(Circular) (Six-base) MAPSORT of: pFN21AE3481.seq Check: 4139 from: 1 to: 9694 pFN21AE3481 9694 bp With 182 enzymes: SgfI * March 5, 2012 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4525 4903 4525 Size: 378 9316 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3669 7504 278 Size: 53 83 186 936 2133 3835 2468 Fragments arranged by size: 3835 2468 2133 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4752 4989 6441 7037 1937 Size: 2815 237 1452 596 4594 Fragments arranged by size: 4594 2815 1452 596 237 Acc65I G'GTAC_C Cuts at: 2388 6177 7015 2388 Size: 3789 838 5067 Fragments arranged by size: 5067 3789 838 AclI AA'CG_TT Cuts at: 1563 4326 6993 7821 8194 1563 Size: 2763 2667 828 373 3063 Fragments arranged by size: 3063 2763 2667 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3773 3968 4811 5024 5164 5630 6027 6278 Size: 2141 195 843 213 140 466 397 251 Cuts at: 6278 6806 7757 9141 9409 1632 Size: 528 951 1384 268 1917 Fragments arranged by size: 2141 1917 1384 951 843 528 466 397 268 251 213 195 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9497 AflIII A'CryG_T Cuts at: 1203 2979 8593 9570 1203 Size: 1776 5614 977 1327 Fragments arranged by size: 5614 1776 1327 977 AgeI A'CCGG_T Cuts at: 8525 8525 Size: 9694 AhdI GACnn_n'nnGTC Cuts at: 2542 4927 8423 2542 Size: 2385 3496 3813 Fragments arranged by size: 3813 3496 2385 AleI CACnn'nnGTG Cuts at: 1166 6884 1166 Size: 5718 3976 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3290 1184 Size: 2106 7588 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4274 4306 4274 Size: 32 9662 AlwNI CAG_nnn'CTG Cuts at: 2171 3334 3544 5173 5232 5878 7437 9009 2171 Size: 1163 210 1629 59 646 1559 1572 2856 Fragments arranged by size: 2856 1629 1572 1559 1163 646 210 59 ApaI G_GGCC'C Cuts at: 3778 3778 Size: 9694 ApaLI G'TGCA_C Cuts at: 7751 8907 7751 Size: 1156 8538 ApoI r'AATT_y Cuts at: 1493 2726 4941 5630 6083 7003 7229 7381 9490 Size: 1233 2215 689 453 920 226 152 2109 Cuts at: 9490 1493 Size: 1697 Fragments arranged by size: 2215 2109 1697 1233 920 689 453 226 152 AseI AT'TA_AT Cuts at: 160 1032 8248 160 Size: 872 7216 1606 Fragments arranged by size: 7216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9694 AvaI C'yCGr_G Cuts at: 1870 1943 2893 2946 3404 3777 3889 5026 6899 Size: 73 950 53 458 373 112 1137 1873 Cuts at: 6899 7019 1870 Size: 120 4545 Fragments arranged by size: 4545 1873 1137 950 458 373 120 112 73 53 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5776 5809 5776 Size: 33 9661 BamHI G'GATC_C Cuts at: 2826 3039 4846 7024 7391 2826 Size: 213 1807 2178 367 5129 Fragments arranged by size: 5129 2178 1807 367 213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 3620 5221 6023 6177 Size: 325 217 627 601 1232 1601 802 154 Cuts at: 6177 6953 6984 7015 8470 618 Size: 776 31 31 1455 1842 Fragments arranged by size: 1842 1601 1455 1232 802 776 627 601 325 217 154 31 31 BanII G_rGCy'C Cuts at: 1391 3297 3399 3534 3689 3778 4584 5148 7013 Size: 1906 102 135 155 89 806 564 1865 Cuts at: 7013 1391 Size: 4072 Fragments arranged by size: 4072 1906 1865 806 564 155 135 102 89 BbeI G_GCGC'C Cuts at: 6957 6957 Size: 9694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2228 2538 2647 2961 3723 3910 4165 Size: 971 329 310 109 314 762 187 255 Cuts at: 4165 5251 5347 928 Size: 1086 96 5275 Fragments arranged by size: 5275 1086 971 762 329 314 310 255 187 109 96 BbvCI CC'TCA_GC Cuts at: 6546 6857 6546 Size: 311 9383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2299 2333 7885 7919 1903 Size: 34 362 34 5552 34 3678 Fragments arranged by size: 5552 3678 362 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5945 7586 8796 5945 Size: 1641 1210 6843 Fragments arranged by size: 6843 1641 1210 BclI T'GATC_A Cuts at: 1548 3483 1548 Size: 1935 7759 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3455 3974 4236 Size: 107 122 71 631 605 1783 519 262 Cuts at: 4236 5204 5435 5502 5717 8305 136 Size: 968 231 67 215 2588 1525 Fragments arranged by size: 2588 1783 1525 968 631 605 519 262 231 215 122 107 71 67 BglII A'GATC_T Cuts at: 3849 6165 9689 3849 Size: 2316 3524 3854 Fragments arranged by size: 3854 3524 2316 BlpI GC'TnA_GC Cuts at: 2909 7111 2909 Size: 4202 5492 Bme1580I G_kGCm'C Cuts at: 1790 3778 3992 5224 6989 7755 8911 1790 Size: 1988 214 1232 1765 766 1156 2573 Fragments arranged by size: 2573 1988 1765 1232 1156 766 214 BmgBI CAC'GTC Cuts at: 1318 3859 1318 Size: 2541 7153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2688 4955 5950 Size: 416 53 83 399 24 1271 2267 995 Cuts at: 5950 7425 8383 442 Size: 1475 958 1753 Fragments arranged by size: 2267 1753 1475 1271 995 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2908 4571 2908 Size: 1663 8031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2156 2777 3135 5351 6225 6447 6479 Size: 893 24 621 358 2216 874 222 32 Cuts at: 6479 6812 8354 1239 Size: 333 1542 2579 Fragments arranged by size: 2579 2216 1542 893 874 621 358 333 222 32 24 Bpu10I CC'TnA_GC Cuts at: 6054 6546 6857 6054 Size: 492 311 8891 Fragments arranged by size: 8891 492 311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2852 3283 4568 6077 6182 7819 8684 8982 2852 Size: 431 1285 1509 105 1637 865 298 3564 Fragments arranged by size: 3564 1637 1509 1285 865 431 298 105 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 3404 4928 5966 6180 6361 6827 Size: 617 618 1287 1524 1038 214 181 466 Cuts at: 6827 8357 882 Size: 1530 2219 Fragments arranged by size: 2219 1530 1524 1287 1038 618 617 466 214 181 BsaAI yAC'GTr Cuts at: 493 1204 2686 2980 3389 4407 5248 7485 493 Size: 711 1482 294 409 1018 841 2237 2702 Fragments arranged by size: 2702 2237 1482 1018 841 711 409 294 BsaBI GATnn'nnATC Cuts at: 3044 9688 3044 Size: 6644 3050 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2565 3517 3666 4012 Size: 53 83 186 936 1032 952 149 346 Cuts at: 4012 4645 5060 5859 6954 7501 7883 8518 275 Size: 633 415 799 1095 547 382 635 1451 Fragments arranged by size: 1451 1095 1032 952 936 799 635 633 547 415 382 346 186 149 83 53 BsaWI w'CCGG_w Cuts at: 2590 6115 6487 8127 8525 8799 8946 9357 2590 Size: 3525 372 1640 398 274 147 411 2927 Fragments arranged by size: 3525 2927 1640 411 398 372 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2279 2309 3204 3234 1178 Size: 30 165 30 876 30 895 30 7638 Fragments arranged by size: 7638 895 876 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 5495 6563 6809 6817 6917 1186 Size: 949 3360 1068 246 8 100 3963 Fragments arranged by size: 3963 3360 1068 949 246 100 8 BseYI C'CCAG_C Cuts at: 2024 2275 3248 5166 5189 5225 5661 5906 8897 Size: 251 973 1918 23 36 436 245 2991 Cuts at: 8897 9393 2024 Size: 496 2325 Fragments arranged by size: 2991 2325 1918 973 496 436 251 245 36 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 2957 3041 3215 3868 4043 4895 Size: 573 612 609 84 174 653 175 852 Cuts at: 4895 6752 6793 1163 Size: 1857 41 4064 Fragments arranged by size: 4064 1857 852 653 612 609 573 175 174 84 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7144 7905 8933 1540 Size: 275 186 5143 761 1028 2301 Fragments arranged by size: 5143 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3297 3399 4584 5148 6894 7755 7840 8911 3297 Size: 102 1185 564 1746 861 85 1071 4080 Fragments arranged by size: 4080 1746 1185 1071 861 564 102 85 BsmI GAATG_Cn' Cuts at: 2874 3111 6917 7205 7298 2874 Size: 237 3806 288 93 5270 Fragments arranged by size: 5270 3806 288 237 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3297 3399 3534 3689 3778 3992 4584 Size: 399 1507 102 135 155 89 214 592 Cuts at: 4584 5148 5224 6894 6989 7013 7755 7840 8911 Size: 564 76 1670 95 24 742 85 1071 Cuts at: 8911 1391 Size: 2174 Fragments arranged by size: 2174 1670 1507 1071 742 592 564 399 214 155 135 102 95 89 85 76 24 BspEI T'CCGG_A Cuts at: 2590 6487 9357 2590 Size: 3897 2870 2927 Fragments arranged by size: 3897 2927 2870 BspHI T'CATG_A Cuts at: 3486 4374 7583 9313 3486 Size: 888 3209 1730 3867 Fragments arranged by size: 3867 3209 1730 888 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2641 3378 4509 4525 4903 5476 6724 7049 Size: 1797 737 1131 16 378 573 1248 325 Cuts at: 7049 844 Size: 3489 Fragments arranged by size: 3489 1797 1248 1131 737 573 378 325 16 BsrBI CCG'CTC Cuts at: 1394 1823 2385 7581 1394 Size: 429 562 5196 3507 Fragments arranged by size: 5196 3507 562 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4269 5574 8189 8363 65 Size: 1183 3021 1305 2615 174 1396 Fragments arranged by size: 3021 2615 1396 1305 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3720 3911 4566 4718 8338 8525 1700 Size: 110 1910 191 655 152 3620 187 2869 Fragments arranged by size: 3620 2869 1910 655 191 187 152 110 BsrGI T'GTAC_A Cuts at: 96 6636 96 Size: 6540 3154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9694 BssSI C'ACGA_G Cuts at: 3452 3943 4120 7754 8766 3452 Size: 491 177 3634 1012 4380 Fragments arranged by size: 4380 3634 1012 491 177 BstAPI GCAn_nnn'nTGC Cuts at: 794 3334 4083 794 Size: 2540 749 6405 Fragments arranged by size: 6405 2540 749 BstBI TT'CG_AA Cuts at: 4525 8498 4525 Size: 3973 5721 BstEII G'GTnAC_C Cuts at: 1184 2761 3764 6097 6583 1184 Size: 1577 1003 2333 486 4295 Fragments arranged by size: 4295 2333 1577 1003 486 BstXI CCAn_nnnn'nTGG Cuts at: 2134 2251 3215 4234 4314 5824 2134 Size: 117 964 1019 80 1510 6004 Fragments arranged by size: 6004 1510 1019 964 117 80 BstYI r'GATC_y Cuts at: 1972 2448 2619 2826 3039 3066 3462 3849 4446 Size: 476 171 207 213 27 396 387 597 Cuts at: 4446 4846 5558 6165 7024 7391 7776 7793 9245 Size: 400 712 607 859 367 385 17 1452 Cuts at: 9245 9331 9343 9689 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 859 712 607 597 476 400 396 387 385 367 346 213 207 171 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2017 6135 2017 Size: 4118 5576 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2478 3168 3348 3769 3795 Size: 552 939 123 351 690 180 421 26 Cuts at: 3795 5064 513 Size: 1269 5143 Fragments arranged by size: 5143 1269 939 690 552 421 351 180 123 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5056 5266 487 Size: 683 56 3830 210 4915 Fragments arranged by size: 4915 3830 683 210 56 BtsI GCAGTG_nn' Cuts at: 3016 3901 3958 5108 7210 8004 8024 3016 Size: 885 57 1150 2102 794 20 4686 Fragments arranged by size: 4686 2102 1150 885 794 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9694 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2650 2685 529 Size: 35 2086 35 7538 Fragments arranged by size: 7538 2086 35 35 DraI TTT'AAA Cuts at: 6998 6998 Size: 9694 DraIII CAC_nnn'GTG Cuts at: 4407 4407 Size: 9694 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5297 6095 6712 8701 809 Size: 507 3981 798 617 1989 1802 Fragments arranged by size: 3981 1989 1802 798 617 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3222 3535 3628 Size: 54 1425 50 275 18 1392 313 93 Cuts at: 3628 3645 3706 3792 3955 4242 4629 5571 6267 Size: 17 61 86 163 287 387 942 696 Cuts at: 6267 6693 7141 8030 9659 8 Size: 426 448 889 1629 43 Fragments arranged by size: 1629 1425 1392 942 889 696 448 426 387 313 287 275 163 93 86 61 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 7141 1537 Size: 275 5329 4090 Fragments arranged by size: 5329 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 4272 5625 5964 6918 7624 1353 Size: 1046 1873 1353 339 954 706 3423 Fragments arranged by size: 3423 1873 1353 1046 954 706 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3581 8281 8655 8801 387 Size: 1064 138 1992 4700 374 146 1280 Fragments arranged by size: 4700 1992 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3295 3397 4582 5146 3295 Size: 102 1185 564 7843 Fragments arranged by size: 7843 1185 564 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2156 2777 3135 3773 3968 4811 Size: 393 500 24 621 358 638 195 843 Cuts at: 4811 5024 5164 5351 5630 6027 6225 6278 6447 Size: 213 140 187 279 397 198 53 169 Cuts at: 6447 6479 6806 6812 7757 8354 9141 9409 1239 Size: 32 327 6 945 597 787 268 1524 Fragments arranged by size: 1524 945 843 787 638 621 597 500 397 393 358 327 279 268 213 198 195 187 169 140 53 32 24 6 EcoNI CCTnn'n_nnAGG Cuts at: 4154 4259 6350 4154 Size: 105 2091 7498 Fragments arranged by size: 7498 2091 105 EcoO109I rG'GnC_Cy Cuts at: 2009 2021 2343 2752 3541 3774 4956 5136 5615 Size: 12 322 409 789 233 1182 180 479 Cuts at: 5615 5668 6946 2009 Size: 53 1278 4757 Fragments arranged by size: 4757 1278 1182 789 479 409 322 233 180 53 12 EcoRI G'AATT_C Cuts at: 4941 7003 7381 4941 Size: 2062 378 7254 Fragments arranged by size: 7254 2062 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4189 4189 Size: 9694 FspI TGC'GCA Cuts at: 3278 4076 8200 3278 Size: 798 4124 4772 Fragments arranged by size: 4772 4124 798 FspAI rTGC'GCAy Cuts at: 3278 3278 Size: 9694 HaeII r_GCGC'y Cuts at: 1678 4132 6957 8841 1678 Size: 2454 2825 1884 2531 Fragments arranged by size: 2825 2531 2454 1884 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 2528 2560 4913 Size: 32 135 32 707 32 217 32 2353 Cuts at: 4913 4945 5687 5719 6341 6373 8335 8367 8409 Size: 32 742 32 622 32 1962 32 42 Cuts at: 8409 8441 1373 Size: 32 2626 Fragments arranged by size: 2626 2353 1962 742 707 622 217 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2461 2500 2602 3424 4990 5275 Size: 988 281 523 39 102 822 1566 285 Cuts at: 5275 5491 6280 7038 7284 669 Size: 216 789 758 246 3079 Fragments arranged by size: 3079 1566 988 822 789 758 523 285 281 246 216 102 39 HindIII A'AGCT_T Cuts at: 748 748 Size: 9694 HpaI GTT'AAC Cuts at: 7284 7284 Size: 9694 KasI G'GCGC_C Cuts at: 6953 6953 Size: 9694 KpnI G_GTAC'C Cuts at: 2392 6181 7019 2392 Size: 3789 838 5067 Fragments arranged by size: 5067 3789 838 MfeI C'AATT_G Cuts at: 7293 7293 Size: 9694 MluI A'CGCG_T Cuts at: 9570 9570 Size: 9694 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3384 3948 4413 5923 6552 6718 8808 8992 Size: 2079 564 465 1510 629 166 2090 184 Cuts at: 8992 1305 Size: 2007 Fragments arranged by size: 2090 2079 2007 1510 629 564 465 184 166 MscI TGG'CCA Cuts at: 10 64 1489 1832 3224 3957 4244 4631 5573 Size: 54 1425 343 1392 733 287 387 942 Cuts at: 5573 6269 6695 10 Size: 696 426 3009 Fragments arranged by size: 3009 1425 1392 942 733 696 426 387 343 287 54 MslI CAynn'nnrTG Cuts at: 518 1166 3272 4478 5075 5822 6221 6884 7652 Size: 648 2106 1206 597 747 399 663 768 Cuts at: 7652 8011 8170 518 Size: 359 159 2042 Fragments arranged by size: 2106 2042 1206 768 747 663 648 597 399 359 159 MspA1I CmG'CkG Cuts at: 1606 3036 3627 5173 5661 6005 6265 6540 6664 Size: 1430 591 1546 488 344 260 275 124 Cuts at: 6664 7110 7787 8935 9180 1606 Size: 446 677 1148 245 2120 Fragments arranged by size: 2120 1546 1430 1148 677 591 488 446 344 275 260 245 124 NarI GG'CG_CC Cuts at: 6954 6954 Size: 9694 NcoI C'CATG_G Cuts at: 513 1065 2004 2127 513 Size: 552 939 123 8080 Fragments arranged by size: 8080 939 552 123 NdeI CA'TA_TG Cuts at: 387 4696 387 Size: 4309 5385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9694 NotI GC'GGCC_GC Cuts at: 7141 7141 Size: 9694 NspI r_CATG'y Cuts at: 1132 1229 4099 4432 7052 8597 1132 Size: 97 2870 333 2620 1545 2229 Fragments arranged by size: 2870 2620 2229 1545 333 97 PasI CC'CwG_GG Cuts at: 1348 3208 4150 1348 Size: 1860 942 6892 Fragments arranged by size: 6892 1860 942 PciI A'CATG_T Cuts at: 8593 8593 Size: 9694 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 3544 5173 5232 5839 1108 Size: 1245 1191 1629 59 607 4963 Fragments arranged by size: 4963 1629 1245 1191 607 59 PfoI T'CCnGG_A Cuts at: 2829 4176 4917 5328 5358 2829 Size: 1347 741 411 30 7165 Fragments arranged by size: 7165 1347 741 411 30 PmeI GTTT'AAAC Cuts at: 6998 6998 Size: 9694 PmlI CAC'GTG Cuts at: 4407 4407 Size: 9694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7763 7795 9276 9308 7763 Size: 32 1481 32 8149 Fragments arranged by size: 8149 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2009 2021 2343 3541 4956 5668 6946 2009 Size: 12 322 1198 1415 712 1278 4757 Fragments arranged by size: 4757 1415 1278 1198 712 322 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9694 PsiI TTA'TAA Cuts at: 7264 7264 Size: 9694 PspOMI G'GGCC_C Cuts at: 3774 3774 Size: 9694 PstI C_TGCA'G Cuts at: 830 2382 4625 4728 4900 5460 5730 7046 830 Size: 1552 2243 103 172 560 270 1316 3478 Fragments arranged by size: 3478 2243 1552 1316 560 270 172 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9694 PvuII CAG'CTG Cuts at: 5173 6005 6265 6664 5173 Size: 832 260 399 8203 Fragments arranged by size: 8203 832 399 260 SacI G_AGCT'C Cuts at: 3297 3399 4584 5148 3297 Size: 102 1185 564 7843 Fragments arranged by size: 7843 1185 564 102 SalI G'TCGA_C Cuts at: 1936 4988 7036 1936 Size: 3052 2048 4594 Fragments arranged by size: 4594 3052 2048 SanDI GG'GwC_CC Cuts at: 2009 2343 4956 2009 Size: 334 2613 6747 Fragments arranged by size: 6747 2613 334 SbfI CC_TGCA'GG Cuts at: 4728 7046 4728 Size: 2318 7376 ScaI AGT'ACT Cuts at: 3092 7942 9654 3092 Size: 4850 1712 3132 Fragments arranged by size: 4850 3132 1712 SexAI A'CCwGG_T Cuts at: 2958 3543 6173 2958 Size: 585 2630 6479 Fragments arranged by size: 6479 2630 585 SfcI C'TryA_G Cuts at: 826 1045 2378 2718 3340 4621 4724 4896 5456 Size: 219 1333 340 622 1281 103 172 560 Cuts at: 5456 5552 5726 7042 8177 8858 9049 826 Size: 96 174 1316 1135 681 191 1471 Fragments arranged by size: 1471 1333 1316 1281 1135 681 622 560 340 219 191 174 172 103 96 SfiI GGCCn_nnn'nGGCC Cuts at: 5435 5435 Size: 9694 SfoI GGC'GCC Cuts at: 6955 6955 Size: 9694 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9694 SgrAI Cr'CCGG_yG Cuts at: 1700 3911 1700 Size: 2211 7483 SmaI CCC'GGG Cuts at: 1872 3406 3779 3891 7021 1872 Size: 1534 373 112 3130 4545 Fragments arranged by size: 4545 3130 1534 373 112 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 3262 4583 6092 6161 7798 Size: 197 926 924 395 1321 1509 69 1637 Cuts at: 7798 8699 8961 820 Size: 901 262 1553 Fragments arranged by size: 1637 1553 1509 1321 926 924 901 395 262 197 69 SnaBI TAC'GTA Cuts at: 493 3389 7485 493 Size: 2896 4096 2702 Fragments arranged by size: 4096 2896 2702 SpeI A'CTAG_T Cuts at: 152 2203 152 Size: 2051 7643 SphI G_CATG'C Cuts at: 1132 4099 7052 1132 Size: 2967 2953 3774 Fragments arranged by size: 3774 2967 2953 SspI AAT'ATT Cuts at: 5 52 7618 5 Size: 47 7566 2081 Fragments arranged by size: 7566 2081 47 StuI AGG'CCT Cuts at: 1517 6133 6611 1517 Size: 4616 478 4600 Fragments arranged by size: 4616 4600 478 StyI C'CwwG_G Cuts at: 513 1065 2004 2127 4065 5139 5838 5976 6019 Size: 552 939 123 1938 1074 699 138 43 Cuts at: 6019 7133 513 Size: 1114 3074 Fragments arranged by size: 3074 1938 1114 1074 939 699 552 138 123 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3100 3649 5180 5239 5942 6874 6972 Size: 1255 1520 549 1531 59 703 932 98 Cuts at: 6972 7721 7738 7891 8076 8521 325 Size: 749 17 153 185 445 1498 Fragments arranged by size: 1531 1520 1498 1255 932 749 703 549 445 185 153 98 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 3090 6636 Size: 274 80 33 51 1443 102 1011 3546 Cuts at: 6636 6684 7940 9652 96 Size: 48 1256 1712 138 Fragments arranged by size: 3546 1712 1443 1256 1011 274 138 102 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2005 2037 3204 3236 3507 3539 4831 4863 9654 Size: 32 1167 32 271 32 1292 32 4791 Cuts at: 9654 9686 2005 Size: 32 2013 Fragments arranged by size: 4791 2013 1292 1167 271 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4049 4775 4913 5351 6353 6581 1316 Size: 2733 726 138 438 1002 228 4429 Fragments arranged by size: 4429 2733 1002 726 438 228 138 XbaI T'CTAG_A Cuts at: 7030 7030 Size: 9694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 4252 5390 6301 2248 Size: 2004 1138 911 5641 Fragments arranged by size: 5641 2004 1138 911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9694 XmaI C'CCGG_G Cuts at: 1870 3404 3777 3889 7019 1870 Size: 1534 373 112 3130 4545 Fragments arranged by size: 4545 3130 1534 373 112 XmnI GAAnn'nnTTC Cuts at: 4172 6557 7823 4172 Size: 2385 1266 6043 Fragments arranged by size: 6043 2385 1266 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3667 7502 276 Size: 53 83 186 936 2133 3835 2468 Fragments arranged by size: 3835 2468 2133 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FseI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SrfI SwaI