(Circular) (Six-base) MAPSORT of: pFN21AE1949.seq Check: 4241 from: 1 to: 6217 pFN21AE1949 6217 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2590 2683 2590 Size: 93 6124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4280 5664 5932 1632 Size: 2648 1384 268 1917 Fragments arranged by size: 2648 1917 1384 268 AfeI AGC'GCT Cuts at: 2782 2782 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 3514 5048 3514 Size: 1534 4683 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2590 1184 Size: 1406 4811 AlwNI CAG_nnn'CTG Cuts at: 2254 2836 3057 3960 5532 2254 Size: 582 221 903 1572 2939 Fragments arranged by size: 2939 1572 903 582 221 ApaLI G'TGCA_C Cuts at: 2565 4274 5430 2565 Size: 1709 1156 3352 Fragments arranged by size: 3352 1709 1156 ApoI r'AATT_y Cuts at: 1493 2487 3526 3752 3904 6013 1493 Size: 994 1039 226 152 2109 1697 Fragments arranged by size: 2109 1697 1039 994 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3542 1870 Size: 73 1599 4545 Fragments arranged by size: 4545 1599 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3149 3182 3149 Size: 33 6184 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3538 4993 618 Size: 325 217 627 1751 1455 1842 Fragments arranged by size: 1842 1751 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2268 2649 3140 3268 3444 3536 1391 Size: 877 381 491 128 176 92 4072 Fragments arranged by size: 4072 877 491 381 176 128 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2197 928 Size: 971 298 4948 Fragments arranged by size: 4948 971 298 BbvCI CC'TCA_GC Cuts at: 2424 2424 Size: 6217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2513 2894 Size: 107 122 71 631 605 337 504 381 Cuts at: 2894 4828 136 Size: 1934 1525 Fragments arranged by size: 1934 1525 631 605 504 381 337 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2095 3634 2095 Size: 1539 4678 Bme1580I G_kGCm'C Cuts at: 1790 2135 2569 2830 4278 5434 1790 Size: 345 434 261 1448 1156 2573 Fragments arranged by size: 2573 1448 1156 434 345 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2381 3172 3352 3496 4877 1239 Size: 1139 3 791 180 144 1381 2579 Fragments arranged by size: 2579 1381 1139 791 180 144 3 Bpu10I CC'TnA_GC Cuts at: 2424 2424 Size: 6217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 2453 2480 4342 5207 5505 2150 Size: 303 27 1862 865 298 2862 Fragments arranged by size: 2862 1862 865 303 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2373 4024 4406 5041 Size: 53 83 186 936 840 1651 382 635 Cuts at: 5041 275 Size: 1451 Fragments arranged by size: 1651 1451 936 840 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3514 4650 5048 5322 5469 5880 3514 Size: 1136 398 274 147 411 3851 Fragments arranged by size: 3851 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2864 2894 3147 3177 1178 Size: 30 165 30 1461 30 253 30 4218 Fragments arranged by size: 4218 1461 253 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2032 2035 2362 2430 1186 Size: 846 3 327 68 4973 Fragments arranged by size: 4973 846 327 68 3 BseYI C'CCAG_C Cuts at: 2247 2272 2665 3109 3139 3145 5420 5916 2247 Size: 25 393 444 30 6 2275 496 2548 Fragments arranged by size: 2548 2275 496 444 393 30 25 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 3667 4428 5456 1540 Size: 275 186 1432 234 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2268 2295 2569 2649 2723 3203 4278 4363 5434 Size: 27 274 80 74 480 1075 85 1071 Cuts at: 5434 2268 Size: 3051 Fragments arranged by size: 3051 1075 1071 480 274 85 80 74 27 BsmI GAATG_Cn' Cuts at: 2290 3728 3821 2290 Size: 1438 93 4686 Fragments arranged by size: 4686 1438 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2268 2295 2569 2649 2723 2830 Size: 399 345 133 27 274 80 74 107 Cuts at: 2830 3140 3203 3268 3444 3536 4278 4363 5434 Size: 310 63 65 176 92 742 85 1071 Cuts at: 5434 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 345 310 274 176 133 107 92 85 80 74 65 63 27 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2590 2683 3572 844 Size: 1229 517 93 889 3489 Fragments arranged by size: 3489 1229 889 517 93 BsrBI CCG'CTC Cuts at: 1394 1823 3393 4104 1394 Size: 429 1570 711 3507 Fragments arranged by size: 3507 1570 711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3514 4861 5048 1700 Size: 110 1704 1347 187 2869 Fragments arranged by size: 2869 1704 1347 187 110 BsrGI T'GTAC_A Cuts at: 96 2880 96 Size: 2784 3433 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 3068 4277 5289 3068 Size: 1209 1012 3996 Fragments arranged by size: 3996 1209 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 794 Size: 1640 4577 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2084 2242 2909 2084 Size: 158 667 5392 Fragments arranged by size: 5392 667 158 BstYI r'GATC_y Cuts at: 1972 2454 3547 3914 4299 4316 5768 5854 5866 Size: 482 1093 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1093 482 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2502 2821 2902 3487 513 Size: 552 939 498 319 81 585 3243 Fragments arranged by size: 3243 939 585 552 498 319 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2788 2951 487 Size: 683 56 1562 163 3753 Fragments arranged by size: 3753 1562 683 163 56 BtsI GCAGTG_nn' Cuts at: 2985 3733 4527 4547 2985 Size: 748 794 20 4655 Fragments arranged by size: 4655 794 748 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3664 4553 Size: 54 1425 50 275 18 675 1159 889 Cuts at: 4553 6182 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1159 889 675 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2654 3392 4147 1353 Size: 1301 738 755 3423 Fragments arranged by size: 3423 1301 755 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2743 4804 5178 5324 387 Size: 1064 138 1154 2061 374 146 1280 Fragments arranged by size: 2061 1280 1154 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2266 2647 2266 Size: 381 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2381 3172 3352 3496 4280 4877 Size: 393 746 3 791 180 144 784 597 Cuts at: 4877 5664 5932 1239 Size: 787 268 1524 Fragments arranged by size: 1524 791 787 784 746 597 393 268 180 144 3 EcoO109I rG'GnC_Cy Cuts at: 2887 2887 Size: 6217 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2784 3195 5364 1678 Size: 1106 411 2169 2531 Fragments arranged by size: 2531 2169 1106 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373 Size: 32 135 32 3286 32 42 32 2626 Fragments arranged by size: 3286 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3259 3561 3807 669 Size: 988 281 1321 302 246 3079 Fragments arranged by size: 3079 1321 988 302 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 2405 3816 2405 Size: 1411 4806 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 2850 5331 5515 1305 Size: 822 723 2481 184 2007 Fragments arranged by size: 2481 2007 822 723 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2572 4175 4534 4693 518 Size: 648 1406 1603 359 159 2042 Fragments arranged by size: 2042 1603 1406 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2251 2281 2434 2611 2665 2836 3109 Size: 554 91 30 153 177 54 171 273 Cuts at: 3109 3149 3633 4310 5458 5703 1606 Size: 40 484 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 554 484 273 245 177 171 153 91 54 40 30 NcoI C'CATG_G Cuts at: 513 1065 2004 2502 2821 2902 3487 513 Size: 552 939 498 319 81 585 3243 Fragments arranged by size: 3243 939 585 552 498 319 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3244 1348 Size: 1896 4321 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PfoI T'CCnGG_A Cuts at: 2355 2355 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3086 3118 3086 Size: 32 6185 PstI C_TGCA'G Cuts at: 830 2034 2328 3154 3336 3569 830 Size: 1204 294 826 182 233 3478 Fragments arranged by size: 3478 1204 826 294 233 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2160 2281 2434 2611 2665 3149 2160 Size: 121 153 177 54 484 5228 Fragments arranged by size: 5228 484 177 153 121 54 SacI G_AGCT'C Cuts at: 2268 2649 2268 Size: 381 5836 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2654 2654 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2030 2324 3150 3332 3565 4700 5381 Size: 219 985 294 826 182 233 1135 681 Cuts at: 5381 5572 826 Size: 191 1471 Fragments arranged by size: 1471 1135 985 826 681 294 233 219 191 182 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 2459 2468 4321 5222 5484 Size: 197 926 222 294 9 1853 901 262 Cuts at: 5484 820 Size: 1553 Fragments arranged by size: 1853 1553 926 901 294 262 222 197 9 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2330 3384 1517 Size: 813 1054 4350 Fragments arranged by size: 4350 1054 813 StyI C'CwwG_G Cuts at: 513 1065 2004 2376 2502 2821 2902 3487 3656 Size: 552 939 372 126 319 81 585 169 Cuts at: 3656 513 Size: 3074 Fragments arranged by size: 3074 939 585 552 372 319 169 126 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 4261 4414 4599 5044 325 Size: 1255 2664 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2880 4463 6175 Size: 274 80 33 51 1443 903 1583 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1712 1583 1443 903 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2692 2724 3147 3179 3260 3292 6177 6209 2692 Size: 32 423 32 81 32 2885 32 2700 Fragments arranged by size: 2885 2700 423 81 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2157 2509 2157 Size: 352 5865 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI