(Circular) (Six-base) MAPSORT of: pFN21AE2032.seq Check: 2595 from: 1 to: 6571 pFN21AE2032 6571 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3127 3565 3127 Size: 438 6133 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3914 1937 Size: 1977 4594 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 3546 4698 5071 1563 Size: 1983 1152 373 3063 Fragments arranged by size: 3063 1983 1152 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3409 3428 3545 3851 4634 6018 6286 1632 Size: 1777 19 117 306 783 1384 268 1917 Fragments arranged by size: 1917 1777 1384 783 306 268 117 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 3645 3817 5470 6447 1203 Size: 2442 172 1653 977 1327 Fragments arranged by size: 2442 1653 1327 977 172 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 3719 5300 3719 Size: 1581 4990 AleI CACnn'nnGTG Cuts at: 1166 3818 1166 Size: 2652 3919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AlwNI CAG_nnn'CTG Cuts at: 3169 4314 5886 3169 Size: 1145 1572 3854 Fragments arranged by size: 3854 1572 1145 ApaLI G'TGCA_C Cuts at: 2069 4628 5784 2069 Size: 2559 1156 2856 Fragments arranged by size: 2856 2559 1156 ApoI r'AATT_y Cuts at: 1493 2467 3880 4106 4258 6367 1493 Size: 974 1413 226 152 2109 1697 Fragments arranged by size: 2109 1697 1413 974 226 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2995 3896 1870 Size: 73 349 703 901 4545 Fragments arranged by size: 4545 901 703 349 73 BamHI G'GATC_C Cuts at: 3627 3901 4268 3627 Size: 274 367 5930 Fragments arranged by size: 5930 367 274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2673 3892 5347 618 Size: 325 217 627 886 1219 1455 1842 Fragments arranged by size: 1842 1455 1219 886 627 325 217 BanII G_rGCy'C Cuts at: 1391 2700 3069 3107 3734 3890 1391 Size: 1309 369 38 627 156 4072 Fragments arranged by size: 4072 1309 627 369 156 38 BbeI G_GCGC'C Cuts at: 2677 2677 Size: 6571 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5600 BbvCI CC'TCA_GC Cuts at: 2097 2331 2097 Size: 234 6337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3485 3834 4463 5673 3485 Size: 349 629 1210 4383 Fragments arranged by size: 4383 1210 629 349 BclI T'GATC_A Cuts at: 1548 3856 1548 Size: 2308 4263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2219 5182 136 Size: 107 122 71 631 605 547 2963 1525 Fragments arranged by size: 2963 1525 631 605 547 122 107 71 BglII A'GATC_T Cuts at: 3342 6566 3342 Size: 3224 3347 BlpI GC'TnA_GC Cuts at: 3988 3988 Size: 6571 Bme1580I G_kGCm'C Cuts at: 1790 2073 4632 5788 1790 Size: 283 2559 1156 2573 Fragments arranged by size: 2573 2559 1156 283 BmgBI CAC'GTC Cuts at: 1318 2014 2196 3534 1318 Size: 696 182 1338 4355 Fragments arranged by size: 4355 1338 696 182 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3558 4302 5260 Size: 416 53 83 399 24 2141 744 958 Cuts at: 5260 442 Size: 1753 Fragments arranged by size: 2141 1753 958 744 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2307 2307 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2192 2246 2593 3065 3467 5231 1239 Size: 863 90 54 347 472 402 1764 2579 Fragments arranged by size: 2579 1764 863 472 402 347 90 54 Bpu10I CC'TnA_GC Cuts at: 2097 2331 2738 2097 Size: 234 407 5930 Fragments arranged by size: 5930 407 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4696 5561 5859 4696 Size: 865 298 5408 Fragments arranged by size: 5408 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5234 882 Size: 617 3735 2219 Fragments arranged by size: 3735 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4362 493 Size: 711 3158 2702 Fragments arranged by size: 3158 2702 711 BsaBI GATnn'nnATC Cuts at: 3347 3632 6565 3347 Size: 285 2933 3353 Fragments arranged by size: 3353 2933 285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2288 2674 4378 4760 Size: 53 83 186 936 755 386 1704 382 Cuts at: 4760 5395 275 Size: 635 1451 Fragments arranged by size: 1704 1451 936 755 635 386 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3150 5004 5402 5676 5823 6234 3150 Size: 1854 398 274 147 411 3487 Fragments arranged by size: 3487 1854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2180 2195 2210 2682 Size: 30 165 30 762 15 15 15 472 Cuts at: 2682 2712 2804 2834 3158 3188 1178 Size: 30 92 30 324 30 4561 Fragments arranged by size: 4561 762 472 324 165 92 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 2165 2204 2222 2416 2548 2825 2894 Size: 899 80 39 18 194 132 277 69 Cuts at: 2894 2906 2909 1186 Size: 12 3 4848 Fragments arranged by size: 4848 899 277 194 132 80 69 39 18 12 3 BseYI C'CCAG_C Cuts at: 3201 3513 5774 6270 3201 Size: 312 2261 496 3502 Fragments arranged by size: 3502 2261 496 312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 2789 1163 Size: 573 482 571 4945 Fragments arranged by size: 4945 573 571 482 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2111 3605 4021 4782 5810 1540 Size: 275 186 110 1494 416 761 1028 2301 Fragments arranged by size: 2301 1494 1028 761 416 275 186 110 BsiHKAI G_wGCw'C Cuts at: 2073 3069 4632 4717 5788 2073 Size: 996 1563 85 1071 2856 Fragments arranged by size: 2856 1563 1071 996 85 BsmI GAATG_Cn' Cuts at: 2549 4082 4175 2549 Size: 1533 93 4945 Fragments arranged by size: 4945 1533 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 3458 3524 3772 2186 Size: 1272 66 248 4985 Fragments arranged by size: 4985 1272 248 66 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2700 3069 3107 3734 3890 4632 Size: 399 283 627 369 38 627 156 742 Cuts at: 4632 4717 5788 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 627 627 399 369 283 156 85 38 BspEI T'CCGG_A Cuts at: 3150 6234 3150 Size: 3084 3487 BspHI T'CATG_A Cuts at: 3723 4460 6190 3723 Size: 737 1730 4104 Fragments arranged by size: 4104 1730 737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3127 3565 3594 3926 844 Size: 2283 438 29 332 3489 Fragments arranged by size: 3489 2283 438 332 29 BsrBI CCG'CTC Cuts at: 1394 1823 3601 4458 1394 Size: 429 1778 857 3507 Fragments arranged by size: 3507 1778 857 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3390 5066 5240 65 Size: 1183 2142 1676 174 1396 Fragments arranged by size: 2142 1676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3604 5215 5402 1700 Size: 110 1794 1611 187 2869 Fragments arranged by size: 2869 1794 1611 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6571 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 2388 3142 794 Size: 1594 754 4223 Fragments arranged by size: 4223 1594 754 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 2683 1184 Size: 1499 5072 BstYI r'GATC_y Cuts at: 1972 3342 3627 3901 4268 4653 4670 6122 6208 Size: 1370 285 274 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1370 385 367 346 285 274 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2235 2496 2886 513 Size: 552 939 231 261 390 4198 Fragments arranged by size: 4198 939 552 390 261 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2600 487 Size: 683 56 1374 4458 Fragments arranged by size: 4458 1374 683 56 BtsI GCAGTG_nn' Cuts at: 2645 3220 4087 4881 4901 2645 Size: 575 867 794 20 4315 Fragments arranged by size: 4315 867 794 575 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DraIII CAC_nnn'GTG Cuts at: 3445 3445 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3203 5578 809 Size: 507 1887 2375 1802 Fragments arranged by size: 2375 1887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2563 2832 3602 Size: 54 1425 50 275 18 733 269 770 Cuts at: 3602 4018 4907 6536 8 Size: 416 889 1629 43 Fragments arranged by size: 1629 1425 889 770 733 416 275 269 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3602 4018 1537 Size: 275 1790 416 4090 Fragments arranged by size: 4090 1790 416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3149 3722 4501 1353 Size: 1796 573 779 3423 Fragments arranged by size: 3423 1796 779 573 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3815 5158 5532 5678 387 Size: 1064 138 2226 1343 374 146 1280 Fragments arranged by size: 2226 1343 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3067 3067 Size: 6571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2192 2246 2593 3065 3409 3428 Size: 393 470 90 54 347 472 344 19 Cuts at: 3428 3467 3545 3851 4634 5231 6018 6286 1239 Size: 39 78 306 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 472 470 393 347 344 306 268 90 78 54 39 19 EcoNI CCTnn'n_nnAGG Cuts at: 3672 3672 Size: 6571 EcoO109I rG'GnC_Cy Cuts at: 2501 2734 2985 3205 3713 2501 Size: 233 251 220 508 5359 Fragments arranged by size: 5359 508 251 233 220 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 EcoRV GAT'ATC Cuts at: 3327 3327 Size: 6571 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3751 3751 Size: 6571 FspI TGC'GCA Cuts at: 2159 3278 5077 2159 Size: 1119 1799 3653 Fragments arranged by size: 3653 1799 1119 FspAI rTGC'GCAy Cuts at: 3278 3278 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 2677 5718 1678 Size: 999 3041 2531 Fragments arranged by size: 3041 2531 999 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2744 2776 2906 2938 3023 Size: 32 135 32 1172 32 130 32 85 Cuts at: 3023 3055 3705 3737 5212 5244 5286 5318 1373 Size: 32 650 32 1475 32 42 32 2626 Fragments arranged by size: 2626 1475 1172 650 135 130 85 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3915 4161 669 Size: 988 281 1977 246 3079 Fragments arranged by size: 3079 1977 988 281 246 HindIII A'AGCT_T Cuts at: 748 2300 748 Size: 1552 5019 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KasI G'GCGC_C Cuts at: 2673 2673 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3315 5685 5869 1305 Size: 2010 2370 184 2007 Fragments arranged by size: 2370 2010 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2565 10 Size: 54 1425 343 733 4016 Fragments arranged by size: 4016 1425 733 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3818 4529 4888 5047 518 Size: 648 2652 711 359 159 2042 Fragments arranged by size: 2652 2042 711 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2122 2765 2773 3201 3747 3987 4664 Size: 423 93 643 8 428 546 240 677 Cuts at: 4664 5812 6057 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 643 546 428 423 245 240 93 8 NarI GG'CG_CC Cuts at: 2674 2674 Size: 6571 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5080 Fragments arranged by size: 5080 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 2084 2313 2363 3127 3649 3821 3929 Size: 97 855 229 50 764 522 172 108 Cuts at: 3929 5474 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 855 764 522 229 172 108 97 50 PasI CC'CwG_GG Cuts at: 1348 3240 1348 Size: 1892 4679 PciI A'CATG_T Cuts at: 3645 3817 5470 3645 Size: 172 1653 4746 Fragments arranged by size: 4746 1653 172 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6571 PfoI T'CCnGG_A Cuts at: 3069 3069 Size: 6571 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2501 2985 3205 3713 2501 Size: 484 220 508 5359 Fragments arranged by size: 5359 508 484 220 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PstI C_TGCA'G Cuts at: 830 3576 3923 830 Size: 2746 347 3478 Fragments arranged by size: 3478 2746 347 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2029 2765 2773 2029 Size: 736 8 5827 Fragments arranged by size: 5827 736 8 SacI G_AGCT'C Cuts at: 3069 3069 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SanDI GG'GwC_CC Cuts at: 2501 3713 2501 Size: 1212 5359 SapI GCTCTTCn'nnn_ Cuts at: 3722 3722 Size: 6571 SbfI CC_TGCA'GG Cuts at: 3576 3923 3576 Size: 347 6224 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SexAI A'CCwGG_T Cuts at: 3299 3299 Size: 6571 SfcI C'TryA_G Cuts at: 826 1045 2459 2517 2580 3572 3919 5054 5735 Size: 219 1414 58 63 992 347 1135 681 Cuts at: 5735 5926 826 Size: 191 1471 Fragments arranged by size: 1471 1414 1135 992 681 347 219 191 63 58 SfoI GGC'GCC Cuts at: 2675 2675 Size: 6571 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 3898 1872 Size: 2026 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2292 4675 5576 5838 820 Size: 197 926 349 2383 901 262 1553 Fragments arranged by size: 2383 1553 926 901 349 262 197 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 2213 1517 Size: 696 5875 StyI C'CwwG_G Cuts at: 513 1065 2004 2730 3131 4010 513 Size: 552 939 726 401 879 3074 Fragments arranged by size: 3074 939 879 726 552 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4598 4615 4768 4953 5398 325 Size: 1255 3018 17 153 185 445 1498 Fragments arranged by size: 3018 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 4817 6529 Size: 274 80 33 51 1443 285 2555 1712 Cuts at: 6529 96 Size: 138 Fragments arranged by size: 2555 1712 1443 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2165 2180 2197 2212 2428 2460 2682 2714 2804 Size: 15 17 15 216 32 222 32 90 Cuts at: 2804 2836 3158 3190 3807 3839 6531 6563 2165 Size: 32 322 32 617 32 2692 32 2173 Fragments arranged by size: 2692 2173 617 322 222 216 90 32 32 32 32 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 2143 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 2292 1943 Size: 349 6222 XmaI C'CCGG_G Cuts at: 1870 3896 1870 Size: 2026 4545 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SrfI SwaI