################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApoI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI MamI MboI MfeI MflI MluI MroI MseI MslI MunI NcoI NdeI NdeII NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 359 GGTACC G^GTACC AccI 868 GTCTAC GT^[AC][GT]AC Alw44I 292 GTGCAC G^TGCAC AlwNI 56 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 292 GTGCAC G^TGCAC AscI 701 GGCGCGCC GG^CGCGCC Asp718I 359 GGTACC G^GTACC AviII 398 TGCGCA TGC^GCA BalI 557 TGGCCA TGG^CCA BbeI 462 GGCGCC GGCGC^C BbvCI 471 CCTCAGC CCTCAGC(-5/-2) BfaI 1016 CTAG C^TAG BpmI 505 CTGGAG CTGGAG(16/14) BsaWI 1098 ACCGGA [AT]^CCGG[AT] BsmBI 1004 GAGACG CGTCTC(1/5) BsrBI 862 CCGCTC CCGCTC(-3/-3) BsrDI 101 GCAATG GCAATG(2/0) BsrFI 571 GCCGGC [GA]^CCGG[CT] BsrI 24 ACTGG ACTGG(1/-1) BstAPI 375 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 571 GCCGGC [GA]^CCGG[CT] DdeI 472 CTCAG C^T[ACGT]AG EarI 454 CTCTTC CTCTTC(1/4) FspI 398 TGCGCA TGC^GCA KasI 462 GGCGCC G^GCGCC KpnI 359 GGTACC GGTAC^C Ksp632I 454 CTCTTC CTCTTC(1/4) KspI 650 CCGCGG CCGC^GG MaeI 1016 CTAG C^TAG MaeII 554 ACGT A^CGT MaeIII 410 GTAAC ^GT[ACGT]AC MluNI 557 TGGCCA TGG^CCA MscI 557 TGGCCA TGG^CCA NaeI 571 GCCGGC GCC^GGC NarI 462 GGCGCC GG^CGCC NgoMIV 571 GCCGGC G^CCGGC NotI 923 GCGGCCGC GC^GGCCGC PstI 127 CTGCAG CTGCA^G SacI 508 GAGCTC GAGCT^C SacII 650 CCGCGG CCGC^GG SapI 453 GCTCTTC GCTCTTC(1/4) SfcI 127 CTGCAG C^T[GA][CT]AG SfoI 462 GGCGCC GGC^GCC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BglI 706 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 986 GCCCACCCGGC BsmAI 1004 GAGAC GTCTC(1/5) 346 GTCTC BsmFI 1065 GGGAC GGGAC(10/14) 647 GTCCC BspMI 296 ACCTGC ACCTGC(4/8) 578 ACCTGC HaeII 450 GGCGCT [GA]GCGC^[CT] 462 GGCGCC HphI 597 GGTGA GGTGA(8/7) 311 TCACC MboII 80 TCTTC GAAGA(8/7) 455 TCTTC MspA1I 379 CCGCTG C[AC]G^C[GT]G 650 CCGCGG NlaIII 315 CATG CATG^ 708 CATG PpuMI 645 GGGTCCC [GA]G^G[AT]CC[CT] 1065 GGGACCC SfaNI 7 GATGC GCATC(5/9) 256 GATGC XcmI 242 CCATCTACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 707 CCATGCTGGCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI TseI TspRI