged by size: 3507 728 429 386 344 170 131 66 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 2335 2549 2724 2987 3020 4405 Size: 110 418 107 214 175 263 33 1385 Cuts at: 4405 4592 1700 Size: 187 2869 Fragments arranged by size: 2869 1385 418 263 214 187 175 110 107 33 BsrGI T'GTAC_A Cuts at: 96 2928 96 Size: 2832 2929 BssHII G'CGCG_C Cuts at: 1925 2211 2451 2463 2565 1925 Size: 286 240 12 102 5121 Fragments arranged by size: 5121 286 240 102 12 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 2178 794 Size: 1384 4377 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstXI CCAn_nnnn'nTGG Cuts at: 2624 2624 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2347 3091 3458 3843 3860 5312 5398 5410 Size: 375 744 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 744 385 375 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2446 2458 2503 2560 2623 Size: 552 939 6 436 12 45 57 63 Cuts at: 2623 2734 2794 2996 513 Size: 111 60 202 3278 Fragments arranged by size: 3278 939 552 436 202 111 63 60 57 45 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2036 487 Size: 683 56 810 4212 Fragments arranged by size: 4212 810 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DraIII CAC_nnn'GTG Cuts at: 2662 2662 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2052 2440 Size: 54 1425 50 275 18 177 45 388 Cuts at: 2440 2478 2551 2910 3045 3208 4097 5726 8 Size: 38 73 359 135 163 889 1629 43 Fragments arranged by size: 1629 1425 889 388 359 275 177 163 135 73 54 50 45 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2478 3045 3208 1537 Size: 275 666 567 163 4090 Fragments arranged by size: 4090 666 567 275 163 EarI CTCTTCn'nnn_ Cuts at: 1353 3691 1353 Size: 2338 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2544 4348 4722 4868 387 Size: 1064 138 955 1804 374 146 1280 Fragments arranged by size: 1804 1280 1064 955 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2690 2725 2852 2867 2909 2915 Size: 393 731 327 35 127 15 42 6 Cuts at: 2915 3824 4421 5208 5476 1239 Size: 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 731 597 393 327 268 127 42 35 15 6 EcoNI CCTnn'n_nnAGG Cuts at: 2930 2930 Size: 5761 EcoO109I rG'GnC_Cy Cuts at: 2302 2303 2923 2302 Size: 1 620 5140 Fragments arranged by size: 5140 620 1 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2031 2125 2283 2313 2342 2396 2429 2550 Size: 353 94 158 30 29 54 33 121 Cuts at: 2550 2680 2908 4908 1678 Size: 130 228 2000 2531 Fragments arranged by size: 2531 2000 353 228 158 130 121 94 54 33 30 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373 Size: 32 135 32 2830 32 42 32 2626 Fragments arranged by size: 2830 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 3105 3351 669 Size: 988 281 130 1037 246 3079 Fragments arranged by size: 3079 1037 988 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KasI G'GCGC_C Cuts at: 2027 2279 2309 2546 2904 2027 Size: 252 30 237 358 4884 Fragments arranged by size: 4884 358 252 237 30 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2709 4875 5059 1305 Size: 1404 2166 184 2007 Fragments arranged by size: 2166 2007 1404 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2054 2912 10 Size: 54 1425 343 222 858 2859 Fragments arranged by size: 2859 1425 858 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 2622 2643 2708 3719 4078 4237 518 Size: 648 1456 21 65 1011 359 159 2042 Fragments arranged by size: 2042 1456 1011 648 359 159 65 21 MspA1I CmG'CkG Cuts at: 1606 2012 2050 2439 2505 2695 2736 2796 2836 Size: 406 38 389 66 190 41 60 40 Cuts at: 2836 2887 2998 3177 3854 5002 5247 1606 Size: 51 111 179 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 406 389 245 190 179 111 66 60 51 41 40 38 NaeI GCC'GGC Cuts at: 2337 2551 2337 Size: 214 5547 NarI GG'CG_CC Cuts at: 2028 2280 2310 2547 2905 2028 Size: 252 30 237 358 4884 Fragments arranged by size: 4884 358 252 237 30 NcoI C'CATG_G Cuts at: 513 1065 2004 2623 513 Size: 552 939 619 3651 Fragments arranged by size: 3651 939 619 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NgoMIV G'CCGG_C Cuts at: 2335 2549 2335 Size: 214 5547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 2478 3208 2478 Size: 730 5031 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PmlI CAC'GTG Cuts at: 2662 2662 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2923 2923 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PspOMI G'GGCC_C Cuts at: 2302 2302 Size: 5761 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2783 2815 2783 Size: 32 5729 PstI C_TGCA'G Cuts at: 830 3113 830 Size: 2283 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2695 2887 2695 Size: 192 5569 SacII CC_GC'GG Cuts at: 2013 2506 2737 2797 2999 2013 Size: 493 231 60 202 4775 Fragments arranged by size: 4775 493 231 202 60 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 3109 4244 4925 5116 826 Size: 219 2064 1135 681 191 1471 Fragments arranged by size: 2064 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2964 2964 Size: 5761 SfoI GGC'GCC Cuts at: 2029 2281 2311 2548 2906 2029 Size: 252 30 237 358 4884 Fragments arranged by size: 4884 358 252 237 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 2228 1700 Size: 528 5233 SmaI CCC'GGG Cuts at: 1872 2223 2287 2511 2971 3088 1872 Size: 351 64 224 460 117 4545 Fragments arranged by size: 4545 460 351 224 117 64 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 2786 3865 4766 5028 820 Size: 197 926 822 21 1079 901 262 1553 Fragments arranged by size: 1553 1079 926 901 822 262 197 21 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SrfI GCCC'GGGC Cuts at: 2223 2511 2223 Size: 288 5473 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 2623 3036 3200 513 Size: 552 939 619 413 164 3074 Fragments arranged by size: 3074 939 619 552 413 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 3805 3958 4143 4588 325 Size: 1255 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 2928 4007 Size: 274 80 33 51 1443 375 576 1079 Cuts at: 4007 5719 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1079 576 375 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2621 2047 Size: 574 5187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 2221 2285 2509 2969 3086 1870 Size: 351 64 224 460 117 4545 Fragments arranged by size: 4545 460 351 224 117 64 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI RsrII SacI SanDI SapI SexAI SwaI