Restriction map for
pFN21AE2032
(Linear) (Six-base) MAPSORT of: KIEE2032.seq Check: 2472 from: 1 to: 1866
KIEE2032.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1122 1560 1866
Size: 1122 438 306
Fragments arranged by size:
1122 438 306
AclI AA'CG_TT
Cuts at: 0 1541 1866
Size: 1541 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1404 1423 1540 1846 1866
Size: 1404 19 117 306 20
Fragments arranged by size:
1404 306 117 20 19
AflIII A'CryG_T
Cuts at: 0 1640 1812 1866
Size: 1640 172 54
Fragments arranged by size:
1640 172 54
AhdI GACnn_n'nnGTC
Cuts at: 0 1714 1866
Size: 1714 152
AleI CACnn'nnGTG
Cuts at: 0 1813 1866
Size: 1813 53
AlwNI CAG_nnn'CTG
Cuts at: 0 1164 1866
Size: 1164 702
ApaLI G'TGCA_C
Cuts at: 0 64 1866
Size: 64 1802
ApoI r'AATT_y
Cuts at: 0 462 1866
Size: 462 1404
AvaI C'yCGr_G
Cuts at: 0 287 990 1866
Size: 287 703 876
Fragments arranged by size:
876 703 287
BamHI G'GATC_C
Cuts at: 0 1622 1866
Size: 1622 244
BanI G'GyrC_C
Cuts at: 0 668 1866
Size: 668 1198
BanII G_rGCy'C
Cuts at: 0 695 1064 1102 1729 1866
Size: 695 369 38 627 137
Fragments arranged by size:
695 627 369 137 38
BbeI G_GCGC'C
Cuts at: 0 672 1866
Size: 672 1194
BbvCI CC'TCA_GC
Cuts at: 0 92 326 1866
Size: 92 234 1540
Fragments arranged by size:
1540 234 92
BciVI GTATCCnnnnn_n'
Cuts at: 0 1480 1829 1866
Size: 1480 349 37
Fragments arranged by size:
1480 349 37
BclI T'GATC_A
Cuts at: 0 1851 1866
Size: 1851 15
BglI GCCn_nnn'nGGC
Cuts at: 0 214 1866
Size: 214 1652
BglII A'GATC_T
Cuts at: 0 1337 1866
Size: 1337 529
Bme1580I G_kGCm'C
Cuts at: 0 68 1866
Size: 68 1798
BmgBI CAC'GTC
Cuts at: 0 9 191 1529 1866
Size: 9 182 1338 337
Fragments arranged by size:
1338 337 182 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1553 1866
Size: 1553 313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 302 1866
Size: 302 1564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 187 241 588 1060 1462 1866
Size: 97 90 54 347 472 402 404
Fragments arranged by size:
472 404 402 347 97 90 54
Bpu10I CC'TnA_GC
Cuts at: 0 92 326 733 1866
Size: 92 234 407 1133
Fragments arranged by size:
1133 407 234 92
BsaBI GATnn'nnATC
Cuts at: 0 1342 1627 1866
Size: 1342 285 239
Fragments arranged by size:
1342 285 239
BsaHI Gr'CG_yC
Cuts at: 0 283 669 1866
Size: 283 386 1197
Fragments arranged by size:
1197 386 283
BsaWI w'CCGG_w
Cuts at: 0 1145 1866
Size: 1145 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 175 190 205 677 707 799 829
Size: 160 15 15 15 472 30 92 30
Cuts at: 829 1153 1183 1866
Size: 324 30 683
Fragments arranged by size:
683 472 324 160 92 30 30 30
15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 160 199 217 411 543 820 889
Size: 80 80 39 18 194 132 277 69
Cuts at: 889 901 904 1866
Size: 12 3 962
Fragments arranged by size:
962 277 194 132 80 80 69 39
18 12 3
BseYI C'CCAG_C
Cuts at: 0 1196 1508 1866
Size: 1196 312 358
Fragments arranged by size:
1196 358 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 784 1866
Size: 213 571 1082
Fragments arranged by size:
1082 571 213
BsiEI CG_ry'CG
Cuts at: 0 106 1600 1866
Size: 106 1494 266
Fragments arranged by size:
1494 266 106
BsiHKAI G_wGCw'C
Cuts at: 0 68 1064 1866
Size: 68 996 802
Fragments arranged by size:
996 802 68
BsmI GAATG_Cn'
Cuts at: 0 544 1866
Size: 544 1322
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 1453 1519 1767 1866
Size: 181 1272 66 248 99
Fragments arranged by size:
1272 248 181 99 66
Bsp1286I G_dGCh'C
Cuts at: 0 68 695 1064 1102 1729 1866
Size: 68 627 369 38 627 137
Fragments arranged by size:
627 627 369 137 68 38
BspEI T'CCGG_A
Cuts at: 0 1145 1866
Size: 1145 721
BspHI T'CATG_A
Cuts at: 0 1718 1866
Size: 1718 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1122 1560 1589 1866
Size: 1122 438 29 277
Fragments arranged by size:
1122 438 277 29
BsrBI CCG'CTC
Cuts at: 0 1596 1866
Size: 1596 270
BsrDI GCAATG_nn'
Cuts at: 0 1385 1866
Size: 1385 481
BsrFI r'CCGG_y
Cuts at: 0 1599 1866
Size: 1599 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 383 1137 1866
Size: 383 754 729
Fragments arranged by size:
754 729 383
BstEII G'GTnAC_C
Cuts at: 0 678 1866
Size: 678 1188
BstYI r'GATC_y
Cuts at: 0 1337 1622 1866
Size: 1337 285 244
Fragments arranged by size:
1337 285 244
BtgI C'CryG_G
Cuts at: 0 230 491 881 1866
Size: 230 261 390 985
Fragments arranged by size:
985 390 261 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 595 1866
Size: 595 1271
BtsI GCAGTG_nn'
Cuts at: 0 640 1215 1866
Size: 640 575 651
Fragments arranged by size:
651 640 575
DraIII CAC_nnn'GTG
Cuts at: 0 1440 1866
Size: 1440 426
DrdI GACnn_nn'nnGTC
Cuts at: 0 1198 1866
Size: 1198 668
EaeI y'GGCC_r
Cuts at: 0 558 827 1597 1866
Size: 558 269 770 269
Fragments arranged by size:
770 558 269 269
EagI C'GGCC_G
Cuts at: 0 1597 1866
Size: 1597 269
EarI CTCTTCn'nnn_
Cuts at: 0 1144 1717 1866
Size: 1144 573 149
Fragments arranged by size:
1144 573 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1810 1866
Size: 1810 56
EcoICRI GAG'CTC
Cuts at: 0 1062 1866
Size: 1062 804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 187 241 588 1060 1404 1423 1462
Size: 97 90 54 347 472 344 19 39
Cuts at: 1462 1540 1846 1866
Size: 78 306 20
Fragments arranged by size:
472 347 344 306 97 90 78 54
39 20 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1667 1866
Size: 1667 199
EcoO109I rG'GnC_Cy
Cuts at: 0 496 729 980 1200 1708 1866
Size: 496 233 251 220 508 158
Fragments arranged by size:
508 496 251 233 220 158
EcoRV GAT'ATC
Cuts at: 0 1322 1866
Size: 1322 544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1746 1866
Size: 1746 120
FspI TGC'GCA
Cuts at: 0 154 1273 1866
Size: 154 1119 593
Fragments arranged by size:
1119 593 154
FspAI rTGC'GCAy
Cuts at: 0 1273 1866
Size: 1273 593
HaeII r_GCGC'y
Cuts at: 0 672 1866
Size: 672 1194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 901 933 1018 1050 1700 1732
Size: 739 32 130 32 85 32 650 32
Cuts at: 1732 1866
Size: 134
Fragments arranged by size:
739 650 134 130 85 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 295 1866
Size: 295 1571
KasI G'GCGC_C
Cuts at: 0 668 1866
Size: 668 1198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1866
Size: 1310 556
MscI TGG'CCA
Cuts at: 0 560 1866
Size: 560 1306
MslI CAynn'nnrTG
Cuts at: 0 1813 1866
Size: 1813 53
MspA1I CmG'CkG
Cuts at: 0 24 117 760 768 1196 1742 1866
Size: 24 93 643 8 428 546 124
Fragments arranged by size:
643 546 428 124 93 24 8
NarI GG'CG_CC
Cuts at: 0 669 1866
Size: 669 1197
NspI r_CATG'y
Cuts at: 0 79 308 358 1122 1644 1816 1866
Size: 79 229 50 764 522 172 50
Fragments arranged by size:
764 522 229 172 79 50 50
PasI CC'CwG_GG
Cuts at: 0 1235 1866
Size: 1235 631
PciI A'CATG_T
Cuts at: 0 1640 1812 1866
Size: 1640 172 54
Fragments arranged by size:
1640 172 54
PfoI T'CCnGG_A
Cuts at: 0 1064 1866
Size: 1064 802
PpuMI rG'GwC_Cy
Cuts at: 0 496 980 1200 1708 1866
Size: 496 484 220 508 158
Fragments arranged by size:
508 496 484 220 158
PstI C_TGCA'G
Cuts at: 0 1571 1866
Size: 1571 295
PvuII CAG'CTG
Cuts at: 0 24 760 768 1866
Size: 24 736 8 1098
Fragments arranged by size:
1098 736 24 8
SacI G_AGCT'C
Cuts at: 0 1064 1866
Size: 1064 802
SanDI GG'GwC_CC
Cuts at: 0 496 1708 1866
Size: 496 1212 158
Fragments arranged by size:
1212 496 158
SapI GCTCTTCn'nnn_
Cuts at: 0 1717 1866
Size: 1717 149
SbfI CC_TGCA'GG
Cuts at: 0 1571 1866
Size: 1571 295
SexAI A'CCwGG_T
Cuts at: 0 1294 1866
Size: 1294 572
SfcI C'TryA_G
Cuts at: 0 454 512 575 1567 1866
Size: 454 58 63 992 299
Fragments arranged by size:
992 454 299 63 58
SfoI GGC'GCC
Cuts at: 0 670 1866
Size: 670 1196
SmlI C'TyrA_G
Cuts at: 0 287 1866
Size: 287 1579
StuI AGG'CCT
Cuts at: 0 208 1866
Size: 208 1658
StyI C'CwwG_G
Cuts at: 0 725 1126 1866
Size: 725 401 740
Fragments arranged by size:
740 725 401
TatI w'GTAC_w
Cuts at: 0 257 1866
Size: 257 1609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 160 175 192 207 423 455 677 709
Size: 160 15 17 15 216 32 222 32
Cuts at: 709 799 831 1153 1185 1802 1834 1866
Size: 90 32 322 32 617 32 32
Fragments arranged by size:
617 322 222 216 160 90 32 32
32 32 32 32 17 15 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 1866
Size: 138 1728
XhoI C'TCGA_G
Cuts at: 0 287 1866
Size: 287 1579
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AleI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI
NspI PasI PciI PfoI PpuMI PstI PvuII SacI
SanDI SapI SbfI SexAI SfcI SfoI SmlI StuI
StyI TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbsI BcgI
BfrBI BlpI BmtI BpuEI BsaI BsaAI BsiWI BsrGI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I ClaI CspCI
DraI EcoRI FseI HincII HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XmaI XmnI ZraI