################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 482 AAATTT [GA]^AATT[CT] AcyI 367 GACGCC G[GA]^CG[CT]C Alw44I 560 GTGCAC G^TGCAC Aor51HI 775 AGCGCT AGC^GCT ApaLI 560 GTGCAC G^TGCAC ApoI 482 AAATTT [GA]^AATT[CT] BbsI 199 GTCTTC GAAGAC(2/6) BbvCI 418 CCTCAGC CCTCAGC(-5/-2) BlpI 89 GCTCAGC GC^T[ACGT]AGC Bpu1102I 89 GCTCAGC GC^T[ACGT]AGC BpuAI 199 GTCTTC GAAGAC(2/6) BsaHI 367 GACGCC G[GA]^CG[CT]C BsiEI 1425 CGGTCG CG[GA][CT]^CG BsmAI 459 GAGAC GTCTC(1/5) BsmFI 415 GTCCC GGGAC(10/14) BsmI 279 GAATGC GAATGC(1/-1) Bsp1407I 875 TGTACA T^GTACA BsrBI 1386 CCGCTC CCGCTC(-3/-3) BsrGI 875 TGTACA T^GTACA BssSI 1063 CACGAG CACGAG(-5/-1) BstAPI 423 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 449 AGATCC [GA]^GATC[CT] CelII 89 GCTCAGC GC^T[ACGT]AGC DraII 881 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 500 TGGCCG [CT]^GGCC[GA] Eco47III 775 AGCGCT AGC^GCT EcoO109I 881 AGGCCCT [GA]G^G[ACGT]CC[CT] Hin1I 367 GACGCC G[GA]^CG[CT]C HincII 1252 GTCAAC GT[CT]^[GA]AC HindII 1252 GTCAAC GT[CT]^[GA]AC MfeI 400 CAATTG C^AATTG MflI 449 AGATCC [GA]^GATC[CT] MslI 563 CACTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 400 CAATTG C^AATTG NlaIV 493 GGGGCC GG[ACGT]^[ACGT]CC SapI 642 GCTCTTC GCTCTTC(1/4) SspBI 875 TGTACA T^GTACA XhoII 449 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BfaI 886 CTAG C^TAG 1081 CTAG BglI 502 GCCGTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 883 GCCCTAGAGGC BsrI 393 CCAGT ACTGG(1/-1) 1028 CCAGT EarI 643 CTCTTC CTCTTC(1/4) 1381 CTCTTC HaeII 775 AGCGCT [GA]GCGC^[CT] 1186 AGCGCC HgaI 367 GACGC GACGC(5/10) 970 GACGC Ksp632I 643 CTCTTC CTCTTC(1/4) 1381 CTCTTC MaeI 886 CTAG C^TAG 1081 CTAG MseI 743 TTAA T^TAA 1321 TTAA SacI 259 GAGCTC GAGCT^C 640 GAGCTC SfaNI 670 GATGC GCATC(5/9) 1007 GCATC StuI 323 AGGCCT AGG^CCT 1377 AGGCCT TaqI 329 TCGA T^CGA 577 TCGA Tru9I 743 TTAA T^TAA 1321 TTAA Tsp509I 401 AATT ^AATT 483 AATT TthHB8I 329 TCGA T^CGA 577 TCGA XcmI 145 CCAGAGGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 497 CCATGGCCGTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI Tsp45I TspRI