(Linear) (Six-base) MAPSORT of: KIEE3481.seq Check: 1971 from: 1 to: 4989 KIEE3481.orf With 182 enzymes: SgfI * March 5, 2012 15:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2520 2898 4989 Size: 2520 378 2091 Fragments arranged by size: 2520 2091 378 AatII G_ACGT'C Cuts at: 0 1664 4989 Size: 1664 3325 AccI GT'mk_AC Cuts at: 0 2747 2984 4436 4989 Size: 2747 237 1452 553 Fragments arranged by size: 2747 1452 553 237 Acc65I G'GTAC_C Cuts at: 0 383 4172 4989 Size: 383 3789 817 Fragments arranged by size: 3789 817 383 AclI AA'CG_TT Cuts at: 0 2321 4989 Size: 2321 2668 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1768 1963 2806 3019 3159 3625 4022 4273 Size: 1768 195 843 213 140 466 397 251 Cuts at: 4273 4801 4989 Size: 528 188 Fragments arranged by size: 1768 843 528 466 397 251 213 195 188 140 AflIII A'CryG_T Cuts at: 0 974 4989 Size: 974 4015 AhdI GACnn_n'nnGTC Cuts at: 0 537 2922 4989 Size: 537 2385 2067 Fragments arranged by size: 2385 2067 537 AleI CACnn'nnGTG Cuts at: 0 4879 4989 Size: 4879 110 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1285 4989 Size: 1285 3704 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2269 2301 4989 Size: 2269 32 2688 Fragments arranged by size: 2688 2269 32 AlwNI CAG_nnn'CTG Cuts at: 0 166 1329 1539 3168 3227 3873 4989 Size: 166 1163 210 1629 59 646 1116 Fragments arranged by size: 1629 1163 1116 646 210 166 59 ApaI G_GGCC'C Cuts at: 0 1773 4989 Size: 1773 3216 ApoI r'AATT_y Cuts at: 0 721 2936 3625 4078 4989 Size: 721 2215 689 453 911 Fragments arranged by size: 2215 911 721 689 453 AvaI C'yCGr_G Cuts at: 0 888 941 1399 1772 1884 3021 4894 4989 Size: 888 53 458 373 112 1137 1873 95 Fragments arranged by size: 1873 1137 888 458 373 112 95 53 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3771 3804 4989 Size: 3771 33 1185 Fragments arranged by size: 3771 1185 33 BamHI G'GATC_C Cuts at: 0 821 1034 2841 4989 Size: 821 213 1807 2148 Fragments arranged by size: 2148 1807 821 213 BanI G'GyrC_C Cuts at: 0 383 1615 3216 4018 4172 4948 4979 4989 Size: 383 1232 1601 802 154 776 31 10 Fragments arranged by size: 1601 1232 802 776 383 154 31 10 BanII G_rGCy'C Cuts at: 0 1292 1394 1529 1684 1773 2579 3143 4989 Size: 1292 102 135 155 89 806 564 1846 Fragments arranged by size: 1846 1292 806 564 155 135 102 89 BbeI G_GCGC'C Cuts at: 0 4952 4989 Size: 4952 37 BbsI GAAGACnn'nnnn_ Cuts at: 0 223 533 642 956 1718 1905 2160 3246 Size: 223 310 109 314 762 187 255 1086 Cuts at: 3246 3342 4989 Size: 96 1647 Fragments arranged by size: 1647 1086 762 314 310 255 223 187 109 96 BbvCI CC'TCA_GC Cuts at: 0 4541 4852 4989 Size: 4541 311 137 Fragments arranged by size: 4541 311 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 294 328 4989 Size: 294 34 4661 Fragments arranged by size: 4661 294 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3940 4989 Size: 3940 1049 BclI T'GATC_A Cuts at: 0 1478 4989 Size: 1478 3511 BglI GCCn_nnn'nGGC Cuts at: 0 1450 1969 2231 3199 3430 3497 3712 4989 Size: 1450 519 262 968 231 67 215 1277 Fragments arranged by size: 1450 1277 968 519 262 231 215 67 BglII A'GATC_T Cuts at: 0 1844 4160 4989 Size: 1844 2316 829 Fragments arranged by size: 2316 1844 829 BlpI GC'TnA_GC Cuts at: 0 904 4989 Size: 904 4085 Bme1580I G_kGCm'C Cuts at: 0 1773 1987 3219 4984 4989 Size: 1773 214 1232 1765 5 Fragments arranged by size: 1773 1765 1232 214 5 BmgBI CAC'GTC Cuts at: 0 1854 4989 Size: 1854 3135 BmrI ACTGGGnnnn_n' Cuts at: 0 683 2950 3945 4989 Size: 683 2267 995 1044 Fragments arranged by size: 2267 1044 995 683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 903 2566 4989 Size: 903 1663 2423 Fragments arranged by size: 2423 1663 903 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 151 772 1130 3346 4220 4442 4474 Size: 127 24 621 358 2216 874 222 32 Cuts at: 4474 4807 4989 Size: 333 182 Fragments arranged by size: 2216 874 621 358 333 222 182 127 32 24 Bpu10I CC'TnA_GC Cuts at: 0 4049 4541 4852 4989 Size: 4049 492 311 137 Fragments arranged by size: 4049 492 311 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 847 1278 2563 4072 4177 4989 Size: 847 431 1285 1509 105 812 Fragments arranged by size: 1509 1285 847 812 431 105 BsaI GGTCTCn'nnnn_ Cuts at: 0 112 1399 2923 3961 4175 4356 4822 4989 Size: 112 1287 1524 1038 214 181 466 167 Fragments arranged by size: 1524 1287 1038 466 214 181 167 112 BsaAI yAC'GTr Cuts at: 0 681 975 1384 2402 3243 4989 Size: 681 294 409 1018 841 1746 Fragments arranged by size: 1746 1018 841 681 409 294 BsaBI GATnn'nnATC Cuts at: 0 1039 4989 Size: 1039 3950 BsaHI Gr'CG_yC Cuts at: 0 560 1512 1661 2007 2640 3055 3854 4949 Size: 560 952 149 346 633 415 799 1095 Cuts at: 4949 4989 Size: 40 Fragments arranged by size: 1095 952 799 633 560 415 346 149 40 BsaWI w'CCGG_w Cuts at: 0 585 4110 4482 4989 Size: 585 3525 372 507 Fragments arranged by size: 3525 585 507 372 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 274 304 1199 1229 4989 Size: 274 30 895 30 3760 Fragments arranged by size: 3760 895 274 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 130 3490 4558 4804 4812 4912 4989 Size: 130 3360 1068 246 8 100 77 Fragments arranged by size: 3360 1068 246 130 100 77 8 BseYI C'CCAG_C Cuts at: 0 19 270 1243 3161 3184 3220 3656 3901 Size: 19 251 973 1918 23 36 436 245 Cuts at: 3901 4989 Size: 1088 Fragments arranged by size: 1918 1088 973 436 251 245 36 23 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 952 1036 1210 1863 2038 2890 4747 Size: 343 609 84 174 653 175 852 1857 Cuts at: 4747 4788 4989 Size: 41 201 Fragments arranged by size: 1857 852 653 609 343 201 175 174 84 41 BsiHKAI G_wGCw'C Cuts at: 0 1292 1394 2579 3143 4889 4989 Size: 1292 102 1185 564 1746 100 Fragments arranged by size: 1746 1292 1185 564 102 100 BsmI GAATG_Cn' Cuts at: 0 869 1106 4912 4989 Size: 869 237 3806 77 Fragments arranged by size: 3806 869 237 77 Bsp1286I G_dGCh'C Cuts at: 0 1292 1394 1529 1684 1773 1987 2579 3143 Size: 1292 102 135 155 89 214 592 564 Cuts at: 3143 3219 4889 4984 4989 Size: 76 1670 95 5 Fragments arranged by size: 1670 1292 592 564 214 155 135 102 95 89 76 5 BspEI T'CCGG_A Cuts at: 0 585 4482 4989 Size: 585 3897 507 Fragments arranged by size: 3897 585 507 BspHI T'CATG_A Cuts at: 0 1481 2369 4989 Size: 1481 888 2620 Fragments arranged by size: 2620 1481 888 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 636 1373 2504 2520 2898 3471 4719 4989 Size: 636 737 1131 16 378 573 1248 270 Fragments arranged by size: 1248 1131 737 636 573 378 270 16 BsrBI CCG'CTC Cuts at: 0 380 4989 Size: 380 4609 BsrDI GCAATG_nn' Cuts at: 0 2264 3569 4989 Size: 2264 1305 1420 Fragments arranged by size: 2264 1420 1305 BsrFI r'CCGG_y Cuts at: 0 1715 1906 2561 2713 4989 Size: 1715 191 655 152 2276 Fragments arranged by size: 2276 1715 655 191 152 BsrGI T'GTAC_A Cuts at: 0 4631 4989 Size: 4631 358 BssSI C'ACGA_G Cuts at: 0 1447 1938 2115 4989 Size: 1447 491 177 2874 Fragments arranged by size: 2874 1447 491 177 BstAPI GCAn_nnn'nTGC Cuts at: 0 1329 2078 4989 Size: 1329 749 2911 Fragments arranged by size: 2911 1329 749 BstBI TT'CG_AA Cuts at: 0 2520 4989 Size: 2520 2469 BstEII G'GTnAC_C Cuts at: 0 756 1759 4092 4578 4989 Size: 756 1003 2333 486 411 Fragments arranged by size: 2333 1003 756 486 411 BstXI CCAn_nnnn'nTGG Cuts at: 0 129 246 1210 2229 2309 3819 4989 Size: 129 117 964 1019 80 1510 1170 Fragments arranged by size: 1510 1170 1019 964 129 117 80 BstYI r'GATC_y Cuts at: 0 443 614 821 1034 1061 1457 1844 2441 Size: 443 171 207 213 27 396 387 597 Cuts at: 2441 2841 3553 4160 4989 Size: 400 712 607 829 Fragments arranged by size: 829 712 607 597 443 400 396 387 213 207 171 27 Bsu36I CC'TnA_GG Cuts at: 0 12 4130 4989 Size: 12 4118 859 Fragments arranged by size: 4118 859 12 BtgI C'CryG_G Cuts at: 0 122 473 1163 1343 1764 1790 3059 4989 Size: 122 351 690 180 421 26 1269 1930 Fragments arranged by size: 1930 1269 690 421 351 180 122 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3051 3261 4989 Size: 3051 210 1728 Fragments arranged by size: 3051 1728 210 BtsI GCAGTG_nn' Cuts at: 0 1011 1896 1953 3103 4989 Size: 1011 885 57 1150 1886 Fragments arranged by size: 1886 1150 1011 885 57 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 645 680 4989 Size: 645 35 4309 Fragments arranged by size: 4309 645 35 DraIII CAC_nnn'GTG Cuts at: 0 2402 4989 Size: 2402 2587 DrdI GACnn_nn'nnGTC Cuts at: 0 3292 4090 4707 4989 Size: 3292 798 617 282 Fragments arranged by size: 3292 798 617 282 EaeI y'GGCC_r Cuts at: 0 1217 1530 1623 1640 1701 1787 1950 2237 Size: 1217 313 93 17 61 86 163 287 Cuts at: 2237 2624 3566 4262 4688 4989 Size: 387 942 696 426 301 Fragments arranged by size: 1217 942 696 426 387 313 301 287 163 93 86 61 17 EarI CTCTTCn'nnn_ Cuts at: 0 394 2267 3620 3959 4913 4989 Size: 394 1873 1353 339 954 76 Fragments arranged by size: 1873 1353 954 394 339 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1576 4989 Size: 1576 3413 EcoICRI GAG'CTC Cuts at: 0 1290 1392 2577 3141 4989 Size: 1290 102 1185 564 1848 Fragments arranged by size: 1848 1290 1185 564 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 151 772 1130 1768 1963 2806 3019 Size: 127 24 621 358 638 195 843 213 Cuts at: 3019 3159 3346 3625 4022 4220 4273 4442 4474 Size: 140 187 279 397 198 53 169 32 Cuts at: 4474 4801 4807 4989 Size: 327 6 182 Fragments arranged by size: 843 638 621 397 358 327 279 213 198 195 187 182 169 140 127 53 32 24 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 2149 2254 4345 4989 Size: 2149 105 2091 644 Fragments arranged by size: 2149 2091 644 105 EcoO109I rG'GnC_Cy Cuts at: 0 4 16 338 747 1536 1769 2951 3131 Size: 4 12 322 409 789 233 1182 180 Cuts at: 3131 3610 3663 4941 4989 Size: 479 53 1278 48 Fragments arranged by size: 1278 1182 789 479 409 322 233 180 53 48 12 4 EcoRI G'AATT_C Cuts at: 0 2936 4989 Size: 2936 2053 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2184 4989 Size: 2184 2805 FspI TGC'GCA Cuts at: 0 1273 2071 4989 Size: 1273 798 2918 Fragments arranged by size: 2918 1273 798 FspAI rTGC'GCAy Cuts at: 0 1273 4989 Size: 1273 3716 HaeII r_GCGC'y Cuts at: 0 2127 4952 4989 Size: 2127 2825 37 Fragments arranged by size: 2825 2127 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 274 306 523 555 2908 2940 3682 3714 Size: 274 32 217 32 2353 32 742 32 Cuts at: 3714 4336 4368 4989 Size: 622 32 621 Fragments arranged by size: 2353 742 622 621 274 217 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 456 495 597 1419 2985 3270 3486 4275 Size: 456 39 102 822 1566 285 216 789 Cuts at: 4275 4989 Size: 714 Fragments arranged by size: 1566 822 789 714 456 285 216 102 39 KasI G'GCGC_C Cuts at: 0 4948 4989 Size: 4948 41 KpnI G_GTAC'C Cuts at: 0 387 4176 4989 Size: 387 3789 813 Fragments arranged by size: 3789 813 387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1379 1943 2408 3918 4547 4713 4989 Size: 1379 564 465 1510 629 166 276 Fragments arranged by size: 1510 1379 629 564 465 276 166 MscI TGG'CCA Cuts at: 0 1219 1952 2239 2626 3568 4264 4690 4989 Size: 1219 733 287 387 942 696 426 299 Fragments arranged by size: 1219 942 733 696 426 387 299 287 MslI CAynn'nnrTG Cuts at: 0 1267 2473 3070 3817 4216 4879 4989 Size: 1267 1206 597 747 399 663 110 Fragments arranged by size: 1267 1206 747 663 597 399 110 MspA1I CmG'CkG Cuts at: 0 1031 1622 3168 3656 4000 4260 4535 4659 Size: 1031 591 1546 488 344 260 275 124 Cuts at: 4659 4989 Size: 330 Fragments arranged by size: 1546 1031 591 488 344 330 275 260 124 NarI GG'CG_CC Cuts at: 0 4949 4989 Size: 4949 40 NcoI C'CATG_G Cuts at: 0 122 4989 Size: 122 4867 NdeI CA'TA_TG Cuts at: 0 2691 4989 Size: 2691 2298 NspI r_CATG'y Cuts at: 0 2094 2427 4989 Size: 2094 333 2562 Fragments arranged by size: 2562 2094 333 PasI CC'CwG_GG Cuts at: 0 1203 2145 4989 Size: 1203 942 2844 Fragments arranged by size: 2844 1203 942 PflMI CCAn_nnn'nTGG Cuts at: 0 348 1539 3168 3227 3834 4989 Size: 348 1191 1629 59 607 1155 Fragments arranged by size: 1629 1191 1155 607 348 59 PfoI T'CCnGG_A Cuts at: 0 824 2171 2912 3323 3353 4989 Size: 824 1347 741 411 30 1636 Fragments arranged by size: 1636 1347 824 741 411 30 PmlI CAC'GTG Cuts at: 0 2402 4989 Size: 2402 2587 PpuMI rG'GwC_Cy Cuts at: 0 4 16 338 1536 2951 3663 4941 4989 Size: 4 12 322 1198 1415 712 1278 48 Fragments arranged by size: 1415 1278 1198 712 322 48 12 4 PspOMI G'GGCC_C Cuts at: 0 1769 4989 Size: 1769 3220 PstI C_TGCA'G Cuts at: 0 377 2620 2723 2895 3455 3725 4989 Size: 377 2243 103 172 560 270 1264 Fragments arranged by size: 2243 1264 560 377 270 172 103 PvuII CAG'CTG Cuts at: 0 3168 4000 4260 4659 4989 Size: 3168 832 260 399 330 Fragments arranged by size: 3168 832 399 330 260 SacI G_AGCT'C Cuts at: 0 1292 1394 2579 3143 4989 Size: 1292 102 1185 564 1846 Fragments arranged by size: 1846 1292 1185 564 102 SalI G'TCGA_C Cuts at: 0 2983 4989 Size: 2983 2006 SanDI GG'GwC_CC Cuts at: 0 4 338 2951 4989 Size: 4 334 2613 2038 Fragments arranged by size: 2613 2038 334 4 SbfI CC_TGCA'GG Cuts at: 0 2723 4989 Size: 2723 2266 ScaI AGT'ACT Cuts at: 0 1087 4989 Size: 1087 3902 SexAI A'CCwGG_T Cuts at: 0 953 1538 4168 4989 Size: 953 585 2630 821 Fragments arranged by size: 2630 953 821 585 SfcI C'TryA_G Cuts at: 0 373 713 1335 2616 2719 2891 3451 3547 Size: 373 340 622 1281 103 172 560 96 Cuts at: 3547 3721 4989 Size: 174 1268 Fragments arranged by size: 1281 1268 622 560 373 340 174 172 103 96 SfiI GGCCn_nnn'nGGCC Cuts at: 0 3430 4989 Size: 3430 1559 SfoI GGC'GCC Cuts at: 0 4950 4989 Size: 4950 39 SgrAI Cr'CCGG_yG Cuts at: 0 1906 4989 Size: 1906 3083 SmaI CCC'GGG Cuts at: 0 1401 1774 1886 4989 Size: 1401 373 112 3103 Fragments arranged by size: 3103 1401 373 112 SmlI C'TyrA_G Cuts at: 0 862 1257 2578 4087 4156 4989 Size: 862 395 1321 1509 69 833 Fragments arranged by size: 1509 1321 862 833 395 69 SnaBI TAC'GTA Cuts at: 0 1384 4989 Size: 1384 3605 SpeI A'CTAG_T Cuts at: 0 198 4989 Size: 198 4791 SphI G_CATG'C Cuts at: 0 2094 4989 Size: 2094 2895 StuI AGG'CCT Cuts at: 0 4128 4606 4989 Size: 4128 478 383 Fragments arranged by size: 4128 478 383 StyI C'CwwG_G Cuts at: 0 122 2060 3134 3833 3971 4014 4989 Size: 122 1938 1074 699 138 43 975 Fragments arranged by size: 1938 1074 975 699 138 122 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1095 1644 3175 3234 3937 4869 4967 4989 Size: 1095 549 1531 59 703 932 98 22 Fragments arranged by size: 1531 1095 932 703 549 98 59 22 TatI w'GTAC_w Cuts at: 0 74 1085 4631 4679 4989 Size: 74 1011 3546 48 310 Fragments arranged by size: 3546 1011 310 74 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 32 1199 1231 1502 1534 2826 2858 4989 Size: 32 1167 32 271 32 1292 32 2131 Fragments arranged by size: 2131 1292 1167 271 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2044 2770 2908 3346 4348 4576 4989 Size: 2044 726 138 438 1002 228 413 Fragments arranged by size: 2044 1002 726 438 413 228 138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 243 2247 3385 4296 4989 Size: 243 2004 1138 911 693 Fragments arranged by size: 2004 1138 911 693 243 XmaI C'CCGG_G Cuts at: 0 1399 1772 1884 4989 Size: 1399 373 112 3105 Fragments arranged by size: 3105 1399 373 112 XmnI GAAnn'nnTTC Cuts at: 0 2167 4552 4989 Size: 2167 2385 437 Fragments arranged by size: 2385 2167 437 ZraI GAC'GTC Cuts at: 0 1662 4989 Size: 1662 3327 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AgeI ApaLI AscI AseI AsiSI AvrII BfrBI BmtI BsiEI BsiWI BsmBI BssHII BstZ17I ClaI DraI EagI EcoRV FseI HindIII HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SapI SgfI SrfI SspI SwaI XbaI XhoI